REVIEW ARTICLE

J. Food Sci. Techol. Nepal, Vol. 9 (1-10), 2016

ISSN: 1816-0727

Interactions of Bio-macromolecules During Processing of Instant Asian

Noodles: A Review
PRATIKSHA SHRESTHA*1, KRISHNA PRASAD RAI2 and ANIL KUMAR ANAL1
1
Food Engineering and Bio Process Technology (FEBT), Asian Institute of Technology, Pathumthani 12120, Thailand
2
Department of Food Technology and Quality Control, Babarmahal, Kathmandu, Nepal

The review focuses on how the protein, fats, carbohydrates and water interact and form the complexes during different stages
of processing of instant noodles. Moisture is needed for dough formation and chemical interactions therein. Appropriate
moisture content is required for proper gelatinization of noodle strand during steaming and eventually moisture is evaporated
off by drying and frying to increase its shelf life. Frying oil may undergo degradation process invited by high frying time and
temperature, high moisture in oil and polyvalent metals. Various intermediate products like dimers and polymers accelerate
the oil degradation finally changing the physiochemical properties of oil rendering it unacceptable for processing. Gliadin
and glutenin gives noodle its viscoelastic character. The polymeric glutenin network develops throughout the process of
mixing, resting and subsequent stages of sheeting. Protein content and amylose content in noodle are inversely proportional
to the oil uptake ratio. Another possibility is the non-enzymatic browning reaction during frying of instant noodles.

Keywords: Instant noodles, Biochemical reaction, Nutritional aspects, Processing, Quality

Introduction There is no any approved international standard method for

preparation of instant Asian noodles. However, there are basic
Instant noodles are ready to serve with easy preparation. guidelines as shown in Figure 1, which needs to be followed
There are two main types of instant noodles: a) steamed and for maintaining quality characteristics of Asian Noodles.
deep-fried and b) steamed and air-dried pre-cooked instant Preparation of salt solution
noodles need only 3–4 min of boiling or rehydration with (Solution of Sodium carbonate,
boiling water to prepare for serving. The frying process Phosphate salts and gums)
removes the water from the noodle strands, resulting in a Flour (14% M.C.)
porous structure that rehydrates quickly when hot water is
added. Determination of flour particle size and water absorption

Determination of optimum water addition for dough make-up

Asian Noodles can be broadly divided into two classes a)
salted noodles and b) yellow alkaline noodles. The most Dough mixing (Horizontal or vertical mixer)
apparent difference between the two types is the addition of
Dough Resting (15-20 min)
alkali (sodium and potassium carbonate). The yellow color
of the noodle is due to the chemical reaction of flavones Sheeting
under alkaline condition. Asian noodles are generally Compound Sheeting: combine 2 sheets between pair of rolls
made from durum wheat flour (Cuesta, et al., 1993) as it
gives superior color and better texture (Fu, 2006). Durum Reduction sheeting: until sheet is within specification. (± 0.03 -0.05mm)
wheat is hard wheat containing high protein. According to Slitting, waving: specified noodle width according to type of noodle
(CAC, 2016), durum wheat flour shall not contain less than
11% protein. The gluten protein the elasticity character. Steaming 100 ˚C, 1-5 minutes
Increasing the protein content of flour also contributes to
Frying at 130-160 ˚C, 1-3 minutes uses palm oil or palmolein
firmness (Karim, 1990).
Figure 1. Guidelines for Instant Asian Noodles making
process (Ross, 2005)

*Corresponding author, E-mail: [email protected]

