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    Rose Bengal

    Uploaded by

    Khaza Vinod

    Copyright:

    © All Rights Reserved
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    Rose Bengal

    Uploaded by

    Khaza Vinod
    14 views3 pages

    Original Title

    rose bengal

    Copyright

    © © All Rights Reserved

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    Copyright:

    © All Rights Reserved
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    14 views3 pages

    Rose Bengal

    Uploaded by

    Khaza Vinod

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 3

    Rose Bengal Agar w/ Chlortetracycline M1584

    Rose Bengal Agar w/ Chlortetracycline is recommended for the selective isolation and enumeration of yeasts and moulds
    from environmental materials and foodstuffs.
    Composition**
    Ingredients Gms / Litre
    Peptone, special 5.000
    Dextrose 10.000
    Monopotassium phosphate 1.000
    Magnesium sulphate 0.500
    Rose bengal 0.050
    Agar 15.000
    Final pH ( at 25°C) 7.2±0.2
    **Formula adjusted, standardized to suit performance parameters

    Directions
    Suspend 31.55 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at
    15 lbs pressure (121°C) for 15 minutes. Cool to 45°C and aseptically add the rehydrated contents of 1 vial of Chlortetracycline
    Selective Supplement (FD120) to the medium. Mix thoroughly and pour into sterile Petri plates.

    Principle And Interpretation


    The use of media with an acid pH that selectively inhibits the growth of bacteria and thereby promotes the growth of fungi has
    been widely employed (1,2,3). A number of investigators have reported, however, that acidified media may actually inhibit
    fungal growth (4,5), fail to completely inhibit bacterial growth (4) and have little effect in restricting the size of mold colonies
    (6) Smith and Dawson (7) used rose bengal in a neutral pH medium for the selective isolation of fungi from soil samples.
    Chloramphenicol, streptomycin, oxytetracycline and chlortetracycline have been used for the improved, selective isolation and
    enumeration of yeasts and molds from soil, sewage and foodstuffs (5,8-11).
    Rose Bengal Agar Base is a modification of the Rose Bengal Chlortetracycline Agar formula of Jarvis (9). Instead of
    chlortetracycline, chloramphenicol is employed in this medium as a selective supplement. Of the antibiotics most frequently
    employed in media of neutral pH, Chloramphenicol is recommended because of its heat stability and broad antibacterial
    spectrum (12). Rose Bengal Agar media are recommended in standard methods for the enumeration of yeasts and molds from
    foodstuffs and water. (12-14).
    Peptone special provides the carbon and nitrogen sources required for good growth of a wide variety of organisms. Dextrose
    is an energy source. Monopotassium phosphate provides buffering capability. Magnesium sulfate provides necessary trace
    elements. Rose bengal is included as a selective agent that inhibits bacterial growth and restricts the size and height of
    colonies of the more rapidly growing molds. The restriction in growth of molds aids in the isolation of slow-growing fungi
    by preventing overgrowth by more rapidly growing species. Rose bengal is taken up by yeast and mold colonies, thereby
    facilitating their recognition and enumeration. Chlortetracycline Selective Supplement (FD120) is an antimicrobic supplement
    containing Chlortetracycline, which inhibits bacteria.
    Due to the selective properties of this medium and the type of specimen being cultured, some strains of fungi may grow poorly
    or fail to grow on the complete medium; similarly, some strains of bacteria may also not inhibited or only partially inhibited.
    Care should be taken not to expose this medium to light, since photodegradation of rose bengal yields compounds that are
    toxic to fungi (13, 15).

    Quality Control
    Appearance

    Please refer disclaimer Overleaf.


    HiMedia Laboratories Technical Data

    Light yellow to pink homogeneous free flowing powder


    Gelling
    Firm, comparable with 1.5% Agar gel
    Colour and Clarity of prepared medium
    Deep pink coloured clear to very slightly opalescent gel forms in Petri plates
    Reaction
    Reaction of 3.16% w/v aqueous solution at 25°C. pH : 7.2±0.2
    pH
    7.00-7.40
    Cultural Response
    M1584: Cultural characteristics observed after an incubation at 25°C for 5 days with added Chlortetracycline Selective
    Supplement(FD120).
    Organism Growth

    *Aspergillus brasiliensis good-luxuriant


    ATCC 16404
    Bacillus subtilis ATCC 6633 inhibited
    Cladosporium good-luxuriant
    cladosporioidesATCC 45534
    Escherichia coli ATCC inhibited
    25922
    Enterococcus faecalis ATCC inhibited
    29212
    Mucor racemosus ATCC good-luxuriant
    42647
    Pencillium notatum ATCC good-luxuriant
    10108
    Saccharomyces cerevisiae good-luxuriant
    ATCC 9763
    *Key: Formerly known as Aspergillus niger ATCC 16404

    Storage and Shelf Life


    Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.

    Reference
    1. Koburger. 1976. In Speck (ed.), Compendium of methods for the microbiological examination of foods. American Public
    Health Association, Washington, D.C.
    2. Mossel, Visser and Mengerink. 1962. Lab Practice 11:109.
    3. Waksman. 1922. J. Bacteriol. 7:339.
    4. Koburger. 1972. J. Milk Food Technol. 35:659.
    5. Martin. 1950. Soil Sci. 69:215.
    6. Tyner. 1944. Soil Sci. 57:271.
    7. Smith and Dawson. 1944. Soil Sci. 58:467.
    8. Cooke. 1954. Antibiot. and Chemother. 4:657.
    9. Jarvis. 1973. J. Appl. Bacteriol. 36:723.
    10. Overcast and Weakley. 1969. J. Milk Technol. 32:442.
    Papavizas and Davey. 1959. Soil Sci. 88:112.
    11. Marshall (ed.). 1993. Standard methods for the examination of dairy products, 16th ed. American Public Health Association,
    Washington, D.C.
    12. Beuchat and Cousin. 2001. In Downes and Ito (ed.), Compendium of methods for the microbiological examination of foods,
    4th ed. American Public Health Association, Washington, D.C.
    13. Clesceri, Greenberg and Eaton (ed.). 1998. Standard methods for the examination of water and wastewater, 20th ed.
    American Public Health Association, Washington, D.C.
    14. Banks, Board and Paton. 1985. Lett. Appl. Microbiol. 1:7.

    Revision : 2 / 2015

    Please refer disclaimer Overleaf.


    HiMedia Laboratories Technical Data

    Disclaimer :
    User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
    this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
    work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
    specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
    for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
    be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

    HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147
    1919 Email: [email protected]

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