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Sabouraud Cycloheximide Chloramphenicol Agar: Intended Use

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57 views

Sabouraud Cycloheximide Chloramphenicol Agar: Intended Use

Uploaded by

nadyasantika
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
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Sabouraud Cycloheximide Chloramphenicol Agar M664

Intended Use:
Recommended for selective isolation and cultivation of pathogenic fungi.
Composition**
Ingredients Gms / Litre
Peptone 10.000
Dextrose (Glucose) 20.000
Chloramphenicol 50mg
Cycloheximide 500mg
Agar 15.000
Final pH ( at 25°C) 6.8±0.2
**Formula adjusted, standardized to suit performance parameters

Directions
Suspend 45.54 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize
by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates.
Caution : Cycloheximide is very toxic. Avoid skin contact or aerosol formation and inhalation.
Some pathogenic fungi may produce infective spores, which are easily dispersed in air, so examination should be carried
out in safety cabinet.

Principle And Interpretation


Sabouraud Dextrose Agar was originally formulated by Sabouraud (7) and further modified by Emmons (3) by
reducing dextrose content and adjusting the pH close to neutral.
Peptone is the source of nitrogenous growth factors while dextrose provides an energy source for the growth
of microorganisms. The media can be rendered selective for fungi by antibiotics such as Chloramphenicol
(1) and Cycloheximide (6), which inhibit some bacteria as well as some saprophytic and pathogenic fungi. This
medium inhibits fungi like Cryptococcus neoformans, Aspergillus, Nocardia, certain Candida species but allow the
dermatophytes to grow well.
Type of specimen
Clinical: Skin infections

Specimen Collection and Handling


For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5).
After use, contaminated materials must be sterilized by autoclaving before discarding.

Warning and Precautions


In Vitro diagnostic use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye
protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard
precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be
referred in individual safety data sheets.

Limitations
1.Some pathogenic fungi may produce infective spores, which are easily dispersed in air, so examination should be carried
out in safety cabinet.
2.Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.

Performance and Evaluation


Performance of the medium is expected when used as per the direction on the label within the expiry period when
stored at recommended temperature.
Please refer disclaimer Overleaf.
HiMedia Laboratories Technical Data

Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of Prepared Medium
Light amber coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 4.5% w/v aqueous solution at 25°C. pH : 6.8±0.2
pH
6.60-7.00
Cultural Response
Cultural characteristics observed after an incubation at 25-30°C for 2-3 weeks.
Organism Inoculum Growth Recovery
(CFU)
*Aspergillus brasiliensis 50-100 none-poor
ATCC 16404 (00053*)
Candida albicans ATCC 50-100 poor-fair <=20%
10231 (00054*)
Escherichia coli ATCC >=104 inhibited 0%
25922 (00013*)
Saccharomyces cerevisiae 50-100 none-poor <=20%
ATCC 9763 (00058*)
Trichophyton 50-100 luxuriant
mentagrophytes ATCC 9533
Trichophyton rubrum ATCC 50-100 luxuriant
28191
Key : (*) - Corresponding WDCM numbers.
(#) - Formerly known as Aspergillus niger

Storage and Shelf Life


Store between dehydrated and the prepared medium at 2-8°C. Use before expiry date on the label. On opening,
product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to
the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry
ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use.
Product performance is best if used within stated expiry period.

Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product.
Follow established laboratory procedures in disposing of infectious materials and material that comes into contact
with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques ().
Reference
1. Ajello L., 1957, J. Chron. Dis., 5:545.
2. Diagnostic Procedures, 1963, 4th ed., APHA
3. Emmons C., Binford C., Uty J. and Kwon-Chung, 1970, Medical Mycology, 2nd ed., Philadelphia: Lea and Febiger.
4.. Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition
5. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)
Manual of Clinical Microbiology, 11th Edition. Vol. 1.
6. MacFaddin J. F., 1985, Media For Isolation-Cultivation Identification - Maintenance of Medical Bacteria, Vol. 1, Williams
and Wilkins, Baltimore.
7. Sabouraud R., 1892, Ann. Dermatol. Syphilol., 3:1061.

Revision :01/ 2020

Please refer disclaimer Overleaf.


HiMedia Laboratories Technical Data

In vitro diagnostic medical


IVD device

CE Marking

8°C Storage temperature

2°C

Do not use if package is


damaged

HiMedia Laboratories Pvt. Limited,


23 Vadhani Industrial Estate,
LBS Marg,Mumbai-86,MS,India

CE Partner 4U ,Esdoornlaan 13, 3951


EC REP
DB Maarn The Netherlands,
www.cepartner 4u.eu

Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6116 9797 Corporate
office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:
[email protected] Website: www.himedialabs.com

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