Complete Syllabus of MSC MB
Complete Syllabus of MSC MB
Complete Syllabus of MSC MB
FACULTY MEMBERS
Sl.No. 1.
Name Dr.Kiran.B
2. 3. 4.
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SCHEDULE
ODD SEMESTER 2 week of September to 4th week of December 16 6 6 8.30 a.m. to 3.00 p.m.
nd
EVEN SEMESTER 3rd week of July to 2nd week of December 16 6 6 8.30 a.m. to 3.00 p.m.
Duration No. of Working Weeks No of days per week No of hours per day Timings
EVALUATION For the course, there will be both Continuous Internal Assessment (CIA) and End Semester Examination (ESE) with weightage in the ratio of 40:60. I CONTINUOUS INTERNAL ASSESSMENT (CIA): EVALUATION PATTERN 1. THEORY PAPERS: Maximum marks 100 (40 marks for Internal assessment and 60 marks for End Semester Examinations) Break up for internal assessment: Test 1 - 10 marks Test 2 - 10 marks Assignments - 10 marks Other academic activities proposed by the Department and approved by the Principal 10 marks 2. PRACTICALS: Maximum marks 100 (40 marks for internal assessment and 60 marks for End Semester Examinations)
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(other than the original evaluators).There will be no revaluation for practical examinations. e. PASS REQUIREMENT: A minimum of 50% in each subject (theory and practicals) separately both in CIA and ESE is mandatory. Note: Exemption, may be given for participation in co-curricular, extracurricular, sports and other activities subject to the fulfilment of other required conditions. f. DECLARATION OF CLASS Class will be awarded taking the aggregate of all the subjects Distinction 75% and above First Class 60% to 74.9% Second Class 50% to 59.9 g. RANKS Top 10 % ranks will be declared on the basis of aggregate marks of all the semesters / years put together for each course Students are not eligible for ranks :When they have not cleared any paper in the first attempt, When they have been booked for malpractice h. ISSUE OF MARKS CARDS After the declaration of semester results, students are issued a marks statement by the institution. i. AWARD OF DEGREE CERTIFICATE On successful completion of the course, the degree certificate is awarded by the Bangalore University.
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DISTINCTIVE FEATURES OF THE DEPARTMENT Microbiology is the study of living organisms of microscopic size, which includes Bacteria, Fungi, Algae, Protozoa and the infectious agents at the boarder line of life that are called viruses. It is concerned with their forms, structure, reproduction, physiology, metabolism and their classification. It includes the study of their distribution in nature and their relationship to each other and to other living organisms, their effects on human beings and on other animals and plants, their abilities to make physical and chemical changes in our environment and their reaction to physical and chemical agents. If you were to dust your desk and shake your dust cloth over the surface of a medium designed for growing microorganisms, after a day or so you would find a variety of organisms growing on the medium. If you were to cough onto such a medium or make fingerprints on it, you would later find a different assortment of microorganisms growing on the medium. Thus microorganisms have a close association with humans. As we enter the next century, microbiology continues to play an important role in our world. There is a wide scope in the field of medical microbiology, new and emerging infections such as the Ebola virus and drug resistant bacteria continue to plague and perplex scientists. Although smallpox and a few other diseases have been effectively eliminated, there has been a resurgence of tuberculosis, polio, and other diseases that once were thought conquered. Infectious diseases remain the leading cause of death in the world. Diseases such as Acquired Immune Deficiency Syndrome (AIDS), caused by the human immunodeficiency virus (HIV), destroy our bodys immune system and affect million of people worldwide.
