Veterinary Public Health
Veterinary Public Health
Veterinary Public Health
ACTIVITIES
LG
LO 1. Perform Community Awareness on Veterinary Public Health Issues
Introduction
Human health is inextricably linked to animal health and production. This link between human
and animal populations, and with the surrounding environment, is particularly close in
developing regions where animals provide transportation, draught power, fuel and clothing as
well as proteins (meat, eggs and milk). In both developing and industrialized countries, however,
this can lead to a serious risk to Public Health with severe economic consequences. A number of
communicable diseases (zoonoses) are transmitted from animals to humans. Any disease or
infection that is naturally transmissible from vertebrate animals to humans and vice-versa is
classified as zoonoses. Veterinary medicine has a long and distinguished history of
contributing to the maintenance and promotion of Public Health.
The animal itself, animal health and veterinary science are related to the physical, mental and
social well-being of humans and the contribution of Veterinary Public Health (VPH) in this field
is considered fundamental. Veterinary Public Health is a fundamental part of Public Health
whereby human health and well-being are the main objectives. Veterinary Public Health
comprises the application, efforts and knowledge of Veterinary science which contribute to
secure, promote and restore human health.
Veterinary Public Health is the field of veterinary medicine that is concerned with
safeguarding and improving the health of the human community as a whole by controlling
diseases of animals that are communicable to humans or which affect the human food chain to
the detriment of the health of the consumers. It comprises all the community efforts influencing
and influenced by the Veterinary medical arts and sciences applied to the prevention of diseases,
protection of life, and promotion of the well-being and efficiency of man. According to the WHO
definition of health the following definition of Veterinary Public Health was suggested at a
WHO meeting as "Veterinary
Public Health is the contribution to the complete physical, mental, and social well-being of
humans through an understanding and application of veterinary medical science.
Food protection
Zoonoses
• Food Microbiology
Zoonoses • Food Chemistry
Epidemiology • Meat Hygiene
• Milk Hygiene
VPH
Services
Environmental health
Each year millions of people are affected by foodborne and waterborne diseases and thousands
die, especially children in developing countries. Improper methods of food production, storage,
handling and preparation have resulted in many recognized international outbreaks. Food
animals are the reservoir for many emerging and important foodborne diseases, including those
caused by Escherichia coli O157:H7, non-typhoidal Salmonella spp., Campylobacter spp. and
Yersinia spp. VPH leadership is essential to respond to the threat posed by these diseases,
particularly in the development of sustainable, integrated safety measures for the reduction of
health risks along the entire food chain, from the point of primary production to the consumer
(i.e. the “farm-to-table” approach).
Traditionally the veterinary public health course was primarily focused on meat inspection,
but now it has been expanded to include:
Quality assurance of animal-based products to meet consumer expectations;
Knowledge of the causes, epidemiology and control of food borne zoonotic and
emerging diseases;
Adverse effects of agricultural and other human activities on food quality, animal
welfare, human health and the environment.
Other principles needed to maximize the effectiveness of any education/training/
community awareness should include:
Protect consumers against zoonotic diseases
Avoid unaesthetic products
Ensure hygienic processing, storing and marketing of meat
Avoid harmful products like antibiotics and pesticide residues
Help to identify and control infectious diseases especially notifiable diseases
Protect the economy of the country
Avoid cruelty against animals by ensuring humane animal handling and slaughter
practices
Ensure sanitary conditions in slaughterhouses
Ensure hygienic disposal of waste products.
The services of lay workers could be better utilized for providing village level basic human and
animal care. This could be done through their education and training in vaccination, presumptive
diagnosis and subsequent reporting to veterinary or medical officers. Data collection could also
be achieved and above all, educating animal owners about the importance of VPH, the risks
involved and what steps can be taken to avoid them. Education of animal owners and animal
product producers is of critical importance, because without a change in their attitude and
practices, nothing substantial can be attained.
community based VPH program, especially those involving grass roots approaches
VPH program developed specifically to identify zoonotic infections and risks for
children (9-16 years of age) working as apprentices in abattoirs. Abattoir waste water
quality assessment was also included as a measure of environmental pollution.
Food hygiene and disease prevention are fundamentally important. All personnel should be
aware of their role and responsibility in protecting food from contamination or deterioration.
Community and food handlers should have the necessary knowledge and skills to enable them to
handle food hygienically.
Community awareness activity delivery techniques
Develop and disseminate strategic visual and audio materials through Radio and
Television Channels and other means
Community awareness, participation, mobilization and Health and Hygiene Education
campaign
Behavior change training of trainer workshops
Local/ community based campaign and awareness program
Yearly reward and recognition events (with letter of appreciation, token for contribution,
and honorary certificate)
Developing different types of brochures and disseminate
Through magazines/Journals
Information Sheet 2- Conducting veterinary public health awareness activities
Public Health (VPH) is a component of public health that is devoted to the application of
professional veterinary knowledge, skills and resources to the protection and improvement of
human health. VPH contributes to the physical and social wellbeing of humans through an
understanding and application of veterinary science. Many human diseases are transmitted
through foods of animal origin especially meat, milk, eggs and fish, while others are transmitted
through contact with infected animals or materials from infected animals.
Disease is any impairment of the normal physiological function of plant and animal caused by
inorganic or pathogenic agents. Disease is caused by (i). Non-living agents which can be physical
or chemical agents. (ii). Living agents (microorganisms) such as bacteria, viruses, fungi,
protozoa, helminths, rickettsial, chlamydia etc. Environmental factors such as adverse
climate/weather, overcrowding, poor sanitation, poor air quality and poor nutrition can
sometimes help living agents to cause disease in their host by weakening host resistance to
infection.
Food may get contaminated with physical, chemical and biological hazards during production,
harvesting, processing, distribution/transportation and storage. Food protection and control aims
at ensuring that food is not contaminated with the various hazards found in the environment. It
involves safeguarding food sources (e.g fishing environments), protecting food from
contamination with various food hazard (e.g by control of animal diseases, protective packaging
of food, hygienic transportation and storage), and elimination of biological hazards already in the
food through various means (e.g. heating, processing).
2 Environmental protection
Environment refers to the physical components of environment which include air, land and water
from which living organisms derive their livelihoods. Environment can also refer to the
combination of external physical conditions that affect and influence the growth, development
and survival of living organisms, or external conditions or surroundings especially in which
people live (living environment-housing and other basic facilities and needs), or work (work
environment -the conditions in which an individual or staff works including amenities). Living
environments focus primarily on living arrangements (and how they impact on health and
wellbeing of people. Environmental pollution can affect the survival and well-being of human
beings or living
organisms. Pollution is any addition of chemical or form of energy to air, water, soil or food that
threatens the health, survival or activities of human or living organisms. The environment can be
polluted by an array of contaminants including, hazardous (poisonous, toxic, irritant, or
corrosive) gases and particulates, poisonous chemicals (heavy metals, pesticides, herbicides etc),
disease causing microorganisms and radiation. The presence of these substances in the
environment constitute a health hazard to human beings. Prevention of environmental pollution
with these substances constitute environmental protection.
4 Environmental management
This is part of an integrated approach in the control of diseases. Environmental management
covers the following components:
1 Control of insect vectors and pests. Manipulation of environment to produce
temporary conditions that are unfavorable to vector or pathogen breeding, e.g. draining of
stagnant water, bush clearing around homes for control of mosquitoes. The permanent or long-
term modification of land, water and vegetation e.g clearing of vegetation, and or conversion of
forest to cropland will reduce occurrence of tsetse flies that transmit sleeping sickness
2 Provision of proper housing. The modification or manipulation of human habitation such
as proper construction of houses with adequate space, ventilation, lighting, sanitary facilities and
easy to clean to avoid accumulation of dirt are key to the control of diseases.
The aim of meat hygiene and inspection is to ensure the fitness and safety of meat for human
consumption. The meat and meat products should not carry pathogenic microorganisms that
might cause disease in man, nor shall they contain microbial toxins or chemical residues that
might affect public health. Adequate meat hygiene measures covers the time period animals are
on the farms before they are transported to the abattoirs, through its journey to and from the
abattoir until the consumption of the final meat products. It involves hygienic production of
food animals, ante mortem inspection of slaughter animals, handling and slaughter of animals,
post-mortem inspection of carcass, design and hygiene of slaughterhouses and meat industries,
design and hygiene of meat carriers, hygiene of slaughterhouse and meat industry personnel and
equipment, management of waste from slaughterhouses and meat industries.
Slaughterhouse staff (food handlers) should be made fully aware of and conversant with the need
for occupational hygiene, not only to avoid infection and food contamination but also from
becoming carriers of infection to their families and communities. Education on good standards of
health and hygiene and its importance are essential. Staff should be educated on:
Hygienic slaughter e.g using only properly washed and sterilized equipments e.g knives,
pangas, axes, saws.
Maintaining cleanliness of the personnel, operation area and the environment.
Operations and equipments in use in the slaughterhouse
How and why unsanitary practices should be avoided.
Thorough washing of hands with soap after visiting toilet, smoking, coughing or
sneezing, handling of money, garbage or soiled or infected material.
Knowledge that negligence may be detrimental to the whole community.
Personnel should be avoid the following unsanitary practices (or bad habits)
Walking aimlessly while operations are going on
Unguarded coughing and sneezing can spread pathogenic and respiratory bacteria:
Spitting on hands to enable firm gripping of an axe, panga e.g. while splitting
carcass
Licking of fingers to pick up items e.g. papers, utensils, paper towels or wrapping papers
or to turn over the pages of a book
Biting of finger nails or or cutting by using slaughter or meat inspection knife
Placing the pen, fingers in the mouth
Shaking of hands in the slaughterhouse
Smoking of cigarettes, hang or sniffing of tobacco
Unnecessary touching of meat
Chewing sweet-gum
Blowing or wiping of nose using bare hands or protective clothing
Scratching of head, use of mobile telephones
Eating, brushing of teeth, chewing miraa, Urinating near food
Taking beer/drunk ardness during operations
Changing of clothes near food/meat
Confrontations, fighting, playing in the slaughterhouse
Stealing of meat
Information Sheet 3- Operating and using equipment and materials assist the
community in understanding the VPH activities
Introduction
Raising awareness about various aspect of public health problems related to food of animal
origin at the community level is key for successful implementation of VPH program. Public
awareness includes: creating awareness on the risks associated with bad eating habits; risks of
drug residues in food and importance of meat inspection. A Community Awareness and
Participation Plan (CAPP) has been prepared to ensure widespread, ongoing, and meaningful
participation of the key stakeholders with a focus on the poor and the vulnerable groups. The
“reach” and “sustainability” of development initiatives are improved when all stakeholders,
including the poor and the vulnerable groups, have an opportunity to participate in shaping
investment programs and the voice of the poor is heard at all levels of decision- making.
Stakeholder analysis was undertaken to ensure that relevant stakeholders are identified and
included in the participatory project design process.
Social media has been an enabler of information dissemination, collaboration and coordination
for reasons ranging from personal to political. In healthcare information dissemination is a key
mechanism of creating awareness, a crucial factor in the early detection and prevention of
diseases. The social computing phenomenon (e.g. the proliferation of social media tools such as
Facebook,
YouTube,
Myspace,
Instagram
Twitter) is creating a new reality in health care, bringing social media to the
forefront of health information generation and dissemination.
Patients are changing from consumers of Internet content to generators of information using
social media sites and Web 2.0 tools. The term health 2.0 or medicine 2.0 is thus now commonly
used. Social media provides a forum for reporting personal experiences, asking questions, and
receiving direct feedback for people living with a disease. Through social media, support groups
have found a new platform for organizing as patients and family caregivers share their
experiences, seek consultation online, and connect with others.
Objectives
Inform
Educate
Users • Support
Individuals Patients Caregivers Family members
ShareOrganizations Healthcare
Support Advocate
Raise Funds
Create awareness
To
To Outcomes
Prevention
Detection
Functions Treatment
Connect Care
Communicate
Collaborate
Figure 3. The users and functions of social media to create health awareness
Information Sheet 4- Addressing current public health issues
Introduction
There is an urgent strategic need for health promotion to engage with the international discourse
on ‘sustainability’. To date much of the discussion and policy development addressing
‘sustainable development’ has treated the economy, livelihoods, energy supplies, urban
infrastructure, food-producing ecosystems, wild-ernes conservation and convivial communal
living as if they were ends in themselves. The ultimate goal of sustainability is to ensure human
well-being, health and survival. If our way of living, of managing the natural environment and of
organizing economic and social relations between people, groups and cultures does not maintain
the flows of food and materials, freshwater supplies, environmental stability and other
prerequisites for health, then that is a non-sustainable state. The second fundamental threat to the
improvement and maintenance of population health is the recent advent of unprecedented global
environmental changes.
It is well recognized that the media plays an enormously influential role in public responses to
health issues. The mass media - print, television, radio and internet - has an unparalleled reach as
a communication mechanism. It has substantial power in setting agendas, that is, what we should
be concerned about and take action on, and framing issues, that is, how we should think about
them. Public health professionals have always been sensitive to the persuasive power of the mass
media. In fact public health has often had the challenging task of both using the media to
influence health practices while countering this same influence where it encourages unhealthy
choices. These issues are especially acute in a crisis, such as the current A (H1N1) 'swine'
influenza pandemic. On such occasions hitting the right pitch is crucial, and difficult. Health
communicators may need to advocate rapidly and effectively for the public adoption of basic
preventive measures, like handwashing, while such messages may be
displaced in a mass media dominated by discussion of technical interventions, such as thermal
scanners.
Current global public health issues
Emerging and re-emerging infectious diseases
Unprecedented global environmental changes.
Declining regional life expectancy
Global pandemic diseases
In addition to electronic and print media current public health issues shall be
addressed through the following methods
Advancing interdisciplinary and implementation research in health with the involvement
of federal, provincial and local government for contextually tailored evidence to inform
policy.
Continuing to promote health research networking and alliance among academia,
healthcare institutions, scholars and researchers, with different stakeholders in national
and international level.
Facilitating to enhance knowledge and skills to conduct health research by young
researchers and scientists by increasing capacity, building opportunities, and availability
of research grants especially for the provinces.
Strengthening the mechanism to promote maximum use of digital technologies in
research process including dissemination.
Strengthening translation of locally generated evidence into policies and practices
through closer collaboration with policymakers.
Promoting responsible conduct of health research in academic and non- academic
institutions.
• Promoting health research in COVID-19 pandemic as per national guideline for
strengthening evidence generation on COVID-19.
LG 61
LO 2. Prepare to Conduct the Ante Mortem and Post-Mortem Examination
Information Sheet 1- Obtaining and preparing correct clothing, equipment and
resources for use
Introduction
Workplaces can be dangerous; there are many hazards that have the potential to kill, injure or
cause ill health or disease. Protecting the health and safety of people in the workplace is a
community expectation that makes good business sense. Workplace incidents can have a
dramatic impact on people’s lives (people in the workplace, families and friends), and they can
have significant financial impacts on organizations through loss of skilled staff and lost
production of goods or services.
A safe and healthy workplace and compliance with the law does not happen by chance or
guesswork. Good health and safety is all about eliminating and controlling hazards and risks.
This is best achieved by a proper consideration of the sources of harm and what can be done to
prevent the harm from occurring.
There are four important concepts that need to be understood so that this guide makes sense:
A hazard is something currently in, or may in future be in, the work environment that
has the potential to cause harm to people.
A risk is the chance (or likelihood) that a hazard will cause harm to people.
Harm is death, injury, illness (including psychological illness) or disease that may be
suffered by a person from a hazard or risk.
A control is a thing, work process or system of work that eliminates an OHS hazard
or risk or, if this is not reasonably practicable, reduces the risk so far as reasonably practicable.
Risk analysis comprises risk assessment, risk management and risk communication; these
terms are briefly defined below.
Risk assessment typically comprises hazard identification, exposure assessment, and
dose response assessment and risk characterization.
Risk management seeks to identify various options for mitigating risk and for selecting the
optimal course of action, after considering the benefits and costs, in consultation with interested
parties in industry, the government and academia, as well as the general public.
Risk communication is the process of consultation, discussion and review that seeks to
enhance the validity, effectiveness and general acceptance of risk assessment and risk
management.
Steps in controlling OHS hazards and risks
• CONSULTATION • CONSULTATION
STEP 1
STEP 2
Identify Hazards
Know what hazards Assess risk
are present Understand the nature
of risks, the harm that
could occur and the
likelihood
STEP 4
STEP 3
Check Controls
Control hazards
Review the
implemented controls and risks
to ensure they are Determine optionsfor
working as planned eliminating or reducing risk,
and effective selecting the best and
implementing it • CONSULTATION
• CONSULTATION
3.1. Introduction
Traceability is tracking the movement of identifiable products through the marketing chain.
Traceability can be used to convey information about a product, such as what it contains, how it
was produced and every place it has been. It is the marking of individual animals or group of
animals so that they can be tracked from place of birth/origin to slaughter. Producers keep
records on identities of each animal. A well designed traceability system provides accurate data
on the origin, sex, age, breed, movements, and records of treatments that an animal received.
Animal health information recording refers to the process by which indicators of the health
status of animal populations and related data on prevention, surveillance and outbreak
management are systematically collected, recorded, calculated and securely stored and made
accessible to users as appropriate.
