X Bio Practicals
X Bio Practicals
X Bio Practicals
IAIM
To study the different stages of mitosis using permanent slides.
I ATERIALS REOUIRED
Permanent slides of mitosis, microscope.
PROCEDURE
10X.
Focus permanent slides of mitosis showing different stages under
OESERVATIONS
nuclear division and Cytokinesis or cytoplasmic
Mitosis is divided into two main stages Karyokinesis or
1. Prophase:
The chromosomes begin to coil and become shorter and thicker.
into two followed by duplication of centriole present in it. The daughter centriole
The centrosome splits
moves to opposite poles.
aster rays. Spindle fibres appears between daughter
Each centriole is surrounded by radiating rays called
centrioles.
2. Metaphase:
become thicker and shorter.
The chromosomes further coils and
centromere.
3. Anaphase:
This results in splitting of centromere
The spindle fibres attached to chromosomes starts contracting.
and finally two chromatids of each chromosome separates.
The sister chromatids or daughter chromosomes moves toward opposite poles by the shortening of
spindle fibres.
4. Telophase
Each daughter chromosome lengthens and becomes thinner. The daughter chromosomes are transformed
into thin thread like chromatin.
Class-X II 1
Biology Practical Work-Book I.C.s.E.
Nucleolus reappears in each daughter nucleus.
of the cell. In plant cell. cell
In animal cell. a furrow develops in the cell membrane at the periphery a
CYTOKINESIS
The furrow appeared during telophase stage in animal cell, deepens from periphery to centre and divides
cells.
ac
Chromosome
SOlits inio two
ne
S.ndie Equaiorial Daughter SOl
Fibres Plete m
Disapo2air.g
Early Prophase Late Prophase Metaphase Early Anaphase
agnterhimcsones
ciea
MC ing iarc: DaughiEr
eerne Spnae F:bres
Csapcearing Nucle Daugnter
Cells
MATERIALS REQUIRED
Thistle hunnel, cellophane beaker, 20%
paper, sucrose solution, water, iron stand.
PROCEDURE
Cover the mouth of the thistle funnel with
cellophane paper and tie it securely. Invert the thistle Levei of Solution.
funnel inbeaker containing water and fix it to the iron Aiter Sometime
stand in the shown
position. Add sucrose solution to
the thistle funnel. Mark the initial level of the
sugar 20
solution on the stem of the thistle funnel. Initial Lel
Keep this Sucrosel Level
experimental set up for 4 - 5 hours.
ucrose
IN OBSERVAT!ON Beaker.
Water
The level of sucrose solution rises in the thistle Thistie
funnel. The level of water in the beaker drops. Funnei
Semipeimeabe
Memorane
PRECAUTIONS
The sucrose solution should be poured carefuly in order to avoid air bubble in the
thistle funnel.
The initial level of the sucrose solution should be marked
only after immersing the mouth of the thistle
in beaker containing water.
EXPERIMENT - 3
I AMA
To study the process of
transpiration using bell jar.
I MATERIALS REQUIRED
Bell jar, potted plant, polythene bag, glass plate, grease
I PROCEDURE
Take a wel-watered plant. Cover the
pot up to the stem with a polythene bag and secure
thread. Place the pot on a
glass plate and cover it with a bell jar. Grease the edges of the bell tightly
w
Poted Plant
Covered Pot
Glass Sheet
Leaves of the potted plant looses water in the form of water vapour. The vapours condense on the inne
surface of the bell jar
IPRECAUTIONS
1. Grease the edges of the bell jar to avoid entry of moisture from outside.
2 Cover the pot with polythene and tie tightly to avoid evaporation of water from the soil.
EXPERIMENT - 4
IAtM
o measure the of
rate water uptake by the leafy shoot using Ganong s potometer.
MATERIALS REQUIRED
Leaty shoot. beaker, water. split cork, vaseline. Ganong s potometer. coloured water.
