Explain What Hypotonic, Hypertonic and Isotonic Solutions Are

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Explain what hypotonic,

hypertonic and isotonic


solutions are

Hypotonic, Hypertonic
and Isotonic solutions
In this subtopic, we mostly
COMPARING between two
concentration..so don’t forget
to compare …
ISOTONIC
• SolutionA in
Solution is which theto
isotonic
concentrations of solute
solution B are equal

0.5 M 0.5 M
sucrose sucrose
solution solution
HYPERTONIC
• The
Solution B iswith
solution hypertonic
a higher
compared
solute to solution A
concentration

0.5 M sucrose 1.0 M sucrose


solution solution
HYPOTONIC
Solution
• The A iswith
solution hypotonic
lower
compared
solute to solution B
concentration

0.5 M sucrose 1.0 M sucrose


solution solution
Explain the effects of
hypotonic, hypertonic and
isotonic solutions on plant cell
and animal cell

Explain plasmolysis,
deplasmolysis, haemolysis and
crenation
4 points to be remembered…

1. Compare concentration(A-B)
2. diffusion–water (A-B)
3. By osmosis(through plasma
membrane)
4. Result –shape (cell)
4 points to be remembered…

1. A is _______compare to B
2. Water diffuse from _?_ to _?__..
3. By osmosis(through plasma
membrane)
4. The cell become______.
Animal and plant cells in isotonic
solution

rbc are isotonic to 0.85% or 0.15 The cell sap of onion cells
M of NaCl solution are isotonic to 0.5-0.55 M or
rbc are also isotonic to 0.28 M 17%-18% of sucrose solution
glucose solution
The 0.15 M NaCl solution is isotonic to the
cytoplasmic fluid of red blood cell (rbc)

0.15 M NaCl
solution

Equal to

Concentration of
Rbc cytoplasmic
fluid.
ISOTONIC
Water
diffuses into

of the cell at
equal rates by
osmosis

And out
ISOTONIC

There is no
net movement
of water across
the plasma
membrane (no
net gain or net
loss of water)

The cells retain their normal shape


The 0.5 M sucrose solution is isotonic to the
cell sap of onion cell

0.5 M sucrose
solution

Equal to

Concentration of
the cell sap of
onion cell
ISOTONIC
Water
diffuses into

of the cell at
equal rates by
osmosis

And out
ISOTONIC

There is no
net movement
of water across
the plasma
membrane (no
net gain or net
loss of water)

The cells retain their normal shape


Animal cells in hypertonic solution

Crenated rbc Normal rbc


0.5 M NaCl solution
Higher conc.

Hypertonic
to

Rbc cytoplasmic
fluid.
Low conc.
Water
diffuses out
of the cell
By osmosis
through
plasma
The cells shrivel
membrane
and the plasma
membrane
crinkles up
The cells have undergone
CRENATION
Remember this :

CRENATION is
the process
Normal
RBC Immersed in hypertonic solution

The result is
cell become
CRENATED/
SHRINK/
SHRIVEL
Plant cells in hypertonic solution
1.0M sucrose
solution
Higher conc.
Hypertonic
to

Cell sap.
Low conc.
Water
diffuses out
of the
vacuole
By osmosis
Both the
vacuole and
cytoplasm
shrink
• The plasma
membrane
pulls away
from the cell
wall

The plant cells have


plasmolysed, becomes flaccid
and less turgid
Remember this :

PLASMOLYSI
S is the
process
Immersed in hypertonic solution

cell become
PLASMOLYSE
D/ FLACCID
Animal cells in hypotonic solution
Distilled water
low conc.

Hyp0tonic
to

Rbc cytoplasmic
fluid.
high conc.
Water
diffuses into
the cell
By osmosis
through
plasma
The cells swell
membrane
up and burst
(haemolysis)
Remember this :

HAEMOLYSIS
is the process
Immersed in hypotonic solution

cell become
the plasma membrane HAEMOLYSED
ruptures and the content / SWELL UP
are released into the AND BURST
surrounding
Plant cells in HYPOtonic
solution
Distilled water
low conc.

Hyp0tonic
to

Cell sap
high conc.
Water
diffuses into
the cell
By osmosis
through
plasma
membrane
The cells
become turgid
• Distilled water is hypotonic to the cell
sap of onion cell
• Water diffuses into the cells by
osmosis
• The vacoule expands causing the cells
to swell
• The vacuole and cytoplasm press
outwards against the plasma
membrane
• The cells become turgid
The swelling of a plant cell in hypotonic solution creates
Turgor Pressure within the cell. This pressure prevents
the cell from taking in too much water and bursting.
Hypotonic Isotonic Hypertonic
solution solution solution
Plant cell P: P: P: plamolysis

C: turgid C: remain the C: plasmolysed,


same flaccid

Animal cell P: haemolysis P: P: crenation

C: haemolysed, C: remain the C: crenated


burst, shape shrivel,
swell up crinkles up
Movement Water diffuse into Water diffuse Water diffuse out
of water the cell by into and out of from the cell by
osmosis through the cell at equal osmosis through
plasma rate by osmosis plasma membrane
membrane
The Effects and Application of
Osmosis in everyday Life
1. Wilting in plants
• caused by excessive use of fertilisers
• fertilisers dissolve in the soil water
• the soil water is hypertonic to the cell
sap of plant root
• water diffuses out of the cell sap by
osmosis
• causing the cells to be plasmolysed and
become flaccid
• eventually this leads to wilting
Wilting in plant
2. Food preservation
• some food can be preserved to last
longer by using natural preservatives
such as salt, sugar and vinegar
• when preservatives are added to the food,
the surrounding solution is hypertonic to
the contents of the food
• water diffuses out from the food by
osmosis
• preservatives diffuse in by simple
diffusion
• the food will become dehydrated and
the presence of preservatives prevent
the growth of fungi and bacteria that
can spoil the food
Appreciating the movement of substances
across the plasma membrane
• Important for the survival of a cell
• By being semi-permeable, the plasma
membrane is able to control what goes in
and what goes out
• The proper functioning of the plasma
membrane is very important because it
helps to bring in important substances such
as glucose (carrier protein)

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