Food safety: In slaughterhouses, meat traceability requires the clear identification of the live
animals through to meat products. A rigorous animal identification and trace back can prevent
potentially serious food safety problems. Traceability system can facilitate recalls of meat
products, enhance process controls, testing and other science based food safety measures. In
addition, it will possibly facilitate identification of the source of the disease, timely application of
control measures to contain its spread, as well as help authorities prevent future disease incidents.
Improved traceability may enable firms to limit their legal and financial liabilities.
Definition of terms
The concepts of sanitation, hygiene, cleaning and disinfection are very broad and to a
considerable extent overlap with each other, so for the purpose of this chapter we will assume
that:
Sanitation refers to all the processes and principles which are applied to ensure
that the microorganism count is kept at a safe low level in accordance with (official
health) regulations.
Hygienic refers to a condition that includes the concepts of “clean” and “safe” (in
other words the absence of harmful organisms or substances).
Cleaning refers to the ongoing process of cleaning which takes place throughout the day
and reaches its peak after the slaughtering process has ended. This process includes the
mechanical and chemical methods by which macroscopic, visible dirt is removed. When
an object appears to be clean, it is not necessarily free of harmful micro-organisms.
Disinfection refers to the process of removing by which micro-organisms and their
spores are killed or inactivated so that they cannot spread to other objects and
contaminate them.
Make work area clean
The aim of food hygiene is to ensure clean, safe and wholesome food.
It is extremely important for the management of an abattoir to be fully informed of their duties in
respect of hygiene, which the Act imposes on them. If they are not, a tendency could arise to
favour production above hygiene, or even attempt to economize on cleaning and disinfecting
materials.
1) All parts of an abattoir as well as fixed articles, equipment, tables and implements must
be kept clean and in good condition to the satisfaction of the meat inspector.
2) All parts of the abattoir, as well as all partitions, equipment and utensils used in the
abattoir and which come into contact with the carcass, meat or animal product, must be
thoroughly cleaned and disinfected at the end of the working day, or more frequently
should it be required.
3) All equipment, implements, tables, containers, disposal chutes and so on must be made
of a material that can be easily cleaned and sterilized.
4) All machinery and equipment used in an abattoir must be so designed and situated as to
be easily accessible for cleaning.
5) Equipment such as fillers, boilers, autoclaves, digesters and mixer tanks must, when not
in use, be kept at a temperature that inhibits the growth of heat resistant micro-
organisms.
6) All equipment that has been in contact with bile, faecal or disease-infected material must
be cleaned and sterilized immediately before re-use.
7) Metal brushes or steel wool may not be used, because they damage the surface of the
equipment; this makes proper cleaning and disinfection difficult.
8) Cloths may not be used for drying, as this only spreads contamination.
9) No polish or other substance that contains any poison may be used for the cleaning or
polishing of equipment. All such substances must be SABS approved.
10) After cleaning all utensils and surfaces of equipment, the abattoir must be thoroughly
disinfected, including the floors and walls.
11) The disinfection of an abattoir and its equipment, which is infected by a contagious
human or animal disease, must be done in a way and with a disinfectant approved by the
National Executive Officer.
12) The holding area must also be thoroughly cleaned and when necessary disinfected.
13) A water supply of at least 900 liters per slaughter unit in the case of red meat abattoirs
and 15 liters in the case of a chicken abattoir must be available to protect against
contamination, and the quality of this water must meet certain requirements.
14) A satisfactory supply of hot water at a minimum temperature of 40 - 50°C must be
available at all times during working hours where necessary for cleaning.
15) It is the responsibility of the abattoir owner to ensure that the premises are kept as free as
possible from rodents, birds, cats, dogs, flies and other insects at all times, and that no
breeding place or circumstances are permitted on the premises which could encourage
the breeding of vermin.
Information Sheet 5- Performing ante mortem examination of animal
Introduction
Ante-mortem inspection identifies animals not fit for human consumption. Here animals that are
down, disabled, diseased, or dead are removed from the food chain and labeled “condemned.”
Other animals showing signs of being sick are labeled “suspect” and are segregated from healthy
animals for more thorough inspection during processing procedures. Animals or birds are often
transported directly from the farm to slaughterhouse. In this process, vets have many different
roles. Initially vets may be involved in checking the health and welfare of the animals at some of
the stages of transport to the slaughterhouse and once there, in the ante-mortem inspection prior
to slaughter.
Definition of terms
Abattoirs - The word abattoir comes from French word ‘‘abatte’’ which is to mean slaughter,
and thus abattoir is defined as any premises or an establishment where approved and registered
by the controlling authority for the slaughtering of specific animals for human consumption. It is
specific since every species of animals has its requirement. Abattoir or slaughter houses may be
owned publically (E.g. Municipal abattoir, state abattoir). It can also be export abattoir or local
abattoir.
Lairage It is an area where animals are kept prior to slaughter. The floor should be non- slippery
concert floor which should be washed daily in order to minimize surface contamination. Instead
of concrete if it is cement, muddy & moist it can contaminate the carcass and is comfortable for
the multiplication of bacteria. Animals in lairage pens should not be kept for more than 72hrs
because of the risk of cross infection and surface contamination with some pathogenic bacteria
such as Salmonella, which are frequently excreted by some animals. The lairage should be
divided in to pens where different group of animals can be accommodated. The animals arriving
at slaughter house from different sources should be separated in order to avoid fighting; the
lairage
must be large enough to accommodate 3 days’ supply of stock and must have direct access to the
stunning room.
Cleaning means the removal of objectionable matter.
Inspector means a properly trained officer appointed by the controlling authority for the
purpose of meat inspection and control of hygiene, and includes a veterinary inspector. The
supervision of meat hygiene, including the inspection of meat, should be under the responsibility
of a veterinary inspector.
Slaughter animal means any animal lawfully brought into an abattoir for slaughter.
Inspection refers to examination of meat and other products for abnormalities and diseases.
Meat hygiene may be defined as an expert supervision of meat and meat products with the
objectives of providing whole some meat for human consumption by excluding all factors that
endanger the public health.
A proper inspection service consists of a veterinary examination of the live animal (ante- mortem
inspection), and examination of the carcass and offal (post-mortem inspection) and animal
products, where necessary, laboratory tests of body tissues and fluids. Meat is defined as flesh of
animals used as food. The word meat includes both skeletal muscles and visceral organs or edible
offal such as kidney, liver, heart, tongue and other visceral organs or offal accepted and used as
food. Thus the definition of meat depends on the culture and eating habit of individual society or
country.
General examination:
No animal shall be slaughtered without undergoing AM inspection
AM Inspection started within 24hrs of delivery
If 24hrs elapsed after AM, the inspection should be repeated,
Should be made in well-lighted lairage pen and crush
AM should be started by
Checking for movement permit before animals are offloaded and
Do not have dirty hide, skin or fleece conditions which cause ri sk of contamination
Properly identified, accompanied by the relevant information from the holding/fattening areas.
Sufficiently rested, Healthy, satisfactory state as regards to welfare. Collecting and recording
disease and management history of the animals
General Ante-mortem examination done properly and systematically in two stages.
Observation of animals both collectively and individually when at rest
Certain abnormal signs such as labored breathing are easier to detect while the animals
are at rest;
Observation of animals both collectively and individually at motion.
Whilst other abnormalities, such as lameness, may not be detected until the animals in
motion.
Figure 7. A government vet performing ante-mortem inspection on live pigs Record the
detail information and parameter on ante-mortem inspection card
Table 2 Ante mortem inspection record sheet
Introduction
Ante-mortem examination of meat animals is of prime importance and the initial step in
detection of any sign of disease, distress, injury etc. which helps in taking appropriate decision
before slaughter of animal. Ante-mortem inspection should commence by observing animals
when they are unloaded. At this time, a declaration of health and origin must be provided for all
animals by the owner of the slaughter stock and recorded by the abattoir owner and such health
declaration must contain the following information
– date of delivery; name and address of owner or farm; number of animals and specie(s); health
status of the herd(s) including mortality rate; and medication, if given as well as withdrawal
periods and dates.
On the day of arrival at the abattoir animal must be inspected properly and systematically under
adequate natural or artificial lighting at the lairages pens, and the inspection must be repeated on
the day of slaughter if the slaughter is not done within 24 hours of arrival. Only animals that are
judged to be sufficiently rested should proceed to slaughter, but should not be withheld from
slaughter any longer than necessary. An animal may have dirty hide, skin or fleece, if it is
suspected that a forbidden substance has been administered to it or an animal which is on the
point of giving birth or which has given birth in transit or lairage may not be slaughtered;
Table 3 Inspection of live animals, sign of health and sickness
No Signs of health Signs of sickness
Introduction
Ante mortem inspection is the inspection of live animals to ascertain their health condition
and fitness for slaughter. Ante mortem inspection takes place at the farm, as animals enter
the slaughterhouse and when in the lairage just before slaughter. Ante mortem inspection
should be carried out in adequate lighting where the animals can be observed both
collectively and individually at rest and motion. The general behavior of animals should be
observed, as well as their nutritional status, cleanliness, signs of diseases and abnormalities
should be also observed..
Introduction
The following factors should be considered for animals that are going to be slaughtered for
human consumption:
The fatigued and exited animals must be given rest before slaughter. A period of
12 to 24 hours with a maximum of 36 hours detention and rest in a lairage is essential. The
duration of resting period depends on species of the animals, age, sex, condition, time of the
year, length of the journey and method of transportation.
Animals should receive ample potable drinking water during their detention in the lairage.
This helps to lower the bacterial load in the intestine and facilitates removal of the hide
during dressing of the carcass.
Withholding food from cattle for a period of 6 hours prior to slaughter minimizes the
migration of bacteria from the intestinal tract during digestion. Moreover fastened animals
bleed better, carcass is easier to dress and has a brighter appearance.
Regular cleaning and disinfection of a lairage to lower the risk of cross contamination.
Animals should be kept in well ventilated, dry and hygienic lairage.
The animals which are badly soiled, dirty and doggy should be properly washed.
The lairage should be 10m away from slaughter house. Lairage areas for each specific
abattoir should therefore be assessed relative to expected throughput. Pen areas required for
each species are for cattle 1.7m2/head, for pigs/sheep 0.35m2/head and for goats’
0.25m2/head.
Sufficient space should be provided to each animal.
Aggressive animals should be isolated in lairage.
Females in oestrus should not be kept with males in lairage.
Design of lairage pen should allow free movement of stock.
Stock should be kept in their original social groups as far as possible and there should
be no mixing within the last 24-48 hours before slaughter.
Avoid pre-slaughter stress to prevent dark, firm and dry meat.
Information Sheet 10- Carrying out humane methods of
slaughtering
This is the process of putting the animal to death. It involves, stunning, bleeding, flaying,
evisceration, carcass splitting (cattle/large stock), trimming and washing, chilling and freezing.
Page 45 of 185 Holeta PTC TVET program title- Animal Health Care Service Version -1
Author/Copyright Level - 4 September, 2021
The acceptable methods of stunning
Sledge hammer: it contains a long handle. It is made up of iron. The weight of hammer is
around 2 kg. It is used for striking an animal on the forehead and within few minutes the animal
becomes unconscious.
Captive bolts: Stunning by concussion of the brain through an impact of the bolt with
the skull of animals. Gun powder (cartridge), compressed air and spring under tension drive bolts
through the skull of animals. This type of stunning is widely used for all farmed animals. It is
called ‘captive’ since the bolt is shot out of the barrel but remains attached to the pistol. A
captive bolt stunning gun kills the animal and reduces it instantly unconscious without causing
pain. A captive bolt gun has a steel bolt that is powered by either compressed air or a blank
cartridge. The bolt is driven into the animal's brain. It has the same effect on the animal as a
firearm with a live bullet. After the animal is shot the bolt retracts and is reset for the next
animal. A captive bolt gun is safer than a firearm. There have been some questions about
whether or not a captive bolt actually kills an animal. Practical experience in slaughter plants
indicates that cattle shot correctly with a penetrating captive bolt have irreversible damage to
their brain and they will not revive. If a no penetrating captive bolt is used the animal may revive
unless it is bled promptly.
Electrical: here we use the electrical current of magnitude of 75 volts for 10 seconds. The
technique involves the application of a pair of electric tongs on either side of the animal’s head.
An electric current is then passed through the brain and this supposedly leads to the temporary
loss of consciousness.
Firearm (gunshot): Used for animals difficult to handle such as wild pigs, bison, deer,
horses or in emergencies.
Carbon dioxide: A CO2 gas chamber is designed on the principle that carbon dioxide is
heavier than normal atmospheric air. Birds may be stunned using CO2 and Argon gas while they
remain in their crates. Pigs are also gassed using CO2. Pig becomes unconscious by 70% CO2
within 20-25 seconds.
Bleeding: Bleeding is effected by severing the neck at the level of the jugular and hanging
the animal upside down on the rails
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Flaying is the removal of the hides and skin. It should be done carefully to preserve the
wholeness of the hides and skins. They should not be punctured during flaying. Intact hides and
skin fetch high prices as they produce quality leather and leather products. Proper flaying is done
using special knives or pneumatic flaying machines. These yield hides and skins with minimum
puncturing. The major sources of microbial contamination of carcasses is faeces. Faeces and soil
adhering to the animal is carried to the abattoir on the hair, hide and hooves and tail of the
animal. Efforts should be made to wash and dry the animals before they enter the plant. In
addition, tails need be bagged/covered until hide is removed which is then kept separate from
carcasses.
Evisceration: is the removal of internal organs such as stomach, liver, spleen,
intestines and lungs. Should be done carefully to avoid puncturing of the stomach and spillage of
its contents which can contaminate the carcass. Stomach contents contain a lot of disease agents
that cause food poisoning to consumers.
Carcass splitting: Carcasses are split carefully through the backbone into two equal portions.
The splitting can be accomplished manually using a panga and or an axe or by use of an
electrical power saw.
Washing: After evisceration, the carcass is washed to remove any surface contamination with
blood and intestinal contents. It is important to use potable water to wash carcasses as any other
water can introduce pathogens on to the carcass. Both hot and cold water should be provided in
the slaughterhouse. Carcasses should not be wiped with any type of cloth.
Trimming: This is meant to remove contaminated and unaesthetic parts of the carcass. The aim
is to enhance the aesthetic value of the carcass.
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The general slaughtering process animals
Carcass chilling
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Self-Check 10– Written Test
Name ID Date
Directions: Answer all the questions listed below. Examples may be necessary to
aid some explanations/answers.
Test I. Write true if the statement is correct/False if it is incorrect for the following
questions (6 point each).
1. Stunning is a technical process that induces immediate unconsciousness and
insensibility in animals.
2. Carcass splitting is the removal of internal organs such as stomach, liver, spleen,
intestines and lungs.
3. Bleeding is effected by severing the neck at the level of the jugular and hanging the animal
upside own on the rails.
Test II. Choose the best answer for the following questions (6 point).
1. meant to remove contaminated and unaesthetic parts of the carcass.
A. Trimming
B. Evisceration
C. Flaying
D. Stunning
Test III. Short Answer Questions (10 point)
1. What are the objectives of stunning animal?
2. Write the precautions and the general principles that apply to all stunning methods
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Operation Sheet: 1. Procedure of Ante mortem Inspection
Procedure for ante mortem inspection of animal Objective:
2. Animals should be inspected in a standing position and in motion, and while they are not
excited. Observed abnormalities and signs of disease; species and behaviour of animals; in
sick or suspected diseased animals, notice should be taken of age, colour and specific
markings; Cleanliness of slaughter animals.
3. Particular attention should be paid to the following details: manner of standing and
movement; state of nutrition; reaction to environment; condition of hide, skin, hair and
wool; temperature, respiratory…
4. No animal which has been received into a slaughter hall for the purpose of being slaughtered
shall be removed from the slaughter hall before being slaughtered except with the written
consent of the Qualified Veterinary Doctor.
5. An animal declared as “suspect” on ante-mortem inspection but which does not plainly
show any disease or condition that would cause its entire carcass to be condemned shall
maintain its identity as “suspect” until its carcass and all organs are finally inspected by the
Qualified Veterinary Doctor.
6. No animal in a febrile condition shall be permitted for slaughter.
7. Animals presented for slaughter and found in a dying condition on the premises of a factory
due to recent disease shall be marked as “condemned” and disposed of as provided for
“condemned” animals.
8. Every animal which, upon examination, is found to show symptoms of or is suspected of
being diseased or animals declared as “suspect” shall at once be removed for treatment to
such special pen and kept there for observation for such period as may be considered
necessary to ascertain whether the animal is diseased or not.
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9. Where signs indicate a systemic involvement, a communicable disease, or toxicity from
chemical or biological agents which do or may render the meat unsound, the animal so
affected should be withheld from slaughter and condemned forthwith as unfit for human
consumption
10. Animals' exhibiting normal behaviour but known to have been treated or given drugs and to
be carrying consequent residues or which are carrying residues from other sources, should
be either condemned or withheld from slaughter until the residues are excreted
11. During the inspection, record should be taken
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LAP TEST
Name ID Date
Instructions: Given necessary templates, tools and materials you are required to
perform the following tasks within 1 hour. The project is expected from each
student to do it.
During your work: You can ask all the necessary tools and equipment
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LG 62
LO 3. Carry out Post-mortem Examination
Instruction sheet
This learning guide is developed to provide you the necessary information regarding the
following content coverage and topics:
Ensuring complete bleeding prior to dissection
Conducting dissection using appropriate techniques and equipment
Inspecting organs, tissues and structures using post mortem inspection
procedures
Taking and preparing samples of organs, tissues and fluid
Conducting inspection works on meat
Disposing unfit offal and waste products
This guide will also assist you to attain the learning outcomes stated in the cover page.
