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    Harvesting Multi-Cellular Microtissues From The 3D Petri Dish

    Uploaded by

    Maria Elena Maldonado

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Harvesting Multi-Cellular Microtissues From The 3D Petri Dish

    Uploaded by

    Maria Elena Maldonado
    13 views2 pages

    Original Title

    harvesting-multi-cellular-microtissues-from-3d-petri-dishes

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    Copyright:

    © All Rights Reserved
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    Download as pdf or txt
    13 views2 pages

    Harvesting Multi-Cellular Microtissues From The 3D Petri Dish

    Uploaded by

    Maria Elena Maldonado

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    Harvesting Multi-cellular Microtissues from the 3D Petri Dish®

    1. Remove cell culture medium surrounding the 3D Petri Dish® containing the multi-cellular

    microtissues.

    2. Place fresh cell culture medium into the well of a new 12 or 24 well plate depending on the

    size of the 3D Petri Dish® used (1.0mL for 12 series or 0.5ml for 24 series).

    3. Carefully remove the 3D Petri Dish® with a sterile spatula or sterile tweezers. Invert the 3D

    Petri Dish® into the well of the new plates. Be careful to not create air bubbles between the

    upside down 3D Petri Dish® and the bottom of the new culture plate. Cover the plate and

    place the 6-well plate in a swinging bucket rotor in a centrifuge and spin for 5 minutes at 500

    rpm.

    4. After centrifugation, view the inverted 3D Petri Dish® under the microscope to ensure that

    the multi-cellular microtissues have been released.

    5. Remove the empty 3D Petri Dish® with a sterile spatula or sterile tweezers.

    6. The harvested multi-cellular microtissues remaining in the cell culture medium can be

    washed and further processed depending on the application. (Immuno-staining, Western blot,

    RT-PCR, etc).

    For Research Use Only, Not for Clinical, Diagnostic or Therapeutic Use.

    www.microtissues.com
    3D Petri Dishes® and the micro-molds used to cast them are sold and distributed for evaluation
    and/or research purposes only, and are not intended for manufacturing, re-sale, human or
    animal diagnostic or therapeutic use.

    Notice to Purchaser - Label License


    For research use only. This product and/or its use falls within the scope of the claims of pending and allowed claims of
    U.S. Patent Applications 12/087, 937 and 61/249, 308 and their corresponding foreign applications.

    The purchase of this product conveys to the buyer the non-transferable right to use the product and components of the
    product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell
    or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a
    third party or otherwise use this product or its components or materials made using this product or its components for
    Commercial Purposes. The buyer may transfer information or materials made through the use of this product to a
    scientific collaborator, provided that a copy of this limited use label license is given to the collaborator, such transfer is
    not for any Commercial Purpose, and that such collaborator agrees in writing (a) not to transfer such materials to any
    third party, and (b) to use such transferred materials and/or information solely for research and not for Commercial
    Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to:
    (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service,
    information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4)
    resale of the product or its components, whether or not such product or its components are resold for use in research. For
    products that are subject to multiple limited use label licenses, the terms of the most restrictive limited use label license
    shall control. If the buyer is not willing to accept the limitations of this limited use label license, MicroTissues, Inc. is
    willing to accept return of the product with a full refund.

    For information on purchasing a license to this product for purposes other than research, contact MicroTissues, Inc.,
    [email protected].

    All trademarks herein are marks of MicroTissues, Inc.

    For Research Use Only, Not for Clinical, Diagnostic or Therapeutic Use.

    www.microtissues.com

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