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Course Structure CMR Institute of Management Studies (Autonomous), Bangalore I semester M.Sc., Microbiology Course structure PAPER CODE 1 MMB 01 1 MMB 02 1 MMB 03 1 MMB 04 TITLE OF THE PAPER Microbial Diversity Bacteriology and Virology Phycology & Mycology Microbiological Techniques
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CMR Institute of Management Studies (Autonomous), Bangalore II semester M.Sc., Microbiology Course structure PAPER CODE 2 MMB 01 2 MMB 02 2 MMB 03 2 MMB 04 2MMB 05P 2 MMB 06P 1 MMB PR1 Microbial Genetics Microbial physiology Immunology Biostatistics, bioinformatics, Research methodology and Bioethics Microbial physiology and Microbial genetics Immunology and Bioinformatics Project-1 TITLE OF THE PAPER
CMR Institute of Management Studies (Autonomous), Bangalore II semester M.Sc., Microbiology Value Added Course Course structure PAPER CODE 1 MMB07V 1 MMB07VP TITLE OF THE PAPER Food Science and Nutrition Food Science and Nutrition
CMR Institute of Management Studies (Autonomous), Bangalore III semester M.Sc., Microbiology Course structure
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CMR Institute of Management Studies (Autonomous), Bangalore IV semester M.Sc., Microbiology Course structure PAPER CODE 4 MMB 01 4 MMB 02 4MMB 03 4 MMB 04 4MMB 05P 4 MMB 06P 1 MMB PR 1 TITLE OF THE PAPER Agricultural Microbiology Medical Microbiology Industrial Microbiology Fermentation technology Agricultural and Medical Microbiology Microbial and Fermentation Technology Project I
CMR Institute of Management Studies (Autonomous), Bangalore IV semester M.Sc., Microbiology Value Added Course Course structure PAPER CODE 2 MMB07V 2 MMB07VP TITLE OF THE PAPER Molecular Diagnostics Molecular Diagnostics
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1 MMB 03
52
60
40
100
1 MMB 04
120
60
40
100
1 MMB 05P
120
60
40
100
1 MMB 06P
1 MMB PR1
6 38
CMR Institute of Management Studies (Autonomous), Bangalore II semester M.Sc., Microbiology Marks Distribution PAPER CODE TITLE OF THE PAPER NO. OF HOURS Per week 2 MMB 01 2 MMB 02 2 MMB 03 2 MMB 04 Microbial Genetics Total ESE MARKS CIA TOTAL
52
60
40
100
Microbial physiology
52
60
40
100
52
60
40
100
52
60
40
100
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CMR Institute of Management Studies (Autonomous), Bangalore II semester M.Sc., Microbiology Value Added Course Marks Distribution TITLE OF THE PAPER NO. OF HOURS Per week Food Science and Nutrition Food Science and Nutrition TOTAL 2 3 5 Total 40 30 70 ESE 60 60 120 MARKS
PAPER CODE
CIA 40 40 80
1 MMB07 V 1 MMB07VP
CMR Institute of Management Studies (Autonomous), Bangalore III semester M.Sc., Microbiology Marks Distribution PAPER CODE TITLE OF THE PAPER NO. OF HOURS Per Total week 4 52 4 4 4 8 52 52 52 120 MARKS ESE 60 60 60 60 60 CIA 40 40 40 40 40 TOTAL 100 100 100 100 100
Molecular biology Recombinant DNA Technology Environmental Microbiology Food Microbiology Molecular Biology and Recombinant DNA Technology Environmental and Food Microbiology Project I
8 6
120 90
60
40
100
NA
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CMR Institute of Management Studies (Autonomous), Bangalore IV semester M.Sc., Microbiology Marks Distribution PAPER CODE TITLE OF THE PAPER NO. OF HOURS Per week 4 4 4 4 8 Total 52 52 52 52 120 60 8 120 60 6 38 90 538 60 420 40 40 280 100 100 700 40 100 ESE 60 60 60 60 MARKS CIA 40 40 40 40 TOTAL 100 100 100 100
4 MMB 06P
1 MMB PR 1
Agricultural Microbiology Medical Microbiology Industrial Microbiology Fermentation technology Agricultural and Medical Microbiology Microbial and Fermentation Technology Project I TOTAL
CMR Institute of Management Studies (Autonomous), Bangalore IV semester M.Sc., Microbiology Value Added Course Marks Distribution TITLE OF THE PAPER NO. OF HOURS Per week Total ESE MARKS
PAPER CODE
CIA
TOTAL
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CMR Institute of Management Studies (Autonomous), Bangalore PG Department of Microbiology QUESTION PAPER PATTERN Total Questions given 12 Questions (2 Choice) 9 Questions (2 Choice) 4 Questions (1 choice)
SECTION
TEST Answers in brief Answers in brief and concepts Answers in detail with scientific knowledge
QUESTIONS
MARKS
TOTAL
10
2 Each
20
5 Each
35
15 Each
45
Total
100
CMR Institute of Management Studies (Autonomous), Bangalore PG Department of Microbiology QUESTION PAPER PATTERN Value added course
SECTION
QUESTIONS
MARKS
TOTAL
2 Each
10
5 Each
20
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15 Each
30
3 Questions (1 choice)
Total
60
Pattern of marks distribution for internal marks: CONTINUOUS INTERNAL ASSESSMENT (CIA) Theory Attendance 5 Practicals Attendance 5 Record 5 Performance 20 Viva voce 10 Total 40 Seminars 5 Assignments 10 I Internals 10 II internals 10 Total 40
Project Viva voce Introducti on & collection of review of literature 4 Problem & hypothesis 4 Sampling and Tools for data collection 4 Material and Methods/stasis tical analysis 4 Result, Discussio n and conclusio n 4
Attendan ce
Total
10
10
40
END SEMESTER EXAMINATIONS (ESE) Practicals Experiments/ Spotters 40 Viva voce 10 Record 10 Total 60
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Viva voce
Result
30