Specifically, upon completion of this learning guide, you will be able to:
Ensure complete bleeding prior to dissection
Conduct dissection using appropriate techniques and equipment
Inspect organs, tissues and structures using post mortem inspection procedures
Take and prepare samples of organs, tissues and fluid
Conduct inspection works on meat
Dispose unfit offal and waste products
Learning Instructions:
1. Read the specific objectives of this Learning Guide.
2. Follow the instructions described below.
3. Read the information written in the “Information Sheets”. Try to understand what are being
discussed. Ask your trainer for assistance if you have hard time understanding them.
4. Accomplish the “Self-checks” which are placed following all information sheets.
5. Ask from your trainer the key to correction (key answers) or you can request your trainer to
correct your work. (You are to get the key answer only after you finished answering the
Self-checks).
6. If you earned a satisfactory evaluation proceed to “Operation sheets
7. 7. Perform “the Learning activity performance test” which is placed following
“Operation sheets” ,
8. If your performance is satisfactory proceed to the next learning guide,
9. If your performance is unsatisfactory, see your trainer for further instructions.
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Information Sheet 1- Ensuring complete bleeding prior to dissection
Definition of terms
Slaughter Animal: means animals intended for human consumption (Cattle, sheep, goat,
swine, camel, and poultry)
Bleeding: - The main purpose of bleeding is to remove blood from the carcass
otherwise it cause decomposition since blood is a good medial for bacterial growth and
multiplication. In general bleeding of animal throughout the world varies depending on
Hauling: hanging the animal down ward .the animal bleed well when the animal is
hanging downward rather than when bleed in horizontal position .this is due to the low of
gravity.
Slaughter: killing of an animal by severing the major blood vessels in the neck region, with
or without stunning.
Stunning: is the process of making the animal unconscious. It is mostly practiced in most
of the modern slaughter houses.
The purpose of stunning is to
Reduce stress on the animal
To prevent injury to the butcher
To promote efficient bleeding
To make unconscious the animal
Bleeding:
Bleeding is effected by severing the neck at the level of the jugular and hanging the animal
upside down on the rails. Bleeding is hastened by hoisting and hanging the animal upside down
on the rails for some time. It is expected that as much blood as possible (up to ½ of total amount)
is removed from the stunned animal after the bleeding process. It is important that the heart
continues beating after stunning to ensure complete bleeding.
The efficiency of bleeding has a bearing to the keeping quality of meat. Blood is a good medium
for growth of microorganisms and hence its presence in the carcass will accelerate (hasten)
spoilage/deterioration of meat. A carcass that is not well bled looks
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dark and muscles ooze (produces) blood when cut. Blood vessels (especially subcutaneous and
intercostal vessels appear injected with blood while visceral organs (lungs, heart, liver and lymph
nodes) contain excessive blood, are fluffy and watery. Carcasses that are not properly bled are
condemned during meat inspection.
The animal is hanging in a bleeding area, perhaps over a trough. At least 6-8 minutes must be
allowed for bleeding cattle, 5-6 minutes for pigs and 3-4 minutes for sheep. If shorter periods are
allowed, blood will drip on the dressing floor, causing contamination in this work area or a loss
of blood meal where by-products are manufactured. The bleeding trough floor must slope steeply
in the direction of a floor drainage opening situated directly below the bleeding point. It is
advisable to drain the blood separately, so there should be a second opening for washing water.
The opening, which is not in use, must be closed with a plug. There are still problems attached to
the disposal of blood other than by processing in a sterilization plant. If blood is disposed of in
the drainage system it overloads the purification works, while unpleasant odours emanate from
septic tanks into which it is drained. Larger abattoirs in particular experience problems with the
burying of blood.
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Reduces tedious work
Save space
Blood do not remains in the carcass so decomposition is not faster
Avoid contamination
Sometimes, knife reaches too far in the chest puncturing the pleura and blood may be aspirated
into the thoracic cavity. This results blood adheres to the parietal pleura especially the posterior
edges of the ribs. This contamination of lungs is called back bleeding or overstocking.
Table 4. The minimum time allowed for bleeding and the amount of blood per species are:
Cattle 6 minutes 13 – 15 litres blood
Calf 5 minutes 2 – 7 litres blood
Sheep 5 minutes 1,3 – 2 litres blood
Pig 6 minutes 2 – 4 litres blood
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Self-Check 1 – Written Test
Name ID Date
Directions: Answer all the questions listed below. Examples may be necessary to
aid some explanations/answers.
Test I. Write true if the statement is correct/False if it is incorrect for the following
questions (4 point each).
1. Slaughter is killing of an animal by severing the major blood vessels in the neck
region, with or without stunning.
2. Horizontal bleeding is more advantagious the vertical bleeding.
3. . At least 5-6 minutes must be allowed for bleeding of pigs carcass.
Test II. Choose the best answer for the following questions (4 point).
1. What is the minimum time allowed for bleeding of bovine carcass?
A. 6 minutes
B. 5 minutes
C. 5 minutes
D. 7 minutes
Test III. Short Answer Questions (6 point)
1. Describe the purpose of bleeding.
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Information Sheet 2- Conducting dissection using appropriate techniques and
equipment
Introduction
Dissection: is the process of cutting into a dead animal body apart or separating tissues as, for
example, in the study of anatomy or in the course of a surgical procedure. In the case of study
dissection has its own importance. Helps student learn about the internal structures of animals.
Helps the trainees learn how the tissues and organs interrelated. Gives the trainees an
appreciation of the complexity of organisms in a hands on learning environment.
The splitting of the carcass is an exacting task as the two halves must be of equal size and
weight for trading purposes, economical cuts and easy handling of the carcass. Band saws are
most frequently used. The operator is positioned behind the carcass and starts sawing by placing
the blade on the vertebra which is visible between the hind legs. The blade guides should be
pressed against the carcass surface while sawing to prevent the blade from bending. Hot water at
82 °C must be available for sterilizing the saw, especially after contamination.
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The present development relates to a kind of beef carcass auto-splitting machine, half device and
cohesion device and jacking system in work box are split including the work box with base, on
work box, work box includes holding room, drive chamber and Transmission Room tightly,
Transmission Room is positioned at the top for holding room and drive chamber tightly, in the
outside for holding room tightly, the support frame with guide rail is set, the location-plate of two
pieces of circular arc types is provided with the outside of support frame, support frame and
location-plate are fixedly connected between the extending part of work roof box and the
extending part of base plate. C-type holding arm of the present invention can hold beef carcass
tightly, beef carcass is set to be fitted on two pieces of circular arc type location-plates, so
that in gap of the backbone between two pieces of circular arc type location-plates, saw blade
carries out splitting half to beef carcass from the bottom to top under jacking system drive along
guide rail, it ensure that and split precision partly, reduce bone and flesh waste, improve the
operating efficiency for splitting half operation, ensure that the appearance for splitting half
section.
Carcass splitting machine: Which main used to cutting cattle carcass into half carcass for
chilling and other processing.
Operation instruction
The user have to check motor oil is enough and blade moving direction is stable
When the cattle carcass arrived in position then
Put slitting saw between two hinds legs then turn on working with meanwhile the staffs
have to keep pressure the saw and cutting carcass through back side of carcass
When splitting saw working the user will open cleaning valve of saw to cleaning oil and
meat on saw blade in order to make saw working smooth
Meets national and international requirements for hygiene and safety
2. Trimming of Carcasses: After inspection and before the final washing, all approved
carcasses can be finally trimmed to remove contaminated and unaesthetic parts of the carcass.
The aim of trimming is to enhance the aesthetic value of the carcass. The following removed
or cut off:
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Spinal cord
Left-over bits of skin and intestines
Portions of male and female genitals
Bloody membranes on the inside of the neck, and the aorta
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Self-Check 2 – Written Test
Name ID Date
Directions: Answer all the questions listed below. Examples may be necessary to
aid some explanations/answers.
Test I. Write true if the statement is correct/False if it is incorrect for the following
questions (5 point each).
1. Carcass splitting machine is main used to cutting cattle carcass into half carcass for chilling
and other processing.
2. After inspection and before the final washing, all approved carcasses can be finally
trimmed to remove contaminated and unaesthetic parts of the carcass.
Test II. Choose the best answer for the following questions (6 point).
1. Which one of the following is not the importance of animal dissection?
A. Helps student learn about the internal structures of animals.
B. Helps the trainees learn how the tissues and organs interrelated.
C. Gives the trainees an appreciation of the complexity of organisms in a hands on
learning environment.
D. None of the above
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Information Sheet 3- Inspecting organs, tissues and structures using post
mortem inspection procedures
Introduction
Post-mortem inspections refers to examination of the carcass and associated organs after
slaughtering to detect and eliminate abnormalities including contamination to ensure only meat
fit for human consumption is passed for food. Post-mortem meat Inspection is a systematic
process of examination of the dressed carcass, pluck (liver, heart, lungs and lymph nodes), and
head aimed at identification of known and unknown diseases or conditions. Routine post-
mortem inspection should be carried out as soon as possible after carcass dressing is completed.
Beef carcasses set rapidly and if inspection is delayed particularly in cold weather, the
examination of the carcass becomes more difficult. Post-mortem inspection aims to protect
consumers against zoonoses and unwholesome meat. The reasons for post-mortem inspection
are to:
Check the efficiency of slaughter and carcass dressing techniques
Identify diseased carcasses and organs and remove them from the food chain
Detect visible contamination to avoid harmful and unaesthetic products from reaching the
consumer
Ensure hygienic processing of meat
Definition of terminologies
Carcass: Dressed body of animal (cattle, buffalo, sheep, goat, pig or poultry).
Dressing: separation of the body of an animal in to a carcass and other edible and inedible
parts.
Edible organs include tongue, heart, liver, kidney and part of intestine. Inedible organs are
spleen.
Condemned: In relation to a slaughter animal or meat, means inspected and judged as, or
otherwise officially determined to be, unfit for human consumption and requiring destruction.
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Total condemnation: Means the entire carcass and offal are condemned (Judgement symbol
T).
Partial condemnation: Means only parts of the slaughtered animal are condemned,
while others are judged otherwise (Judgement symbol D for the condemned diseased or defective
parts).
Contamination: Means objectionable matter, and includes substances and/or
microorganisms that make fresh meat unsafe and/or unwholesome.
Controlling authority: Means the official authority charged by the government with the
control of meat hygiene, including meat inspection.
Disease or defect: Means a pathological change or other abnormality.
Disinfection: of plant, facilities and equipment, means the reduction, without adversely
affecting the meat, by means of hygienically satisfactory chemical agents and/or physical
methods, of the number of microorganisms to a minimum.
Dressing: means the progressive separation on the dressing floor of a slaughter animal into a
carcass (or sides of a carcass), offal and inedible by-products and may include the removal of the
head. Examples of dressing include the removal of the head, hide or skin, genital organs, urinary
bladder, feet, and in lactating animals, the removal of the udder.
Edible offal: in relation to slaughtered animals means offal that have been passed as fit for
human consumption.
Fit for human consumption: in relation to meat means meat that has been passed by an
inspector as safe and wholesome, unless found unwholesome in subsequent examinations, which
may include laboratory tests.
Fresh meat: Means meat that has not yet been treated in any way other than by modified
atmosphere packaging or vacuum packaging to ensure its preservation, except that if it has been
subjected only to refrigeration, it continues to be considered fresh for the purposes of this Code.
Inedible: Means inspected and judged to be, or otherwise officially determined to be,
unfit for human consumption but not requiring destruction.
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Contaminant: Means any substance not intentionally added to food, which is present in such
food as a result of the production (including operations carried out in crop husbandry, animal
husbandry and veterinary medicine), manufacture, processing, preparation, treatment, packing,
packaging, transport or holding of such food or as a result of environmental contamination. The
term does not include insect fragments, rodent hairs and other extraneous matter.
Safe and wholesome: Refers to meat that has been passed as fit for human consumption
using the criteria that it:
Will not cause food borne infection or intoxication when properly handled and
prepared with respect to the intended use;
Does not contain residues in excess of established Codex limits;
Is free of obvious contamination;
Is free of defects that are generally recognized as objectionable to consumers;
Has been produced under adequate hygiene control; and
Has not been treated with illegal substances as specified in relevant national
legislation.
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No removal of organs or carcass before final judgment is passed
No removal of identification marks
General approach:
Routine postmortem examination of a carcass should be carried out as soon as
possible after the completion of dressing in order to detect any abnormalities so that
products only conditionally fit for human consumption are not passed as food.
There are three cardinal principles used in meat inspection. They include:
Visual examination of animal carcass and organs: This is important for purposes
of animal identification (species, sex, age), to determine the nutritional state of the animal
and state of bleeding, detect bruises, deformities, edema, peritonitis, discolorations and
general contamination. Examination of gums reveal vesicles or erosions arising from FMD
rinderpest and mucosal disease. Examination of lungs may reveal pneumonia, abscesses
and TB lesions. Palpation: Identify abscesses, abnormal growths (tumors), deformities,
abnormal consistency of fat and various body organs, hydatid cysts and TB lesions in the
lungs.
Incisions: These are made with sharp knives to show clean undistorted surfaces.
Standard incision are made to identify Cysticercosis in beef and pork muscles (tongue,
masseters, triceps brachii), liver flukes in livers, aspiration pneumonia in lungs, abscesses
and congestion in lymph nodes
A systematic approach should be used to ensure that nothing is missed. In general for red
meat animals the systematic approach includes examination of the:
Structures of the head;
Organs (viscera) in the thoracic (chest) cavity;
Viscera in the abdomen;
Carcass
Special attention will be paid to examining the lymph nodes in all parts of the body: The
lymphatic system is a one way closed circulatory system that allows tissue fluids to be
returned to the blood stream. Any time there is an infection in an area,
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or region, of the body, the lymph nodes of that area, or region, will show evidence of the
infection. The degree of spread of an infection through the lymphatic system is a major
factor in determining what should, or shouldn’t be condemned
1) Head inspection procedure
Designated abattoir personnel should prepare the head in a manner that makes it suitable for
inspection. In general this preparation includes:
Immediately separate the head from the body, placed in the head holder
Skinning the head; and removing the ears, third eyelid, eyes, external ear; horns.
Cleaning and washing with potable water, with emphasis on oral and nasal cavity
the head;
Placing the head on the inspection rack with all lymph nodes in place and the
tongue still attached
Inspectors will:
Determine the age of the animal by reviewing reliable birth date documentation or by
examining the lower incisor teeth.
Conduct a visual examination of the inner and outer surfaces of the head.
The inspector will be looking for evidence of contamination as well as for nasal
discharges, ear mites, dental and cheek abnormalities and conditions of the eye.
Visually observe the head to ensure that it is free of any hair, hide, horns, ingesta or any
other type of contamination.
Common areas of contamination include the base of the skull, the area where the horns
were attached and inside the mouth.
The head should be held for trimming and re-inspection if there is any contamination or
other dressing defects.
The eyes should be closely examined for any evidence of cancer eye. Unless the cheek
meat is removed on the kill floor the tonsils should be removed under the supervision of
an Inspector.
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Incise (cut) the outer and inner masseter (cheek) muscles.
This is done primarily to check for Cysticercus bovis. C. bovis is the intermediate form of
the beef tapeworm which affects humans. Incision, of these muscles, will also
occasionally detect other conditions. The incisions should be parallel to the mandible
(jawbone) and they should go right through the muscles.
Observe and incise the following lymph nodes for any evidence of edema, enlargement,
abscesses, grittiness, or tumors.
a) Left and Right parotid;
b) submaxillary (mandibular);
c) Left and Right retropharyngeal
Visually examine, palpate and incise (if necessary) the tongue.
This is done to check for abscesses, Actinobacillosis (wooden tongue) and other
abnormalities. Localized conditions such as scars, sores and erosions are trimmed.
Upon completing the inspection of the head, the operator in charge of inspection removes the
tonsils and pharynx. (Inedible).
Epiglottis Tongue
Position of parotid lymph node
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attachment to the trachea and viewed. As part of inspection of all cattle and calves over the age
of 6 weeks for Cysticercus bovis, the muscles of mastication should be viewed and one or more
linear incisions made parallel to the lower jaw into the external and internal muscles of
mastication; in addition one incision into triceps brachii, 5 cm behind the elbow, should be
made.
Liver
Give visual inspection and thoroughly palpate entire surface (both sides)
View the gall bladder
The hepatic (Portal) lymph nodes should be examined and incised 2-3 times.
The bile ducts should be opened longitudinally to observe for liver flukes.
The liver shouldn’t be incised unless a deep lesion is palpated. For cattle over six weeks
of age, incise as deemed appropriate to detect liver flukes.
For sheep, pigs and game, incise as deemed appropriate for parasite.
Lymph nodes Portal (hepatic) view and incise.
Examine for fatty liver, faciolosis, actinobacillosis, Cysticercus bovis and certain
parasitic conditions.
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Heart
Examine the exterior and interior surfaces of the heart visually after the removal of the
pericardium, including the heart valves should be observed visually.
There are two options for incising the heart. The first is to make one or more incisions
from base to apex that passes through the wall between the left and right ventricles.
