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    Endo Adar e

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    Endo Adar e

    Uploaded by

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    of 3

    M1077

    Endo Agar Base

    Endo Agar Base is recommended for preparing Endo Agar to confirm presumptive test for lactose fermenting coliforms.

    Composition**
    Ingredients
    Peptic digest of animal tissue
    Lactose
    Dipotassium phosphate
    Sodium sulphite
    Agar
    Final pH ( at 25C)
    **Formula adjusted, standardized to suit performance parameters

    Gms / Litre
    10.000
    10.000
    3.500
    2.500
    12.000
    7.50.2

    Directions
    Suspend 38 grams in 1000 ml distilled water. Add 4 ml of 10% Basic Fuchsin (FD059). Heat to boiling to dissolve the medium
    completely. Sterilize by autoclaving at 15 lbs pressure (121C) for 15 minutes. Mix well before pouring into sterile Petri plates.
    If the solidified culture medium is somewhat too red, then to remove the colour, add a few drops (max. 1 ml/litre) of a freshly
    prepared 10% Sodium sulphite solution and boil.
    Caution : Basic fuchsin is a potential carcinogen and care should be taken to avoid inhalation of the powdered dye and
    contamination of the skin .

    Principle And Interpretation


    Endo had first developed a culture medium for differentiation of lactose fermenters and lactose non-fermenters while inhibiting
    gram-positive bacteria (1). Inhibition of the later was achieved without the use of bile salts as was traditionally used. Endo
    was successful in inhibiting gram-positive bacteria on his medium by the incorporation of sodium sulphite and basic fuchsin.
    The resulting Endo Agar, also known as Fuchsin Sulphite and Infusion Agar, was used to isolate the typhoid bacilli. Many
    modifications of this media have been done over the years. Endo Agar is recommended by APHA as an important medium in
    the microbiological examination of water and wastewater, dairy products and foods (2-4). Endo Agar is used to confirm the
    detection and enumeration of coliform bacteria following presumptive test of drinking water. It is also used for the detection
    and isolation of coliforms and fecal coliforms from milk, dairy products and food.
    The medium contains peptic digest of animal tissue which provide nitrogen, carbon, vitamins and minerals required for bacterial
    growth. Sodium sulphite and basic fuchsin (FD) has inhibitory effect on gram-positive microorganisms. Lactose fermenting
    coliforms produce aldehyde and acid. The aldehyde in turn liberates fuchsin from the fuchsin-sulphite complex, giving rise to
    a red colouration of colonies. With Escherichia coli , this reaction is very pronounced as the fuchsin crystallizes, exhibiting
    a permanent greenish metallic lustre (fuchsin lustre) to the colonies.

    Quality Control
    Appearance
    Cream to yellow homogeneous free flowing powder
    Gelling
    Firm, comparable with 1.2% Agar gel
    Colour and Clarity of prepared medium
    After addition of FD059: Orangish pink coloured, After addition of FD059: clear to slightly opalescent gel with fine
    precipitate forms in Petri plates.
    Reaction
    Reaction of 3.8% w/v aqueous solution at 25C. pH : 7.50.2
    pH
    Please refer disclaimer Overleaf.

    HiMedia Laboratories

    Technical Data

    7.30-7.70
    Cultural Response
    Cultural characteristics observed with added Basic fuchsin (FD059) after an incubation at 35-37C for 18-24 hours.
    Cultural Response
    Organism

    Inoculum
    (CFU)

    Cultural Response
    Bacillus subtilis ATCC 6633 >=10
    Enterobacter aerogenes
    50-100
    ATCC 13048
    Enterococcus faecalis ATCC 50-100
    29212
    Escherichia coli ATCC
    50-100
    25922

    Growth

    Recovery

    Colour of
    Colony

    inhibited
    0%
    good-luxuriant >=50%

    pink

    none-poor

    pink, small

    <=10%

    good-luxuriant >=50%

    Klebsiella pneumoniae
    50-100
    ATCC 13883
    Proteus vulgaris ATCC
    50-100
    13315
    Pseudomonas aeruginosa
    50-100
    ATCC 27853
    Salmonella Typhi ATCC
    50-100
    6539
    Shigella sonnei ATCC 25931 50-100

    good-luxuriant >=50%

    Staphylococcus aureus
    >=10
    ATCC 25923
    Enterobacter cloacae ATCC 50-100
    13047
    Salmonella Typhimurium
    50-100
    ATCC 14028
    Salmonella Enteritidis ATCC 50-100
    13076
    Shigella flexneri ATCC
    50-100
    12022

    inhibited

    0%

    good

    40-50%

    good-luxuriant >=50%
    good-luxuriant >=50%
    good-luxuriant >=50%
    good-luxuriant >=50%

    pink to rose red


    with metallic
    sheen
    pink, mucoid
    colourless to
    pale pink
    colourless,
    irregular
    colourless to
    pale pink
    colourless to
    pale pink

    pink

    good-luxuriant >=50%

    colourless

    good-luxuriant >=50%

    colourless

    good-luxuriant >=50%

    colourless

    Storage and Shelf Life


    Store below 30C in tightly closed container and prepared medium at 2-8C and away from light to avoid photo oxidation.
    Use before expiry date on the label.

    Reference
    1. Endo, 1904, Zentralbl. Bakteriol., Abt. I. Orig., 35:109.
    2. Eaton A. D., Clesceri L. S., Rice E. W. and Greenberg A. W., (Eds.), 2005, Standard Methods for the Examination of Water
    and Wastewater, 21st Ed., APHA, Washington, D.C.
    3. Downes F. P. and Ito K.,(Eds.), 2001, Compendium of Methods for the Microbiological Examination of foods, 4th Ed.,
    American Public Health Association, Washington, D.C.
    4. Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,
    APHA Inc., Washington, D.C.
    Revision : 1 / 2011

    Please refer disclaimer Overleaf.

    HiMedia Laboratories

    Technical Data

    Disclaimer :
    User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
    this and other related HiMedia publications. The information contained in this publication is based on our research and development
    work and is to the best of our knowledge true and accurate. HiMedia Laboratories Pvt Ltd reserves the right to make changes to
    specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
    for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
    be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
    HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147
    1919 Email: [email protected] Website: www.himedialabs.com

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