Sylabus For Enzyme Technology

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University of Gondar

Institute of Biotechnology
Department of Environmental and Industrial Biotechnology
Industrial Biotechnology Stream
A Course Guide for Enzyme Biotechnology
Academic Year: 2020/2021
I. Course Information
Course Title : Enzyme Biotechnology
Course Code : Biot.632
Credit Hours : 2 credit hours/ week
Program : MSc
II. Instructor`s Contact Information
Name : Tamene M. (PhD, Assistant Professor)
Office : PG Building, First Floor, Last Room
Email : [email protected]/[email protected]
III. Course description
The course will give a basic understanding of enzyme function and will teach theoretical and
experimental methods that form the basis for enzyme characterization. The contents that will be covered
includes: catalytic principles and reaction mechanisms of enzymes, binding energy and catalysis, enzyme
kinetics (steady-state and pre steady-state), inhibition (reversible and irreversible) and its applications,
production of enzymes, practical methods in enzymology (for example studies of enzyme kinetics and
reaction mechanisms, detection of intermediates, active-site titration), environmental effects on enzymes
(for example pH, temperature, organic solvents), enzyme immobilization, extemozymes,
protein/enzyme engineering (aims and strategies). The course has many practical steps as in depth
planning of experiments, information search emphasized on structures.
Course Objectives
At the end of this course, the students will be able to:
 understand the catalytic principles and reaction mechanisms of enzymes
 learn how enzyme inhibition (reversible and irreversible) occurs and its applications

 know how to immobilize enzyme

 discuss in detail about extemozymes

 get exposure of wide applications of enzymes and their future potential.

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IV. Course Content
1. Introduction
2. Catalytic principles and reaction mechanisms of enzymes
3. Enzyme inhibition (reversible and irreversible) and its applications
4. Production of enzymes
5. Enzyme immobilization
6. Extemozymes
7. Protein/enzyme engineering : aims and strategies
8. Applications of enzymes
VI. Assessment
i. Presentation ---------------------30%
ii. Mid exam ------------------------20%
iii. Final exam------------------------50%
VII. References
1. Khan, M. Y., Khan, Farha.2015. Principles of Enzyme Technology. PHI Learning Pvt.
Ltd., Aug 31, 2015
2. S. Shanmugam.2009. Enzyme Technology. I. K. International Pvt Ltd.
3. Martin F. Chaplin, C. Bucke.2003. Enzyme Technology.Cambridge University Press.
4. Pandey, A., Webb, C., FERNANDES, M., Larroche, C. (Eds.).2006. Enzyme
Technology. Springer-Verlag New York.
Approval section
Course Instructor
_________________________ __________________ ____________________
Name Date Signature
Department head
________________________ __________________ ____________________
Name Date Signature

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