This allows observation of all of the heart valves. The second option is to evert the heart
and make 3 to 5 shallow cuts in the musculature. These incisions should extend all the
way through to the outer surface of the heart. If there is any suspicion that the animal may
be affected with Cysticercus bovis, more incisions can be made.
Lungs
Visually examine the exposed surfaces of the lungs then palpate them and incise (cut)
them if indicated. The lungs are palpated to detect any deep lesions such as abscesses,
tumors, chronic pneumonia, etc. If any lesions are detected, by palpation, the lungs
are incised.
Examine the left and right bronchial, cranial and caudal mediastinal lymph nodes.
These nodes should be incised 2 or 3 times.
Inspector makes a cross-section incision to the trachea thus exposing the main
bronchi then transverse vertical incision across the diaphragmatic lobes, main
bronchus each trying to inspect the secondary and tertiary bronchi.
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A Bronchial lymph node
Spleen:
Inspect visually and palpation
The surface and substance should be examined for anthrax, hematoma,
infarction and tuberculosis.
Gastro-intestinal tract
For a proper examination the intestines should be spread out.
1) Visually examine the four compartments of the stomach (reticulum, rumen, omasum and
abomasum). There may be evidence of localized peritonitis, or abscess formation, if the
reticulum has been penetrated by a nail, or piece of wire
2) View and incise mesenteric lymph nodes. Usually incision to the mesenteric lymph nodes
necessary if enlarged or if there is any possibility that the animal has tuberculosis.
3) View and incise if any lesions are observed in the submaxillary lymph nodes.
4) The omentum, mesentery and any other fatty tissue that is going to be used for human
consumption must be free of contamination
5) The serous surfaces of the organs are examined for tuberculosis and Actinobacillosis.
Reticulum should be assessed for foreign body.
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Mesenteric lymph nodes
3) Carcass inspection
Designated abattoir personnel will prepare
1. The carcass in a manner that makes it suitable for inspection. Proper presentation includes:
Removing the viscera (internal organs);
Splitting the carcass;
Presenting the carcass before final trimming and/or washing
2. A system will be in place to ensure that the carcass can be matched up with the head and
all internal organs until such time as all PM inspections have been
completed.
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This inspection includes the:
1) The registered inspector must inspect a carcass by means of observation, palpation,
smell and, where necessary, incision and must take into consideration
Its state of nutrition; its color; its odor; its symmetry;
The efficiency of its bleeding; condition of serous membranes (pleura and peritoneum)
Cleanliness and the presence of any contamination;
Its pathological conditions; any parasitic infestation;
Any injection marks; any bruising and injuries;
Any abnormalities of muscles, bones, tendons, joints or other tissues; and
The age and sex of the animal from which it was derived.
2) Conduct a visual inspection of the entire carcass. (Including Joints, musculature, exposed
bones, joints, tendon → Outer muscular surfaces, Diaphragm→Peritoneum
→Pleura→Neck).
3) Palpate and incise (if deemed necessary) any observed abnormalities.
4) Remove the entire spinal cord from all carcasses.
5) Identify all carcasses, or portions thereof, that are condemned so that they will be handled in
a manner that ensures that contamination of equipment, meat, or meat products, from other
carcasses, does not occur. No part of a condemned carcass can be used for human
consumption
6) The main carcass lymph nodes – being the precrural, popliteal, anal, superficial inguinal,
ischiatic, internal and external iliac, lumbar, renal, sternal, prepectoral, prescapular and
atlantal nodes, as well as the lymph nodes of the head and viscera
– should be incised and examined in all animals in which systemic or generalized disease is
suspected, positive to a diagnostic test for tuberculosis and in which lesions suggestive of
tuberculosis are found at post-mortem inspection. In all animals the following examination
techniques should be used for specific lymph nodes:
Superficial inguinal (male) – palpate;
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Supramammary (female) – palpate and incise when udder is or has been in
lactation, or in the case of mastitis;
Eexternal and internal iliac – palpate
Prepectoral – palpate;
Popliteal – palpate (only sheep/goats);
Renal – palpate (cattle) or incise if disease is suspected;
Prescapular and prefemoral – palpate (only sheep and goats).
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Self-Check 3 – Written Test
Name ID Date
Directions: Answer all the questions listed below. Examples may be necessary to
aid some explanations/answers.
Test I. Write true if the statement is correct/False if it is incorrect for the following
questions (5 point each).
1. Cndemnation is separation of the body of an animal in to a carcass and other edible and
inedible parts.
2. Inedible organs means unfit for human consumption but not requiring destruction.
Test II. Choose the best answer for the following questions (6 point).
1. Wich one of the following is not the principles of post-mortem inspection?
A. The slaughter hall and equipments are clean
B. The sewage system is functioning properly
C. The availability of adequate supply of potable water
D. Availability of adequate light
E. None of the above
2. A systematic approach of meat inspection includes examination of the:
A. Structures of the head;
B. Organs (viscera) in the thoracic (chest) cavity;
C. Viscera in the abdomen;
D. Carcass
E. All of the obove
Test III. Short Answer Questions (10 point)
2. Describe and discuss the cardinal principles used in meat inspection.
3. Briefly discuss head inspection procedure
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Information Sheet 4- Taking and preparing samples of organs, tissues and
fluid
Introduction
Recognizing and recording abnormalities are enhanced by developing a consistent routine in the
dissection and collection of tissues. There is often a tendency to move quickly to the suspected
lesion or body system, which risks missing important information. A good necropsy involves
paying attention to ALL the clues that can be provided, so the routine has to be followed, with
attention to detail at every step.
1. Obtain the history: A complete individual animal and herd history should be obtained.
This history should include: age, breed, and sex of affected animals, husbandry conditions
(including housing, feed), clinical signs, and any treatments administered and whether the
animal died or was euthanized.
2. External examination: Many people believe a necropsy begins when you start using the
knife. But if you neglect to look at many external aspects of the carcass, some key findings
can be missed.
Figure 16. Examine the site where the animal was found for clues: predators (dogs), lightning,
poisons and poisonous plants, signs of trauma.
Evaluate the general body nutritional and hydration status.
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Look at the external orifices for any discharges and for color of mucous
membranes. Look at teeth for age and abnormal wear.
Are there any skin lesions or external parasites?
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esophagus (“pluck”), rumen/abomasum/intestines, liver, urogenital tract, reproductive tract,
brain.
In examining and sampling, we usually go from the “cleanest” to the “dirtiest”. Usually this
order is: lymphoid tissue, brain, lungs, heart, kidneys, reproductive tract, liver, intestinal tract.
Note any abnormalities for each (color, size, shape, consistency, exudates). Be sure to examine
both capsular and cut surface. Make several cuts in each organ. Collect specimens for further
diagnostic work.
Sampling of Tissues
Tissues should be collected in duplicate, with half going into 10% formalin for histopathology
and half as unfixed tissues that will be used for bacterial culture, virus isolation, fluorescent
antibody testing, toxicology, etc. The following tissues should be collected in all cases: lung,
liver, spleen, heart, brain, kidney, intestines. Additionally, any other tissue that might have
lesions (skin, adrenal, ovary, etc.) should also be collected. Crushing should be avoided when
taking samples since this may cause histological artifacts. This can be avoided by using a sharp
blade and a hard surface.
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Figure 20. Tissue sample collected from different organs
When examining any organ with a mucosal surface (trachea, esophagus, intestine, etc.) care
should be taken not to damage or destroy the mucosal surface by rubbing the surface with
fingers or instruments.
Samples of tissue from a variety of organs can be taken at post-mortem Animal health personnel
should be trained in the correct procedures for post-mortem examination of the species of
animals with which they work. The equipment required will depend on the size and species of
animal, but a knife, saw and cleaver will be required, and also scalpel, forceps and scissors,
including scissors with a rounded tip on one blade, for opening intestines. A plentiful supply of
containers and tubes of transport media appropriate to the nature of the sample required should
be available, along with labels and report forms. Containers should be fully labelled with the
date, tissue and animal identification. Special media may be required for transport of samples
from the field. The operator should wear protective clothing: overalls, washable apron, rubber
gloves and rubber boots. Additionally, if potential zoonotic diseases are being investigated, the
post-mortem examination should be conducted in a biological safety cabinet; if this is not
possible, an efficient face mask and eye protection should be worn. If rabies or transmissible
spongiform encephalopathies (TSEs) are suspected, it is usual to detach the animal’s head.
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Tissues may be collected for
Microbiological culture,
Parasitology,
Biochemistry,
Histopathology and/or
Immunohistochemistry, and
For detection of proteins or genome nucleic acids.
In addition buccal, oropharyngeal or rectal (cloacal) swabs may be collected. The person
conducting the post-mortem examination should have sufficient knowledge of anatomy and
pathology to select the most promising organs and lesions for sampli ng. Each piece of tissue
should be placed in a fully labelled separate plastic bag or sterile screw-capped jar. Swabs should
always be submitted in appropriate transport media. Sterile instruments should be used for
collecting specimens for microbiological culture and care should be taken not to contaminate
tissues with intestinal contents. Disinfectants should not be used on or near tissues to be
sampled for bacterial culture or virus isolation. The tissues may be sent to the laboratory dry or
in bacterial or virus transport medium, depending on the type of specimen and the examinations
required; swabs should be sent in transport medium. After collection, the samples for
microbiological examination should be refrigerated until shipped. If shipment cannot be
made within 48 hours, the samples should be frozen; however, prolonged storage at
–20°C may be detrimental to virus isolation. For histopathology, blocks of tissue not more
than 0.5 cm thick and 1–2 cm long are cut and placed in neutral buffered 4–10% formalin,
which should be at least ten times the volume of the tissue sample.
For certain suspected diseases, larger portions of brain are required; the brain is sectioned using a
sagittal cut, half is submitted fresh, on ice, and the other half is submitted in 10% buffered
formalin. For scrapie, bovine spongiform encephalopathy and other TSEs. Store and pack
formalin-fixed tissues separately from fresh tissues, blood and smears. Care should be taken to
insure that formalin-fixed tissues are not frozen. Once fixed, tissues can be removed from
formalin and, as long as they are kept
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moist and protected (e.g. by wrapping in formalin-soaked paper towels, then sealed in screw-
capped jars), they can be forwarded to the laboratory without formalin.
Lymphoid system: The spleen should be relatively flat, not turgid, and will not fracture when
folded. On cut surface, it should be uniform
Lymph nodes should not bulge on cut surface and should have a uniform homogeneous,
white-tan appearance. An exception is the mesenteric lymph nodes which usually have dark
(brown-black) pigment at their centers. This is normal in ruminants.
Brain: Often the brain is sliced down the middle to create symmetrical halves for frozen and
formalin specimens.
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Figure 23. Heart tissue sampling
It is important to examine all chambers of the heart. This can be confusing. The best way is to
open the left ventricle first. Use a knife to make a vertical cut from apex to base over the left
ventricle, then use scissors or a knife to extend the cut up through the left atrium and into the
aorta. This will allow to see both left chambers and both left valves on the left side (left a-v
valve and aortic valve).
Respiratory system: Begin by cutting down the trachea and into the bronchi, making note
of any mucus, froth, or petechial.
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Figure 25: Kidney inspection and sampling
Reproductive tract: At left is the female reproductive tract. Slice through the ovaries. Also
cut into the uterine horns, uterine body, and cervix, observing mucosal surfaces. Cut into the
testes to look at the parenchyma.
Liver: The liver’s surface should be examined for any abnormalities. It should be palpated for
any nodules, friable areas, or other abnormal changes. Several slices are made into the liver in
order to examine the deeper structure of the liver.
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Intestinal tract: Spread out the intestines and determine the various parts. Begin with the
duodenum and follow down the jejunum, to the ileum, which empties into the cecum at the ileo-
cecocolic junction. The large intestine slowly narrows to become the spiral colon, which then
progresses on down to the terminal colon.
Open segments of the intestinal tract, beginning with the duodenum and working caudally. Be
sure to examine the mucosal surface carefully and open numerous segments. If the history
indicates an intestinal problem, you may need to open the entire intestinal tract. Because of the
way you removed the tract, the abomasum is still attached to the forestomaches. Open the
abomasum first. Remember this is the only glandular part of this mass. Look at the contents of
the abomasum and examine the mucosal surface. You may need to run some water lightly over
the mucosa to get a good view. Then open the reticulum and the omasum. Last will be the rumen
itself. Remove some of the contents and look at the pillars and the papillae. Also note the
consistency of the contents.
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Self-Check 4 – Written Test
Name ID Date
Directions: Answer all the questions listed below. Examples may be necessary to
aid some explanations/answers.
Test I. Write true if the statement is correct/False if it is incorrect for the following
questions (4 point each).
1. Recognizing and recording abnormalities are enhanced by developing a consistent routine
in the dissection and collection of tissues.
2. In examining and sampling, we usually go from the “cleanest” to the “dirtiest”.
3. For histopathology, blocks of tissue not more than 0.6 cm thick and 3cm long are cut and
sampled.
Test II. Choose the best answer for the following questions (4 point).
1. Tissues collected in duplicate, with half going into 10% formalin for histopathology and
half as unfixed tissues that will be used for:
A. Bacterial culture
B. Virus isolation
C. Fluorescent antibody testing,
D. Toxicology E. All of the above
2. A good necropsy should pay attention to all of the consistent routine entails ecept:
A. Histroy of the pateint
B. Eternal examination
C. Open the body
D. Removing organs
E. Examine and sample the organ
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Information Sheet 5- Conducting inspection works on meat
Inspection of heads
A head: which has been skinned and washed clean is ready for inspection after the base
of the tongue has been detached so as to give access to the masticatory muscles and
lymph nodes. Where head loops are used the lymph nodes may be incised and examined
before the tongue is dropped.
The head including the oral and nasal cavities should be viewed. The sub- maxillary
(lymphonodi mandibulares), parotid (lymphonodi parotid) and retropharyngeal lymph
nodes (lymphonodi retropharingei) should then be examined by viewing and multiple
incisions. The tonsils should be removed after inspection. The muscles of mastication
should be viewed and incised. One or more linear incisions should be made parallel to
the lower jaw into the external and internal muscles of mastication. The tongue should be
viewed and palpated and, if necessary, an incision should be made into the root of the
tongue without mutilation of the blade. The lips and gums should be viewed.
Inspection of viscera
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in length. Where necessary for reaching a 'diagnosis one or more incisions should be
made deep enough to open up bile ducts and liver substance. The gall bladder should be
viewed and, if necessary, palpated.
Lungs: viewing and palpation of the entire organ, viewing and incision of bronchial and
mediastinal lymph nodes (lymphonodi tracheobronchial mediastinal), provided they are
not obviously diseased. If necessary an incision should open up the larynx, trachea and
bronchi. A transverse incision across the lower part of the diaphragmatic lobe should
open up the bronchi,
Heart: viewing of the heart after the pericardium has been opened. One or more incisions
should be made from base to apex or alternatively the heart may be everted and shallow
incisions made so that the cardiac valves and muscle tissue can be inspected.
Uterus: viewing, palpation and, if necessary, incision.*)
Udder: viewing of the udder, palpation and, if necessary, incision. When the udder has
been or is in. lactating state, incision) of the supra-mammary lymph nodes (lymphonodi
inguinal superficial) should be a routine procedure. If the udder is destined for human
consumption, incision of the organ itself should be a routine procedure.
Kidneys: enucleation,' viewing and, if necessary, palpation and incision.
Testicles: (where they are saved for human consumption): viewing and palpation. If
incisions are made, every precaution should be taken to prevent contamination of the
premises, equipment and personnel.
Inspection of carcasses
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Cleanliness;
Odours.
The skeletal muscles, including the. attached fat and connective tissues, the bones,
especially those which have been cut and exposed during the splitting of the carcass, the
joints, the tendon sheaths, the diaphragm and the pleura and the peritoneum, should be
viewed and, if necessary, palpated and incised. If necessary the triceps brachial
muscle may be incised.
The following lymph nodes should be viewed, palpated and, if necessary, incised:
Superficial inguinal (supra-mammary) (lymphonodi inguinal superficial);
External and internal iliac (lymphonodi iliaci);
Pre-pectoral (lymphonodi cervical profundi caudales);
Renal (lymphonodi renales).
In all animals reacting to the tuberculin test and in those carcasses and viscera in which
tubercular lesions are found, the main carcass lymph nodes should be examined. A
similar procedure should be carried out in all cases in which a systemic or generalized
disease is suspected to exist.
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In general inspection of meat is a value chain approach
Handling of slaughter
of animals Inspection
Transport and hygiene Inspection of
of bleeding, dressing,
from farm to abattoir personnel of
evisceration, splitting,
slaughterhouses
washing
Lairaging and
Handling Post-mortem Management of waste
inspection of carcass from slaughterhouses
hygiene
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Self-Check 5 – Written Test
Name ID Date
Directions: Answer all the questions listed below. Examples may be necessary to
aid some explanations/answers.
Test I. Write true if the statement is correct/False if it is incorrect for the following
questions (4 point each).
1. In all animals, in which their carcasses and viscera had tubercular lesions, the main carcass
lymph nodes should be examined.
2. During eamining carcass, if necessary, triceps brachial muscle, should be incised.
Test II. Choose the best answer for the following questions (4 point).