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References: 1. Johri,B.N. Extremophiles, Springer Verlag, New York, 2002. 2. Allsopp,D.,Colwell, R.R., and Hawksworth,D.L.(1995) Microbial Diversity and Ecosystem Function, 2004. 3. Black, Microbiology-Principles and Explorations, 6th edition, 2005. 4. Daniel Lim, Microbiology, 2nd edition, 2006 5. Jeffrey C. Pommervile, Alcamos Fundamentals of Microbiology, 8th edition, 2006. 6. Maier, Pepper, Environmental Microbiology, 2nd edition, 2004 7. M.T Madgan and others, Brock Biology of Microorganisms, 9th edition, 2002 8. L.M Prescott and others, Microbiology, 4th edition, 2003 9. Stanier, General Microbiology, 5th edition, 2007 10. Hurst, Crawford, Manual of Environmental Microbiology, 2nd edition, 2001 11. Nester E.W. and others, Microbiology, 3rd edition, 2003 12. Pelczar, Microbiology, 5th edition, 2006 13. David C.Sigee, Freshwater Microbiology, 2nd edition, 2002 14. Gerard J. Tortora, Microbiology- An Introduction, 8th edition, 2004
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CMR INSTITUTE OF MANAGEMENT STUDIES (AUTONOMOUS), BANGALORE I SEMESTER M.SC., MICROBIOLOGY 1 MMB 02: BACTERIOLOGY AND VIROLOGY
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10
10
14
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CMR INSTITUTE OF MANAGEMENT STUDIES (AUTONOMOUS), BANGALORE I SEMESTER M.SC., MICROBIOLOGY 1 MMB 03: PHYCOLOGY AND MYCOLOGY 52 HOURS CONTENT Morphology And Classification Of Algae : Media and methods used for culturing algae, measurement of algal growth, strain selection and large scale cultivation; applications therapeutic uses, heavy metal removal, use of immobilized and labelled algae, economic importance of algae. Mycology : Historical introduction to Mycology; Evolutionary tendencies in lower fungi- salient features and examples of Divisions Myxomycota - Acrasiomycetes, Hydromyxomycets, Myxomycetes, Plasmodiophoromycetes; Zoosporic fungi Chytridiomycetes, Hyphochytridiomycetes, Oomycetes, Zygomycotina - Zygomycetes, Trichomycetes Study Of Higher Fungi : Study of higher fungi - salient features and examples of Divisions Ascomycotina; Basidiomycotina ; Blastomycetes Structure And Growth Of Fungal Cells : Hyphae and non-motile unicells, motile cells, spores, dormancy, growth of population and colonies, mechanics of growth in fungi, Measurement and Kinetics of growth, nutritional and environmental requirements; effect of environment on growth - pH, temperature; prevention of fungal growth. Fungal Ecosystem : Heterothallism, Sex hormones in fungi, Physiological specialization, Phylogeny of fungi; Substrate groups - saprophytic, parasitic, Keratinophilic, coprophilous; NO. OF HRS 10
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Fungal Diseases : Fungal diseases - mycoses, systemic and subcutaneous, candidiasis, pnemocystis, blastomycoses, dermatomycoses Reference: 1. Alexopoulos, C.J., and Mins C.W (2001) Introduction to Mycology, Wiley 2. Becker, E. W., (1994) Microalgae: Biotechnology and Microbiology, Cambridge University Press. 3. Charlie M., and Walkinson, The Fungi. Academic Press
CMR INSTITUTE OF MANAGEMENT STUDIES (AUTONOMOUS), BANGALORE I SEMESTER M.SC., MICROBIOLOGY 1 MMB 04: MICROBIOLOGICAL TECHNIQUES 52 HOURS CONTENT Microscopy : Working principle, different types- Light microscopy, Bright field, Dark field, Phase contraction microscopy, Fluorescent microscopy, Electron microscopy (TEM and SEM), Confocal Microscopy, Polarisation microscopy Scanning probe microscopy, Haemocytometry and Microphotography. X-ray diffraction, Crystallisation. Sterilization Techniques : Definitions of Physical methods- Heat (dry & moist) Filtration-Types of Filters, Radiation-Ionising and Non-ionising; Chemical agentsPhenols, Alcohols, Halogens, Heavy metals,Aldehydes,Quaternaryammonium ,compounds and Gases. Evaluation of antimicrobial agent- Phenol coefficient, Filter paper method. Isolation Of Pure Cultures : Pure Culture Techniques- Dilution, Spread plate, Streak plate, Pour plate, Micromanipulator Method. Maintenance and Preservation of Pure NO. OF HRS
10
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Measurement Of Microbial Growth& Staining Technique : Direct method- Direct Microscopic count, Standard plate count, Filtration, MPN, Indirect methodDetermination of Turbidity, Metabolic activity, Dry weight and Micrometry.Nature of stains, Principle, Mechanism, Method and Types of Staining-Simple, Differential and Special staining. Instrumentation : Spectroscopy: Principle, Construction and Application of Spectrophotometer, Different types, Working, Principle & their applications. Chromatography, Electrophoresis, and centrifugation- Different types and Applications. Radioisotopic Techniques : Stable and Radioactive isotopes, Radio isotopic Autoradiography, Scintillation counters, Non-radioactive labelling. Reference: 1. James, G.N. Microbiology A laboratory manual, Addison- Wesley. 2. Norris, J.R. and Ribbons, E.W. Methods in Microgiology, Academic press 3. Bhattacharlya. Experiments with microorganisms, Emkay publicationjs 4. Plummer: An Introduction to Practical Biochemistry, Tata-McGraw Hill
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CMR INSTITUTE OF MANAGEMENT STUDIES (AUTONOMOUS), BANGALORE I SEMESTER M.SC., MICROBIOLOGY 1 MMB 06P: PHYCOLOGY, MYCOLOGY AND MICROBIOLOGICAL TECHNIQUES
NO 1
TITLE OF THE EXPERIMENTS Isolation of fungi from different substrates (Saprophytic, Parasitic, Copraphilous, Keratinophilous)
Study of environmental requirements of Fungi (pH, Temperature) by linear growth and biomass.
