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A-level
BIOLOGY
Paper 1

Thursday 4 June 2020 Morning Time allowed: 2 hours

Materials For Examiner’s Use
For this paper you must have:
• a ruler with millimetre measurements Question Mark
• a scientific calculator. 1
2
Instructions
3
• Use black ink or black ball-point pen.
• Fill in the boxes at the top of this page. 4
• Answer all questions. 5
• You must answer the questions in the spaces provided. Do not write 6
outside the box around each page or on blank pages.
7
• If you need extra space for your answer(s), use the lined pages at the end of
this book. Write the question number against your answer(s). 8
• Show all your working. 9
• Do all rough work in this book. Cross through any work you do not want
10
to be marked.
TOTAL
Information
• The marks for the questions are shown in brackets.
• The maximum mark for this paper is 91.

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Answer all questions in the spaces provided. box

0 1 Figure 1 shows a cell from the lining of the ileum specialised for absorption of
products of digestion.

SGLT1 is a carrier protein found in the cell-surface membrane of this cell, it transports
glucose and sodium ions (Na+) into the cell.

Figure 1

0 1 . 1 The action of the carrier protein X in Figure 1 is linked to a membrane-bound ATP

hydrolase enzyme.

Explain the function of this ATP hydrolase.

[2 marks]

0 1 . 2 The movement of Na+ out of the cell allows the absorption of glucose into the
cell lining the ileum.

Explain how.
[2 marks]

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0 1 . 3 Describe and explain two features you would expect to find in a cell specialised for box

absorption.
[2 marks]

Question 1 continues on the next page

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Figure 2 is a diagram of one SGLT1 carrier protein.

Figure 2

0 1 . 4 Draw phospholipids on Figure 2 to show how the carrier protein, SGLT1, would fit into
the cell-surface membrane.

Do not draw more than eight phospholipids.

[2 marks]

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0 1 . 5 Figure 2 shows the SGLT1 polypeptide with NH2 at one end and COOH at the box

other end.

Describe how amino acids join to form a polypeptide so there is always NH2 at
one end and COOH at the other end.

You may use a diagram in your answer.

[2 marks]

Space for diagram:

10

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0 2 To study lipid digestion, a scientist placed a tube into the gut of a healthy
20-year-old man. The end of the tube passed through the stomach but did not reach
as far as the ileum.

The scientist fed the man a meal containing triglycerides through the tube.
The scientist also used the tube to remove samples from the man’s gut at intervals
after the meal.

The scientist measured the type of lipid found in the samples. Some of her results are
shown in Table 1.

Table 1

Sample Time of collection Concentration of Concentration of

after meal / min fatty acids / mg cm−3 triglycerides / mg cm−3

A 45 2.7 0.6
B 75 3.3 0.0

0 2 . 1 Use your knowledge of lipid digestion to explain the differences in the results for
samples A and B shown in Table 1.

You should assume that no absorption had occurred.

[3 marks]

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0 2 . 2 After collecting the samples, the scientist immediately heated them to
70 °C for 10 minutes.

Explain why.
[2 marks]

0 2 . 3 Describe the role of micelles in the absorption of fats into the cells lining the ileum.
[3 marks]

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0 3 Figure 3 shows pressure and blood flow during the cardiac cycle in a dog.

Figure 3

0 3 . 1 At P on Figure 3, the pressure in the left ventricle is increasing. At this time, the rate
of blood flow has not yet started to increase in the aorta.

Use evidence from Figure 3 to explain why.

[2 marks]

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0 3 . 2 At Q on Figure 3 there is a small increase in pressure and in rate of blood flow in the box

aorta.
Explain how this happens and its importance.
[2 marks]

0 3 . 3 A student correctly plotted the right ventricle pressure on the same grid as the left
ventricle pressure in Figure 3.

Describe one way in which the student’s curve would be similar to and one way it
would be different from the curve shown in Figure 3.
[2 marks]

Similarity

Difference

0 3 . 4 Use information from Figure 3 to calculate the heart rate of this dog.
[1 mark]

Heart rate beats minute–1 7

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0 4 Anthocyanins are coloured pigments found in the cell vacuole of some plant cells.
Anthocyanins cannot move across undamaged cell membranes.