1. The post mortem carcass examination should be to ascertain:
A. Condition;
B. Efficiency of bleeding;
C. Colour;
D. Condition of serous membranes
E. All of the above
Test III. Short Answer Questions (10 point)
1. Describe and discuss post mortem inspection of bovine animals.
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Information Sheet 6- Disposing unfit offal and waste products
Introduction
All dead animal cadavers and condemned meat should be disposed carefully under the custody of
the meat inspector until properly disposed. However condemned organs and carcasses can be
directed to the by-product processing plant in bigger abattoirs. Rendering of raw animal waste
involves a series of drying and separating processes by which the material is sterilized and the
fats and proteins are extracted to produce tallow and meat-and-bone meal. At the start of the
process, the waste material has a water content of up to 70%; its removal involves relatively
high-energy costs. The water effluent produced also needs to be treated to avoid pollution. The
organic nature of the material creates further problems of odour pollution, requiring additional
pollution abatement technology.
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Manure, paunch and viscera contents
These must be disposed of in a manner which will not create a sanitary problem on the premises
of the registered slaughter establishment. Storage of such wastes in the vicinity of the registered
establishment is unacceptable.
Manure, Compost and Biogas
Digestive and excretory wastes of ruminants, collectively referred to as manure are a mixture of
dung and urine and occur in two forms:
As sweepings from liairages which are built into heaps outside the slaughter building and
occasionally collected in small quantities by small-scale farmers to enrich soil fertility.
And secondly as kraal manure which may remain permanent on the holding ground. Kraal
manure is less preferred because it is often sodden with water (from rains) or mixed with
earth from treading by the animals as well as straw from bedding, thus creating problems
in collection and spreading on farms.
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Self-Check 6 – Written Test
Name ID Date
Directions: Answer all the questions listed below. Examples may be necessary to
aid some explanations/answers.
Test I. Write true if the statement is correct/False if it is incorrect for the following
questions (2 point each).
1. All dead animal cadavers and condemned meat should be disposed carefully under the
custody of the meat inspector until properly disposed
Test II. Choose the best answer for the following questions (3 point).
1. is a process whereby organic material such as animal waste is broken down or
degraded by micro-organisms operating in an oxygen-free environment.
A. Incineration
B. Landfill
C. Anaerobic digestion
D. All of the above
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Operation Sheet: 1. Dissection/Splitting carcass
Materials: (Hook, record book, pen, Carcass splitting machine, carcass, carcass storage room)
Procedure:
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Operation Sheet: 2. Procedure of Post mortem Inspection
Materials: (Record book, pen, carcass, offal, knife, glove, water, table inspection room)
Procedure:
Step 1. Routine post-mortem inspection should include viewing, palpation and, where
necessary, incision. This inspection should be carried out in a hygienic and systematic
manner.
Step 2. Where a lymph node, organ or any carcass tissue is being incised by the inspector,
the cut surface should be cleanly sliced in order to present a picture which is not distorted.
Step 3. The head, organs, viscera and any other part of a carcass required for
post-mortem inspection should be identifiable with the carcass from which they were
removed until inspection has been completed.
Step 4. Except with the permission of the inspector no person should, prior to the
inspection of any carcass being completed:
Remove any serous membrane or any other part from the carcass; or
Remove, modify, or obliterate any evidence of disease in the carcass or organ by
washing, scraping, trimming
Remove any mark or identification from the carcass, head or viscera until the
inspector has completed his inspection and given his decision.
Step 5. No person should remove from the inspection area of an abattoir any part of
any carcass, organ, or any viscera, until the inspector has completed his examination and
a decision has been given.
Step 6. Viscera should be examined: As they are removed from the carcass
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Operation Sheet: 3. Procedure of post mortem sample
taking
Materials: (glove, sampling bottle, tray, knife, gown, scalpel, scalpel blade, animal)
Procedure:
Step 5: Examine and take the appropriate sample from the organ
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LAP TEST
Name ID Date
Instructions: Given necessary templates, tools and materials you are required to
perform the following tasks within 2 hour. The project is expected from each
student to do it.
During your work: You can ask all the necessary tools and equipment
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LG 63
LO 4. Complete the Post-mortem Examination
Instruction sheet
This learning guide is developed to provide you the necessary information regarding the
following content coverage and topics:
Passing judgment for consumption on carcass based on ante mortem and post mortem
examination results
Labelling and taking whole or part of carcass with suspicious signs
Labelling and packaging samples sent for analysis
Collecting information of examined animal with the records of observations
during the post-mortem
Cleaning, disinfecting and storing equipment used in post mortem area
hygienically
Completing personal biosecurity clean-up
This guide will also assist you to attain the learning outcomes stated in the cover page.
Specifically, upon completion of this learning guide, you will be able to:
Pass judgment for consumption on carcass based on ante mortem and post mortem
examination results
Label and take whole or part of carcass with suspicious signs
Label and package samples sent for analysis
Collect information of examined animal with the records of observations during the
post-mortem
Clean, disinfect and store equipment used in post mortem area hygienically
Complete personal biosecurity clean-up
Learning Instructions:
1. Read the specific objectives of this Learning Guide.
2. Follow the instructions described below.
3. Read the information written in the “Information Sheets”. Try to understand what are
being discussed. Ask your trainer for assistance if you have hard time understanding
them.
4. Accomplish the “Self-checks” which are placed following all information sheets.
5. Ask from your trainer the key to correction (key answers) or you can request your trainer
to correct your work. (You are to get the key answer only after you finished answering
the Self-checks).
6. If your performance is satisfactory proceed to the next learning guide,
7. If your performance is unsatisfactory, see your trainer for further instructions.
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Information Sheet 1- Passing judgment for consumption on carcass based on
ante mortem and post mortem examination results
Introduction
Classifying the lesions into one of two major categories - acute or chronic.
Establishing whether the condition is localized or generalized, and the extent of
systemic changes in other organs or tissues.
Determining the significance of primary and systemic pathological lesions and their
relevance to major organs and systems, particularly the liver, kidneys, heart, spleen
and lymphatic system.
Coordinating all the components of ante mortem and postmortem findings to make a
final diagnosis.
Submitting the samples to the laboratory for diagnostic support, if abattoir has holding
and refrigeration facilities for carcasses under detention.
Carcass judgment
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If the food animal is infected with infectious, contagious disease or zoonotic disease.
If residues beyond the normal limit is detected
If there is severe organoleptic deviation from normal meat
The meat has been conditionally approved for human consumption, but this has not
been treated by heat or cold.
3) Partially condemned for human consumption: This judgment is passed, if
abnormalities or defects are encountered in certain parts of the organ or carcass. .
4) Conditionally approved for human consumption: This judgment is passed, if the
carcass or organ poses hazards to the public or animal health. But the hazard can be eliminated
through proper treatment
5) Inferior meat: if the meat is below accepted standard, but does not pose public hazard
when consumed.
6) Approved for human consumption with distribution restricted to limed areas:
The judgment is passed, if
The animal originated from an area where there is an outbreak of disease and
slaughtering has been recommended to contain the disease in that area or
The animal comes from quarantine stations.
7) Detained: carcass may be detained for further inspection under
Slight discoloration e.g. as in the case of jaundice
If chemical, toxicological or microbiological examination is required.
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Self-Check 1 – Written Test
Name ID Date
Directions: Answer all the questions listed below. Examples may be necessary to
aid some explanations/answers.
Test I. Write true if the statement is correct/False if it is incorrect for the following
questions (4 point each).
1. Inspector should be coordinating all the components of ante mortem and
postmortem findings to make a final diagnosis.
2. Conditionally approved for human consumption is passed, if the carcass or organ poses
hazards to the public or animal health and but the hazard can be eliminated through
proper treatment.
Test II. Choose the best answer for the following questions (4 point).
1. If the slaughter operation has been performed in hygienic manner and no disease or
abnormalities as well as drug residues does not detected at post mortem inspection, what was
the final judgement should be passed by the inspector regarding the carcass and offals?
A. Totally condemned
B. Approved for human consumption
C. Conditionally approved for human consumption
D. Approved for human consumption with distribution restricted to limed areas
Test III. Short Answer Questions (8 point)
1. What was the reason behined on the dettection of meat for the reasonable period of time?
Explain it.
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Information Sheet 2- Labelling and taking whole or part of carcass with
suspicious signs
Introduction
Samples shall be taken from processing, storage areas, equipment and meat used in food
production or at point of production or sale, when such sampling is necessary for ensuring that
the criteria are met.
It is essential that individual samples be clearly identified using appropriate methods. Marking
instruments should be able to withstand the condition of use, i.e. being wet or frozen (use
indelible marking pen). Pencil has a tendency to rub off containers and labels attached to plastic
will fall off when stored at –70°C. Information and case history should always accompany the
samples to the laboratory, and should be placed in a plastic envelope on the outside of the
shipping container. As outlined in the following section on transport of samples, this information
must also be inside the shipping container. The following are suggested items that should be
addressed. It would be advisable to contact the receiving laboratory to determine if it has a
submission form that it would like to have submitted with the samples or if it needs other
information.
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The length of time sick animals have been on the farm; if they are recent
arrivals, from where did they originate.
The date of the first cases and of subsequent cases or losses, with any
appropriate previous submission reference numbers.
A description of the spread of infection in the herd or flock.
Collection and shipment of diagnostic specimens
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Self-Check 2 – Written Test
Name ID Date
Directions: Answer all the questions listed below. Examples may be necessary to
aid some explanations/answers.
Test I. Write true if the statement is correct/False if it is incorrect for the following
questions (4 point each).
1. Samples shall be taken from processing, storage areas, equipment and meat used in food
production.
2. Information and case history should always accompany the samples to the
laboratory, and should be placed in a plastic envelope on the outside of the
shipping container.
Test III. Short Answer Questions (6 point)
1. What are the information should be incorporated with the sample while sending to the
referal laboratories?
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Information Sheet 3- Labelling and packaging samples sent for
analysis
Introduction
Enclose specimens in a secure container and label the container with a waterproof pen. Place this
container in a waterproof bag with tissue, towels or other blotting material to absorb any leakage.
Put all specimen containers in an insulated box packed with ice or frozen refrigerant packs and
deliver them to the laboratory as soon as possible. If sending specimens by post or courier ensure
that they are delivered during business hours on a weekday.
Packaging: Packaging of fresh, chilled and frozen products shall be done in food grade
packaging materials that protect the meat product from any physical, microbiological, chemical
or any other type of contamination during storage, distribution and handling.
Labelling: Labels and marking on the packaging are an essential source of information to
communicate to everyone involved in the transportation process the contents of the package, the
nature of the hazard and the applied packaging standards. Most “certified” shipping containers
already include the appropriate labels and markings as part of the package. All markings must be
placed on the packaging so that they are not covered or obscured by any part of, or attachment to
the packaging, or any other labels or markings. All markings must be:
Durable and printed or otherwise marked on, or affixed to, the external surface of the
packaging
Readily visible and legible
Able to withstand open weather exposure without substantial reduction in effectiveness;
Displayed on a background of contrasting colors
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Address the sample clearly with labelling including the following information:
The name and telephone number of the receiving laboratory.
Name/initials of researcher
Date sample prepared
Sample identification such as:
Identification number
Sample name
Species name
Location where sample was collected (coordinates, address, etc.)
Variables that may affect sample, such as: air temperature, altitude, water depth,
humidity, etc. during collection
Time of collection
Type of sample the name of the organisation or individual conducting the
research, or making the collection;
The name of the abattoir of origin;
The kind and amount of sample removed;
The purpose of collection; and
How the material must be disposed of after the intended use, where applicable.
The approval of the owner of the plant is required for the arrangements for the
collection of specimens.
Properly Label Samples
Reduces risk of contamination
Reduces injury to self and others
Maintains clean and organized working environment
Avoids loss or damage to valuable samples
Maintains integrity of research being conducted
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Raw meat or poultry: Use one of the following methods:
Using a sterile utensil or sterile glove, place carcass or large piece of meat in a large
sterile plastic bag.
Add 100–300 ml enrichment broth.
Remove sample and seal the bag.
Wipe a sterile sponge over a large section of the carcass or piece of meat.
Place swab in a jar containing enrichment broth.
Moisten a swab in buffered distilled water or 0.1% peptone water.
Wipe the swab over a large section of the carcass or piece of meat.
Place swab in enrichment broth.
Using a sterile glove wipe the carcass or the piece of meat with sterile gauze pads
and place the pads in a jar containing enrichment broth.
Aseptically cut a piece of meat or skin from different parts of the carcass or large piece
of meat, or remove part of the carcass. Place at least 200 g of sample in a sterile plastic
bag or glass jar; refrigerate.
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Self-Check 3 – Written Test
Name ID Date
Directions: Answer all the questions listed below. Examples may be necessary to
aid some explanations/answers.
Test I. Write true if the statement is correct/False if it is incorrect for the following
questions (4 point each).
1. Enclose specimens in a secure container and label the container with a waterproof pen is
essential.
2. Packaging should be durable and printed or otherwise marked on, to, the external surface
of the packaging.
3. Labels and marking on the packaging are an essential source of information to
communicate to everyone.
Test II. Choose the best answer for the following questions (4 point).
1. Which one of the following is not the advantage of proper labbelling?
A. Reduces risk of contamination
B. Reduces injury to self and others
C. Maintains clean and organized working environment
D. Avoids loss or damage to valuable samples
E. None of the above
Test III. Short Answer Questions (10 point)
1. Describe all essential information accompany packaging marking.
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Information Sheet 4- Collecting information of examined animal with the records
of observations during the post-mortem
Introduction
Meat traceability is ability to follow the movement of a meat product through specified stage(s)
of animal production, slaughter, processing and distribution of meat products. An extensive form
of traceability is the ability to follow meat products forward from their source animal (at birth)
through growth, slaughter, processing and distribution to the point of sale or consumption, (or
backward from the consumer to the source animal). Animal identification is one component of
meat traceability. Inspection personnel must also record information about the number of animals
or birds slaughtered, the number and types of products condemned, and other details.
The food/meat inspector is to complete the appropriate sections of the lot tally Sheet including
the:
Abattoir name and address
Telephone Number
Inspection date
Shift of inspection
Establishment number
Specific production (lot number)
Class of slaughtered animals
Condemned part/whole carcass
Condemnation reason
Number of condemnations for each category
Type of organ condemned and reason of condemnation
Name, Date and signature of inspector
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The food inspector gives the Lot Score Sheet to the “inspector’s helper” at the beginning of each
shift. The “inspector’s helper” records the condemnations throughout the shift. Record the detail
information and parameter on post-mortem inspection card
Heart
- Judgement -
Lung -
-
Judgement -
Liver and Kidney
- -
Judgement
Carcass
-
Judgement -
GIT organs inspection result
Judgement - -
Name of Inspector -
Signature Date -
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Self-Check 4 – Written Test
Name ID Date
Directions: Answer all the questions listed below. Examples may be necessary to
aid some explanations/answers.
Test I. Write true if the statement is correct/False if it is incorrect for the following
questions (5 point each).
1. Meat traceability is ability to follow the movement of a meat product through
specified stage(s) of animal production.
2. Animal identification is not one component of meat traceability
Test II. Choose the best answer for the following questions (5 point).
1. Which information should be recorded on the record sheet during post mortem
examination
A. Judgement of inspection
B. Class of slaughtered animals
C. Condemnetion reason
D. Type of organ condemned and reason of condemnation
E. All of the above
Test III. Short Answer Questions (10 point)
1. Prepare post mortem record format and conduct inspection of one animal at your local
municipal abattoir.
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Information Sheet 5- Cleaning, disinfecting and storing equipment used in
post mortem
Slaughterhouse cleaning
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wetting, reduces surface tension and makes penetration possible. Agents are: hot water, soaps
and synthetic detergents, which are rather fragile suds formers.
Suspension. An agent is required to hold the loosened soil in the washing solution so it
can be flushed away and not re-deposited. For fat particles, an emulsifying action is required to
saponify the fat and carry it away. Soap, highly alkaline salts, and non-ionic synthetics may be
used. For protein particles, colloidal solutions must be formed by peptizing (known also as
sequestering or deflocculating). This action prevents curd formation in hard water.
Rinsing. The agent used must remove and flush away soils and cleaners so they are not
redeposited on the surfaces being washed. Clean, clear hot water is usually effective alone. The
walls, equipment and floor should be rinsed well with cold or more potable water to remove the
detergent before application of a suitable disinfectant.
Disinfection. This is the process of destroying pathogens on slaughterhouse
equipments, containers and working surfaces to make them safe for intended use. Disinfection is
achieved by use of disinfectants. Disinfectant is chemical agent that destroys most pathogens but
may not kill bacterial spores. Disinfectants are chemicals used to destroy microorganism of
equipment used. Most disinfectants do not kill all microorganisms that are present on equipment
and working surfaces, but will reduce their numbers to a level that is that is not harmful to human
health. Sodium hypochlorite is the commonly used disinfectant in food industries. Some bacteria
may develop increased resistance after prolonged contact with some disinfectants. As a result
disinfectants should be changed after every 3-4 months.
Antiseptics. These are chemicals used to kill microorganisms on the skin as in hands
of slaughterhouse workers.
Sterilization. This is the process of removing or destroying all forms of microbial life including
bacterial spores using physical or chemical means. Sterilization is accomplished commonly by
steam under pressure, by dry heat and by chemical sterilants. The choice of the method for
sterilization depends on a number of factors including:
The type of material that the object to be sterilized is made of,
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The number and type of microorganisms involved, and
A availability of sterilization methods.
In many food industries, sterilization of equipments, meat containers and working surfaces is
achieved by steam under pressure, or use of hot water at 90oC. All surfaces and equipment
should be rinsed off with potable water before starting work where chemicals had been applied
and let overnight.
