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    Streak Plate and Spread Plate Inoculation: Activity No.6

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    Francis Valdez
    This document describes three techniques for isolating bacterial colonies on agar plates: the spread plate technique, streak plate technique, and dilution streak technique. The spread plate technique involves spreading bacteria evenly over the plate surface to allow isolated colony growth. The streak plate technique streaks bacteria across the plate with a loop to leave fewer cells with each streak, allowing single colony isolation. The dilution streak technique uses serial dilutions and streaking to isolate and estimate bacterial numbers. All techniques rely on incubating single bacterial cells to grow into visible pure colonies derived from a single clone for identification purposes.

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    Streak Plate and Spread Plate Inoculation: Activity No.6

    Uploaded by

    Francis Valdez
    9 views3 pages
    This document describes three techniques for isolating bacterial colonies on agar plates: the spread plate technique, streak plate technique, and dilution streak technique. The spread plate technique involves spreading bacteria evenly over the plate surface to allow isolated colony growth. The streak plate technique streaks bacteria across the plate with a loop to leave fewer cells with each streak, allowing single colony isolation. The dilution streak technique uses serial dilutions and streaking to isolate and estimate bacterial numbers. All techniques rely on incubating single bacterial cells to grow into visible pure colonies derived from a single clone for identification purposes.

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    Activity no6 review

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    This document describes three techniques for isolating bacterial colonies on agar plates: the spread plate technique, streak plate technique, and dilution streak technique. The spread plate technique involves spreading bacteria evenly over the plate surface to allow isolated colony growth. The streak plate technique streaks bacteria across the plate with a loop to leave fewer cells with each streak, allowing single colony isolation. The dilution streak technique uses serial dilutions and streaking to isolate and estimate bacterial numbers. All techniques rely on incubating single bacterial cells to grow into visible pure colonies derived from a single clone for identification purposes.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    9 views3 pages

    Streak Plate and Spread Plate Inoculation: Activity No.6

    Uploaded by

    Francis Valdez
    This document describes three techniques for isolating bacterial colonies on agar plates: the spread plate technique, streak plate technique, and dilution streak technique. The spread plate technique involves spreading bacteria evenly over the plate surface to allow isolated colony growth. The streak plate technique streaks bacteria across the plate with a loop to leave fewer cells with each streak, allowing single colony isolation. The dilution streak technique uses serial dilutions and streaking to isolate and estimate bacterial numbers. All techniques rely on incubating single bacterial cells to grow into visible pure colonies derived from a single clone for identification purposes.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 3

    Activity no.

    6
    Streak plate and spread plate inoculation

    The streak-plate method allows CFUs to be isolated by streaking.

    The pour-plate technique isolates CFUs via a series of dilutions.

    Spread plate technique,

    A. A small volume of dilute microbial suspension containing ca 30–300 cells is


    transferred to the center of an agar plate and spread evenly over the surface
    with a sterile bent-glass rod.
    B. The dispersed cells develop into isolated colonies. Because the number of
    colonies should equal the number of viable organisms in a sample, spread plates
    can be used to count the microbial population.

    Streak plate technique

    A. the original suspension can be streaked on the surface of an agar plate with a wire
    loop agar plate with a wire loop (streak-plate technique). As the streaking continues,
    fewer and fewer cells are left on the loop, and finally the loop may deposit single
    cells on the agar
    B. The plate is incubated, and any well-isolated colony is then removed, resuspended
    in sterile medium, and again streaked on agar. If a suspension (and not just a bit of
    growth from a colony or slant) is streaked, this method is just as reliable as and
    much faster than the pourplate method.
    Dilution streak technique for isolation and semiquantitation of
    bacterial colonies.
     With appropriate incubation conditions, each bacterial cell inoculated onto the agar medium
    surface will proliferate to sufficiently large numbers to be observable with the unaided eye.
     The resulting bacterial population is considered to be derived from a single bacterial cell and is
    known as a pure colony.
     In other words, all bacterial cells within a single colony are the same genus and species, having
    identical genetic and phenotypic characteristics (i.e., are derived from a single clone).
     Pure cultures are required for subsequent procedures used to identify and characterize
    bacteria.
     The ability to select pure (individual) colonies is one of the first and most important steps
    required for bacterial identification and characterization.

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