3 4 5
Study of antifungals (antibiotics/ Chemicals/ Plant extracts) Study of antagonism Isolation of pathogenic fungi from infected samples; human/plant/animals
6 7 8 9
Germ tube test Viability test Isolation of epiphytic algae. Isolation and identification of algae from various aquatic environments. (polluted / fresh water)
10 11
Lichens; Morphological study Isolation and characterization of acidophilic and halophilic fungi.
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SYLLABUS II SEMESTER
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CMR INSTITUTE OF MANAGEMENT STUDIES (AUTONOMOUS), BANGALORE II SEMESTER M.SC., MICROBIOLOGY 2 MMB 01: MICROBIAL GENETICS 52 HOURS CONTENT Structural Polymorphism Of DNA : DNA structure: A, B and Z DNA, Supercoiled DNA, DNAh and binding proteins, DNA replication in Prokaryotes (double stranded DNA virus, Pox Virus; SV4O; ssDNA virus. The rolling circle replication, semi conservative replication, primer or template, origin of replications, replication fork- leading and lagging strands, enzymes involved at different steps of replication, Folded fiber model of E.coli chromosome, Genome organization in eukaryotes, Split genes, overlapping genes, DNA amplification . The law of DNA constancy and- C-value paradox. Replication Of RNA : Replication of plus strand RNA virus (polio), Negative strand RNA virus, r (VSV and influenza). Replication of double stranded RNA virus (Rotavirus), antisense RNA Mutation : Gene as unit of mutation, Molecular basis of spontaneous and induced mutations and their role in evolution; Mutagens, Types of mutations, transposon mutagenesis site directed mutagenesis, AMESs test; environmental mutagenesis and toxicity testing. Genetic Recombination At Molecular Level : Genetic recombination in bacteriophages and E. coil, Synapsis of homologous duplexes, breakages and re-union, role of Rec A in recombination, generalized & specialized transduction, transformation and conjugation, Legitimate & illegitimated recombination gene conversion, overview of bacterial genetic map Gene Transfer Mechanisms : Bacterial transformation; Host cell restriction; Transduction; Complementation; conjugation and transfection, Mechanisms and applications, Genetic analysis of microbes, Bacteria and yeast. Genetics of fungi-alteration of generation, neurospora crassa and yeasts induction of mutation, cytoplasmic inheritance and biochemical mutants Plasmids : Plasmids, F-factors - description and their uses in genetic analysis. Colicins and Col Factors R plasmids, Plasmids as vectors for gene cloning, Replication of selected plasmids: compatibility, Transposns and their use in genetic analyses. Bacteriophages: Lysogeny and lytic cycle in bacteriophages, Life cycle and their uses in microbial genetics. Lytic phages-T7 and T4, Lysogenic phages Lambda and P1. Ml 3 and X 174. Reference: 1. Dale, J.W. Molecular Genetic of Bacteria 2. Benjamin Lewin, Genes VIII 3. Streips and Yasbin. Modern Microbial Genetics 4. John Ringo, Fundamental Genetics NO. OF HRS
10
7 10
10
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CMR INSTITUTE OF MANAGEMENT STUDIES (AUTONOMOUS), BANGALORE II SEMESTER M.SC., MICROBIOLOGY 2 MMB 02: MICROBIAL PHYSIOLOGY 52 HOURS CONTENT Microbial Nutrition : Organic and inorganic nutrients: Basic aspects of energetics-entropy, enthalpy, Electron transport chain, artificial electron donors-uncouplers-High energy bond phosphorylation. Brief Account Of Photosynthetic And Accessory Pigments : Brief account of photosynthetic and accessory pigments-chlorophyll-bacterial chlorophyll- rhodopsincarotenoids-phycobiliproteins; Carbohydrates anabolism autotrophy oxygenicanoxygenic photosynthesis; autotrophic generation of ATP; fixation of CO2-Calvin cycle-C3 and C4 pathways; Chemolithotrophy ,luminescence; Biosynthesis of fatty acids; pigments and secondary metabolites. Coordinated control of metabolism. Enzymes : Definition, structure, Enzymes as biocatalysts, properties and classification, specificity, active sites, Coenzyrnes: Activators and inhibitors, activity unit, Isozymes, Enzyme kinetics (negative and positive cooperatively); Michaelis-Menten equation for simple enzymes. Determination of kinetic parameters, multistep reactions and rare limiting steps, enzyme inhibition, allosterism. kinetic analysis