Slaughterhouse cleaning involves dry and wet cleaning operations.
All abattoir equipment should be cleaned properly and stored to their proper soring area. Water
and detergents used to wash and clean the materials and equipment in the abattoir should be
available all the time. Ample supply of good, hygienic, soft and hot water at a temperature of not
less than 82° C and adequate number of hose points are essential. The usual method of applying
hot water in meat plant is through high- pressure jet cleaners with 14kg f/cm2. The manual
operation of spray guns in which the pressure is in the 35-49 kg f/cm2 range, the volume of water
being low, averaging about 9 liters/minute.
Application of detergent followed by sanitizer or a combination of both is necessary for an actual
meat premises under adequate pressure and temperature (not less than 14 kg f/cm2 and 82°C).
Dry cleaning should commence immediately after operations have ceased and should embrace
the whole premises, where disinfectant should be used.
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This good system will ensure the final daily operation after the completion of slaughtering
rendered more effectively. Cleansing operations must be done frequently to prohibit any built-
up of bacteria on trolleys, hooks, gambrels, etc., which come in contact with the meat. Instead of
using highly sophisticated cleansing installations, manual cleansing has been found to be more
effective in some parts of the slaughterhouse.
Compound cleaning
Cutting of grass, clearing of bushes and filling of potholes
Collection of papers, old ropes, cans, horn and tail switches and burning them
Regular collection of manure from shed and subsequent cleaning
Proper disposal of condemned materials in lockable disposal pits
Proper washing and disinfection of the lairage and races,
Pave the external surface of the slaughterhouse, including drive way and vehicle
washing stands
Top part of the condemnation pit and blood tank, grease trap and drains
Meat containers/carriers
Control of birds, insects, rodents cats and dogs
Proper liquid waste disposal
Lairages, race, drains, loading bay, offices and wash rooms, top part of the grease trap,
condemnation pit, blood tank, and manure shed; should be washed on daily basis and
disinfected.
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Self-Check 5 – Written Test
Name ID Date
Directions: Answer all the questions listed below. Examples may be necessary to
aid some explanations/answers.
Test I. Write true if the statement is correct/False if it is incorrect for the following
questions (6 point each).
1. Disinfectant is chemical agent that destroys most pathogens but may not kill
bacterial spores
2. Antiseptics.are chemicals used to kill microorganisms on the skin as in hands of
slaughterhouse workers.
Test II. Choose the best answer for the following questions (5 point each).
1. Dry-cleaning involves:-
A. Collection of dropped waste scraps of meat, fat and pieces of bones
B. Removal of thick deposit on equipment, floor and walls.
C. Use of squeezers to remove blood from the floor
D. All of the above
2. is the process of removing or destroying all forms of microbial life including
bacterial spores using physical or chemical means.
A. Disinfection
B. Sterilization
C. Sanitation
D. Hygiene
Test III. Short Answer Questions (10 point)
1. What is the advantage of hygienic production of meat?
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Information Sheet 6- Completing personal biosecurity clean-up
Introduction
Slaughterhouse staff refers to anybody involved in all stages of slaughter, dressing, cleaning,
inspection, loading and transportation of meat, washing and cleaning of equipment and all
slaughterhouse premises. The attitude of the workers toward cleanliness is important as anything
in the production of clean, wholesome, unspoiled products. Employee health and hygiene (clean
hands, clothing and good hygienic practices) directly or indirectly, plays an important role in
food safety and sanitation.
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Health checks: The hygiene manager must ensure that all personnel are examined
daily, before starting work, for adverse health conditions. People who are suffering from a
contagious disease or carriers of an infectious condition, or who have even been in contact with a
source of contagion, may not work in any part of the abattoir where edible products are handled.
This includes the slaughtering area, rough offal processing areas, storage facilities, cold storage,
de-boning areas, offloading areas
Injuries: Workers who have injuries on the hands and lower portions of the arms such as cuts,
abrasions, suppurating abscesses, sores, burns may not work with edible products unless such
conditions treated immediately and secured with waterproof dressing or plastic glove or the
injury is healed. Personnel must immediately report any injury to the inspectors.
Showering and washing of hands: People normally carry some bacteria on or in their
bodies that can cause foodborne diseases. These are called "normal flora" and most people do not
know they are there. For example, on the average, almost 2/3 of the populations are carriers of
the bacteria that causes Clostridium perfringens food poisoning and one out of every three
persons has Staphylococcus aureus in their nasal passages as normal flora. These important
disease-causing microorganisms that are found on the body is sufficient to contaminate the
hands, during a simple act of touching the nose or blowing the nose. Because hands are so
important in the transmission of disease organisms, they must be properly washed and washed
often.
Effective hand washing include, turn on warm water approximately 43°C to a simple tap which is
foot- or knee-operated, apply liquid disinfectant soap and rub vigorously for at least 20 seconds,
rinse with warm water, dry hands with paper towel, turn off water foot- or knee-operated or if
hand operated use with paper towel.
Hygiene of protective clothing: All meat handlers and persons in a room handling meat
should wear CLEAN protective clothing’s (overcoat, cap, plastic apron, and gumboots). They
should be of light color, preferably white and be clean and tidy. This does not necessarily apply
to workers in liairages/receiving areas and dirty areas.
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Self-Check 6 – Written Test
Name ID Date
Directions: Answer all the questions listed below. Examples may be necessary to
aid some explanations/answers.
Test I. Write true if the statement is correct/False if it is incorrect for the following
questions (4 point each).
1. Biosecurity refers to prevention from transmission of infectious diseases, parasites and
pests from one animal to another.
2. The hygiene manager must ensure that all personnel are examined daily, before starting
work, for adverse health conditions.
3. All meat handlers and persons in a room handling meat should wear clean protective
clothing’s.
Test II. Choose the best answer for the following questions (4 point).
1. Which one of the following is the component of biosecurity slaughtering house?
A. Slaughtering house staff hygiene
B. Coumpound hygiene
C. Equipment and Personal protective equipment hygiene
D. Showering, washing of area hygiene
E. All of the above
Test III. Short Answer Questions (4 point)
1. Describe the importance of biosecurity maintainance.
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LG 64
LO 5. Develop Knowledge of Public Health
Instruction sheet
This learning guide is developed to provide you the necessary information regarding the
following content coverage and topics:
Identifying pathological lesions and zoonotic diseases based on ante mortem and post
mortem inspection
Explaining effect of conducting slaughter in unhygienic area
Assuring meat quality and hygiene maintenance
Describing risk of consuming raw meat and meat products
Undertaking disposal of part of carcass or organs found unfit
This guide will also assist you to attain the learning outcomes stated in the cover page.
Specifically, upon completion of this learning guide, you will be able to:
Identify pathological lesions and zoonotic diseases based on ante mortem and post
mortem inspection
Explain effect of conducting slaughter in unhygienic area
Assure meat quality and hygiene maintenance
Describe risk of consuming raw meat and meat products
Undertake disposal of part of carcass or organs found unfit
Learning Instructions:
1. Read the specific objectives of this Learning Guide.
2. Follow the instructions described below.
3. Read the information written in the “Information Sheets”. Try to understand what are
being discussed. Ask your trainer for assistance if you have hard time understanding
them.
4. Accomplish the “Self-checks” which are placed following all information sheets.
5. Ask from your trainer the key to correction (key answers) or you can request your trainer
to correct your work. (You are to get the key answer only after you finished answering
the Self-checks).
6. If your performance is satisfactory proceed to the next learning guide,
7. If your performance is unsatisfactory, see your trainer for further instructions.
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Information Sheet 1- Identifying pathological lesions and zoonotic diseases
5.1 Introduction
Poor condition /emaciation
Poor body condition/Emaciation is a common condition of food animals and is
characterized by a loss of fat and flesh following the loss of appetite, starvation and
cachexia.
Ante mortem findings:
Wrinkled, dry leathery skin
Rough hair coat
Prominent bones and sunken eyes
Postmortem findings:
Serious atrophy of fat in the carcass and organs especially the pericardial and renal fat
The fat is watery. Translucent or jelly-like and hangs from the intervertebral
spaces
Edema and anemia may develop due to starvation and malnutrition due to
parasite infestations.
Judgment:
On postmortem examination it is important to assess and differentiate emaciation
from leanness. In case of doubt, the carcass may be held in the refrigerated room and
the general setting of the carcass should be examined the following day.
If the body cavities are relatively dry, edema of muscle tissue is not present and
fat is of an acceptable consistency i.e. has “set”, the carcass may be passed for
food.
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Imperfect bleeding
Incomplete bleeding can be caused by stress, ineffective stunning techniques (stun time to long
or short, the stun to bleeding time to long) or an ineffective bleeding cut (throat cut or thoracic
“sticking”). All the visible blood vessels may be blood filled causing the carcass to have an
overall darker red colour. This is also true for organs such as the liver which may be dark purple-
red in colour.
Judgement
Meat from such a carcass will have poor lasting qualities and is condemned.
Fever is an abnormal elevated body temperature. It may be classified as septic and aseptic
according to the presence or absence of an infection. In septic fever the infection is caused by
viruses, bacteria, bacterial toxins, protozoa and fungi.
Ante mortem findings:
Chills and sweating
Dehydration
Elevated body temperature
Increased pulse and respiration
Depression and dullness
Anorexia and obstipation
In septic fever the other signs may include
Diarrhea and vomiting
Shock, convulsions and coma
Post mortem findings:
Rigor mortis
Putrefaction
Congestion of subcutaneous blood vessels and carcass
Enlarged lymph nodes
Evidence of cloudy swelling of liver, heart and kidneys
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Judgment: Carcass is condemned if fever syndrome is associated with presence of bacteria
or bacterial toxins in the blood and/or findings of drugs and antimicrobial substances.
Septicaemia
Ante mortem findings:
Depression
Changes in body temperature. The temperature is usually elevated but it can also be
normal and subnormal during the terminal phases.
Difficult and rapid breathing
Shivering and muscle tremors
Congestion or Petechial hemorrhages of conjunctivae, mouth and vulvar mucosa
Post mortem findings:
Enlarged edematous or hemorrhagic lymph nodes
Degenerative changes in parenchymatous organs (liver, heart and kidneys)
Splenomegaly
Inadequately bled-out carcass as a result of high fever
Blood stained serous exudates in abdominal and/or thoracic cavities.
Anaemia resulting from bone marrow depression and icterus may also be
present.
Judgment:
The animals, animal carcasses, offal and other detached portions of animals
affected with septicaemia are condemned.
Oedema or dropsy
Edema is the accumulation of excess fluid in the intercellular (interstitial) tissue compartments,
including body cavities.
Ante mortem findings:
Depressed and drowsy
Swelling of the mandible, dewlap, legs, shoulder, brisket and abdomen
Edematous tissue is cool upon touch and is of a firm, doughy
consistency.
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Postmortem findings:
Wet, sloppy musculature which pits on pressure
Accumulation of clear yellow fluid in the thorax, abdomen and
subcutaneous tissue
Judgment: Animals affected with generalized edema may be condemned on ante
mortem inspection. In less severe non-generalized cases, animals are treated as “suspects”.
The carcass may be totally or partially condemned depending on the extent
and cause of the condition. The presence of localized edema necessitates
removal of the affected area. The carcass is then approved.
Pigmentation
Melanosis
Melanosis is an accumulation of melanin in various organs including the kidneys, heart, lungs
and liver and other locations such as brain membranes, spinal cord, connective tissue, etc.
Melanin is an endogenous brown-black pigment randomly distributed in tissue.
Judgment:
Carcasses showing extensive Melanosis are condemned. If the condition is localized, only the
affected organ or part of the carcass needs to be condemned.
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Figure 33. Melanin deposits in the sheep viscera.
Jaundice/icterus
Icterus is the result of an abnormal accumulation of bile pigment, bilirubin, or haemoglobin in
the blood. Yellow pigmentation is observed in the skin, internal organs, sclera (the white part of
the eye), tendons, cartilage, arteries, joint surfaces etc.
Figure 34.Jaundice of an aged cow caused by liver disease. Note yellow discoloration of
body fat, lungs, heart and kidneys.
Jaundice is divided into three main categories: Prehepatic jaundice (haemolytic icterus),
Hepatic jaundice (toxic icterus), and Posthepatic jaundice (obstructive icterus).
Prehepatic or Hemolytic jaundice: occurs when there is an excessive break down of
red blood cells before their actual life span by different hemoparasites, bacterial toxins,
systemic infections, and in chemical and plant poisoning.
Hepatic or Obstructive jaundice: occurs when there is an obstruction to the passage of
bilirubin from the liver to the intestine. The most common examples are
gallstones or pressure from any kind of swelling and tumor in the surroundings of the bile
ducts.
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Hepato- cellular jaundice: is the result of extensive damage to the liver cells as a result
of severe hepatitis.
Judgment:
The carcass and viscera with hemolytic, toxic icterus and obstructive icterus are
condemned.
Less severe cases are kept in the chiller for 24 hours. Upon re-examination, the carcass may
be approved or condemned depending on the absence or presence of pigment in the
tissue. If the obstructive icterus disappears after 24 hours, the carcass and viscera can be
passed for human food.
Calcification
Calcification is the deposition of calcium salts in dead and degenerating tissue. It may be
regarded as a body reaction to immobilize some foreign agents.
Judgment:
Gangrene: The death of body tissue (necrosis), generally in considerable mass, usually
associated with loss of vascular supply and followed by bacterial invasion and putrefaction. It
occurs most frequently in tissues susceptible to contamination, e.g. skin, lungs, intestine, vagina,
uterus and those in penetrating wounds. Although it usually affects the extremities, gangrene
sometimes may involve the internal organs. Signs are fever, pain, darkening of the skin, and an
unpleasant odour of the affected site.
Two forms are known: dry and wet (gas). Dry gangrene: little to no blood supply to the area,
lesions are dry, light brown in colour and have a leathery appearance. Gas or wet: Anaerobic
spore forming bacteria (Clostridia) form gas. Lesions which are gas filled may also contain blood
tinged serum. Putrefaction of necrotic tissue causes foul smelling colour is purple-green-brown
to black colour.
Causes
Mainly poor blood supply (hypoxia) – Freezing, snares etc.
Foreign body drawn into the lungs
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Torsion of organs
Contaminated wounds
Judgement
Unless the gangrene is much localised and there is no evidence of toxaemia the carcass and
offal is rejected.
Haemorrhage
Hemorrhage is seen at slaughter in various organs, mucous and serous membranes, skin,
subcutaneous tissue and muscles. It may be caused by trauma, acute infectious diseases or
septicemia.
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If local treatment and withholding periods are observed, the carcass and viscera are
approved.
Sexual odour in a carcass can have a limited distribution according to the consumers
taste. Extremely strong sexual odour requires condemnation of the carcass.
A carcass which gives off a pronounced odour of medicinal, chemical or other foreign
substances shall be condemned. If the odour can be removed by trimming or chilling, the
carcass may be passed for human food after the removal of affected parts or dissipation
of the condition.
Abscesses (pyemia)
An abscess is a localized collection of pus separated from the surrounding tissue by a fibrous
capsule.
Bruise
Bruises are frequently found on ante mortem and post-mortem examination in food producing
animals and poultry. In cattle bruises caused by transportation or handling are commonly found
in the hip, chest and shoulder areas; in pigs within the ham and in
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sheep in the hind leg. Bruises and hemorrhage in the hip joint are caused by rough handling of
animals during shackling. Bruises in poultry can be localized or generalized and are frequently
associated with bone fractures or ruptured ligament tendons.
Judgment:
Carcasses affected with local bruising are approved after being trimmed. Carcasses affected with
bruises or injuries associated with inflammatory lesions are also approved if tissue reaction does
not extend beyond the regional lymph nodes. The affected area should be condemned. When
bruises or injuries are associated with systemic change and the wholesomeness of the
musculature is lost, the carcass will be condemned.
Immaturity occurs mainly in calves. The slaughter of calves younger than two weeks of age is
prohibited.
Ante mortem and post mortem findings:
Presence of the umbilical cord
Bluish and not completely retracted gums
Grayish muscles are flabby, tear easily and are not well developed
Dark red kidney and edematous kidney capsule
Judgment: Carcass and offal of immature animals are condemned
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Major parasitic, bacterial, viral zoonotic diseases
Zoonotic diseases: - are diseases that are transmitted from animal to human beings
i) Parasitic zoonotic diseases
Taeniasis is a parasitic (tapeworm) disease of the small intestine of man due to T. saginata (adult
stage). Cysticercosis is the tissue infection of cattle due to larval stage or cystic stage of
Saginata called Cysticercus bovis (beef tapeworm). Taeniasis and Cysticercosis are common
where beef meat is eaten raw or imperfectly cooked.
Taeniasis due to T. solium is an infection of the small intestine of man with the adult stage of the
pork tapeworm. Cysticercosis is the tissue reaction with its larval or cystic stage (Cysticercus
cellulosae / ‘pork measles’), which occurs most commonly in the musculature of the pig.