A student investigated how to extract anthocyanins from blueberries.

She mixed 10 g of crushed, fresh blueberries with 100 cm3 of extraction solvent for
1 hour.

She investigated three different extraction solvents:

• E – Ethanol, water and acid

• F – Ethanol and water
• G – Water

0 4 . 1 When making up extraction solvent E, the student used a volume ratio of

70:30:1 ethanol:water:acid.

Tick () one box that shows the most appropriate volumes she would use to make up
100 cm3 of extraction solvent E.
[1 mark]

63.6 cm3 ethanol, 27.3 cm3 water, 9.1 cm3 acid

69.3 cm3 ethanol, 29.7 cm3 water, 1.0 cm3 acid

70.0 cm3 ethanol, 30.0 cm3 water, 1.0 cm3 acid

70.7 cm3 ethanol, 30.3 cm3 water, 1.0 cm3 acid

0 4 . 2 The student kept constant:

• the mass of fresh blueberries

• the volume of extraction solvent
• the time for the mixture to stand.

Name two other variables the student should have kept constant during this
investigation.
[2 marks]

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0 4 . 3 After 1 hour, the student filtered the samples. box

She placed the filtrate in a colorimeter and measured the light absorbance.

Her results are shown in Figure 4.

Figure 4

Use your knowledge of membrane structure to explain the results in Figure 4.

[4 marks]

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0 4 . 4 A different student did this investigation. He did not have a colorimeter. box

Describe a method this student could use to prepare colour standards and use them
to give data for the total anthocyanin extracted.
[3 marks]

10

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ANSWER IN THE SPACES PROVIDED

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0 5 . 1 Describe the role of DNA polymerase in the semi-conservative replication of DNA.
[2 marks]

Figure 5 shows the percentage of rat cells undergoing DNA replication. Some cells
contained a protein called cyclin D and some cells did not contain cyclin D. All cells
were in early interphase at time 0

Figure 5

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0 5 . 2 It took less time for 25% of cells with cyclin D to be undergoing DNA replication than box

for 25% of cells without cyclin D.

Use Figure 5 to calculate this time difference as a percentage decrease.

Show your working.

[2 marks]

Answer %

0 5 . 3 Cyclin D stimulates the phosphorylation of DNA polymerase, which activates the

DNA polymerase.

Describe how an enzyme can be phosphorylated.

[2 marks]

0 5 . 4 Some tumour cells contain higher than normal concentrations of cyclin D.

Use Figure 5 to suggest why higher than normal concentrations of cyclin D could
result in a tumour.
[2 marks]

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0 6 . 1 Particulate matter is solid particles and liquid particles suspended in air. Polluted air box

contains more particulate matter than clean air.

A high concentration of particulate matter results in the death of some

alveolar epithelium cells. If alveolar epithelium cells die inside the human body they
are replaced by non-specialised, thickened tissue.

Explain why death of alveolar epithelium cells reduces gas exchange in human lungs.
[3 marks]

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Scientists grew alveolar epithelium cells and exposed the epithelium cells to different box

concentrations of particulate matter. They calculated the percentage of these

alveolar epithelium cells that died after 24 hours of exposure to particulate matter.
Their results are shown in Figure 6.

Figure 6

0 6 . 2 Do the data in Figure 6 show a linear relationship between concentration of

particulate matter and percentage of dead cells?

Use suitable calculations to justify your answer.

[2 marks]

5
Space for your calculations:

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0 7 . 1 Alpha-gal is a disaccharide found in red meat.

Alpha-gal is made of two galactose molecules. Galactose has the chemical formula
C6H12O6

Give the chemical formula for the disaccharide, alpha-gal, and describe how it is
formed from two galactose molecules.
[2 marks]

Formula

Description

0 7 . 2 Some people eat red meat for many years without having any reaction, then have an
allergic reaction to the alpha-gal in red meat.

An allergic reaction is caused by an immune response.

