Explain What Hypotonic, Hypertonic and Isotonic Solutions Are
Explain What Hypotonic, Hypertonic and Isotonic Solutions Are
Explain What Hypotonic, Hypertonic and Isotonic Solutions Are
Hypotonic, Hypertonic
and Isotonic solutions
In this subtopic, we mostly
COMPARING between two
concentration..so don’t forget
to compare …
ISOTONIC
• SolutionA in
Solution is which theto
isotonic
concentrations of solute
solution B are equal
0.5 M 0.5 M
sucrose sucrose
solution solution
HYPERTONIC
• The
Solution B iswith
solution hypertonic
a higher
compared
solute to solution A
concentration
Explain plasmolysis,
deplasmolysis, haemolysis and
crenation
4 points to be remembered…
1. Compare concentration(A-B)
2. diffusion–water (A-B)
3. By osmosis(through plasma
membrane)
4. Result –shape (cell)
4 points to be remembered…
1. A is _______compare to B
2. Water diffuse from _?_ to _?__..
3. By osmosis(through plasma
membrane)
4. The cell become______.
Animal and plant cells in isotonic
solution
rbc are isotonic to 0.85% or 0.15 The cell sap of onion cells
M of NaCl solution are isotonic to 0.5-0.55 M or
rbc are also isotonic to 0.28 M 17%-18% of sucrose solution
glucose solution
The 0.15 M NaCl solution is isotonic to the
cytoplasmic fluid of red blood cell (rbc)
0.15 M NaCl
solution
Equal to
Concentration of
Rbc cytoplasmic
fluid.
ISOTONIC
Water
diffuses into
of the cell at
equal rates by
osmosis
And out
ISOTONIC
There is no
net movement
of water across
the plasma
membrane (no
net gain or net
loss of water)
0.5 M sucrose
solution
Equal to
Concentration of
the cell sap of
onion cell
ISOTONIC
Water
diffuses into
of the cell at
equal rates by
osmosis
And out
ISOTONIC
There is no
net movement
of water across
the plasma
membrane (no
net gain or net
loss of water)
Hypertonic
to
Rbc cytoplasmic
fluid.
Low conc.
Water
diffuses out
of the cell
By osmosis
through
plasma
The cells shrivel
membrane
and the plasma
membrane
crinkles up
The cells have undergone
CRENATION
Remember this :
CRENATION is
the process
Normal
RBC Immersed in hypertonic solution
The result is
cell become
CRENATED/
SHRINK/
SHRIVEL
Plant cells in hypertonic solution
1.0M sucrose
solution
Higher conc.
Hypertonic
to
Cell sap.
Low conc.
Water
diffuses out
of the
vacuole
By osmosis
Both the
vacuole and
cytoplasm
shrink
• The plasma
membrane
pulls away
from the cell
wall
PLASMOLYSI
S is the
process
Immersed in hypertonic solution
cell become
PLASMOLYSE
D/ FLACCID
Animal cells in hypotonic solution
Distilled water
low conc.
Hyp0tonic
to
Rbc cytoplasmic
fluid.
high conc.
Water
diffuses into
the cell
By osmosis
through
plasma
The cells swell
membrane
up and burst
(haemolysis)
Remember this :
HAEMOLYSIS
is the process
Immersed in hypotonic solution
cell become
the plasma membrane HAEMOLYSED
ruptures and the content / SWELL UP
are released into the AND BURST
surrounding
Plant cells in HYPOtonic
solution
Distilled water
low conc.
Hyp0tonic
to
Cell sap
high conc.
Water
diffuses into
the cell
By osmosis
through
plasma
membrane
The cells
become turgid
• Distilled water is hypotonic to the cell
sap of onion cell
• Water diffuses into the cells by
osmosis
• The vacoule expands causing the cells
to swell
• The vacuole and cytoplasm press
outwards against the plasma
membrane
• The cells become turgid
The swelling of a plant cell in hypotonic solution creates
Turgor Pressure within the cell. This pressure prevents
the cell from taking in too much water and bursting.
Hypotonic Isotonic Hypertonic
solution solution solution
Plant cell P: P: P: plamolysis