Predilection sites for C. cellulosae (Cysticercosis) are heart, diaphragm, and internal masseter,
tongue, neck, shoulder, intercostal and abdominal muscles. The liver, lungs, kidneys, eye and
brain are less often affected. The deep muscles of the thigh are often involved so that absence of
infection of the usual sites does not necessarily indicate the absence of cysts in the carcass
musculature. Cattle come into contact with tapeworm eggs while grazing in these pastures and
consume them with the pasture. The eggs develop into larvae, which then penetrate the intestinal
mucosa and are transported through the circulatory system and eventually lodge in their muscles
where they occur as cysts. When man ingests cattle meat having cysts, larvae are liberated from
the cyst which then develop into adult worms.
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Judgment:
In countries where pork measles seldom occur it warrants total condemnation of the
carcass and organs. Example, Ethiopia
In countries where they occur frequently:
Slight infection: conditionally passed. The treatment should be by cold or heat
as described in case of beef measles.
Generalized infection warrants total condemnation.
Prevention and control measures
Proper meat inspection to identify infected meat
Proper disposal of sewage and other wastes to prevent contamination of pastures
Construction and use of toilets/latrines
Regular deworming of humans
Regular deworming of cattle
Adequate cooking/roasting of meat
c) Echinococus granulosus (Hydatid cyst):
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Figure 41. Hydatidosis in liver
The size of hydatid cysts in animals varies from that of a marble to a small football; they contain
a clear watery fluid in which brood capsules may be floating around (so called hydatid sand).
They are surrounded by a reactive connective tissue capsule. Sometimes the cyst degenerate
becomes smaller with the fluid being replaced by caseous material, which may calcify.
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Figure 43. Echinococcosis/hydatid disease in humans
Judgment:
Condemnation and destruction of affected organs
Total condemnation if cysts are found in muscles or bones (very rare case)
Preventive measures
Creation of public awareness through health education,
Meat inspection in farms and abattoirs to identify and remove affected organs so that
they are not sold for human consumption,
Periodic diagnostic testing of dogs.
Control of livestock slaughter by centralization of slaughtering to ensure animals and
carcasses are inspected,
Efficient meat inspection and proper disposal of affected offal (e.g. disposal in
properly constructed ‘condemnation pits’.)
Dog owners to control movement of their dogs.
Regular deworming of dogs with suitable anthelmintic e.g praziquantel
Prevent dogs from gaining access to infected offal in slaughterhouse and
surroundings.
Reduction of dog numbers by elimination of stray and surplus dogs
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d) Trichinellaspirallis (Trichinella cyst)
It is a parasite of great public health importance in which the adult worm is found in the small
intestine of man, pigs, rats, dogs, cat, and other mammals. The larvae enclosed in oval cysts
(lemon-shaped) with their long axis parallel to the muscle fiber. The cysts measure about 0.5 by
0.25mm. Even though the cyst can remain alive for many years it tends to become calcified.
Infection results from the consumption of raw or undercooked flesh of animals containing viable
encysted larvae.
Judgment: as man may acquire the infection by eating infected pork
The judgment is total condemnation.
e) Faschiolosis or liver fluke disease:
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ii) Bacterial Zoonotic Diseases
a) Anthrax
This is an acute bacterial infection caused by Bacillus anthracis that occurs frequently in
herbivorous animals. However, all warm blooded animals are susceptible to varying degrees.
Grazing animals are infected when foraging in areas contaminated with spores of B. anthracis.
The disease is typified by sudden death in domestic and wild animals. Terminally ill animals
bleed from the nose, mouth, and bowel thus contaminating soil or watering place with the
bacteria. The disease has a worldwide distribution. Human infection occurs as a result of contact
with animals that have anthrax through:
1) Butchering and skinning
2) Consumption of contaminated meat
3) Contact with contaminated hides and skins, goats hair, wool or bones.
Ante mortem findings:
The peracute and acute forms in cattle and sheep are without clinical signs. Death may
follow in the acute form after 1 – 2 hours of illness. The acute form lasts about 48 hours.
Postmortem findings:
Dark-tarry blood discharge from body orifices
Absence of rigor mortis
Hemorrhage of the mucous and serous membranes, lymph nodes and subcutaneous
tissue
Enlarged spleen
Severe hemorrhagic enteritis
Degeneration of the liver and kidneys
Bloating and rapid decomposition of carcass
Localized lesions in the intestine of pigs (dysentery)
Diagnosis of anthrax is carried out by direct microscopic examination of tissues and fluids
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Figure 45. Bacillus anthracis.
Judgment: Condemnation of the carcass and its parts by burning or burial. If disposed
by burial, the carcass should be buried at least 6 feet below ground.
Control measures
In man:
Control of infection in animals as described below
Prevention of contact with infected animals discharges and or products
Environmental and personal hygiene where animals products are handled
Prompt treatment of infected persons
Disinfections of fur and wool with hot formaldehyde
Vaccination of high risk groups
Animals
Prompt diagnosis
Vaccination in endemic areas - Animal with Bacillus anthacis
Deep (6 feet) burial or incineration of unopened cadavers. In case of burial, the body of
the animal should be covered with quicklime and then covered with soil. The grass and
soil in the vicinity of the cadaver is buried and area covered with quicklime.
Affected herds should be quarantined for 2 weeks from last case with no animal or
product allowed out of the farm.
In slaughterhouse – all operations must be halted until diagnosis is confirmed. If
positive, all exposed carcasses must be destroyed and premises carefully disinfected
with caustic soda within 8 hours. Animals that are confirmed to have died from anthrax
before slaughter should be buried or incinerated as described, without being opened.
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b) Brucellosis (contagious abortion, Bang’s disease):
An infection caused by bacteria of the genus Brucella. Four species of Brucella cause infection in
man. These are: Br. melitensis (affecting mainly sheep and goats), Br. suis (affecting mainly
pigs), Br. abortus (affecting mainly cattle), and Br. canis (which mainly affects dogs). Animals
acquire the disease by sexual transmission or ingestion of contaminated feed. The disease causes
abortion in cattle, sheep and pigs. Goats only develop lameness and mastitis. Brucella organisms
are continuously shed in milk of lactating animals, uterine exudates, aborted fetuses and fetal
membranes of aborting animals.
Ante mortem and postmortem findings:
In cattle
Abortion in non-vaccinated pregnant cows in the last 3 - 4 months of pregnancy
Occasional inflammation of testes and epididymis
Swelling of scrotum (one or both sacs)
Edematous placenta and fetus
Hygroma on the knees
In sheep
Fever, increased respiration and depression
Inferior quality of semen in rams
Edema and swelling of scrotum
In chronic stage enlarged and hard epididymis, thickened scrotal tunics and frequently
atrophic testicles
Infertility in rams and abortion in ewes
Judgment:
Cattle carcass is approved (after removal of affected parts),
In acute abortive form (after the miscarriage), cattle carcasses are
condemned.
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Figure 46. Hygroma on the knee joints. This condition may be a sequel to Brucella abortus
infection.
Control in animals
Slaughtering of serologically positive animals and vaccination of young ones
Vaccination of all the animals in a region or country
Implementation of effective quarantine to restrict animal movement during outbreaks.
Hygienic disposal of aborted fetuses, placentae and uterine materials thereafter
disinfection of contaminated areas.
All animals introduced in a farm should be isolated and tested before joining the rest of
the herd.
Prevention of human infection
Pasteurization of milk and proper cooking of meat before consumption.
Observe personal hygiene and environmental sanitation during and after milking.
Preparation of ready-to-eat dairy products using pasteurized milk.
Ensure proper dressing and disinfection of wounds especially those on the hands that
may come into contact with brucella organisms.
Veterinarians should take special care when handling vaccines and treating infected
animals.
Rubber gloves and long sleeved gauntlets should be worn especially when removing
retained afterbirths or handling aborted fetuses.
Employees of the meat industry should wear protective clothing, treat and dress all
wounds on hands and arms.
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c) Tuberculosis
Tuberculosis is a communicable disease of worldwide occurrence that presents itself with a
respiratory problem in all animals including man. The disease is caused by organisms of the
genus Mycobacterium. The main species are: M. Tuberculosis (human TB), M. bovis (Bovine
TB) which is zoonotic), M. microti, M. avium Two major types of tuberculosis syndromes are
recognized: (1). Pulmonary tuberculosis (PTB) and (2) Extra-pulmonary TB. Epidemiology of
human TB shows increasing incidence in both developing and developed world. This is due to
increasing population density in poor areas and HIV pandemic. TB in cattle leads to infection of
various body organs including the mammary glands which makes infected milking cows to shed
mycobacteria organisms in milk urine, uterine secretions, faeces and sputum. The figure 3.1
below shows multiple tubercles on the ribs and diaphragm of a bovine infected with tuberculosis.
Transmission of zoonotic tuberculosis. Humans can acquire the disease from animal sources
through:
Consumption of unpasteurized milk from infected milking animals
Ingestion of raw and undercooked meat from infected animals
Inhalation of contaminated aerosols
Direct contact with materials contaminated with nose and mouth secretions of
infected animals
Postmortem examination of infected carcasses.
Ante mortem findings:
Low grade fever
Chronic intermittent hacking cough and associated pneumonia
Difficult breathing
Weakness and loss of appetite
Emaciation
Swelling superficial body lymph nodes
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Postmortem findings:
Tuberculosis granuloma in the lymph nodes of the head, lungs, intestine and carcass. These have
usually a well-defined capsule enclosing a caseous mass with a calcified center. They are usually
yellow in color in cattle, white in buffaloes and greyish white in other animals. Active lesions
may have a reddened periphery and caseous mass in the center of a lymph node.
Inactive lesions may be calcified and encapsulated
Nodules on the pleura and peritoneum
Lesions in the lungs , liver, spleen, kidney
Bronchopneumonia
Firmer and enlarged udder, particularly rear quarters
Lesions in the meninges, bone marrow and joints
Figure 47. A. Multiple tubercles attached to the diaphragm of a bovine carcass; B. Multiple
tubercles attached onto the ribs of a bovine carcass.
Judgment: Carcass of an animal affected with tuberculosis requires additional
postmortem examination of the lymph nodes, joints, bones and meninges.
Carcasses are condemned
Control In human:
General health promotion
Active immunization with BCG vaccine
Drug treatment
Control of animal reservoirs
Early diagnosis/treatment
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Rehabilitation
Surveillance
Control In animals
Maintain TB free herds
Applying a test and slaughter policy: Test and slaughter positive reactors
Pasteurization/boiling of milk before consumption
Proper meat inspection to identify, isolate and condemn TB cases
Proper cooking of meat before consumption
FMD is an acute viral and extremely contagious disease of cloven footed animals such as
cattle, sheep, goats, pigs and antelope.
Ante mortem findings:
Before vesicle formation:
Incubation is 1 - 5 days or Smacking and quivering of
longer lips
Morbidity: Nearly 100 % Extensive salivation and
Mortality: variable (50 % in drooling
young animals, 5 % in Shaking of feet and
adults) lameness
Fever up to 41.7°C Uneasiness and muscle
Dullness tremors After vesicle
Lack of appetite formation
This is an acute infectious viral disease of the central nervous system in mammals.
Ante mortem findings:
Furious form
Incubation from 2 weeks to 6 months or longer
Restlessness
Aggressive, may attack other animals
Sexual excitement
Bellowing
Paralysis and death
Paralytic form
Sagging and swaying of the hind quarters
Drooling and salivation
The tail is held to one side
Tenesmus or paralysis of the anus
Paralysis
The animal falls to the ground
Postmortem findings: Possible inflammation of gastrointestinal mucosa
Judgment:
In endemic areas carcasses may be approved if the animal was bitten eight
days before slaughter and within 48 hours of slaughter.
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The bite area and surrounding tissue must be condemned, and prevention
taken to prevent occupational hazards.
c) Rift Valley Fever (RVF, infectious enzootic hepatitis of cattle and sheep):
A highly contagious acute infectious viral disease of ruminants (mostly sheep), cattle and goats
and man transmitted by mosquitoes in which focal hepatic necrosis is a prominent feature. It
causes a significant high mortality in lambs. It is a notifiable disease.
Ante-mortem findings: special symptoms are absent. In sheep it is characterized by
High morbidity and mortality particularly in young lambs, rapid course, fever,
diarrhea, nasal discharge.
Abortion in pregnant ewes
Lambs usually die within 24 hours of the onset of symptoms
In cattle, the disease is less sever and less fatal, abortion in pregnant cows
High mortality in calves.
Post-mortem lesions:
Cyanotic visible mucosa
Necrosis of liver in lambs (liver may be mottled grey, or reddish brown to bright yellow
colored)
Edematous and hemorrhagic gall bladder
Hemorrhage of GIT, serosa, internal organs and lymph nodes.
Partial erosion of ileum, caecum and colon
Fetal hemorrhage, haemothorax
Judgment: -
Total condemnation of the carcass and viscera
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Self-Check 1 – Written Test
Name ID _ Date
Directions: Answer all the questions listed below. Examples may be necessary to
aid some explanations/answers.
Test I. Write true if the statement is correct/False if it is incorrect for the following
questions (5 point each).
1. Icterus is the result of an abnormal accumulation of bile pigment, bilirubin, or hemoglobin
in the blood tha leads to condemnation of carcass/offals.
2. Incomplete bleeding can be caused by stress, ineffective stunning techniques or an
ineffective bleeding cut.
3. Carcass of an animal affected with tuberculosis requires additional postmortem
examination of the lymph nodes, joints, bones and meninges
Test II. Choose the best answer for the following questions (5 point).
1. Which of the following diseases is characterized by showing abdominal swelling due to
presence of cysts in the lungs, liver or any abdominal organ in human?
A. Hydatidosis
B. Tuberculosis
C. Anthrax
D. Contagious bovine plueropnuemonia
Test III. Short Answer Questions (20 point)
a. What are the pathological conditions found at post mortem inspection?
Describe and discuss their judgement.
b. .Write the post mortem finding for the f following listed diseases.
1. Contagious bovine pleura pneumonia (CBPP):
2. Anthrax
3. Rabies
4. Rift valley fever
5. Hydatidosis
Note: Satisfactory rating - 20 points Unsatisfactory - below 20 points
You can ask your teacher for the copy of correct answers
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Information Sheet 2- Explaining effect of conducting slaughter in unhygienic
area
2.1. Introduction
Fresh meats have been implicated for number of meat borne infections and intoxications in
several countries. Microbial population that comes in contact with fresh meat during
slaughtering, dressing and processing presents a challenging problem to the meat industries.
Meat is a major source of protein consumed all over the world, it has high nutrient and water
composition, and therefore, it should be kept clean and free from microorganisms that can be
detrimental to our health. Microbial contamination can reduce the quality of fresh meat, shorten
its shelf-life and cause economic losses and health hazards.
The most important issue in all meat-processing plants is maintenance of proper hygiene and
adequate sanitary conditions. At most municipal abattoir, ante mortem examination is nil as
animals are offloaded and conveyed straight to the slaughter halls. The multiplicity of
contamination sources and the variability in facilities and practices of slaughtering and
processing operations may lead to variations in types of microorganisms and, especially, extent
of contamination introduced in meat and poultry, which may also vary with individual herds or
flocks, animals or birds, geographic location and season of the year.
Microorganisms are introduced through a variety of sources when the sterile muscles of healthy
animals and birds are exposed to the environment during slaughter, cutting and further handling.
These sources include
Contaminated air,
Contaminated water,
Contaminated soil,
Feces,
Contaminated feed,
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Contaminated hides, hair, feathers, wool, intestines, and lymph nodes,
processing equipment, utensils and humans.
Consequences of conducting slaughter in unhygienic area
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Self-Check 2 – Written Test
Name ID Date
Directions: Answer all the questions listed below. Examples may be necessary to
aid some explanations/answers.
Test I. Write true if the statement is correct/False if it is incorrect for the following
questions (5 point each).
1. Microbial population that comes in contact with fresh meat during slaughtering, dressing
and processing presents a challenging problem to the meat industries.
2. Conducting animal slaughter in unhyienic enviroment does not affect the quality and
production period of the process.
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Information Sheet 3- Assuring meat quality and hygiene maintenance
Introduction
1 Intrinsic factors: These are factors that are inherent in the food which affect microbial
growth. They include nutrient content of the food, water content of food (also called water – aw),
pH, moisture content, oxidation reduction potential, antimicrobial substances and biological
structures.
Nutrients content of the food
pH of the food
Available water in foods (water activity)
ii). Extrinsic factors:
These are factors external to the food that affect microbial growth. They include temperature of
storage, humidity of storage environment, presence and concentration of gases.
Oxygen requirements
Temperature
Relative humidity
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Food spoilage leads to:
Wastage of large quantities of food
Economic losses
Health problem
Food preservation
Food preservation is a process of treating and handling food to stop or slow down spoilage and
thus allow for longer period of storage. Food preservation aims at
Preventing growth of bacteria, yeast and molds and other microorganisms
Preventing oxidation of fats which cause rancidity
Arresting enzymatic deterioration of food
Preserving the nutritional value, texture and flavour of food.
Protecting food against attack by pests during storage.
Preservation principles and methods
Two general principles are employed in food preservation.
Inhibition principle
Killing principle,
1 Inhibition principle. In this principle, food preservation is achieved by inhibition of growth
and multiplication of microorganisms. The inhibition principle can be achieved by any of the
following methods:
Reduction in available water e.g. by drying and salting
Reduction in pH e.g by fermentation and addition of acids.
Use of preservatives, e.g sodium benzoate
Use of low temperatures (chilling or freezing)
Smoking – which has a drying and preservative effect
Preservation by these methods does not necessarily imply the destruction of microorganisms.
This is because, on removal of the inhibiting influence, the food will undergo spoilage.