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Noodles represent a complex system in which multiple common. Optimal firmness for instant noodles is observed
components interact to achieve final characteristic product. with either 0.215% kansui or 2.0% NaCl. However, the
The interactions or combined effects of water, starch protein addition of kansui reduced noodle nutritional quality, and
and lipid under various conditions are the components of high levels of both table (2.03.0%) and alkaline (1.001.5%)
major concern in the processing and product quality of salt increased cooking losses (Rombouts, et al., 2014).
Asian noodles. Dough and noodle quality is affected by Powders of black soda are dissolved in water to prepare the
wheat proteins (Gary, 2013), starch (Noda et al., 2001), solution. The chemical ingredients are dissolved in water
lipids (Lu, et al., 2009), and enzymes (Fu, 2008). (soft water) and stored in storage tank. The pH of the mixing
water is maintained at 7.2 – 7.5 at 20˚C during storage.
Protein, glycans and lipids are the major bio-macromolecules Likewise, salt solution is prepared in separate tank and fed
involved in noodle processing. Bio-macromolecules are into the mixing batch at continuous rate.
the biopolymers formed by joining together small building
blocks (bio-molecular monomers) in the cells (Tsai, 2007). In noodle making, incorporation of water with added
There are mainly 3 true macromolecules; carbohydrate, ingredients is carried out such that actual dough development
protein and nucleic acid. Lipid is not truly a macromolecule. is minimized. Furthermore, absorption of water is only 29
Monomer constituents of bio-macromolecules are mono- – 30 percent. The incorporation of water is mainly done
saccharides (for glycans), amino acid (for protein), and to have enough water to hydrate the flour. Less moisture
glycerol and fatty acid (for lipid). For bio-macromolecules, content of dough means that there will be less water to
there is usually a tetrahedral arrangement of bonds around eliminate during subsequent steps of drying and frying. The
single-bonded carbon atoms and phosphorus atoms. Organic water absorption is dependent on the type of flour. Water
molecules, including bio-macromolecules, are held together temperature affects mixing time and noodle quality. If
with covalent bonds. Their 3D structures are characterized water temperature is too low (<18 ˚C), it slows down flour
by bond lengths, bond angles and rotations of groups of hydration speed and gluten development and requires a
atoms about the bonds Covalent bonds are the major source longer mixing time. If water temperature is too high (>30
of energy for cells when broken. Computer graphics provides ˚C), excessive heat generated during mixing could denature
the most comprehensive tool for depicting 3 dimensional the protein and gelatinize the starch, resulting in sticky
structures of biomolecules. dough. High dough temperature can also increase enzyme
activity and deteriorate noodle quality. It is recommended
Competition of protein and starch for water during that the water temperature be adjusted to achieve a final
mixing dough temperature of 25-30 ˚C (Hou, 2011).
During dough mixing, proteins, pentosans and starch Dough development: lipid, protein and starch
compete for the limited amount of water available. Since interactions
the starch comprises at least 70 % of the flour, it will absorb
a substantial amount of the water. Starch which has been Unlike bread making, dough development is not fully
damaged in the milling process has an even greater affinity achieved while preparing dough for noodles. In noodle
for water absorbing up to three times as much water as dough, proteins do not pull away from starch granules during
undamaged starch (Kruger, 1996). mixing. The micro structure resembles that of compacted
flour, which is due to the low moisture content of noodle
In Asian noodles, alkali is added in water to increase water dough (Moss, 1987). During noodle dough mixing, protein
absorption and decreases the mixing requirement of wheat absorbs water and forms a limited gluten network, which
flour dough. Larger increase in water absorption is with glues other wheat flour components together, resulting
sodium hydroxide than with either sodium or potassium in crumbly dough. Presence of protein–carbohydrate
carbonates (Moss, 1986). Alkali addition also affects the complexes in gluten (McMaster, 1983), binding of lipids to
gelatinization properties of starch. For example; sodium gluten proteins during dough mixing (Bekes, et al., 1983)
hydroxide can gelatinize starch at room temperature and implication of both carbohydrate and lipid complex
(Maher, 1983). Changes in dough properties under alkaline formation with glutenin proteins (Zawistowska, 1985) all
condition have been attributed to oxidation of sulfhydryl- directly or indirectly support the possible roles of starch
disulfide exchange and oxidation of globulin fraction to granular proteins and lipids in starch– gluten interactions.
form high molecular weight proteins (Terada, 1978). Within a dough system, starch exists primarily in granular
Alkali can be powder which is also called “soda ash”or form, while protein is developed into a continuous three-
liquid form that is termed as “lye water”. In Japan, mixture dimensional network.
of sodium and potassium carbonate (kansui) are more

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Scanning electron microscope studies would indicate not complete even at the end of the compression stage. The
that there is some disaggregation and mixing of protein reduction stage which follows further develops the gluten.
component, largely responsible for dough development, but Uniform protein matrix is obtained at this stage (Moss,
that is generally incomplete with some vestiges of intact 1987).
cell components remaining (Moss, 1987). The viscous
flow aspect of dough is known to be due to the monomeric The development and alignment of the gluten in the dough
gliadin component, whereas the polymeric glutenin gives sheet allows noodle strands to be slit from the dough sheet
the elasticity. Gluten is the important gas retaining protein. without breakage or loss of shape during drying, frying
Concerning the importance of gliadin and glutenin subunits, and cooking process. Gluten protein content is negatively
both aggregate and individual (Kruger, 1996). correlated with cooking loss, and positively with noodle
tensile strength and firmness (Hou, 2013).The stage of
Excessively elastic gluten tends to produce a tough dough dough resting helps moisture distribute more evenly. This
sheet, which shrinks back during the sheeting process, also enhances disulfide bond formation and forms bond
making it difficult to reduce the thickness and extend the between gluten and lipid. Whether a dough sheet is allowed
length of the dough sheet, and making noodle strands non- to rest or not has a significant impact on the degree of starch
pliable. On the other hand, surface peeling and tearing of gelatinization during steaming. According to (Wu, 1998)
the sheet could occur during sheeting through multiple well-rested dough has a higher degree of starch gelatinization
rolls when the rate of gluten development is too slow or the than unrested dough, as examined by differential scanning
developed gluten network is too small or weak to provide calorimetric. The lack of evenly distributed water may
complete encapsulation of flour components. Protein prevent starch from being fully gelatinized during steaming.
component also determines the firmness of noodle (Ohio, Meanwhile, the unrelaxed gluten may suppress starch
1985). swelling.

