4 19003572427424163 PDF
4 19003572427424163 PDF
4 19003572427424163 PDF
FEATURE
ARTICLE The Automated Hematology Analyzer,
the Pentra 80
Franck SEGUY*
*ABX S.A.
Abstract
Hematology diagnostics is growing increasingly important as one of the most fundamental types of clinical testing.
ABX-Horiba group has been developing and marketing a variety of hematology analyzers based on the key concept of
providing “Quick, easy and precise testing”. The Pentra 80 is a compact, high-precision CBC + 5-DIFF automatic blood
analyzer. The author describe the measurement principles and features, such as the DHSSTM and MDSSTM , and also,
present some typical data that show the high performance of this instrument.
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FEATURE ARTICLE The Automated Hematology Analyzer, the Pentra 80
3 Specifications
Improving diagnosis quality and laboratory productivity
Diluent is a constraint shared by all biologists world-wide. The
Air Pentra 80 is designed to meet these requirements with
7µL discarded highly innovative technical specifications.
10µL WBC/BASO
10µL RBC/Plt/Hgb
3µL discarded (1) Measurement parameter & Result processing
The Pentra 80 can measure the complete blood-cell count
and differential leukocyte count with 26 parameters. And
provide affluent result processing functions (Fig.4).
ABSORBANCE
Pretreatment of a sample as follows:
* Sampling : only 53µL whole blood for CBC and DIFF.
* Sample aliquot taken, transferred to analysis
chambers. Pre-heated analysis chambers for highly
reproducible result.
* Tangential flow reagent dilution for optimal
homogenization.
NEUTROPHILS
Fig.2 Multi-Distribution Sampling System.
EOSINOPHILS
2.2 DHSSTM Technology
The DHSSTM Technology (Fig.3) is ABX’s original
LYMPHOCYTES
technology that combines the focused flow impedance
method, light absorbance measurement and
MONOCYTES
cytochemistry to measure the cell volume and cellular
content in one unique flow cell.
ATYPICAL
LYMPHOCYTES(ALY)
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LARGE IMMATURE
CELLS(LIC)
· 26 parameters.
· Histogram of RBC, WBC, PLT.
(1) Cytochemistry · Color leukocyte matrix.
The whole blood sample is incubated in a · Pathological and morphological alarms.
temperature-controlled chamber and enzymatic
· Differential leukocyte count by DHSS technology.
staining with Chlorazol Black. This reagent
specifically stains leukocyte nuclei, granules and · Basophil measurement through specific channel.
membranes. · Percentage and absolute value of neutrophils,
eosinophils, basophils, lymphocytes and monocytes.
(2) Cytometry
· Determination of 2 additional sub-populations (% and #):
And the prepared sample is injected into a double
hydrofocus cytometer (ABX patent) and analysis of Atypical lymphocytes (ALY), Large immature cells (LIC).
cell complexity with a polychromatic light source.
Fig.4 Results.
Fig.3 Double Hydrodynamic Sampling System.
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Technical Reports
4 Internal Evaluation
We have confirmed high performance of the Pentra 80
by following two ways.
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FEATURE ARTICLE The Automated Hematology Analyzer, the Pentra 80
5 Conclusion
The compact Pentra 80 hematology analyzer can
provide five-part differential white blood cell analysis,
contributing to the utilization of real-time testing.
Furthermore, with micro-sampling and quiet operation,
these products have been designed not only for
clinicians, but with the patient’s quality of life in mind as
well. We hope that this product will continue to make
further contributions to the field of medical treatment.
Franck SEGUY
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