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    This document is a medical test report for a 22-year-old female named Mrs. G.VARA LAXMI. It indicates that a nasal and throat swab sample collected on April 6, 2021 and received on April 7, 2021 tested negative for SARS-CoV-2 (COVID-19) using real-time PCR. The report provides additional information on Ct values and test limitations.

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    This document is a medical test report for a 22-year-old female named Mrs. G.VARA LAXMI. It indicates that a nasal and throat swab sample collected on April 6, 2021 and received on April 7, 2021 tested negative for SARS-CoV-2 (COVID-19) using real-time PCR. The report provides additional information on Ct values and test limitations.

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    This document is a medical test report for a 22-year-old female named Mrs. G.VARA LAXMI. It indicates that a nasal and throat swab sample collected on April 6, 2021 and received on April 7, 2021 tested negative for SARS-CoV-2 (COVID-19) using real-time PCR. The report provides additional information on Ct values and test limitations.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    53 views1 page

    PDF Text

    Uploaded by

    Frien Kgt
    This document is a medical test report for a 22-year-old female named Mrs. G.VARA LAXMI. It indicates that a nasal and throat swab sample collected on April 6, 2021 and received on April 7, 2021 tested negative for SARS-CoV-2 (COVID-19) using real-time PCR. The report provides additional information on Ct values and test limitations.

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    of 1

    Name : Mrs. G.

    VARA LAXMI Sample ID : 21128767


    Age/Gender : 22 Years/Female Reg. No : 0012104070275
    Referred by : Dr. SELF SPP Code : SPL-CV-647
    Referring Customer : SAI ADITYA DIAGNOSTIC CENTRE Collected On : 06-Apr-2021 10:00 AM
    ITDOSE INFOSYSTEMS PVT. LTD.

    Primary Sample : Nasal and Throat Swabs Received On : 07-Apr-2021 04:07 AM


    Sample Tested In : Viral Transport Medium Reported On : 07-Apr-2021 08:18 AM
    Client Address : Report Status : Final Report

    MOLECULAR BIOLOGY
    Test Name Results Units Ref. Range Method

    SARS-CoV-2 (COVID-19) RT PCR


    SARS-CoV-2 (COVID-19) Negative NA Real Time PCR

    ICMR Registration number for Covid -19 is: SAPALAPVLHT


    Ct Value Result
    Negative Negative results indicate the absence of SARS -CoV-2 in the specimen
    Cts<=24 Strong positive reactions indicative of abundant target Viral RNA
    Cts of 25 to
    Positive reactions indicative of moderate amounts of Viral RNA
    31
    Weak reactions indicative of minimal amounts of target nucleic acid
    Cts >=31
    (Viral RNA) which could present an infection state
    What is the threshold cycle or Ct value?:In a real time PCR assay a positive reaction is detected by accumulation of a fluorescent signal. The Ct (cycle
    threshold) is defined as the number of cycles required for the fluorescent signal to cross the threshold (ie exceeds background level). Ct levels are inversely
    proportional to the amount of target nucleic acid in the sample (ie the lower the Ct level the greater the amount of target nucleic acid in the sample).

    Ct Values differ from kit to kit, lab to lab, Collection Process and transportaion conditions and other factors.
    1. COVID-19 detection requires identifying 3 genes; however 2 genes sepcific to SARS-CoV 2 (COVID-19) are sufficient for a positive detection.
    2. E-gene gene is for the detection of Sarbecovirus (Genus B-betacoronavirus) as a first line test. RdRp gene or N-gene are for detection of severe acute respiratory
    syndrome coronavirus 2 (SARS-CoV-2) and are the confirmatory tests.
    Limitations of the Test: Performance of 2019-nCoV Real-Time RT-PCR Diagnostic Panel has only been established in upper and lower respiratory specimens
    (nasopharyngeal or oropharyngeal swabs, sputum, lower respiratory tract aspirates, bronchoalveolar lavage, and nasopharyngeal wash/aspirate or nasal aspirate).

    Correlate Clinically.

    *** End Of Report ***

    Page 1 of 1

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