BIOA01 Statistical Worksheet - F2022

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BIOA01H3 F FLR Student’s t-Test Worksheet WORKSHEET – Fall 2022

Use this worksheet to help you work through your statistical analysis for your FLR. You do not need to
submit this worksheet with your report. To complete this analysis, you will need to download your
practical’s complete data set that has been posted to Quercus. Remember, your data set will include data
from your lab practical and one other lab practical. You will be completing one t-test comparing the
photosynthetic rate (mL/min) of the control plants with the photosynthetic rate (mL/min) of the
experimental plants. In your data set, you should have 16 replicates for each of the control and
experimental groups.
t-test comparing Photosynthetic Rate (mL/min)

Control Experimental
TREATMENT (0.5% NaHCO3 only) (0.5% NaHCO3 + DCMU)

Sample Size (n)

Mean (x)

Standard Deviation (s)

Variance (s2)

SEM (√ s2 / n )

Calculated t -Statistic

Mean1 - Mean2

√ SEM12 + SEM 22

Degrees of Freedom
df = (n1 + n2) - 2

Critical t -Statistic from


t -Test Table
for two-tail test
(cut-off p-value 0.05)
Calculated t-Statistic Calculated t-statistic:

Cut-off p-value Cut-off p-value: 0.05

Actual p-value Actual p-value:

Is your actual p-value <, >, =0.05?

Conclusion (reject or fail


to reject H0)

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