of allosteric enzymes, principles of allosteric regulation, Ribozyme and abzyme. Respiratory Metabolism : Emhden Meyerhoff Parns pathway: Entner Doudoroff pathway; glyoxalate pathway; Krebs cycle; Oxidative and substrate level phosphorylation, reverse TCA cycle; glucon eogenesis, Pasteur effect; fermentation of carbohydrates-homo and hetero lactic fermentation.HMP Assimilation Of Nitrogen : dinitrogen, nitrate nitrogen, ammonia, synthesis of major amino acids, polyamines; synthesis of polysaccharides-peptidoglycan-biopolymers as cell components; endospores-structure, properties and germination. Biosynthesis of purines and pyrimidines Signal Transduction In Prokaryotes And Eukaryotes : Overview of signal transduction, signals, second messengers, termination of signal transduction. Prokaryotic signal transduction:Bacterial pheromones, chemotaxis, flagella movements.phototaxis. signaling mechanisms involved in eukaryotic cell division, glucose metabolism Reference: 1. Lehninger, Principles of Biochemistry 3dr edition by Nelson & Cox (Worth) 2000 pub. 2. Moat. A.G. & Foster J.W. 1999 Microbial Physiology, Wiley-Liss. 3. Arora D.K. & Seema Gupta.1996. Bacterial Physiology Anmol Publications. New Delhi. NO. OF HRS
13
10
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CMR INSTITUTE OF MANAGEMENT STUDIES (AUTONOMOUS), BANGALORE II SEMESTER M.SC., MICROBIOLOGY 2 MMB 03: IMMUNOLOGY 52 HOURS CONTENT Immune System And Immunity : History of immunology; Structures, composition and functions of cells and organs involved in immune system - T- cells, B-cells, macrophages, antigen-processing cells, Eosinophls, Neutrophils, Mast cells and killer T - cells; microbial infections and immune reponses - innate Immunity, acquired Immunity; Clonal nature of immune response; Immunohaematology- blood groups, blood transfusion and Rh incompatibilities. Antigens And Antibodies : Antigens - structure and properties, types - Iso and all antigens - haptens; adjuvants - antigen specificity. Immunoglobulins - Structure heterogenecity - types and subtypes - properties (physico-chemical and biological); pathways. Generation of immunological diversity; Effector mechanisms., T- cell cloning and its applications in vaccine development, production of monoclonal and polyclonal antibodies and its applications In vitro Methods -Agglutination, precipitation, complement fixation, immunofluorescence, immuncelectrophoresis, ELISA, Radio Immunoassays; In vivo Methods: skin tests and immune complex tissue demonstrations. Applications of these methods in diagnosis of microbial infections, auto immunity mechanisms, systemic lupus erythematosus, Graves diseases, rheumatoid arthritis, myasthenia gravis, multiple sclerosis. Immunodeficiency- phagocytic humoral, CMI, combined HLA association Major Histocompatibility Complex And Tumor Immunology : Structure and functions of MHC and HL-A systems. Gene regulation and immune response genes HLA and tissue transplantation - Tissue typing methods for organ and tissue transplantations in humans; graft versus host reaction and rejection; autoimmunity Tumor immunity - tumor specific antigens, Immune response to tumors, immunodiagnosis of tumors - detection of tumor markers - alphafoetal proteins, carcinoembryonic antigen etc., 18 NO. OF HRS
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13
13
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10 11 12 13 14
Sequence analysis/similarity search: blast, fasta, clustal Structure visualization: rasmol Structure prediction: secondary and tertiary Restriction mapping Primer designing
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CMR INSTITUTE OF MANAGEMENT STUDIES (AUTONOMOUS), BANGALORE II SEMESTER M.SC., MICROBIOLOGY 1 MMBV: Food Science and Nutrition VALUE ADDED COURSE 40 HOURS CONTENT NO. OF HRS Introduction : Introducing the subject and defining the syllabus of the course Human Nutrition : FOOD NUTRIENTS: Study of seven food componentsCarbohydrates, Proteins, fats, Vitamins, Minerals and Fiber. Their chemical structure, Classification, Functions, Sources and Deficiencies, Water balance. ENERGY VALUE OF FOODS AND ENERGY REQUIREMENT: Determination of energy value of foods,defining basal metabolism,nutritive value of foods, food groups, recommended dietary allowance Functional Properties : EFFECT