Interaction of water on resting Starch gelatinization and protein denaturation on

steaming
After completion of mixing, dough is allowed to rest to
achieve proper distribution of water. Dough is rested in a Noodles are subjected to steam to partially gelatinize and
receiving container while being stirred slowly (5–10 rpm). swell the starch which is needed for fast rehydration rate of
The stirring helps to dissipate heat inside the dough mass, the finished product before serving. Starch does not begin
prevent formation of large dough lumps, and feed dough to gelatinize until it reaches 84˚C even when the moisture is
crumbs into the sheeting rolls in a continuous process. more than 70% (Wu et al., 1998). The starch gelatinization
temperature of noodles is therefore even higher than
Resting produces smoother dough sheet as the protein 84˚C. Generally, saturated steam is used for steaming the
mellows and becomes more extensible. Examination of noodles. Drop in temperature results in condensation of
dough microstructure shows rested dough has more uniform steam. To overcome this defect superheated steam can also
protein matrix and fewer air spaces (Moss, 1987). However, be used. Superheated steam is the steam with additional
there is no change in the textural properties of adhesiveness, sensible heat to raise its temperature above corresponding
springiness and cohesiveness for noodles processed by saturation temperature at given pressure. When processing
superheated steam (Pronyk, 2008).Larger flour particle will with superheated steam, noodles dry rapidly and become
take more time to incorporate water and thus having fairly rapidly cooked. There is also much more gelatinization of
fine particle size is beneficial. The most important thing starch when using superheated steam than saturated steam
affecting water absorption is the addition of alkali and salt. (Pronyk, 2008).Steaming denatures the protein and helps
to fix the noodle waves. The protein denaturation process
Dough resting is also carried out in sheeting and size
usually occurs prior to starch gelatinization because of
reduction as well by slowing down the speed of multilayer
relatively low moisture in noodles (40% moisture).
conveyor belt which is aided by temperature controlled and
relative humidity controlled cabinet. Passing the dough Moisture loss during frying
sheets through a series of rolls with reduced roller gaps,
gluten is further developed and aligned to the sheeting Frying of instant noodles is done at 140–1600C hot oil for
direction with further encapsulation of starch granules. The 1-2 minutes. During the process, water vaporizes quickly
microstructure during the compression stage indicates that form the surface of the noodle. Dehydration of outer surface
adjacent endosperm particles become fused together so that of noodle drags water to migrate from the interior to the
the protein matrix within one endosperm particle becomes surface resulting porous spongy structure. These holes serve
continuous with that of adjacent particles. However, only as channels for water to get in upon rehydration in hot water.
a few sub aleurone protein masses show orientation in Eventually, some of the water in noodles is replaced by oil.
noodle dough and the fusion of the endosperm particle is
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Because the surfaces of fried noodle strands absorb oil during Water, steam, and oxygen initiate the chemical reactions in
frying, they can easily be separated in hot water. It usually the frying oil and food. Water, a weak nucleophile, attacks
takes 3–4 minutes to cook or soak fried instant noodles in the ester linkage of triacylglycerols and produces di and
hot water before consumption. The moisture content of the monoacylglycerols, glycerol, and free fatty acids. Free fatty
finished noodle products is in the range of 3–7%. The main acids contents in frying oil increase with the number of
purpose of frying instant noodle is to remove excess water fryings (Chung, et al., 2004). Stevenson and others (1984)
from the noodles, which causes weight loss accounts for 30 suggested that the maximum free fatty acid content for frying
-32% of the noodles. oil is 0.05% to 0.08%. Antioxidant decreases the frying oil
oxidation, but the effectiveness of antioxidant decreases
Thermal oxidation of lipid with high frying temperature (Min, 2007). Antioxidants,
Tertiary Butyl Hydroxy Quinone (TBHQ) is mostly used
Series of complex reactions such as oxidation, hydrolysis,
for palm oil and delta Tocopherol for beef tallow. It is well
isomerization and polymerization take place during the
known that TBHQ shows greater stability than Butylated
deep-fat frying that influence quality attributes of the
Hydroxy Quinone (BHQ) (Rho, 1986).
final product such as flavor, texture, shelf life and nutrient
composition (Zhang, 2012). Saturated fat such as palm oil, Decomposition of ester linkage takes place when water
beef tallow, vanaspati ghee etc. are used for deep frying and heat are concomitant to each other (Houhoula, 2003).
of instant noodles in the industry. The reactions in deep Thermal hydrolysis takes place mainly within the oil phase
fat- frying depends upon factors such as replenishment of rather than water–oil interface (Lascaray, 1949). Hydrolysis
fresh oil, frying conditions, original quality of frying oil, is more preferable in oil with short and unsaturated fatty acids
food materials, type of fryer, antioxidants, and oxygen than oil with long and saturated fatty acids because short and
concentration. High frying temperature, the number of unsaturated fatty acids are more soluble in water than long
frying, the contents of free fatty acids, polyvalent metals, and and saturated fatty acids. Water from foods is easily accessible
unsaturated fatty acids of oil decrease the oxidative stability to short-chain fats and oils for hydrolysis (Nawar, 1969).
and flavor quality of oil. The thermal oxidation rate is faster Water hydrolyzes the oil faster than steam (Pokorny, 1981).
than the autoxidation. The mechanism of thermal oxidation Frequent replacement of frying oil with fresh oil slows down
involves the initiation, propagation, and termination of the the hydrolysis of frying oil (Romero, 1998) while polymer
reaction as shown in Figure 2. compounds increases with number of frying as illustrated in
Figure 3. Di- and monoacylglycerols, glycerol, and free fatty
Initiation: CH3-(CH2)3-CH2-CH=CH-CH2-CH=CH- acids accelerate the further hydrolysis reaction of oil (Frega,
CH2(CH2)6-COOH 1999). Glycerol evaporates at 1500C and the remaining
↓- H glycerol in oil promotes the production of free fatty acids by
CH3-(CH2)4-CH-CH=CH-CH=CH-CH2(CH2)7-COOH hydrolysis (Naz, et al., 2005). Interestingly, Peroxide value
(PV) of frying oil also increases with increasing frying time
↓+O2
and temperature (> 180˚C) although at low temperature, PV
Propagation: CH3-(CH2)4-C̩ H-CH=CH-CH=CH- decreases with increasing time of frying oil Figure 4. Radical
CH2(CH2)7-COOH oxygen requires radical oil for the oxidation of oil. The
O̩ ↓ +Ḣ hydrogen with the weakest bond on the carbon of oil will
be removed first to become radical. The various strengths of
O
hydrogen carbon bond of fatty acids explain the differences
CH3-(CH2)4-C̩ H-CH=CH-CH=CH-CH2(CH2)7-COOH of oxidation rates of stearic, oleic, linoleic, and linoleic acids
O̩ ↓ -OḢ during thermal oxidation or autoxidation. The polyvalent
OH metals as Fe3+ and Cu2+ remove hydrogen protons from oil
to form alkyl radicals by oxidation-reduction mechanism of
CH3-(CH2)4-C̩ H-CH=CH-CH=CH-CH2(CH2)7-COOH
metals even at low temperatures. The site of radical formation
O̩ ↓ in saturated fatty acids is different from those of unsaturated
CH3-(CH2)3-CH2 + O=CH-CH=CH-CH=CH-CH2(CH2)7- oleic or linoleic acids. The alkyl radical of saturated fatty
COOH acids is formed at α position of the carboxyl group having
electron-withdrawing property. The high interfacial tension
↓ in the frying system breaks steam bubbles and forms a steam
Termination: CH3-(CH2)3-CH3 blanket over the oil surface. The steam blanket reduces
the contact between the oil and oxygen, and lowers the oil
Figure 2. Thermal oxidation of oil (Min, 2007) oxidation (Blumenthal 1991). Addition of sesame oil can also
improve thermo oxidative stability of the frying oil (Chung,
et al., 2004).