GAOKS
GANONG 'S FOTOMETE
POTOMETER
It consist of a horizontal graduated capillary tube. One end of capillary tube opens into a wide tube that
bears two vertical tubes. First vertical tube is called water reservoir. t has a stopcock at its base to release water
from it.
The second vertical tube is meant for holding a leafy shoot. The other end of capillary tube is bent into
a tube .The entire apparatus is mournted on a stand.
IPROCEDURE
Fill Ganong's potometer with water to make it free from air spaces. Apply vaseline to the upper opening of
the vertical tube meant for holding leafy shoot. Take a fresh leafy shoot of a herbaceous plant cut under water.
Fix it in the vertical tube through a split cork. Dip the bent capillary tube in a beaker containing coloured water.
Introduce an air bubble in the capillary tube. This can be done by lifting the bent tube above the coloured water
so that air is sucked in and dipping it again into the water. Note down the initial position of the air bubble in the
capillary tube. Place the apparatus in the sunlight.
OBSERVATIONS
The air bubble in the capillary tube moves towards the shoot.
Leafy Shoo
Reservoi
Cork
4 5 6 7 8 9 10 11 12 13
2
23::2::3::::22::::3:E333
Graduated
Capillary tube
Stand
Beaker
IPRECAUTIONS
The entire apparatus should be airtight.
2 The leafy shoot should be cut obliquely to increase surface for water absorption.
3 The leafy shoot should be cut under water to prevent air bubble entering into the shoot, which will
block the passage of water.
EXPERIMENT - 5
IIPROCEDURE
short-stemmed funnel
Place Hydrilla plant in a beaker containing water. Cover Hydrilla plant by inverting a
Over it. Invert a test tube full of water over the stem of the funnel. Place the set up in the sunlight.
OBSERVATION
C er
Bubbles of gas rises in the test tube and
collects upward. Water is displaced downward. BuDbieS
Sun iigh
Allow the test tube to be filled with the gas.
Remove the test tube and introduce glowing
splinter. It burst into flames in the presence of Shor
gas. This confirms the presence of oxygen. Steme
Funne Beake
INFERENCE
Hyarilie
Funne Raised
Hydrilla is aquatic plant, which carries
an
from Bottom
out photosynthesis. During photosynthesis,
Oxygen is evolved as a bye product.
Fig.: Experiment to prove that oxygen is evolved during photosynthesis
IPRECAUTIONS
1 The stem of the funnel should be below water in the beaker.
2 The apparatus should not be disturbed once the process starts.
3 Cut ends of the Hydrilla should be facing towards the stem of the funnel.
EXPERIMENT - 6
AlM
To study the structure of human heart.
OBSERVATIONS
Its size is
Human heart is a muscular cone located in thoracic cavity between two lungs.
shaped structure
left.
heart is downwards, pointing towards
about the size of a clenched fist of the same person. The apex of
The layers of pericardium enclose
Heart is covered by a double walled membrane called pericardium.
heart from
of heart during heart beat and protects
pericardial fluid. This fluid allows frictionless movement
mechanical injuries.
Aorta
(Main Artery)
Pulmonary Artery
- Aorta (To Right Lung)
Superior Pulmonary Artery
Vena Cava l o Let Lung
PTUPARTE
ulmonary Superior Vena Left Atrium
Veins Cava (Main Vein Pulmonary Veins
Let Atrium from Upper Body) TFrom Right å Left
Semi Lunar Lungs
RIgh Valves Bicusid Value
Airium
Let ventricie Right Atrium
nter-ventricular
Coronany Tricuspid
va us SEprum
Biood Vessals
eit venicie
InienorVena
Inienor 2ra
Cave a a a n Ven Cardaus
:cht niric rom- r oc 3.gnentricle
heart
2 Internal Structure of the
- External Structure of the heart
atrio-ventricular or tricuspid
ventricle is guarded by right
The aperture between right auricle and right thread like structure
to the papillary muscles through
valve. It consists of three flaps, which are attached
Class-X
IIBiology Practical Work-Book I.C.S.E.