OF PROCESSING ON NUTRITIVE VALUE OF FOODS: Effect of processing like cooking, soaking, steaming, germination, frying etc., on the food nutrients and the overall nutritive value. A. SENSORY EVALUATION: Sensory attributes, grades, ranking and factors affecting the sensory evaluation pattern. B. PHYSICO CHEMICAL PROPERTIES: Study of emulsions, gels, colloids, foams etc., Functional properties of proteins and starch colloids. Browning reaction in foods. C. FATS AND OILS: Properties, manufacturing process, uses in food systems, rancidity, smoking point. D. FERMENTATION TECHNOLOGY: Process of fermentation and the physico chemical changes in food nutrients during fermentation. Significance of fermentation. E. ROLE OF ENZYMES IN FOOD PROCESSING Product Development And Marketing : Changing Trends Of Food Production, Food fortification, packaging, food additives and food flavours. Nutritional labelling. Entrepreneurship skills and marketing. Clinical Nutrition : Brief insight into Therapeutic Diets 1
10
19
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CMR INSTITUTE OF MANAGEMENT STUDIES (AUTONOMOUS), BANGALORE II SEMESTER M.SC., MICROBIOLOGY 1 MMBVP : Food Science and Nutrition CONTENT Sl.NO. 1 2 3 4 5 6 7 8 Sensory Evaluation: Develop Grades And Ranking Cake Making : To Understand The Changes In The Functional Properties Of Nutrients On Subject To Temperature.Role Of Emulsifiers. Strach Gelatinisation : Fermentation : To Observe The Physico-Chemical Changes During Fermentation Sugar Crystallization : Study Of Different Stages Of Sugar Crystallization Fats And Oils : Study Of Smoking Temperature Of Differnet Oils Product Development : Develop A Product,Design Thenutrition Label And Develop Marketing Sreategies Visit To An Industrial Production Site
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CMR INSTITUTE OF MANAGEMENT STUDIES (AUTONOMOUS), BANGALORE III SEMESTER M.SC., MICROBIOLOGY 3 MMB 01: MOLECULAR BIOLOGY 52 HOURS CONTENT DNA Replication :Mode of DNA replication, Initiation of ss DNA; Retroviruses and their unique mode of DNA synthesis, relationship between DNA replication and cell cycle, Inhibitors of DNA replication, DNA damage and repair-Types of DNA damage-deamination, oxidative damage, alkylation, pyrimidine dimmers, Repair pathways-Photo reactivation, excision repair, post replication repair, SOS repair, methyl directed mismatch repair, very short patch repair. Structural and Functional aspects :rRNA, tRNA, mRNA, and their functions, Initiator and elongator class tRNA,ribosomes-characteristics, structural aspects and properties, nucleosomes, regulation of rRNA and tRNA synthesis Transcription :RNA polymerase in prokaryotes, its molecular composition and role of each component of RNA polymerase, Mechanism of transcriptioninitiation, elongation and termination. Global regulatory responses, heat shock response, stringent response, regulation by ppGpp and cAMP, Inhibitors of RNA synthesis and their mode of action, Polycistronic and monocistronic RNAs, Control of transcription by interaction between RNA polymerases and promoter regions, use of alternate sigma factor, Controlled termination- attenuation and anti-termination. Mechanism of transcription in Eukaryotes. Translation :Basic features of genetic code, amino acid activation, ribosome binding site on mRNA and corresponding site on rRNA, peptidyltransferase activity of 23s rRNA, mechanism of initiation, elongation and termination, Inhibitors of protein synthesis, synthesis of transport proteins on membrane bound ribosome, signal hypothesis, In Vitro transcription and system translation. Regulation of Gene Expression in Prokaryotes :Trancriptional control, enzyme induction and repression, constitive synthesis of enzymes. The Operon concept, catabolic repression, instability of bacterial RNA, inducers and corepressors, Negative gene regulation- E.coli Lac operon, Positive regulationE.coli Ara operon, Regulation by attenuation Trp operon, Antitermination- N protein and nut sites, DNA binding protein, enhancer sequences, identification of protein binding sites on DNA, Maturation and processing of RNAmethylation, cutting, trimming of rRNA, capping, polyadenylation and splicing of mRNA, cutting and modification of tRNA degradation system, catalytic RNA, GI and GII intron splicing.
