Bone Grafts in Dentistry
Bone Grafts in Dentistry
Bone Grafts in Dentistry
J Pharm Bioallied Sci. 2013 Jun; 5(Suppl 1): S125–S127. PMCID: PMC3722694
doi: 10.4103/0975-7406.113312: 10.4103/0975-7406.113312 PMID: 23946565
Department of Oral and Maxillofacial Surgery, Bhabha College of Dental Sciences, Bhopal, Madhya
Pradesh, India
1Department of Prosthodontics, Vydehi Institute of Dental Sciences and Research Centre,
India
Address for correspondence: Dr. Prasanna Kumar, E-mail: [email protected]
This is an open-access article distributed under the terms of the Creative Commons Attribution-
Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly cited.
Abstract
Bone grafts are used as a filler and scaffold to facilitate bone formation and promote wound
healing. These grafts are bioresorbable and have no antigen-antibody reaction. These bone
grafts act as a mineral reservoir which induces new bone formation.
Keywords: Allograft, autograft, bone reconstruction, bone repair, calcium sulphate, ceramic,
hydroxyapatite, implant, polymer
Bone grafting is a surgical procedure that replaces missing bone with material from patient′s
own body, an artificial, synthetic, or natural substitute. Bone grafting is possible because
bone tissue has the ability to regenerate completely if provided the space into which it has to
grow. As natural bone grows, it generally replaces the graft material completely, resulting in
a fully integrated region of new bone.
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a. Allograft-based bone graft involves allograft bone, used alone or in combination with
other materials (e.g., Grafton, OrthoBlast).
b. Factor-based bone graft are natural and recombinant growth factors, used alone or in
combination with other materials such as transforming growth factor-beta (TGF-beta),
platelet-derived growth factor (PDGF), fibroblast growth factors (FGF), and bone
morphogeneic protein (BMP).
c. Cell-based bone grafts use cells to generate new tissue alone or are added onto a
support matrix, for example, mesenchymal stem cells.
d. Ceramic-based bone graft substitutes include calcium phosphate, calcium sulphate, and
bioglass used alone or in combination; for example, OsteoGraf, ProOsteon, OsteoSet.
The biologic mechanisms that provide a rationale for bone grafting are osteoconduction,
osteoinduction, and osteogenesis.[2]
Osteoconduction
Occurs when bone graft material serves as a scaffold for new bone growth, which is
perpetuated by the native bone. Osteoblasts from the margin of defect that is being grafted,
utilize the bone graft material as a framework upon which to spread and generate new bone.
[1] In the very least, a bone graft material should be osteoconductive.
Osteoinduction
Involves stimulation of osteoprogenitor cells to differentiate into osteoblasts and then begins
formation of new bone. The most widely studied type of osteoinductive cell mediators are
BMPs.[2] A bone graft material that is osteoconductive and osteoinductive will not only
serve as a scaffold for currently existing osteoblasts but will also trigger formation of new
osteoblasts, promoting faster integration of the graft.
Osteopromotion
Osteogenesis
It occurs when vital osteoblasts originating from bone graft material contributes to the
growth of new bone along with bone formation.
Autograft
Autologous or autogenous bone grafting involves utilizing bone obtained from same
individual receiving the graft. Bone can be harvested from nonessential bones, such as from
iliac crest, mandibular symphysis (chin area), and anterior mandibular ramus (coronoid
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process). When a block graft will be performed, autogeneous bone is the most preferred
because there is less risk of graft rejection as the graft is originated from the patient′s body.
[3] It would be osteoinductive and osteogenic, as well as osteoconductive. Disadvantage of
autologous grafts is that additional surgical site is required, another potential location for
postoperative pain and complications.[3]
All bones require blood supply in the transplanted site. Depending on where the transplant
site is and size of the graft, an additional blood supply may be required. For these types of
grafts, extraction of the part of the periosteum and accompanying blood vessels along with
the donor bone is required. This kind of graft is known as a free flap graft.
Allografts
Allograft is derived from humans. The difference is that allograft is harvested from an
individual other than the one receiving the graft. Allograft bone is taken from cadavers that
have donated their bone so that it can be used for living people who are in need of it; it is
typically sourced from a bone bank.
2. FDBA
3. DFDBA
The use of allografts for bone repair often requires sterilization and deactivation of proteins
normally found in healthy bone. Contained in the extracellular matrix of bone tissue are the
full cocktail of bone growth factors, proteins, and other bioactive materials necessary for
osteoinduction and successful bone healing; the desired factors and proteins are removed
from the mineralized tissue by using a demineralizing agent such as hydrochloric acid. The
mineral content of the bone is degraded, and the osteoinductive agents remain in a
demineralized bone matrix (DBM).