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Figure 4: POV of frying oil at different time and temperatures A, B, C (Nawar, 1984)

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Rancidity have optimal oil absorption. Similarly, over fried foods at

higher temperature and longer frying time than the optimum
Rancidity of oil and fat is attributed to autoxidation. These frying have darkened and hardened surfaces and a greasy
volatile aldehyde compounds have been found to have texture due to the excessive oil absorption (Blumenthal,
certain potential threat to health of consumer (Kamal- 1991). Fat content of instant fried noodles tends to increase
Eldin, 1996, Seppanen, 2002) especially the genotoxic and as flour protein content decreases while fat absorption of
cytotoxic properties of unsaturated aldehydes (Guillén, instant noodles tends to increase as starch amylose content
2008). The genotoxic and cytotoxic olefine aldehyde- decreases (Park and Baik, 2002).The hydrolysis, oxidation,
contained compounds produced have been reported to be and polymerization of oil are common chemical reactions in
absorbed by the fried material (Seppanen, 2004, Seppanen, frying oil and produce volatile or nonvolatile compounds.
2006). Volatile compounds such as acids, hydrocarbons, The nonvolatile compounds in the oil change the physical
alcohols, aldehydes, ketones, esters, lactones and aromatic and chemical properties of oil and fried foods.
compounds were detected in separate studies carried out by
(Kawada, 1967; Yasuda, 1968; Chang, 1978; Thompson, Acrylamide formation
1978; May, 1983.) Rancidity of frying oil and fried noodles
occurs manly due to poor storage technique or long storage Acrylamide refers to 2-propenamide (C3H5NO) which has
time. also been known as, ethylene carboxamide, acrylic acid
amide, vinyl amide or propenoic acid amide. Acrylamide
Formation of polymers appears as a white crystalline solid and the solubilities
(expressed in units of g/100mL of solvent at 300C are: water
The major decomposition products of frying oil are 215.5, methanol 155, ethanol 86.2, acetone 63.1, ethyl acetate
nonvolatile polar compounds and triacylglycerol dimers 12.6, chloroform 2.66, benzene 0.346, and heptanes 0.0068.
and polymers. Dimers and polymers are large molecules The melting point is 84.5°C and its boiling point is 125°C at
with a molecular weight range of 692 to 1600 Daltons and 25 mmHg and 192.6°C at atmospheric pressure (Budavari,
formed by a combination of -C-C-, -C-O-C-, and -C-O- et al., 1989). Acrylamide is formed when food, particularly
O-C- bonds (Stevenson, et. al., 1984; Kim, et. al., 1999). those rich in carbohydrate are cooked at above 1200C
Polymerization in deep-fat frying is radical reactions. Allyl (WHO/FAO, 2002) especially those prepared by grilling,
radicals are formed preferably at methylene carbons α to roasting, baking, frying and deep-frying (Risk Assessment
the double bonds. Dimers are formed from the reactions of Section, 2003). Acrylamide in the instant noodles ranged
allyl radicals by C-C linkage. Polymers formed in deep-fat from 11.6 to 77.4 μg/kg (Kawila, 2011). In Noodles, amino
frying are rich in oxygen (Tseng et al., 1996). Polymers compound and aldoses when mediated by high processing
accelerate further degradation of the oil, increase the oil temperature may react to give melanoidin, end product of
viscosity, reduce the heat transfer, produce foam during Maillard reaction. The Maillard reaction is exceptionally
deep-fat frying, and develop undesirable color in the food. widespread. It occurs virtually ubiquitously in foodstuffs,
Polymers also cause the high oil absorption to foods. The particularly during processing at elevated temperatures
major reaction products formed during deep-fat frying are, (Nursten, 2005). Carbonyl compounds produced in the lipid
oxidized decomposition compounds, hydrolysis products, oxidation pathway competes with carbohydrate-derived
oxidized TAG polymers, cyclic fatty acid monomers, trans carbonyls for amino compounds to produce carbonyl-amine
isomers, TAG polymerized products, sterol derivatives, reaction products (Zamora, 2011)
antioxidant alterations, heterocyclic compounds and
acrylamide (Zhang, 2012). Researchers have found that acrylamide possesses
neurotoxicity, genetic toxicity and carcinogenicity
Frying: Integrated relationship between of lipid, pro- (Friedman, 2003; Hagmar, Wirfält, Paulsson, & Törnqvist,
tein, starch and water 2005; Jafari, 2011; Keramat, 2011). Acrylamide is mainly
produced by the reaction between some amino acids and
Upon addition of the food to the hot oil, the surface reducing sugar on high temperature or during the pathway of
temperature of the food rises rapidly. The water at the maillard reaction (Stadler et al., 2002 ; Jafari, 2011; Keramat,
surface immediately starts boiling and surrounding oil is 2011). Amino acid combined with a dicarbonyl compound
cooled down, compensated by convection. As the boiling and then subjected to intramolecular rearrangement of ion
furthers, the convection is further intensified by the turbulent and strecker degradation to form an acrolein, a hydrogen
water vapor. Due to the evaporation, surface drying will nitride and other compounds or strecker aldehyde (Donald,
occur. The evaporation will also lead to shrinkage and et al., 2002). Acrylamide is formed from the combination of
development of surface porosity and roughness (Mellema, acrylic acid and hydrogen nitride or ammonium originated
2003).Foods fried at the optimum temperature and time have from nitrogen containing compounds.
golden brown color, are properly cooked, and crispy, and

