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    LectureP7 HND 410

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    MARIA TARIQ

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    LectureP7 HND 410

    Uploaded by

    MARIA TARIQ
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    Original Title

    LectureP7-HND-410 (2)

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    Copyright:

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    5 views3 pages

    LectureP7 HND 410

    Uploaded by

    MARIA TARIQ

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 3

    Practical 7

    Isolation of Bacteria by Streak Plate Method

    The study of microbial characteristics starts with the isolation of pure culture. A pure culture
    contains a single kind of microorganism. To isolate a pure culture of a desired organism,
    select suitable medium. The most widely used methods of obtaining a pure culture are
    following:

    1. Streak Plate Method


     This method was developed by two bacteriologists, Leoffler and Gaffkey in the

    laboratory of Robert Koch. This method is routinely employed for the isolation of

    bacteria in pure culture.

     In this method a sterilized inoculating loop or transfer needle is dipped into a suitable

    diluted suspension of microorganisms which is then streaked on the surface of an

    already solidified agar plate to make a series of parallel, non-overlapping streaks. The

    process is known as streaking and the plate so prepared is called a streak plate.

     The main objective of the streak plate method is to produce well separated colonies of

    bacteria from concentrated suspensions of cells.

     A sterilized inoculating needle with a loop made up of either platinum or nichrome wire

    is used for streaking. One loopful of specimen is transferred onto the surface of the agar

    plate in a sterile petri dish and streaked across the surface in the form of a zig-zag line.

    This process is repeated thrice to streak out the bacteria on the agar plate so that some

    individual bacteria are separated from each other.


     The first streak will contain more organisms than the second and the second more than

    the third and so on. The last streaks should thin so on. The last streaks should thin out

    the culture sufficiently to give isolate colonies.

     The successful isolation depends on spatial separation of single cells. Each colony

    usually represents the growth from a single organism when such a plate is incubated

    colonies will appear on the surface of the medium.

     Because of the high concentration of water in agar, some water of condensation forms in

    petri plate during incubation. Moisture is likely to drip from the cover to the surface of

    the agar and spread out, resulting in a confluent mass of growth and running individual

    colony formation. To avoid this, petri plates are routinely incubated in inverted position.

     Pure colonies can be obtained from well isolated colonies by transferring a small portion

    of each to separate culture media.

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