Research PPT 101
Research PPT 101
Research PPT 101
(Garcinia mangostana)
PEEL EXTRACT AS AN ALTERNATIVE
TO EOSIN IN WRIGHT’S STAIN
Preparation of smear
Statistical treatment
Preparation of Materials
Utilize in this study will obtain from the local market of City
of Koronadal, South Cotabato, Philippines. While in
obtaining blood samples from 15 students of Notre Dame of
Marbel Univeristy, needle and syringe method of
venipuncture were used. The whole experiment was
conducted inside the Notre Dame of Marbel University
Biology and Chemistry Laboratory wherein the staining and
the extraction process will occur.
Preparation of Mangosteen peel stain
The mangosteen peel will place in a clean plastic container
with sealed lid and stored in room temperature for 24-48
hours before being processed. Extraction and preparation
of the solution were done in line with the study of Chukwu
(2011) and Adegoke et al. (2010) where the mangosteen
peel was washed and dried under the shade for minimum
of 7 days and then milled to form the fine powder.
Preparation of Mangosteen peel stain
The powder will be stored in glass jars and refrigerated at 4°C
until require for use. Fifteen (15), twenty (20), and twenty-five
(25) grams of the powder was weighed using digital balance,
which was dissolved in 100 mL of ethanol in a beaker and then
filtered using the filter paper. It was transferred to 5mL test tubes,
which were centrifuged at 3000 rpm for 5 minutes and
supernatant were collected in a coupling jar with the use of micro
pipettes.
Staining of Blood Smears
The blood collected from the 15 respondents must be
anticoagulated preferably with the disodium or dipotassium
salt of ethylene diamine tetra acetic acid (EDTA).
Staining is done by placing 0.05 mL (50 uL) of blood on a
slide and spread lightly to rapidly produce a smear. The
smea The slide was fixed in the methanol for 5 minutes
and dry. Methylene blue is place upon the smear for 2
minutes and then rinse with running water. Eosin Y is
added on the smear for 1 minute. Then, the slide is rinse
again with water and let to dry (Abnova, 2010).
Staining of Blood Smears
All steps are repeated in another slide except that the
mangosteen peel extract is used in exchange of Eosin Y and
for each blood sample. Four smears were made sorted
according to the following groups:
• Group I (Control Group): Stained with Wright’s stain,
• Group II: Stained with 15 grams in 100 mL ethanol
mangosteen extract,
• Group III: Stained with 20 grams in 100 mL ethanol
mangosteen extract, and
• Group IV: Stained with 25 grams in 100 mL ethanol
mangosteen extract.
Comparison of the staining effects
Description
1 Very Poor no observable morphology of cells
2 Poor stained red blood cell only,
morphological alterations present
3 Satisfactory/Average visible stained red blood cells,
platelets with some morphological
alterations
4 Good visibly stained red blood cell,
platelets with no morphological
alterations
5 Very Good visibly stained red blood cells,
platelets and white blood cells with
no morphological alterations
Insights
The context of this research was to show the staining quality and
capacity of the Mangosteen peel extract as an alternative stain to
Eosin Y in Wright's stain.
As we were writing this research paper, we had the difficulty in
communicating to each other as we are in our new normal
situation. It is quite difficult to share our ideas through video calls
and chats because of the limited features and as well as the
connectivity to the internet.
We also had to revise our title from one to another in order to
come up with a good, easy to understand title.
Insights
In searching for our related literatures, we also had a difficulty as
there were only a few literatures about the mangosteen extract
as an alternative stain in peripheral blood staining. However,
there was a study that concerns about the Mangosteen fruit
extract as an alternative for Histology staining. We incorporated
that study in our research to get recommendations and ideas.
Despite of all these struggles, we managed to overcome it with
the help of the seminars conducted through online meeting. We
also want to thank our advisers for giving us their time and effort
to help us to make our research paper better.
THANK YOU