NO OF HRS
13
10
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CMR INSTITUTE OF MANAGEMENT STUDIES (AUTONOMOUS), BANGALORE III SEMESTER M.SC., MICROBIOLOGY 3 MMB 02: RECOMBINANT DNA TECHNOLOGY 52 HOURS CONTENT Restriction Endonucleases : Types, nomenclature, properties and applications. Cloning vectors: general characteristics, PBR, PUC series. Phagmids, cosmids. Phage vectors: M13, Lambda, PAC. Yeast vectors: shuttle vector, 2 , yeast integrative chromosomes and their types, YAC. High Capacity Vectors: BAC,MAC,HAC. Expression vectors: general characteristics, promoter probe vectors, terminator probe vectors, ribosome binding site vectors, expression cassettes, gene cartridges. Plant vectors: Ti and Ri plasmids. Liagation And Transforamtion : Ligation Techniques: structure, types, properties of E.coli and T4 DNA ligases.Transformation: CaCl2 and electroporation.Transfection: Invitropackaging: single and double strain system. Transformation in plant and animals. Selection and Screening of Recombinants: Using antibiotics and color additives: replica plate techniques, blue- white screening, X-gal, ONPG. phage markers: Spi, pI, yeast markers: URE, LUE, SUP4, red-white screening. etc., Other methods: colony hybridization, blotting, probes. Expression Of Recombinant Proteins : Basic criteria, selection of promoter, terminator, and ribosome binding site sequences, fusion protein tags, protease cleavage sites and plasmid copy number in prokaryotes. Brief account on expression of recombinant proteins in eukaryotes.
NO OF HRS
10
10
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References: 1) S.B Primrose, R.M. Twyman and R.W Old Principles of Gene Manipulation. 6th edition 2001. Blackwell Science. 2) James D. Watson, Michael Gilman, Jan Witkowaski Mark Zoller: Recombinant DNA, 2nd edition Scientific American books distributed by W.H. Freeman and company New York. 3) Bernard R. Glick, Jack J. Pasternak Molecular biotechnology principle and applications Recombinant DNA. 3rd edition ASM Press Washington, D.C 2003. 4) David A. Micklos, Greg. A. Freyer with David.A. Crotty DNA Science a first course 2 nd edition I.K.International Pvt Ltd 2005. 5) T.A. Brown GENOME 3 GS Garland Science Taylor and Francis Group. New York and London 2007. 6) M.J. McPherson, S,G Moller PCR BIOS Scientific publishers Ltd 2000. 7) D.M. Glover and B.D Hames. DNA Cloning 3 a practical approach Indian edition Complex Genomes 2nd edition 2006. Oxford University Press. 8) S.B Primrose, R.M. Twyman Principles of Gene Manipulation and Genomics. 7 th edition 2006. Blackwell publishing. 9) Gupta P.K. Genetics a text book for university students 3rd edition 2002-2003. 10) S.S. Purohit Biotechnology fundamentals and applications 4th edition , Agrobios (india) 2005 11) Singh B.D Fundamentals of Genetics 2nd edition. 12) J.Sambrook, E.F.Fritsch and T. Maniatis Molecular Cloning a laboratory manual 2nd edition cold spring harbor laboratory press 1989. 13) Issac S. Kohane, Alvin T.Kha and Atul J. Butte Microarray for an integrative genomics. Published in india by Ane Books 2003. 14) Jeremy W Dale, Simon F Park Molecular Genetics of Bacteria 4th edition John WILEY and sons Ltd 2004. 15) Jack G. Chirikjian, Biotechnology theory and techniques vol-1 and vol-2 genetic engineering separation technology, Janes and Bartlett publishers.
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CMR INSTITUTE OF MANAGEMENT STUDIES (AUTONOMOUS), BANGALORE III SEMESTER M.SC., MICROBIOLOGY 3 MMB 03: ENVIRONMENTAL MICROBIOLOGY 52 HOURS CONTENT Aerobiology: droplet nuclei, aerosol,assessment of air quality- Anderson ,Rotorod, Burkard, solid and liquid impingement method and filters. Brief account of transmission of air borne microbes- Viruses ,Bacteria and Fungi .Microbiology of indoor and outdoor environments, disease caused by air borne microbes and allergens(Infections, their detection and enumeration). Biohazards caused by endotoxins.
NO. OF HRS
10
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11
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CMR INSTITUTE OF MANAGEMENT STUDIES (AUTONOMOUS), BANGALORE III SEMESTER M.SC., MICROBIOLOGY 3 MMB 04: FOOD MICROBIOLOGY
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CMR INSTITUTE OF MANAGEMENT STUDIES (AUTONOMOUS), BANGALORE. III SEMESTER M.SC., MICROBIOLOGY 3 MMB 06: ENVIRONMENTAL AND FOOD MICROBIOLOGY NO. 1 TITLE OF THE EXPERIMENT Quantitative and qualitative analysis of microorganisms in out door and indoor air. 2 3 4 Quantitative and qualitative analysis of water. Study of microflora of industrial wastes and effluents. Study of milk and milk products.