16
of blood trom
called chordae teiidinae. It holds valve in its place. Tricuspid valve prevents the back flow
PROCEDURE
ake two glass slides and clean with 90% alcohol. Select middle or ring finger and clean the area to be
punctured with alcohol. Puncture the tip of the finger with the disposable needle. Wipe away the first drop of
blood with clean gauze. Touch the next drop of blood with a clean slide. Bring a clean spreader slide, held at a
45 angle, toward the drop of blood on the specimen slide. While holding the spreader slide at the same angle,
push it forward rapidly and smoothly. Wait until the thin film of blood completely dry. Fix the thin film with
methanol and let it dry. Put few drops of stain on the slide until it is fully covered. Then rinse the slide with
cistilled water at room temperature. Drain off the water and leave the slide to dry. A permanent slide of blood
can also be used to focus different types of blood cells.
I OBSERVATIONS
Blood is a fluid connective tissue. t consist of plasma and blood clls. The following blood cells can be
observed in the slide:
) Erythrocytes or Red bleod celis (RBC) These are minute biconcave disc like structures. The mature
red blood cells without nucleus. Thus it becomes flat in the center and
are concave on both the sides
RBCs contain respiratory pigmernt called haemoglobin.
1 2 3
18
HIBiology Practical Work-Book I.C.S.E. Class-X
hese
WBCs are amoeboid and can produce pseudopodia.
aioebid
celis nave distinct nucleus. The can squeeze through capillary wali into the tissues b
movement. These do not contain haemoglobin. WBCs are broadly classitied as tollowing
( Thrombocytes or Platelets These are minute, oval or round structure without nucleus.
Granules
Granulocyte Nucleus
S-shaped
Nucleus
Erythrocyte
Eosinophil
Nucleus
Basophil
Monocyte
Agranulocyte
Polymorphic Lymphocyte
Nucleus
Neutrophil
I MATERIALS REQUIRED
Model and chart of urinary system in man.
I OBSERVATIONS
Human urinary system consist of the following parts
) Kidney : A pair of bean shaped kidneys are present in the abdominal cavity on either side of the vertebral
column. Last two pair of ribs protect kidneys. Kidneys are attached to body wall. Each kidney is about
10 cm long, 5 cm in breadth and 4 cm in thickness. Each kidney has a convex (outer) and a concave
(inner) side. On concave side, a fissure called hilum is present
The right kidney is slightly lower than the left kidney. Each kidney is made of one million of nephrons
or uriniferous tubules. Nephrons are structural and functional unit of kidney.
Adrenal gland is present on each kidney.
(i) Ureter I t is tube of nearly 30 cm, which arises from funnel shaped structure called pelvis of each
kidney. It carries urine from kidney to the urinary bladder.
MATERIALS RECJIRED
Model and chart of human brain.
OBSERVATIONS meninx)
membranes called meninges (singular:
Human brain is protected by
(brain box). A protective
craniumn
consist of following
three layers
covers the brain. Meninges
the other.
and inner cerebral medulla. Cerebral
differentiated into outer cerebral cortex
The wall of cerebrum is Cerebral
in colour, it constitutes gray matter.
cortex is made of cell
bodies of neurons. Since it is gray
neurons. Being white in colour,
it constitutes white matter
medulla is made of axons of myelinated
activities.
and reasoning. It controls all voluntary
Cerebrum is the seat of intelligence, memory
under the cerebrum. Its outer layer is
made up of gray matter
brain) : It is located
() Cerebellum (little tree. Cerebellum
matter. White matter appears like a branching
while inner layer is made up of white
muscular activities and maintains
convolutions. Cerebellum coordinates
has numerous furrows but no
body equilibrium.
hemisphere of cerebellum to other.
It carries impulses from one
(ii) Pons It is located below cerebellum.
which continues below as spinal cord. White
(iv) Medulla Oblongata It is the lowermost part of the brain,
matter. Medulla oblongata controls
matter forms the outer layer while inner layer is made of gray
movement of alimentary cana
involuntary actions of the body like breathing, heartbeat, peristaltic
coughing, sneezing, constriction and dilation of blood vessels etc.
