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    Density Gradient Centrifugation

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    Matthew Meselson and Franklin Stahl were interested in researching methods to prove or disprove the Watson-Crick model of DNA structure. They developed the technique of density gradient centrifugation to separate DNA strands based on their density. Through their famous 1958 experiment, they demonstrated that DNA replication is semi-conservative, with each parental DNA strand serving as a template to produce two daughter double helices containing one old and one new strand. Their elegant experiment provided the first clear evidence supporting the Watson-Crick model and was revolutionary for molecular biology.

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    Density Gradient Centrifugation

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    api-513510848
    144 views18 pages
    Matthew Meselson and Franklin Stahl were interested in researching methods to prove or disprove the Watson-Crick model of DNA structure. They developed the technique of density gradient centrifugation to separate DNA strands based on their density. Through their famous 1958 experiment, they demonstrated that DNA replication is semi-conservative, with each parental DNA strand serving as a template to produce two daughter double helices containing one old and one new strand. Their elegant experiment provided the first clear evidence supporting the Watson-Crick model and was revolutionary for molecular biology.

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    density gradient centrifugation

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    Matthew Meselson and Franklin Stahl were interested in researching methods to prove or disprove the Watson-Crick model of DNA structure. They developed the technique of density gradient centrifugation to separate DNA strands based on their density. Through their famous 1958 experiment, they demonstrated that DNA replication is semi-conservative, with each parental DNA strand serving as a template to produce two daughter double helices containing one old and one new strand. Their elegant experiment provided the first clear evidence supporting the Watson-Crick model and was revolutionary for molecular biology.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    144 views18 pages

    Density Gradient Centrifugation

    Uploaded by

    api-513510848
    Matthew Meselson and Franklin Stahl were interested in researching methods to prove or disprove the Watson-Crick model of DNA structure. They developed the technique of density gradient centrifugation to separate DNA strands based on their density. Through their famous 1958 experiment, they demonstrated that DNA replication is semi-conservative, with each parental DNA strand serving as a template to produce two daughter double helices containing one old and one new strand. Their elegant experiment provided the first clear evidence supporting the Watson-Crick model and was revolutionary for molecular biology.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    of 18

    Density Gradient

    Centrifugation
    Discovery by: Matthew Meselson and Franklin Stahl
    Presentation by: Sarah Gorbatov and Nurit Schlosberg
    Matthew Meselson: Background Check
    ❏ Born in Denver on May 24, 1930
    ❏ Graduated high school 1.5 years
    early = nerd (he never received a
    diploma!)
    ❏ Enrolled at UChicago and studied
    liberal arts
    ❏ Travelled throughout Europe
    ❏ Attended Berkeley and UChicago,
    studying chemistry
    ❏ Began working under Pauling at
    CalTech and researching
    equilibrium density gradient
    centrifugation/x-ray crystallography
    Stahl: Background Check
    ❏ Born on October 8,
    1929
    ❏ Attended public
    school in the
    suburbs surrounding
    Boston
    ❏ Also a nerd, surprise
    surprise
    ❏ Studied biology at
    the University of
    Rochester and
    thereafter Harvard
    ❏ Received his PhD for
    the work he did on
    bacterial viruses
    known as phages
    The both of them were interested in
    researching different methods of
    proving or disproving the
    Watson-Crick Rosalind Franklin
    model of double-helical DNA.
    The wedding’s
    tomorrow.

    Meselson’s vision of new experimentation


    methods led the way and Stahl’s expertise
    in mathematics and biology ensured
    success in their expedition!
    Equilibrium density gradient centrifugation
    Semiconservative vs. conservative vs. dispersive
    DNA replication
    “The most beautiful experiment in biology”
    A Deeper Dive Into Centrifugation
    Centrifugal Force
    Cesium salt for the win!
    “The Replication of DNA in Escherichia Coli”
    The End

    CREDITS: This presentation template was created by Slidesgo, including icons by


    Flaticon, infographics & images by Freepik and illustrations by Stories
    Bibliography
    ● http://www.dnaftb.org/20/bio.html#:~:text=M
    atthew%20Meselson%20and%20Franklin%20
    Stahl,enzymes%20that%20can%20replicate%
    20DNA
    ● https://www.nature.com/scitable/topicpa
    ge/semi-conservative-dna-replication-mes
    elson-and-stahl-421/#:~:text=According%2
    0to%20the%20semiconservative%20replica
    tion,synthesized%20double%20helix%20is
    %20a
    ● https://www.khanacademy.org/science/b
    iology/dna-as-the-genetic-material/dna-r
    eplication/a/mode-of-dna-replication-me
    selson-stahl-experiment#:~:text=The%20e
    xperiment%20done%20by%20Meselson,of
    %20a%20new%2C%20complementary%20s
    trand.&text=Some%20of%20your%20cells
    %20are,DNA%20semi%2Dconservatively%2
    0right%20now
    ● https://www.akadeum.com/blog/density-
    gradient-centrifugation/
    ● https://pubs.acs.org/doi/abs/10.1021/ja01
    148a508

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