Cambridge IGCSE™

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BIOLOGY 0610/52
Paper 5 Practical Test May/June 2021

1 hour 15 minutes

You must answer on the question paper.

You will need: The materials and apparatus listed in the confidential instructions

INSTRUCTIONS
● Answer all questions.
● Use a black or dark blue pen. You may use an HB pencil for any diagrams or graphs.
● Write your name, centre number and candidate number in the boxes at the top of the page.
● Write your answer to each question in the space provided.
● Do not use an erasable pen or correction fluid.
● Do not write on any bar codes.
● You may use a calculator.
● You should show all your working and use appropriate units.

INFORMATION
● The total mark for this paper is 40.
● The number of marks for each question or part question is shown in brackets [ ].

For Examiner’s Use

Total

This document has 12 pages. Any blank pages are indicated.

DC (ST/JG) 201927/3
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1 Catalase is an enzyme found in living cells. It catalyses the break-down of hydrogen peroxide to
form water and oxygen.

You are going to investigate the effect of catalase concentration on the rate of oxygen production.
You have been provided with a celery extract which contains catalase.

Read all the instructions but DO NOT CARRY THEM OUT until you have drawn a table for
your results in the space provided in 1(a)(ii).

You should use the safety equipment provided while you are carrying out the practical work.

Step 1 Label five test-tubes A, B, C, D and E. Put the test-tubes into the test-tube rack.

Step 2 Use a measuring cylinder to pour 10 cm3 of celery extract into test-tube A.

Step 3 Use the measuring cylinder to transfer 5 cm3 of celery extract from test-tube A to test-
tube B.

Use the measuring cylinder to add 5 cm3 of distilled water to test-tube B. Place a stopper
in test-tube B and shake it three times.

Step 4 Remove the stopper. Use the measuring cylinder to transfer 5 cm3 of the liquid in test-
tube B to test-tube C.

Use the measuring cylinder to add 5 cm3 of distilled water to test-tube C. Place a stopper
in test-tube C and shake it three times.

Step 5 Remove the stopper. Use the measuring cylinder to transfer 5 cm3 of the liquid in test-
tube C to test-tube D.

Use the measuring cylinder to add 5 cm3 of distilled water to test-tube D. Place a stopper
in test-tube D and shake it three times.

Step 6 Remove the stopper. Use the measuring cylinder to transfer 5 cm3 of the liquid in test-
tube D to test-tube E.

Use the measuring cylinder to add 5 cm3 of distilled water to test-tube E. Place a stopper
in test-tube E and shake it three times. Remove the stopper.

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(a) (i) Table 1.1 shows the concentration of the celery extract in test-tubes A to E.

Table 1.1

percentage
test-tube concentration of
celery extract

A 100.00

B 50.00

C
..................

D 12.50

E 6.25

Complete Table 1.1 by calculating and writing in the percentage concentration of celery
extract in test-tube C. [1]

Step 7 Pour a small amount of the celery extract from test-tube A onto the watch glass, as
shown in Fig. 1.1.

watch glass

celery extract

Fig. 1.1

Step 8 Use the forceps to place one of the paper discs into the celery extract in the watch glass.

Step 9 Use the ruler to measure 2 cm from the top (open end) of the remaining unlabelled
test-tube. Mark this distance by drawing a line on the test-tube with the marker pen.

Step 10 Use the forceps to remove the paper disc from the watch glass. Place the paper disc into
the test-tube you prepared in step 9. Push the paper disc to the bottom of the test-tube
with the glass rod.

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Step 11 Carefully pour hydrogen peroxide solution into the test-tube until it reaches the line
you marked on the test-tube in step 9. Immediately start the stop-clock and observe the
paper disc rising.

Stop the stop-clock when the paper disc reaches the surface of the hydrogen peroxide
solution.

Record the time taken for the disc to rise, in your table in 1(a)(ii). Record the time in
seconds. If the paper disc has not reached the surface of the hydrogen peroxide solution
after three minutes, stop the stop-clock and record the time as >180 in your table.

Step 12 Pour the hydrogen peroxide solution and paper disc into the beaker labelled waste.

Empty the contents of the watch glass into the waste beaker. Wipe the watch glass with
a paper towel.

Step 13 Repeat steps 7 to 12 using the celery extract in test-tubes B, C, D and E.