Synthetic variants
Artificial bone can be created from ceramics such as calcium phosphates (e.g., HA and
tricalcium phosphate), bioglass, and calcium sulphate are biologically active depending on
solubility in physiological environment.[5] These materials combine with growth factors,
ions such as strontium or mixed with bone marrow aspirate to increase biological activity.
The presence of elements such as strontium can result in higher bone mineral density (BMD)
and enhanced osteoblast proliferation.
Xenograft
Xenogratfs are bone grafts from a species other than human, such as bovine and are used as a
calcified matrix.
Alloplastic grafts
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Alloplastic grafts may be made from hydroxyapatite, a naturally occurring mineral (main
mineral component of bone), made from bioactive glass. Hydroxyapatite is a synthetic bone
graft, which is the most used now due to its osteoconduction, hardness, and acceptability by
bone. Some synthetic bone grafts are made of calcium carbonate, which start to decrease in
usage because it is completely resorbable in short time and makes breaking of the bone
easier. Finally used is the tricalcium phosphate in combination with hydroxyapatite and thus
giving effect of both, osteoconduction and resorbability.
Growth factors
Growth factors enhanced grafts are produced using recombinant DNA technology. They
consist of either human growth factors or morphogens (BMPs in conjunction with a carrier
medium, such as collagen).
The factors and proteins that exist in bone are responsible for regulating cellular activity.
Growth factors bind to receptors on cell surfaces and stimulate intracellular environment to
act. Generally, this activity translates to a protein kinase that induces a series of events
resulting in transcription of messenger ribonucleic acid (mRNA) and ultimately into the
formation of a protein to be used intracellularly or extracellularly. The combination and
simultaneous activity of many factors results in controlled production and resorption of bone.
These factors, residing in extracellular matrix of bone, include TGF-beta, insulin like growth
factors I and II, PDGF, FGF, and BMPs.[6,7] Cell-based bone graft substitutes: Stem cells
are cultured in the presence of various additives such as dexamethasone, ascorbic acid, and
β-glycerophosphate to direct the undifferentiated cell towards osteoblast lineage.
The addition of TGF-beta and BMP-2, BMP-4, and BMP-7 to the culture media can also
influence the stem cells towards osteogenic lineage. Mesenchymal stem cells have also been
seeded onto bioactive ceramics conditioned to induce differentiation to osteoblasts.
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surrounding bone. Different types of calcium phosphates are tricalcium phosphate, synthetic
hydroxyapatite, and coralline hydroxyapatite; available in pastes, putties, solid matrices, and
granules.
Such calcium phosphates products include Bio-Oss and OsteoGraft. Both products use
hydroxyapatite, either as a particulate (Bio-Oss) or as blocks and particulates (OsteoGraft).
Pro-Osteon is a unique product based on sea coral, which is converted from calcium
carbonate to calcium hydroxyapatite. The advantage of this material is that the structure of
coral, which is similar to that of trabecular bone.
Degradable synthetic polymers, like natural polymers are resorbed by the body. The benefit
of having the implant resorbed by the body is that, the body is able to heal itself completely
without remaining foreign bodies.
Uses
The most common use of bone grafting is in application of dental implants, in order to
restore edentulous area of a missing tooth. In general, bone grafts are either used in block
(such as from chin or ascending ramus area of lower jaw) or particulated, in order to be able
to adapt it better to a defect. The grafted, vascularized fibulas have been used to restore
skeletal integrity to long bones of limbs in which congenital bone defects exist and to replace
segments of bone after trauma or malignant tumor invasion. The periosteum and nutrient
artery are generally removed with piece of bone so that the graft will remain alive and grow
when transplanted into new host site. Once the transplanted bone is secured into its new
location, it generally restores blood supply to the bone on which it has been attached.
Besides the main use of bone grafting in dental implants, this procedure is used to fuse joints
to prevent movement, repair broken bones that have bone loss, and repair broken bone that
has not yet healed.[9]
Footnotes
Source of Support: Nil.
References
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Mortal Wkly Rep. 2001;50:1081–3. [PubMed: 11770503]
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infections: United States, 2002. MMWR Morb Mortal Wkly Rep. 2002;51:207–10.
[PubMed: 11922189]
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[PubMed: 18725869]
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