Food preservation by lowering pH. Many food products can be preserved by lowering pH
so that the growth of spoilage and pathogenic bacteria is prevented. The lowering of pH can be
achieved by addition of acids and fermentation. The inhibitory effect of acids
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are exerted whether the acid is added directly to food, is a constituent of the food, or is produced
in the food by fermentation.
Food preservation by drying. Drying is the most widely used method of food preservation.
Some foods e.g grains are sufficiently dry as harvested, or with little drying can remain unspoiled
for long periods under proper storage conditions. Dry foods store for a long time at room
temperature under low humidity. Properly dried foods stored in
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moisture proof containers can stay for over one year. Moisture may be removed from foods by
means of the sun’s rays or the modern artificial methods. Microorganisms and parasites present
in the product before drying will survive the drying process.
1 Sun drying. Food is spread in the sun to dry. Sun drying is commonly used to dry grains,
certain fruits such as raisins, prunes, figs apricots; meat and fish. Sun drying requires large land
area and has problems of contamination from dust, insects, birds and rodents. To sun dry fish,
the fish scales and offal have to be removed before the fish are spread out in the sun to dry on
raised rack or clean sacks or mats. On the other hand meat is cut into thin long strips or slices
and then hang outdoors to dry.
2 Mechanical driers. Mechanical driers are used to produce dehydrated or desiccated food
product. A dehydrated or desiccated food has been dried by artificially produced heat under
controlled conditions of temperature, relative humidity and air flow.
Food preservation by use of low temperatures. Two methods are employed to arrest
microbial growth and multiplication. These are chilling (cold storage) and freezing. Chilling is
keeping food at temperatures between 0-15oC. The common chilling
temperatures ranges between 4-5oC. Temperatures that are just above freezing point maintain
foods near their original condition for a limited time (2-4 days). Chilling alone is not considered
as a method of food preservation, but a method to prolong the shelf-life of fresh products.
Freezing is keeping food at temperatures between 0oC and -35oC. However, because of the
content of electrolyte, most foods will start to freeze at -2oC to
-5oC. Low temperatures are used to retard chemical reactions and actions of food enzymes and
to slow down or stop the growth and activity of microorganisms in the food. A low enough
temperature will prevent the growth of any microorganisms.
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Impregnation of preservative chemicals from the smoke on the surface of food,
Drying effect of smoke reduces the water activity of the food,
Heat of smoking may also destroy microorganisms.
The smoke can be produced in certain smoke generators and the smoke conducted into the
smoking room or cabinets. Wood smoke contains a large number of volatile compounds that
differ in their micro biocidal or micro biostatic activities
Use of preservatives: Preservatives are physical or chemical substances used to prolong the
shelf-life of a food by protecting against deterioration caused by microorganisms. Chemical
preservatives for use in food preservation must be
Non- toxic and non-carcinogenic to mammals,
Should not react with other substances to produce toxic substances and
Should not restore a bright colour or freshness of already spoiled food product.
A good preservative should have a good inhibitory power against a wide range of
microorganisms. The use of preservatives should embrace the principle of good manufacturing
practices, where only a minimum level to achieve the desired keeping quality is used.
Preservatives can be grouped into:
2 Killing principle: In this principle, spoilage microorganisms are destroyed in the
food and thereafter the food is protected against subsequent contamination by being enclosed
in an air tight container. This principle is achieved by heating: Two methods are used to
preserve food by heat treatment. These are: (i). Pasteurization, and (ii) sterilization.
Pasteurization. Is a process of heat treatment of a food substance at specific
temperatures and times, aimed at destroying all pathogenic microorganisms except spores,
without affecting the nutritive quality of the food. Pasteurization leads to destruction of 99% of
all vegetative organisms and all pathogenic microorganisms. Pasteurization is commonly
employed in the milk industry. Three methods are usually employed:
Low temperature, long time (63oC for 30 minutes)
High temperature, short time (72oC for 15 seconds)
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Flash method at 80oC for 1-2 seconds/or continuously
Sterilization. Is a physical or chemical process which destroys or eliminates all forms of life
especially microorganisms from a given substance such as food. Sterilization can be achieved by
heating food at temperatures above 140oC. Some of the foods preserved by sterilization include
ultra heat treated milk and canned meat. During sterilization, some food nutrients are destroyed
such as vitamin C. However, sterile foods store for a long time. Such foods only spoil through
occurrence of chemical reactions in the stored food leading to fat rancidity and off-flavours.
Canning is the process of heating the product at a specified temperature for a specific length of
time (pasteurizing), and then vacuum sealing the pasteurized food in special glass jars designed
for this purpose. It can be used with most foods, including fruits, vegetables, meats, seafood, and
some prepared foods. Canning requires the purchase of reusable canning jars and rings, one-time
use sealing lids, and some practice to learn the necessary and detailed steps.
Purpose of Canning: means rendering meat sterile by packing it in hermetically
To produce meat with long shelf life by
Creating unfavorable condition for microbial growth.
Destruction of microorganisms.
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Self-Check 3 – Written Test
Name ID Date
Directions: Answer all the questions listed below. Examples may be necessary to
aid some explanations/answers.
Test I. Write true if the statement is correct/False if it is incorrect for the following
questions (6 point each).
1. Food spoilage is the decomposition of food components resulting in change in quality,
appearance, colour, flavour, texture and odor.
2. Canning is the process of heating the product at a specified temperature for a specific length
of time (pasteurizing), and then vacuum sealing the pasteurized food in special glass jars
Test II. Choose the best answer for the following questions (3 point each).
1. Food spoilage result from:
A. Growth and activity of spoilage microorganisms
B. Action of enzymes of plant or animal tissues
C. Chemical reactions of non-enzymatic nature
D. All of the above
2. Which one of the following is is incorrect regarding food pasteurization process?
A. Low temperature, long time (63oC for 30 minutes)
B. High temperature, short time (78oC for 15 seconds)
C. Flash method at 80oC for 1-2 seconds/or continuously
D. All of the above
Test III. Short Answer Questions (10 point)
1. Describe and discuss principles which are employed in food preservation.
2. What is food preservation by use of low temperatures?
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Information Sheet 4- Describing risk of consuming raw meat and meat products
Introduction
The consumption of raw foods/meat or uncooked foods may probably give some effects or
implications to human health; whether the effects are beneficial or dangerous to human.
According to science, eating raw foods has its own pros and cons. Generally, some people
nowadays prefer a raw foods diet because of the potential health benefits and advantages. This is
due to the people awareness to protect their health as they believed that raw foods will give the
positive effect. According to the former research that has been made, raw foods have less
processing and fewer added ingredients. Thus, this is a great advantage and benefit to the person
who choose this kind of raw foods as their dishes.
Another benefit that can be found through consuming raw foods is that the enzymes contained in
those foods can be preserved. Enzymes are defined as the protein molecules which is found in
plants and also made by the human body. The act of cooking the foods is believed to destroy the
enzymes found in it. Generally, many of the people who support raw foods diet believe there are
health benefits of eating foods with the preserved enzymes.
There is scientific research which proved that consuming raw foods, i.e. the raw meat may give
negative impacts on human health. Factually, a large body of research has been conducted on the
high risk for pathogenic bacterial contamination of raw meat diets and the potential risks posed
by this problem. Like any raw meat products we encounter at home or in restaurants, raw meat
diets actually have the potential to carry pathogenic bacteria or dangerous microorganism.
Some of those bacteria are unlikely to have negative effects on health, but others can have
serious consequences if they were transmitted to human.
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Example of bacteria that may exist when people consume raw meat in their diets
Escherichia coli (E. coli). E. coli is usually found mainly on the surface of the meat.
Salmonella
Listeria
Moreover, eating raw meat may cause a person to suffer from other food-borne illness.
The other bacteria can potentially contaminate beef and cause food poisoning if the meat
is eaten raw. Viruses, such as the hepatitis A virus can also be contracted from
contaminated and raw meat.
Besides, consuming undercooked or raw beef or meat also increases the risk for being
infected by parasitic infections, such as beef tapeworm.
Therefore, it can be concluded that consuming or eating raw meat will expose a person or an
individual to the health risks. This is because raw meat contains a lot of dangerous bacteria that
will affect our health. Those bacteria are very dangerous and people should avoid themselves
from being infected by them. Avoid from eating raw meat and it is better if people nowadays
consume cooked meat rather than the raw meat. Once people suffer from infection, there is a
probability to be infected with other illness. Thus, a person should prevent himself or herself
from including the raw meat in his or her diet.
Page 156 of 185 Holeta PTC TVET program title- Animal Health Care Service Version -1
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Self-Check 4 – Written Test
Name ID Date
Directions: Answer all the questions listed below. Examples may be necessary to
aid some explanations/answers.
Test I. Write true if the statement is correct/False if it is incorrect for the following
questions (4 point each).
1. Consuming undercooked or raw beef or meat ncreases the risk for being infected by
parasitic infections.
Test II. Choose the best answer for the following questions (4 point).
1. Which one of the following bacteria that may exist when people consume raw meat in their
diets?
A. Escherichia coli (E. coli). E. coli)
B. Salmonella
C. Listeria
D. All of the above
Page 157 of 185 Holeta PTC TVET program title- Animal Health Care Service Version -1
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Information Sheet 5- Undertaking disposal of part of carcass or organs found
unfit
Introduction
Disposal of slaughter waste is a problem in many African countries. It is not uncommon to find
slaughterhouse wastes including carcass trimmings, horns, hooves, bones, and manure
accumulating in the vicinity of the slaughter facility causing environmental contamination and
attraction of pests and scavengers.
Parts of carcass and organs unfit
Blood
Male/female reproductive organs including lactating udders/penis
Gall bladder
Bladder.
Carcasses and portions of meat condemned by the meat inspector/veterinarian which
poses a possible health threat. Such material must be held under secure conditions until
disposed of in accordance with legislation.
Crop in case of chickens
Rectum/cloaca in chickens
The wastes from a slaughter house are usually heavily polluted. Therefore, waste water from a
slaughter house should not be allowed directly into a municipal drainage system without
previous treatment. In the slaughter premises, the general principles regarding waste disposal
are;
Page 158 of 185 Holeta PTC TVET program title- Animal Health Care Service Version -1
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The solid waste (e.g. sweepings from the floor of the dressing area etc.) must be
separated from its liquid counterpart.
Secondly, the liquid waste must be separated from the conventional drainage system
of the toilets and bathrooms.
The two lines should be separate and be identified before being joined together on the
outside.
The purpose of this is to prevent contamination of the premises in the event of a back- up of
conventional sewage in the early stages of discharge. A trap gully basin should be provided to
collect residual solids, (especially fat) to prevent clogging of the system. Clogging can also result
from discharge and coagulation of blood in the drainage system creating further back-up
problems. It is therefore better to collect blood in special containers for disposal. The second
phase of treatment involves bacterial breakdown of dissolved substances in the liquid phase. This
process requires oxygen to convert organic matter into simple inorganic substances, which are
removed by physical treatment or by chemical means. Only at this stage the water is considered
treated, although it is not recommended for human use. This water can be used for agricultural
purposes or be discharged into the sea, rivers or streams.
Page 159 of 185 Holeta PTC TVET program title- Animal Health Care Service Version -1
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Slashing and then spraying with, or immersion in, an obnoxious colorant
approved for the purpose; and
Burial (After the site has been approved by Department of Environmental Affairs) and
immediate covering to a depth of at least 60 cm and not less than 100 m from the
abattoir, providing such material may not deleteriously affect the hygiene of the
abattoir; or
Processing at a registered sterilizing plant.
With special approval from the Provincial Executive Officer of Veterinary Services
certain of the condemned material can be made available for carnivorous animals,
crocodiles and for vulture restaurants.
Manure/Waste Disposal
Digestive and excretory wastes of ruminants, collectively referred to as manure, are a mixture of
dung and urine and occur in two forms:
As sweepings from lairages which are built into heaps outside the slaughter building and
collected from time to time in small quantities by small farmers to be used as fertilizer.
And as kraal manure which is less preferred, as it involves manure remaining permanently
on the holding grounds, which may create problems of collection.
Manure is usually utilized for composting but can also be used for biogas (fuel)
production.
Page 160 of 185 Holeta PTC TVET program title- Animal Health Care Service Version -1
Author/Copyright Level - 4 September, 2021
Self-Check 5 – Written Test
Name ID Date
Directions: Answer all the questions listed below. Examples may be necessary to
aid some explanations/answers.
Test I. Write true if the statement is correct/False if it is incorrect for the following
questions (5 point each).
1. Carcasses and portions of meat condemned by the meat inspector/veterinarian which
poses a possible health threat.
Page 161 of 185 Holeta PTC TVET program title- Animal Health Care Service Version -1
Author/Copyright Level - 4 September, 2021
Reference Materials
Book References
Page 162 of 185 Holeta PTC TVET program title- Animal Health Care Service Version -1
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Internet References
1. https://www.bing.com/newtabredir?url=%2Fimages%2Fsearch%3Fq%3Dzoonosis
%26id%3D6F4827BD367BF8BD292F6728238282458DBA8015%26FORM%3DIQ
FRBA
2. Future trends in Veterinary public health Report of WHO 2002. http://www.oie.int
3. A Field Manual for Collection of Specimens to Enhance Diagnosis of Animal
Diseases
4. Collection and shipment of diagnostic specimens
https://www.oie.int/fileadmin/Home/eng/Animal_Health_in_the_World/docs/pdf/1.1.
01_COLLECTION.pdf
5. Carcass inspection and preparation https://www.bestpracticeguides.org.uk/wp-
content/downloads/carcass_inspect.pdf
6. https://www.menshealth.com/health/a28007348/eating-raw-meat-safety/
7. Impact of unhygienic condition during slaughtering
https://www.bing.com/search?q=Explaining+effect+of+conducting+slaughter+in+un
hygienic+area+&form=ANNTH1&refig=9425331a3c264430aa1a0e1089517357
8. Community awareness and participation plan (CAPP)
https://www.adb.org/sites/default/files/linked-documents/43524-014-nep-oth-01.pdf
9. Current status of veterinary public health activities in Bangladesh and its future plans
https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-019-1879-8
10. Raw food consumption And Its effects on human Health: An Analysis Of Halalan
Toyyiban Concept
https://mail.google.com/mail/u/0/#inbox/CllgCKCDBwBdMMLWdcpJTfXKJzcnwxqT
sBMbZwphhgMwszGtZJPCNNrqhZXlrZCkzPxPmDbRNWg?projector=1&message
PartId=0.6
11. Carcasses and meat cuts —Specification https://mail.google.com/mail/u/0/?
tab=km#inbox/GTvVlcSHwfVlRQtVbhTWqmbhL
QqdQKDDSGKrdlLcfWbmdxQVkSTkBhjjgJgVVDggdTwzBBnNzBpst?projector=1
&messagePartId=0.1
12. Procedures and equipment for specimen collection
Page 163 of 185 Holeta PTC TVET program title- Animal Health Care Service Version -1
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https://mail.google.com/mail/u/0/?tab=km#inbox/DmwnWsLWPKphznSMcfKNjlTG
nLPKvMzrVCqQHStTPZTTlQcWHxfprdpJPptHPgmwhvBsmgrZQWVQ?projector=
1&messagePartId=0.1
13. SPECIMEN PACKAGING, MARKING and LABELLING AND DOCUMENTATION
instructionshttps://mail.google.com/mail/u/0/?tab=km#inbox/DmwnWsmHbfTvhrHX
WpncSdMWcqQwxrnDCfRdwwBpdXfsrMWrQNjSzgBKqPbJzDnRvHFvmNhgmdL B?
projector=1&messagePartId=0.1.
14. Guidelines on the Organiza2on of Samples in a Laboratory
https://mail.google.com/mail/u/0/?tab=km#inbox/CllgCJlFlbgbRwvtcpkdtWnWlGn
wgZMKdQGFbvtWrVhcDBTrGjPcprdQnLDLvHQDpCNwQvdnvxV?projector=1&m
essagePartId=0.1
15. Training manual for hygienic and sanitary slaughter of small Ruminants
https://mail.google.com/mail/u/0/?tab=km#inbox/CllgCJlHFDmQrXSXqmMdlVGVX
pLWzPddNfbHWNcNfVLQdVxJQxMpghMtWjPMNRPHWKklfVQkVKL?projector=1
&messagePartId=0.1
16. Creating health awareness: a social media enabled collaboration
creating_awareness_through_social_media_-_health_and_technology_0.pdf (mcgill.ca)
Page 164 of 185 Holeta PTC TVET program title- Animal Health Care Service Version -1
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AKNOWLEDGEMENT
We wish to extend thanks and appreciation to the many representatives of TVET instructors and
respective industry experts who donated their time and expertise to the development of this
Teaching, Training and Learning Materials (TTLM).
We would like also to express our appreciation to the TVET instructors and respective industry
experts of Oromia Regional State TVET Bureau, Holeta polytechnic College and the World
Bank who made the development of this Teaching, Training and Learning Materials (TTLM)
with required standards and quality possible.
The Teaching, Training and Learning Materials (TTLM) was developed on September 2021 at
Adama, Pan Africa Hotel.
Page 165 of 185 Holeta PTC TVET program title- Animal Health Care Service Version -1
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The trainers who developed the learning guide
No Name Qualification Educational College Mobile Phone E-mail
Page 166 of 185 Holeta PTC TVET program title- Animal Health Care Service Version -1
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