(ii) Prepare a table to record your results.

[4]

(iii) State a conclusion for this investigation.

...........................................................................................................................................

...........................................................................................................................................

..................................................................................................................................... [1]

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(iv) Identify the variable that you have changed (independent variable) and the variable you
have measured (dependent variable) in this investigation.

independent variable .........................................................................................................

...........................................................................................................................................

dependent variable ............................................................................................................

...........................................................................................................................................
[2]

(b) (i) The oxygen gas produced by the reaction forms bubbles on the paper disc. The bubbles
cause the disc to rise to the top of the hydrogen peroxide solution. The time taken for the
disc to rise can be used to calculate the rate of the reaction.

Explain how you could calculate the rate at which the disc rises.

...........................................................................................................................................

...........................................................................................................................................

...........................................................................................................................................

..................................................................................................................................... [2]

(ii) Identify one source of error in step 4 and one source of error in step 11.

error in step 4 ....................................................................................................................

...........................................................................................................................................

...........................................................................................................................................

error in step 11 ..................................................................................................................

...........................................................................................................................................

...........................................................................................................................................
[2]

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(c) Sodium chloride affects the activity of the enzyme catalase.

This enzyme catalyses the break-down of hydrogen peroxide to release water and oxygen
gas.

catalase
2H2O2 2H2O + O2

hydrogen peroxide water oxygen

Plan an experiment to determine the effect of sodium chloride concentration on the volume of
oxygen gas produced during this reaction.

...................................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................

............................................................................................................................................. [6]

(d) The enzyme catalase is a protein.

State the test for protein and state the result of a positive test.

test ............................................................................................................................................

...................................................................................................................................................

positive test result .....................................................................................................................

...................................................................................................................................................
[2]

[Total: 20]

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BLANK PAGE

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2 (a) The photograph in Fig. 2.1 shows a leaf from a European holly tree (Ilex aquifolium).

prickle

Fig. 2.1

(i) Draw a large diagram of the holly leaf shown in Fig. 2.1.

Do not label your drawing.

[4]

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(ii) The line PQ on Fig. 2.1 shows the length of the leaf.

Measure the length of line PQ on Fig. 2.1. Include the unit.

length of line PQ ................................................

Draw a line on your drawing in the same position as line PQ in Fig. 2.1.

Measure the length of the line you have drawn. Include the unit.

length of the line on your drawing ................................................

Calculate the magnification of your drawing using your measurements and the formula.

length of the line on your drawing

magnification =
length of line PQ in Fig. 2.1
Space for working.

................................................................
[3]

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(b) Students investigated the relationship between the number of prickles on holly leaves and the
height of the leaves above the ground.

• The students collected leaves from a total of five holly trees.

• The students took leaves from seven different heights on each holly tree.
• They collected 10 leaves from each height on each tree.
• They counted the number of prickles on each of the holly leaves and calculated the
average number of prickles per leaf at each height.

The results of the investigation are shown in Table 2.1.

Table 2.1

height above ground on average number of

each holly tree / m prickles per leaf

0.5 18

1.0 14

1.5 13

2.0

3.0 8

4.0 3

5.0 1

(i) The students counted a total of 614 prickles on the leaves collected at a height of
2.0 metres.

Calculate the total number of leaves collected at 2.0 metres above ground ........................

Then calculate the average number of prickles per leaf.

Give your answer to the nearest whole number.

Space for working.

................................................................
[3]

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(ii) State two ways the students ensured that they collected a representative sample of
leaves.

1 ........................................................................................................................................

...........................................................................................................................................

2 ........................................................................................................................................

...........................................................................................................................................
[2]

(iii) Plot a line graph on the grid to show the data in Table 2.1. Include a line of best fit.

[4]

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(c) Fig. 2.2 shows another holly leaf.

Fig. 2.2

Count and record the number of prickles on the leaf shown in Fig. 2.2.

Use the information in Table 2.1 or your graph in 2(b)(iii) to estimate the height on the tree
from which this holly leaf was collected.

number of prickles ......................................

estimated height on the holly tree ...................................... m

[2]

(d) Leaves contain starch.

State the solution used to test for starch and give the result of a positive test.

solution .....................................................................................................................................

...................................................................................................................................................

positive test result .....................................................................................................................

...................................................................................................................................................
[2]

[Total: 20]

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