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Except the reducing sugar as source of carbonyl compound, such as TBHQ, tocopherols (vitamin E), BHA, or BHT or
some of the lipid oxidation products which occur during their mixtures TBHQ is reported to have the best efficiency
deep-fat frying, promote or participate in the formation of to prolong the shelf life of fried noodles.
acrylamide. The more susceptible to oxidation of the frying
oil, the more Acrylamide is formed (Capuano, et al., 2010). It was found that Maillard reactions generating compounds
responsible for smashing taste, aroma and color of foods are
Acrylamide concentration is much higher when palmolein is coupled with formation of acrylamide from asparagine and
used as frying oil due to its high content of diglycerides (6 to reducing sugars (Zhang, 2009). Currently it is clearly known
8%). This might be attributed to another formation pathway that this compound is also one of triacylglycerols thermal
of the aminoacid precursor of acrolein (Klostermann, 2002) degradation products that is formed during food processing
or formed from glycerol when the frying temperature at very high temperature. The intensity of acrylamide
is higher than the smoke point of frying oil (Claeys, formation in foods containing asparagine and reducing
2005). Therefore, control of the type of frying oil, frying sugars depends on initial concentrations of these precursors
temperature and time are effective to control the producing and their ratio as well as on temperature and duration of
of acrylamide. Palm oil contains about 45% saturated fat thermal treatment, water activity and pH (Shibamoto, 2005;
and is more stable than palmoleinand other vegetable oils Claus, et al., 2008; Hedegaard, 2008).
(Fennema, 1985). Among myriad of choices of atioxidants,

Aldose N-Substituted Glycosylamine

Amadori Rearrangement

1-Amino-1-Deoxy-2 Ketose Fission Products;

Acetol, butanedione,
2-oxopropanal
Schiff Base of HME of Reductones
Furfural
Aldehvdes
Dehydro Reductones

+ Amino
HMF or Furfural Aldols and N-Free Polymers Compounds

+ Amino Compound + Amino Compound

Melanoidins: brown nitrogenous polymers and compolymers

Figure 5. Reaction pathway of non enzymatic browning in food. Adopted from Nursten, 2005.

Traditionally, High Performance Liquid Chromatography Noodle color and Noodle darkening
(HPLC) and Gas Chromatography (GC) were only used
to measure acrylamide quantitatively. Novel analytical During frying, noodle develops characteristic golden or
technique uses Capillary Ecectrophoresis (CE), Enzyme yellow color. The degree of yellowness in noodles is not
Linked Immunological Assay (ELISA) and Electrochemical only dependent on the alkali used in formulation alone but
biosensors. Among these methods, use of biosensors also on the wheat variety and yellow pigment of the flour
are and ELISA are the most promising one as they allow (Kruger, 1996). Flavones are colorless at acidic pH and
fast screening of numerous samples without usage of present in great amount in germ and bran. Raw noodle
sophisticated apparatus. HPLC, GC and CE are time become darker and yellow with time after manufacture as
consuming sample preparation and use expensive equipment attributed by presence of bran specks ad enzymatic browning
(Oracz, et al., 2011). by polyphenol oxidases (Moss, 1985). Polyphenol oxidases,
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tyrosinase, phenol oxidase, phenolase) exist in multiple References