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i.c.s.E. lass-X
Biology Practical Work-Book
Choroid pleus
of third, entriCIe
OSum
Aqueduct of sylvius
Pareta obe
Pineal body Thalamus
Hyootaamu
Corpora Opuc riasma
OcCipital
quadr:gamina(
intunaulan
b e Fourth ventrice Pituitary body
I
ATERALS REQUIRED
MATERIALS FREQUIRE
Model and chart of human eye.
OBSERVATIONS of eyeball
eye sockets.
The movement
Human eyes are located in deep cavities of skull called orbits or
tears and washes away dust particles from the surface of eye. Tears
have lysozymes, which kilis the
germ.
Choroid t is highly vascular and pigmented layer. The dark colour of the eye prevents internal reflection
of the light inside the eye. The choroid layer expands in front to form ciliary body. The cihary body
possesses ciliary muscles, which are attached to lens through suspensory ligaments. Ciliary muscles
helps to alter the shape of the lens. A pigmented muscular layer develops from choroid in front of the
ciliary body. This is called iris which has a central aperture called pupil.
Iris controls the size of pupil. Dilation and constriction of pupil control the amount of light entering the
eye.
Retina The innermost photosensitive layer of eyeball is called retina. It contains photoreceptor cells
called rods and cones .The layer of nerve cells present in retinal layer give rise to optic nerve. The exit
of optic nerve in the retinal layer is called blind spot. In the optical axis on retina. there is a slight
depression called yellow spot or fovea centralis. It has maximum cone cells and represents the site of
brightest image. Rod synthesizes pigment rhodopsin or visual purple which helps in vision in dim light.
Cone cells synthesize pigment iodopsin which helps in vision in bright light.
Biology Practical Work-Book 1.C.S.E. Class-x 23
Lens
ens lt is a transparent crystalline and biconvex structure located behind the pupil.
filled with
Aqueous chamber and the lens is called aqueous chamber. It is
I he space between cornea
lens moist and refracts the light entering
watery iquid called aqueous humour. Aqueous humour keeps the
the eye
Vitreous chamber I t is the behind the lens that is filled with thick jelly like fluid called
large cavity
vitreous humour. Vitreous humour helps to maintain the shape of the eye ball.
Cillary Muscle
Eyelid Sclera
Choroid
Tear Gland- Retina
Eyelash
Conjunctiva- Fovea or Area
Cornea
Cenralis
Pupil
I MATERIALS REQUIRED
I OBSERVATIONS ear
(1) External
consist of three parts :
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1.c.s.E. Class-Xl
Blology Practical Work-Book
".
to
Auditcry nerve The nerve fibre from semicircular canal, cochlea. utriculus and sacculus joins ror
auditory nerve. It carries nerve impulse trom ear to brain.
Semicircular
Inferior
/Horizontal
Semicircular
cannais
Incus Stapes, Canals Superior
Malleus
Auditory
Nerve
Utriculus
Nerve
J U L
Saculus
Cochlea
Tympanum Cochlear Duct
Pinna
Eustachian
Tube
Il. Thyroid
endocrine gland, weighing around 20 g in an adult.
The thyroid is the largest
is located just interior to the
larynx.
The butterfly-shaped thyroid
3 The right and left lateral lobes lie one on either side of the trachea
4 Structurally, the thyroid gland consists of about three million spherical or oval sac-like
follicles.
5. Thethyroid secretestwoimportant hormones : (1) thyroxine, and (i1) triiodothyronine.
Pituitary
Thyroid
Adrenal/suprarenal
gland
Pancreas
In female only
Ovary
In male only
Testis
Fig. 1. Main endocrine glands of humans.
48 NOOTAN ICSE Bioiogy M
ll. Pancreas
1. Pancreas is gland that is partly exocrine and partly endocrine.
a
Insulin and glucagon are the two important hormones of the islets of Langerhans.