Draw a labelled diagram of an antibody and identify the specific alpha-gal binding site.
[3 marks]

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0 7 . 3 A tick is a small animal that bites humans and feeds on their blood. This results in box

proteins from the tick saliva entering the human body.

Scientists have suggested one hypothesis for the allergic reaction to alpha-gal in
red meat. They think that an earlier immune response to a tick bite can cause a
person to have an allergic reaction to alpha-gal in red meat.

Suggest how one antibody can be specific to tick protein and to alpha-gal.
[2 marks]

Question 7 continues on the next page

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0 7 . 4 Scientists took blood samples from one man over several weeks and measured the box

concentration of antibody in the man’s blood. During this time, the man had two tick
bites and had an allergic reaction to alpha-gal in red meat.

The scientists’ results are shown in Figure 7.

Figure 7
This figure has been removed due to third-party copyright restrictions.

The scientists’ hypothesis was that an earlier immune response to tick protein causes
the allergic reaction.

Consider whether Figure 7 supports this hypothesis.

[3 marks]

10

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0 8 . 1 Complete Table 2 to show three differences between DNA in the nucleus of a
plant cell and DNA in a prokaryotic cell.
[3 marks]

Table 2

DNA in the nucleus of a plant cell DNA in a prokaryotic cell

0 8 . 2 Scientists investigated the genetic diversity between several species of sweet potato.
They studied non-coding multiple repeats of base sequences.

Define ‘non-coding base sequences’ and describe where the non-coding multiple
repeats are positioned in the genome.
[2 marks]

Question 8 continues on the next page

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The percentage similarities in the non-coding multiple repeats of base sequences of box

four species of sweet potato are shown in Table 3.

Table 3

Percentage similarity between non-coding multiple

Species of sweet repeat base sequences
potato
C L R T
C 53.5 25.7 59.7
L 53.5 33.4 53.7
R 25.7 33.4 36.6
T 59.7 53.7 36.6

0 8 . 3 Use the information in Table 3 to complete the phylogenetic tree shown in Figure 8.

Write the letter that represents the correct species into each box.
[1 mark]
Figure 8

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0 8 . 4 The scientists studied five individuals from each species. Within the five individuals of box

species T they found a percentage similarity of 66%.

Use Table 3 to evaluate how this information affects the validity of the
phylogenetic tree.
[2 marks]

Turn over for the next question

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0 9 Scientists investigated stomatal density on leaves of one species of tree.

Figure 9 shows three examples of the square fields of view the scientists used to
calculate a mean stomatal density.

Figure 9

0 9 . 1 Calculate the mean stomatal density in the three fields of view in Figure 9.

Give your answer as number of stomata per mm2

Show your working.

[2 marks]

Stomatal density per mm2

Question 9 continues on the next page

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The scientists used leaves from individual trees that had grown in different areas of box
the world in different years. Each tree had grown in an area and year with known
carbon dioxide concentration.

Their results are shown in Figure 10.

Figure 10

0 9 . 2 Give a null hypothesis for this investigation and name a statistical test that would be
appropriate to test your null hypothesis.
[2 marks]

Null hypothesis

Statistical test

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0 9 . 3 From 1910 to 2000, the carbon dioxide concentration in the atmosphere increased box

from 300 parts per million to 365 parts per million.

Use Figure 10 to calculate the mean rate of change in stomatal density from
1910 to 2000.

Give your answer as number of stomata per mm2 per 10-year period.

Show your working.

[2 marks]

Number of stomata per mm2 per 10-year period

0 9 . 4 A journalist saw Figure 10 and suggested that future increases in atmospheric

carbon dioxide concentration could result in less transpiration.

Evaluate his suggestion.

[4 marks]

10

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1 0 . 1 Describe how mRNA is formed by transcription in eukaryotes. box

[5 marks]

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1 0 . 2 Describe how a polypeptide is formed by translation of mRNA. box

[6 marks]

Question 10 continues on the next page

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1 0 . 3 Define ‘gene mutation’ and explain how a gene mutation can have: box

• no effect on an individual
• a positive effect on an individual.
[4 marks]

15

END OF QUESTIONS

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Question Additional page, if required.
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