forms and are concentrated in the bran (Kruger, 1988). The
Bekes F., Zawistowska U. and Bushuk W. (1983). Protein-lipid
enzymes oxidizes phenols to quinones that turns into dark
complexes in the gliadin fraction [Wheat flour, bread-making
colored pigments (melanoidins) by self-polymerization and properties]. Cereal chemistry.
resonance stabilization of aromatic ring structure so formed
or by interaction of amino group with proteins. Microscopic Blumenthal M. M. (1991). A new look at the chemistry and physics
of deep-fat frying. Food technology (USA).
examination of noodle shows that most darkening is in
the form of spots developing around a nucleus of non- Budavari S., O’Neil M. J., Smith A. and Heckelman P. E. (1989).
endosperm material (Moss, 1985). The merck index (Vol. 11): Merck Rahway, NJ.
CAC (2016). Standard for Durum Wheat Semolina and Durum
Microscopic imagining of Noodles Wheat Flour (CODEX STAN 178-1991). In: Codex Alimentarious:
http://www.codexalimentarius.org/standards/list-of-standards/
Most of the increased understanding of the mechanism of en/? provide=standards& orderField=fullReference&sort=asc&
molecular interactions has been brought about by improved num1=CODEX
imaging techniques. Recently, confocal laser scanning Chang S. P. (1978). Chemical-reactions involved in deep-fat frying
microscopy (CLSM) could be regarded as a potential tool of foods. Journal of the American Oil Chemists’ Society 55: 718-
for understanding the effect of processing and the influence 727.
on bio- macromolecules. A microstructural change in instant
Choe E. and Min D. (2007). Chemistry of deep fat frying oils. J. of
noodles during production has been studied using CLSM to Food Science, 72(5): R77-R86.
monitor microstructural differences (Dürrenberger, et al.,
2001; Lalana, 2001; Sangpring, et al., 2015). Chung J., Lee J. and Choe E. (2004). Oxidative stability of soybean
and sesame oil mixture during frying of flour dough. Journal of
Conclusion and recommendations Food Science, 69(7): 574-578.
Claeys W. V. (2005). Quantifying the formation of carcinogens
Beyond art of just producing noodles out of wheat flour, during food processing: acrylamide. Trends in Food Science &
if we become able to understand physiochemical reaction Technology 16: 181–193.
mechanisms along with different stages of processing, it Claus A., Carle R. and Schieber A. (2008). Acrylamide in cereal
beneficial not only from economic prospective of industry products: A review. Journal of cereal Science, 47(2):118-133.
but also from health prospective of consumer. Acrylamide
Cuesta C., Sánchez-Muniz F., Garrido-Polonio C., López-Varela
possesses neurotoxicity, genetic toxicity and carcinogenicity
S. and Arroyo R. (1993). Thermoxidative and hydrolytic changes
(Friedman, 2003). Consumers are concerned of negative in sunflower oil used in fryings with a fast turnover of fresh oil.
impacts of Maillard Reaction Products (MRPs) on health Journal of the American Oil Chemists Society, 70(11): 1069-1073.
and their desirability of reducing fat, salt and gluten
Donald S., Bronislrnv L. and Andrew T. (2002). ACrylarnide is
content of deep fried products obliges food industries to formed in the maillard reaction. Nature, 419: 448-449.
study Several physicochemical changes that take place
during processing (gluten network, gelatinization, Maillard Dürrenberger, M. B., Handschin, S., Conde-Petit, B., & Escher, F.
(2001). Visualization of food structure by confocal laser scanning
reactions, glass transitions). This will lead to discovery
microscopy (CLSM). LWT-Food Science and Technology,
beneficial organoleptic properties and color of the crust. 34(1):11-17.
Therefore, industries should find intelligent way of utilizing
the resources at its optimum. For example: optimum Edoardo Capuanoa T. O. (2010). Lipid oxidation promotes
acrylamide formation in fat rich model system. Food Research
temperature of frying oil, heat supplied, mixing time,
International, 1021-1026.
steaming time and temperature etc. This will help to save
surfeit inputs like steam, heat, frying oil etc. and meet the Fennema R. O. (1985). Food Chemistry, 2nd ed. New York, USA:
quality of the product at its highest degree of safety. Thus Marcel Dekker.
produced Asian Noodles should have protein (7-10%), fat Frega N. M. M. (1999). Effects of free fatty acids on oxidative
(16-22%) and moisture (<3%) (Hou, 2011) stability of vegetable oil. American Oil Chem. Soc., 76:325–9.
Friedman M. (2003). Chemistry, biochemistry and safety of
However, when looked with meticulous scrutiny, there
acrylamide. A review. Journal of Agricultural and Food Chemistry,
are myriad of enigmas. Reaction pathway of acrylamide 4504-4526.
formation is still nebulous and much work to be done on
Fu B. X. (2008). Asian noodles: History, classification, raw
formation of amadori compounds and different polymers
materials, and processing. Food Research International, 888-902.
formed during frying and its precise detection. Researches
can be done to overcome these problems and to have insight Fu B., Assefaw E., Sarkar A. and Carson G. (2006). Evaluation
knowledge to the perfection. of durum wheat fine flour for alkaline noodle processing. Cereal
foods world, 51(4): 178-183.

