Biology 107

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Biology 107 Practical

A laboratory is a building or a room where scientific experiments, analysis and research are been
carried out.

Rules in the Laboratory

1) Do not go into the laboratory without permission


2) Never run or rush about in the laboratory
3) Never put anything into the mouth in the laboratory
4) Never interfere with equipments
5) Never take anything from the laboratory  without permission
6) Never throw things in the laboratory
7) All accidents and breakages, however small must be reported to your instructor at once.
8) Do not eat sweet or chewing gum or any other edible materials in the laboratory
9) Never interfere with electrical fittings,  gas and water taps in the laboratory
10) Always wear your laboratory coat in the laboratory

Recording Precautions
1) Pencils should be sharpened and of HB quality. No coloured Pencils should be used.
2) Drawings  must be large enough.
3) Shading and colours should be avoided
4) It is advisable that proper identification be made of all biological specimens drawn, all biological
5) Draw what you see and not what you think you should see and certainly not a textbook copy
6) Every drawing should have a title, drawing aim, apparatus used, procedures, observation and
magnification (product of objective lens and eye piece)

Microscope

It is one of the most important and often used instruments in the lab

It is an optical instrument used for viewing very small and minute objects. It allows the users to peer
into the world of the cell. It extends the vision of the observer more than a thousand times and the
most commonly used is the light Microscope

Types
1) Monocular Microscope: one eye lens
2) Binocular Microscope : two eye lens

Part of the Microscope and their Uses

1)Eye Piece: It contains a lens called the ocular that further magnifies the specimen by times 10 (x10).
It is also the lens in which the viewer looks through to see the specimen.

2) Rotating Head: It contains mirror and allows the body to rotate at 360°

3) The Arm: It connects the body tube to the base and the Microscope is carried with the arm.
4) Diopter Adjustment (it comes with the Binocular) : It is useful as a means to change focus on the
eyepiece so as to connect for any differences in the vision between the two eyes.

5) Objective Lens (4 tubes): They come by (x4, x10, x40 and x100(immersion lens)  It is called
immersion lens without adding immersion oil it can't  be viewed through ). It produces most of the
magnification an they are the lenses that are closest to the specimen

6) Revolving Nose Piece: It revolves the objective lenses. It is also used to change from one objective
lens to the other.

7) Mechanical Stage: It holds the slide and contains an opening that allows light to pass through the
specimen on its way to the objective lens.

8) Iris Diaphragm :Beneath the stage, it adjusts or controls the light intensity that reaches the
specimen.

9) Condenser : It's a lens system found beneath the stage. It is used to gather and focus light from the
illuminator into the specimen. It is a glass or mirror right under the stage.

10) Coarse Adjustment Knob: It moves the stage up and down and it's also used to locate the
specimen.

11) Fine Adjustment Knob: It is used to make small focus adjustment especially when using high
power objective. It increases the detail of the specimens. It is the smaller knob (smaller than the
Coarse adjustment knob)

12) Stage Clip: It is used to hold the slide firmly.

13) Base: It supports the microscope and it is where the illuminator is being located.

14) Illuminator: It is the source of light in the microscope and there is a bulb inside.

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