Igcse 6677
Igcse 6677
Igcse 6677
* 1 0 8 7 3 3 3 8 4 3 *
BIOLOGY 9700/33
Paper 3 Advanced Practical Skills 1 February/March 2020
2 hours
You will need: The materials and apparatus listed in the confidential instructions
INSTRUCTIONS
● Answer all questions.
● Use a black or dark blue pen. You may use an HB pencil for any diagrams or graphs.
● Write your name, centre number and candidate number in the boxes at the top of the page.
● Write your answer to each question in the space provided.
● Do not use an erasable pen or correction fluid.
● Do not write on any bar codes.
● You may use a calculator.
● You should show all your working and use appropriate units.
INFORMATION
● The total mark for this paper is 40.
● The number of marks for each question or part question is shown in brackets [ ].
Total
DC (JC/CB) 186547/3
© UCLES 2020 [Turn over
2
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Before you proceed, read carefully through the whole of Question 1 and Question 2.
Plan the use of the two hours to make sure that you finish the whole of Question 1 and Question 2.
1 Yeast cells contain an enzyme, catalase, which catalyses the release of oxygen from the substrate
hydrogen peroxide.
You will investigate the effect of temperature on the activity of catalase in yeast suspension Y.
Table 1.1
If any of Y or H comes into contact with your skin, wash off immediately under cold water.
(a) (i) Think about the hazards of using the materials in Table 1.1.
State whether the risk of using hydrogen peroxide solution, H, is low, medium or high.
risk .....................................................................................................................................
reason ...............................................................................................................................
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[1]
(ii) You will test the activity of catalase in yeast suspension Y at room temperature and at
other temperatures up to a maximum of 70 °C.
State the room temperature and the other temperatures you will use in your investigation.
4. Take up 1.0 cm3 of hydrogen peroxide solution H into the same syringe, as shown in Fig. 1.1.
pull
plunger of syringe
yeast suspension Y
hydrogen peroxide
solution H
Fig. 1.1
syringe barrel
mixture of yeast suspension Y
and hydrogen peroxide solution H
drop
test-tube
Fig. 1.2
Oxygen is released as hydrogen peroxide is broken down by catalase in the yeast suspension.
This pushes some of the yeast suspension out of the nozzle of the syringe, which falls as drops
into the test-tube.
6. Observe the release of drops from the syringe as the reaction continues.
(iii) Decide how you could determine the rate at which drops are released from the syringe.
The method that you decide should take no longer than two minutes.
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..................................................................................................................................... [2]
You will now test the activity of catalase in yeast suspension Y at room temperature by finding the
rate at which drops are released from the syringe.
7. Empty the syringe and test-tube into the container labelled For waste.
8. Wash out the syringe using the water in the container labelled For washing.
11. Wipe the syringe to remove any yeast suspension on the outside.
12. Take up 1.0 cm3 of hydrogen peroxide solution H into the same syringe, as shown in Fig. 1.1.
13. Put the syringe into the test-tube, as shown in Fig. 1.2.
14. Take the measurements stated in (a)(iii) and record these measurements in (a)(iv).
You will now test the activity of catalase in yeast suspension Y at the other temperatures you
decided in (a)(ii). You must start with the lowest of these temperatures.
16. Use the Bunsen burner to heat the beaker containing yeast suspension Y. Stir gently while
heating.
Stop heating as soon as the temperature of the yeast suspension in Y reaches the required
temperature stated in (a)(ii).
18. Repeat step 16 and step 17 for each of the other temperatures you decided in (a)(ii).
[5]
A student investigated the effect of the concentration of hydrogen peroxide solution on the
activity of catalase.
(v) State the concentrations of hydrogen peroxide solution that the student could prepare to
provide a suitable range for this investigation.
Outline how these concentrations could be prepared from 5.0% hydrogen peroxide
solution.
concentrations ...................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
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[2]
(vi) The student standardised the volume and concentration of the catalase solution.
variable ..............................................................................................................................
...........................................................................................................................................
[1]
(b) A scientist carried out an investigation into the effect of pH on the activity of a different enzyme.
Table 1.2
enzyme activity
pH
/ arbitrary units (au)
3.2 1.50
4.8 22.75
5.7 29.00
6.5 24.50
7.5 9.00
(i) Plot a graph of the data in Table 1.2 on the grid in Fig. 1.3.
Fig. 1.3
[4]
© UCLES 2020 9700/33/F/M/20
9
(ii) Use your graph to estimate the enzyme activity when the pH is 4.0.
(iii) Describe the trend in the results shown by your graph plotted on Fig. 1.3.
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[Total: 22]
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2 Starch grains from different types of plant differ in size and shape. Some starch grains have rings
on their surfaces.
You will now observe and draw starch grains from one type of plant.
1. Put one clean and dry microscope slide onto a paper towel.
2. Using a pipette, put a few drops of S onto the slide. S is a suspension of starch grains.
3. Cover the drops of S on the slide with a coverslip and use a paper towel to remove any
excess suspension.
4. Use the microscope to find and observe the starch grains on the slide.
You may need to reduce the amount of light entering the microscope and adjust the fine focus
to observe the starch grains clearly.
5. Select, from a single field of view, four starch grains that show different sizes and features.
(a) (i) Make a large drawing of the four starch grains that you have selected.
[4]
6. Remove the slide from the microscope and place it on a paper towel.
Fig. 2.1 shows some different types of starch grains and patterns on the surface of the starch
grains.
J K L M
Fig. 2.1
(ii) Use the diagrams in Fig. 2.1 to identify which of the starch grains, J, K, L or M, matches
most closely the starch grains drawn in (a)(i).
A student calibrated the eyepiece graticule in a light microscope using a stage micrometer
scale.
The student used the microscope to observe and draw three starch grains to the same scale.
The student drew a line across the length of each drawing of a starch grain. The student's
drawings are shown in Fig. 2.2.
N2
N1
N3
Fig. 2.2
(iii) Find the mean image length of the three starch grains drawn by the student, along the
lines shown in Fig. 2.2.
Show all the steps in your working and use appropriate units.
(iv) When viewed using the microscope, the student found that starch grain N1 measured
4 eyepiece graticule units along the position of the line drawn in Fig. 2.2.
Use this information and your answer to (a)(iii) to calculate the mean actual length of
the three starch grains in Fig. 2.2.
Show all the steps in your working and use appropriate units.
(b) Fig. 2.3 is a photomicrograph of a transverse section of a plant root containing starch grains.
The section has been stained with iodine solution.
Fig. 2.3
You are expected to draw the correct shapes and proportions of the different tissues.
Draw a large plan diagram of the transverse section of the whole root shown in Fig. 2.3.
Use one ruled label line and label, with the letter G, the tissue that contains most of the starch
grains.
[5]
(c) Fig. 2.4 is a photomicrograph of a stained transverse section through a root of a different type
of plant.
Fig. 2.4
Fig. 2.5 is a photomicrograph of the same root section that is shown in Fig. 2.3.
Fig. 2.5
Prepare an appropriate table so that it is suitable for you to record the observable differences
between the root in Fig. 2.4 and the root in Fig. 2.5.
Record the observable differences, other than differences in colour, in your table.
[4]
[Total: 18]
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