8
 Shrestha et al.,: J. Food Sci. Technol. Nepal, Vol. 9 (1-10), 2016

Gary G. and Hou R. S. (2013). Relationship Between Lu, Q., Guo, S., & Zhang, S. (2009). Effects of flour free lipids on
Physicochemical Properties of Wheat Flour, Wheat Protein textural and cooking qualities of Chinese noodles. Food Research
Composition, and Textural Properties of Cooked Chinese White International, 42(2): 226-230.
Salted Noodles. Cereal Chemistry, 419-429.
Maher G. (1983). Alkali gelatinization of starches. Starke, pp.
Guillén M. G. (2008). Toxic Oxygenated, unsaturated aldehyde and 35:226 - 234.
their study in foods: a review. Critical Reviews in Food Science and
May W. P. ( 1983). Chemical reactions involved in the deep- fat
Nutrition 48: 119-136.
frying of foods. IX. Identification of the volatile decomposition
Hagmar L., Wirfält E., Paulsson B. and Törnqvist M. (2005). products of triolein. Journal of the American Oil Chemists’ Society
Differences in hemoglobin adduct levels of acrylamide in the 60: 990–995.
general population with respect to dietary intake, smoking
McMaster G. J. (1983). Protein – carbohydrate complexes in gluten:
habits and gender. Mutation Research/Genetic Toxicology and
fractionfractionation and proximate composition (functionality of
Environmental Mutagenesis, 580(1): 157-165.
gluten in bread doughs). Cereal Sci., 77–85.
Hedegaard R.V. and F. H. (2008). Kinetics of formation of
Mellema M. (2003). Mechanism and reduction of fat uptake in deep
acrylamide and Schiff base intermediates from asparagine and
fat fried foods. Trends in Food Science and Technology, 364-373.
glucose. Food Chemistry, 917-925.
migration, and texture of rice noodle. LWT - Food Science and
Hou, G. G. (2011). Asian noodles: Science, technology, and
Technology 64 :1107-1113.
processing: John Wiley & Sons.
Moss H. (1985). Chinese Noodle Production: Wheat Flour Quality
Houhoula D. O. (2003). Effect of procesing time and temperature
and Processing Factors. Australian Wheat Board, Melbourne.
on the accumulation of polar compounds in cottonseed oil during
deep fat frying. Journal of the science of Food and Agriculture, Moss H., Miskelly D. and Moss R. (1986). The effect of alkaline
314-319. conditions on the properties of wheat flour dough and Cantonese-
style noodles. Journal of cereal Science, 4(3): 261-268.
Jafari J. K. (2011). Acrylamide in baking products: a review article.
Food Bioprocess Technology, 530-543. Moss R. G. (1987). The Influence of ingredients and processing
variables on the quality and microstructur of Hokkien, Cantonese
Kamal-Eldin A. A. (1996). Aldehyde acids in frying oil: formation,
and instant noodles. Food Microstructure, (pp. 63 - 74).
toxicological significance and analysis. Grasas y Aceites 47: 342-
348. Nawar W. W. (1969). Thermal degradation of lipids. Journal of
agricultural and food chemistry, 17(1): 18-21.
Karim R. (1990). Texural studies on chinese wet noodles (Hokkien-
style noodles. Universiti pertanian Malaysia. Nawar W. W. (1984). Chemical changes in lipids produced by
thermal processing. Journal of chemical education, 61(4): 299.
Kawada T. K. (1967). Chemical reactions involved in the deep fat
frying of foods. II. Identification of acidic volatile decom-position Naz S., Siddiqi R., Sheikh H. and Sayeed S. A. (2005). Deterioration
products of corn oil. . Journal of the American Oil Chemists’ of olive, corn and soybean oils due to air, light, heat and deep-
Society 44: 131–135. frying. Food Research International, 38(2): 127-134.
Kawila N. (2011). Studies of niacin and acrylamide during Noda T., Tohnooka T., Taya S. and Suda I. (2001). Relationship
processing of instant Asian noodles . Melbourne, Australia. between physicochemical properties of starches and white salted
noodle quality in Japanese wheat flours. Cereal chemistry, 78(4):
Keramat A. L. (2011). Acrylamide in foods: chemistry and analysis.
395-399.
A review. Food Bioprocess Technology, 340-363.
Nursten H. (2005). The Maillard Reaction Chemistry, Biochemistry
Kim, I.-H., Kim, C.-J., & Kim, D.-H. (1999). Physicochemical
properties of methyl linoleate oxidized at various temperatures. and Implications. The University of Reading, Reading, UK: Royal
Korean Journal of Food Science and Technology, 31(3), 600-605. Society of Chemistry.

Klostermann C. G. (2002). Analsis of Acrylamide and mechanisms Ohio N. S. (1985). Influence of Flour Protein, extraction rate,
of its formation in deep fird products. European Journal of Food particle size and starch damage on the quality charactristics of dry
Science and Technoogy, 762-771. noodles. Food Microstructure, pp. 441- 446.

Kruger J. (1996). Asian Noodles. In R. B. James E. Kruger, Pasta Oracz J., Nebesny E. and Żyżelewicz D. (2011). New trends in
and Noodle Technology. quantification of acrylamide in food products. Talanta, 86: 23-34.

