Chem Proj

Delhi Public School
CERTIFICATE
This is to certify that KRITIN PANDA, bona fide

student of class XII -A has done a project on
Nutrients Present In The Fruits in his practical
pursuance of AISSCE for the session 2023-24 and
has completed the project satisfactorily.
Mr. KVS Ramprasad Signature of Mrs. Deepanwita Das

Project supervision external examiner (Principal)
Dept. of Chemistry
ACKNOWLEDGEMENT
I sincerely express my deep gratitude and

indebtedness to the principal Mrs. Deepanwita Das
and my project guide Mr. Ramprasad, science
department of chemistry and Mrs. Geetanjali Rath,
Lab Assignment Delhi Public School, Damanjodi
without their Inspiration, guidance, supervision and
keen interest, this piece of investigatory work would
not have been possible.
Lastly, I thank all those have Encouraged me to go

ahead and complete my Project work.
CONTENTS
• AIM OF THE EXPERIMENT
• APPARATUS REQUIRED
• CHEMICALS REQUIRED
• THEORY
• CHEMISTRY INVOLVED
• TABULATION
• CONCLUSIONS
AIM OF THE PROJECT
The main objective of this project is to find out various constituents of
fruits. Hence, to plan a balanced diet which provides us nutrition and
keeps us healthy.
APPARATUS REQUIRED:
• Test-tube
• Test-tube holder
• Bunsen burner
• Dropper
CHEMICALS REQUIRED:
• Iodine solution
• Fehling A and B
• Tollen’s reagent
• Ninhydrin
• Molisch reagent
• Potassium Hydrogen Sulphide
• Concentrated Sulphuric acid
• KHSO4
THEORY
The presence of carbohydrates, proteins and fats in any food is detected by performing their
respective tests. The foods are tested for the presence of nutrients by performing certain tests.
TEST FOR CARBOHYDRATES

Carbohydrates are polyhydroxy aldehydes or ketones or the compound which yield polyhydroxy
aldehydes or ketones on hydrolysis.
MOLISCH'S TEST
Molisch's reagent is a 10% alc. Solution of naphthalene concentrated. It converts
carbohydrates into its derivatives which further reacts with Molisch reagent to
give a coloured compound. Take sample in test tube, a few drops of Molisch reagent is added
and the conc. H2S04 is along the wall of the test tube. Red-violet precipitate is observed if
carbohydrates are present.
IODINE TEST
This test is to test the presence of starch. To the sample 2-3 drops of Iodine sol. is added. Violet-
Blue confirms presence of starch.
3.FEHLING'S TEST
Fehling's sol. Is a mixture of Fehling A and B. Fehling A is CuS04 sol. Whereas
Fehling B is a mixture of Rochelle's solution dissolved in NaOH. The formation of

Red precipitate confirms the presence of carbohydrates due to formation of CuO.
4.BENEDlCT'S TEST
Here benedict's reagent is used. The given sample was taken in a test tube and reagent was
added and kept still. Formation of Red precipitate confirms carbohydrates.
TOLLEN'S TEST
Tollens's reagent is ammoniacal silver nitrate sol. The sample was taken in a test tube and 2-3 drops
of dil. NaOH was added. A brown precipitate will be formed and the NH3 is added drop wise such
that the brown colour slowly disappears. Then it's kept in water bath. Formation of silver mirror
along the walls of the test tube indicates the presence of carbohydrates.
TEST FOR PROTEINS
1.XANTHOPROTEIC TEST
A little sample was taken in a test tube and then conc. H2S04 is added to it. Appearance of bluish
precipitate confirms the presence of proteins.
2.BURETTE TEST
The given sample was taken in a test tube and NaOH was added to it and a drop of CuS04 sol. Was
added to it. An appearance of bluish precipitate confirms protein in it.
3. NINHYDRIN TEST
The given sample is taken in a test tube and a few crystals of ninhydrin are added to it and then kept
in water bath.
TEST FOR FATS
1.SOLUBILITY TEST
The given sample is taken in a test-tube and water was added to it. Formation of insoluble mass
which is soluble in organic solvent confirms the presence if fats.
2.TRANSLUCENT SPOT TEST

The sample is taken in a piece of paper and rubbed. Formation of translucent spot in paper confirms
fats.
3.ACROLEIN TEST
A small amount of sample was taken and KHS04 is added to it and heated. Release of irritating smell
confirms the presence of fats
CONCLUSION
Hence, the various nutrients present in fruits like apple,
grapes, pear, lemon, orange and pomegranate juice can be
found out using some of the tests.
From the above tests we conclude that fruits should form an

important part of our diet as well as cereals. They are
important for a balanced diet. The deficiency of any of these
can cause metabolic disorders.

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Delhi Public School

This is to certify that KRITIN PANDA, bona fide

Mr. KVS Ramprasad Signature of Mrs. Deepanwita Das

I sincerely express my deep gratitude and

Lastly, I thank all those have Encouraged me to go

TEST FOR CARBOHYDRATES

Fehling B is a mixture of Rochelle's solution dissolved in NaOH. The formation of

TEST FOR FATS

2.TRANSLUCENT SPOT TEST

From the above tests we conclude that fruits should form an

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