Himedia M933

Orange Serum Agar M933
Intended use
Recommended for cultivation and enumeration of microorganisms associated with the spoilage of citrus products,
cultivation of Lactobacilli, other aciduric organisms and pathogenic fungi from food and clinical samples.
Composition**
Ingredients Gms / Litre
Tryptone 10.000
Yeast extract 3.000
Dextrose (Glucose) 4.000
Dipotassium hydrogen phosphate 2.500
Orange serum (Solids from 200 ml) 9.000
Agar 17.000
Final pH ( at 25°C) 5.5±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 45.5 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by
autoclaving at 15 lbs pressure (121°C) for 15 minutes. AVOID OVERHEATING. Cool to 45-50°C.Mix well and pour into
sterile Petri plates.
Principle And Interpretation

Fruit juices are generally acidic, with pH values ranging from approximately 2.4 for lemon juice, to 4.2 for tomato juice. The
low pH of these foods is selective for yeast, moulds and a few groups of aciduric bacteria. The microorganisms of greatest
significance in citrus juices are the lactic acid bacteria, primarily species of Lactobacillus and Leuconostoc, yeast and
moulds. Microbial spoilage of these citrus fruit juices are most commonly due to aciduric microbes such as lactic acid
bacteria and yeast. The lactic acid bacteria include Lactobacillus fermentum, L.plantarum, and Leuconostoc mesenteroides.
Orange Serum Agar is recommended by APHA (7) for cultivation of Lactobacilli and other aciduric organisms. Orange Serum
Agar was originally developed by Murdock et al (6) and Hays (1) for examining citrus concentrates. Hays and Reister further
used this medium for studying the spoilage of orange juice (2). Dehydrated agar medium containing orange serum was
reported by Stevens (8). Orange Serum Broth is used to initiate growth of saprophytic, pathogenic fungi in small samples (5).
Tryptone provides essential nitrogenous, carbonaceous compounds, long chain amino acidsand other essential nutrients.
Dextrose (Glucose) serves as the fermentable carbohydrate and energy source. Yeast extract supplies B- complex vitamins,
which stimulate growth. Orange serum provides an optimal environment for the recovery of acid tolerant microorganisms
from citrus fruit products.
Type of specimen
Clinical samples- Skin, Food samples
Specimen Collection and Handling:

For clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4).
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (7).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions

In Vitro diagnostic Use. Read the label before opening the container. Wear protective gloves/protective clothing/eye
protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard
precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be
referred in individual safety data sheets.
Please refer disclaimer Overleaf.

HiMedia Laboratories Technical Data
Limitations :
1. Some strains may show poor growth due to nutritional variations.
Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at
recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.7% agar gel.
Colour and Clarity of prepared medium
Medium to dark amber coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 4.55% w/v aqueous solution at 25°C. pH : 5.5±0.2
pH
5.30-5.70
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 40-48 hours.(Fungal species are incubated at 25-30°C )
Organism Inoculum Growth Recovery

(CFU)
#Aspergillus brasiliensis 50-100 good-luxuriant

ATCC 16404 (00053*)
Candida albicans ATCC 50-100 good-luxuriant >=50%
10231 (00054*)
Lactobacillus acidophilus 50-100 good-luxuriant >=50%
ATCC 4356
Lactobacillus fermentum 50-100 good-luxuriant >=50%
ATCC 9338
Leuconostoc mesentoroides 50-100 good-luxuriant >=50%
ATCC 12291
Saccharomyces cerevisiae 50-100 good-luxuriant >=50%
ATCC 9763 (00058*)
Key : # - Formerly known as Aspergillus niger * - Corresponding WDCM numbers.
Storage and Shelf Life

Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on
the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump
formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation.
Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly
after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product.
Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with
clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
Reference
1.Hays G. L., 1951, Proc. Florida State Hortic. Soc., 54:135.
2. Hays G. L. and Reister D. W., 1952, Food Technol., 6:186.
3.Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
4. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)
Manual of Clinical Microbiology, 11th Edition. Vol. 1.
Please refer disclaimer Overleaf.

HiMedia Laboratories Technical Data
5. MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams
and Wilkins, Baltimore
6. Murdock P. I., Folinazzo J. F., and Troy V. S., 1951, Food Technol., 6:181.
7. Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination of
Foods, 5th Ed., American Public Health Association, Washington, D.C.
8. Stevens J. W., 1954, Food Technol., 8:88.
Revision : 03 / 2020
In vitro diagnostic medical

IVD device
CE Marking
30°C Storage temperature
10°C
Do not use if package is

damaged
HiMedia Laboratories Pvt. Limited,

23 Vadhani Industrial Estate,
LBS Marg,Mumbai-86,MS,India
CE Partner 4U ,Esdoornlaan 13, 3951

EC REP
DB Maarn The Netherlands,
www.cepartner 4u.eu
Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6116 9797 Corporate
office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:
[email protected] Website: www.himedialabs.com

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Orange Serum Agar M933

Principle And Interpretation

Specimen Collection and Handling:

Warning and Precautions

Please refer disclaimer Overleaf.

Performance and Evaluation

Organism Inoculum Growth Recovery

#Aspergillus brasiliensis 50-100 good-luxuriant

Key : # - Formerly known as Aspergillus niger * - Corresponding WDCM numbers.

Storage and Shelf Life

Please refer disclaimer Overleaf.

In vitro diagnostic medical

30°C Storage temperature

Do not use if package is

HiMedia Laboratories Pvt. Limited,

CE Partner 4U ,Esdoornlaan 13, 3951

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