Kruger J., Reed G. and Pomeranz Y. (1988). Enzymes and color. Park C. S. and Baik B.-K. (2002). Flour characteristics related to
Wheat: chemistry and technology. Volume I. (Ed. 3): 441-500. optimum water absorption of noodle dough for making white salted
noodles. Cereal chemistry, 79(6): 867-873.
Lalana Chewangkul, O. N. (2001). Microstructural Changes
in Instant Noodles During Production via Triple Staining and Pokorny J. (1981). Browning from lipid-protein interactions.
Confocal Laser Scanning Microscopy andScanning Electron Progress in food and nutrition science.
Microscopy. population with respect to dietary intake, smoking habits and
Lascaray L. (1949). Mechanism of fat splitting. Industrial & gender. Mutation Reesearch: Genetic Toxicity and Environmental
Engineering Chemistry, 41(4): 786-790. Mutagenesis, 157-165.

9
 Shrestha et al.,: J. Food Sci. Technol. Nepal, Vol. 9 (1-10), 2016

Pronyk C. C. (2008). effects of dough resting tme and saturated Tsai C. S. (2007). Biomacromolecules, introduction to structure
steam pre-treatment on the textural properties of superheated function and informatics. New jersey: John Wiley & Sons.
steam processed nstant Asian noodles. Canadian Biosystems
WHO/FAO (2002). Health Implications of Acrylamide in Food:
engineering.
Report of a joint FAO/WHO consultation. Geneva: WHO.
Rho K. S. (1986). Retardatio of rancidity in deep fried instant
Wu T. P. (1998). Modern noodle based foods-product range and
noodles (ramyon). JACOS, pp. 63: 251 - 256.
production methods. Pacific people and their foods, 37- 89.
Risk Assessment Section. (2003). Acrylamide in Food. Food and
Yasuda K. R. (1968). Chemical reactions involved in the deep fat
Public Health Branch of the Food and Environmental Hygiene
frying of foods. IV. Identification of acidic volatile decomposition
Department of the Government of the Hong Kong Special
products of hydrogenated cottonseed oil. . Journal of the American
Administrative Region.
Oil Chemists’ Society 45: 625–628.
Rombouts I., Jansens K. J., Lagrain B., Delcour J. A. and Zhu K.-
Zamora R. H. (2011). The maillard reaction and lipid oxidation. .
X. (2014). The impact of salt and alkali on gluten polymerization
Lipid Technology 23: 59–62.
and quality of fresh wheat noodles. Journal of cereal Science,
60(3):507-513. Zawistowska U. B. (1985). Gluten proteins with high affinity to
flour lipids. Cereal Chem, 62: 284–289.
Romero A., Cuesta C. and Sánchez-Muniz F. J. (1998). Effect of oil
replenishment during deep-fat frying of frozen foods in sunflower Zhang Q., Saleh A. S., Chen J. and Shen Q. (2012). Chemical
oil and high-oleic acid sunflower oil. Journal of the American Oil alterations taken place during deep-fat frying based on certain
Chemists' Society, 75(2): 161-167. reaction products: a review. Chemistry and physics of lipids,
Ross A. S. (2005). Guidelines for the laboratory manuacture of 165(6): 662-681.
asian wheat flour noodles. Cereal Foods World 50: 296-304.
Zhang Y., Ren Y. and Zhang Y. (2009). New research developments
Sangpring, Y., Fukuoka, M., & Ratanasumawong, S. (2015). The on acrylamide: analytical chemistry, formation mechanism, and
effect of sodium chloride on microstructure, water migration,
mitigation recipes. Chemical reviews, 109(9): 4375-4397.
and texture of rice noodle. LWT-Food Science and Technology,
64(2):1107-1113.
Seppanen C. A. (2006). The effect of intermittent and
continuous heating of soybean oil at frying temperature on the
formation of 4-hydroxy- 2-trans-nonenal and other unsaturated
hydroxyaldehydes. Journal of Ameerican Oil Chemists' Society
83: 121-127.
Seppanen C. C. (2002). Formation of 4-hydroxynonenal, atoxic
aldehyde in soybean oil at frying temperature. Journal of American
Oil Chemists' Society 79: 1033-1038.
Seppanen C. C. (2004). Incorporation of toxic aldehyde 4-hydroxy-
2-trans-nonenal into food fried in thermally oxidized soybean oil.
Journal of american Oil Chemists' Society 81: 1137-1141.
Shibamoto S. E. (2005). Correlation of Acrylamide Generation in
Thermally Processed Model Systems of Asparagine and Glucose
with Color Formation, Amounts of Pyrazines Formed, and
Antioxidative Properties of Extracts. Journal of Agriculture and
Food Chemistry, 4813–4819.
Stadler R. H., Blank I., Varga N., Robert F., Hau J., Guy P. A. and
Riediker S. (2002). Food chemistry: acrylamide from Maillard
reaction products. Nature, 419(6906): 449-450.
Stevenson, S., Vaisey-Genser, M., & Eskin, N. (1984). Quality
control in the use of deep frying oils. Journal of the American Oil
Chemists Society, 61(6), 1102-1108.
Terada M. M. (1978). A component of wheat flour globulin
polymerized at alkaline sides and depolymerized by reduction
revesrsibility. In Agricultural and Biological Chemistry (pp.
42:1397-1402).
Thompson J. M. (1978). Chemical reactions involved in the deep-
fat frying of foods. VII. Identification of volatile decompo-sition
products of trilinolein. Journal of the American Oil Chemists’
Society 55: 897–901.

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