Measurement of Human Energy Expenditure: - Understand The Following

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Measurement of Human Energy

Expenditure
• Understand the following:
– Heat of combustion (Calories)
– Atwater factors
– How to measure kCal
– Quantify energy release in metabolism
– Quantify food mixture metabolized

Professor V. Katch MVS 110: Measurement of Energy


Expenditure
What is a Calorie?
• A measurement of food energy
– Quantity of ____ necessary to raise the
temperature of 1 ___ (1 L) of water 1 degree
___ (from 14.5 to 15.5°C)
– If a food contains 300 kCal then releasing the
potential energy trapped within this food’s
chemical structure increases the temperture of
300 L of water 1°C.
– Different foods contain different amounts of
heat energy.

Professor V. Katch MVS 110: Measurement of Energy


Expenditure
How to Measure Calories?
The Bomb Calorimeter

Figure 1. Bomb calorimeter directly measures the energy value of food.

Professor V. Katch MVS 110: Measurement of Energy


Expenditure
Heat of Combustion
• The heat liberated by oxidizing (burning)
food in a bomb calorimeter represents its
_______ ____ _______ or total energy
value of the food.
– Average heats of combustion
• CHO = 4.2 kCal•g-1
• Lipid = 9.4 kCal• g-1
• Protein = 5.65 kCal•g-1

Professor V. Katch MVS 110: Measurement of Energy


Expenditure
Net Energy Value of Foods
• Gross energy value from the bomb calorimeter
are different than what happens in the body.
• Net energy values equal what is available to the
body after digestion:
– CHO =
– Lipid = Called Atwater factors
– Protein =

Professor V. Katch MVS 110: Measurement of Energy


Expenditure
Energy Value of What You Eat
• Calculate energy value of a meal
Atwater factor x food grams = total kCal
Go through an example for 100 g of food that
contains 4% protein (4 g), 13% fat (13 g) and
21% CHO (21 g).
[(4 x 4.0) + (13 x 9.0) + 21 x 4.0)]

[16 + 117 + 84 = 217 kCal

Professor V. Katch MVS 110: Measurement of Energy


Expenditure
Measuring Human kCal Production

METABOLISM CALORIMETRY

Direct Indirect

CO2 +
Oxygen Consumption N2 Balance

Open Circuit Closed Circuit

Professor V. Katch MVS 110: Measurement of Energy


Expenditure
Measuring Human kCal Production
FOODSTUFF + OXYGEN ŠŠŠŠŠ> HEAT + CO2 + H2O

INDIRECT CALORIMETRY DIRECT CALORIMETRY

MEASURE EITHER HEAT


OR O2

Professor V. Katch MVS 110: Measurement of Energy


Expenditure
Measuring Human kCal Production
Direct Calorimetry [Atwater-Rosa]

Professor V. Katch MVS 110: Measurement of Energy


Expenditure
Indirect Calorimetry
Measuring VO2 uptake
[Closed Circuit Method]

Positive:Negative Aspects

Professor V. Katch MVS 110: Measurement of Energy


Expenditure
Indirect
Calorimetry
[Measure VO2 uptake]
Open Circuit Method

Positive:Negative Aspects

Professor V. Katch MVS 110: Measurement of Energy


Expenditure
Direct v Indirect Calorimetry
• Calorific Transformation for Oxygen

– For every liter (L) of oxygen consumed, 5


kCal of energy are transformed

• Thus, indirect calorimetry is a good


measure of heat production

Professor V. Katch MVS 110: Measurement of Energy


Expenditure
What Food is Metabolized?
• The Respiratory Quotient (RQ)
– RQ =

• Every food substance has a specific RQ

Professor V. Katch MVS 110: Measurement of Energy


Expenditure
RQ for CHO
• RQ =

– C6H12O6 + 6 O2 –––––> 6 CO2 + 6 H2O

Professor V. Katch MVS 110: Measurement of Energy


Expenditure
RQ for Lipid
• RQ =

Between 0.69 and 0.73

Professor V. Katch MVS 110: Measurement of Energy


Expenditure
RQ for Protein
• RQ =

Professor V. Katch MVS 110: Measurement of Energy


Expenditure
RQ for Mixed Foods
• RQ =

Professor V. Katch MVS 110: Measurement of Energy


Expenditure
Thermal Equivalent of Oxygen
for the RQ
• RQ = 0.70 = 4.686 kCal per L O2
• RQ = 0.84 = 4.85 kCal per L O2
• RQ = 1.0 = 5.05 kCal per L O2

Professor V. Katch MVS 110: Measurement of Energy


Expenditure
Liters of Oxygen and kCals
1 Liter per min 5 kCal per min Heat
Oxygen Consumed Liberated

At Rest
250 mL per min 1.25 kCal per min
Oxygen Consumed heat liberated

Professor V. Katch MVS 110: Measurement of Energy


Expenditure

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