1. 2. 3. 4. 5.
5 6 7 8 9 10 11 12
Methylene blue reductase test. Starch test. Lech test. Alcohol test. Formalin test. Litmus milk test Estimation of Lactic acid in milk. Study of fruit juices, squashes and beverages. Microbiological Examination of Flour. Study of Fermented foods. Microbiological examination of various dairy products Determination of Dissolved oxygen content of the water. Determination of biological oxygen demand of the water. Enumeration of bacteria in raw and pasteurized milk
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SYLLABUS IV SEMESTER
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NO. OF HRS 4 9
Decomposition of organic matter: by Microbes-cellulose, 48roteins48n48e4848, lignin, starch and pectin. Biological metabolism: Mineralization and immobilization of nitrogen, nitrification, denitrification, symbiotic nitrogen fixation(Rhizobium, Frankia), Non symbiotic nitrogen fixation( Azotobacter, Azosprillium): Nitrogenase enzymes, nif gene. Role of nodulin genes in nodule development and symbiosis. Plant Microbe Interactions: Mutualism, Commensalisms, parasitism, ammensalisms, synergism, predation Microbiology of Rhizosphere- Microbes in the rhizosphere and rhizoplane, methods of enumeration, rhizosphere effects, factors influencing rhizosphere microbes. Biofertilizers- Cultivation and mass production of bioinoculants- PGPR,Azotobacter, Rhizobium, Azospirillum, Cyanobacteria, Phosphate solubilising bacteria, carrier based inoculants. Mycorrhiza and its types, methods of application and agronomic importance.Microbiology and biochemistry of biogas production Biopesticides: types and application Host- parasite interactions: Structural and biochemical defence mechanisms. Production of phytoalexins; involvement of elicitors; role of R and r genes in disease development.SAR( Systamic acquired resistance). Plant diseases: 1. Diseases caused by Fungi( Symptomatology, etiology and control) a) Wilt diseases b) Downy mildews grapes c) Powdery mildews of cucurbits d) Rusts of coffee e) Smuts of sorghum 2. Diseases caused by Bacteria(Symptomatology, etiology and control) a) Bacterial Wilt b) Bacterial blight of rice c) Angular leaf spot of cotton d) Citrus canker 3. Mycoplasmal diseases( Sandal spike, grassy shoot of sugar cane) 4. Viral Diseases(Symptomatology, etiology and control) a) Tobacco Mosaic Diseases b) Banana Bunchy top c) Cucumber mosaic d) Cowpea Mosaic 5. Diseases caused by viroids, potato spindle tuber viroid Post harvest diseases and control measures
10 5
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References 1. Charudattan R. 1982. Biological control of weeds with palnt pathogens. John Wiley and sons 2. George N. Agrios.2000. Plant Pathology. 4th Ed.Academic press 3. Noriss.J.R ans Pettiper.G.L.1987.Essays in agricultural and food microbiology. John Wileyt and sons. Singapore 4. Rangaswami.G and D.J. Bagyaraj.1998. Agricultural Microbiology. 2nd Ed. PHI. India 5. Singh. R.S. 1997. Introduction to principles of plant pathology.3rd edition. Oxford and TBH 6. Subba Rao. N.S. 1995. Soli Microorganisma and plant growth. Oxford and IBH 7. Sylvia.D.M, Jeffery.J.Fuhrmann. Peter.G. hartel, david.A. Zuberber.1997.Principles and applications of soil Microbiology 1st ed. Prentice Hall. 8. Subbarao. N.S and Dommergues.Y.R.1998. Microbial interactions in agriculture and forestry. Science publishers. 9. Richard.E, Issacson, marry.E and Torrece.2005. Microbial food safety in animal agriculture. Current topics. Blackwell publishers. 10. M.K.Sateesh.2010. Recombinant DNA technology. Comprehensive Biotechnology-V. New Age International Publishers. 11. M.K.Sateesh. 2010. Immunology and Plant Biotechnology. New Age International Publishers 12. Shivakumar.M.2004. Introduction to soil and Agricultural Microorganisms. Himalaya Publishing house 13. Biswas.P.K. 2008. Agricultural Microbiology. Dominant Publishers 14. Markandy.D.K.2006. Agricultural applications of Microbiology. APH publishing corporation 15. SubbaRao.N.S.1982 Advances in Agricultural Microbiology. Butterworth- Heinemann publishers. 16. Rangaswami.G and Bagyaraj.D.J . 2002. Agricultural Microbiology.RHI Publishers. 17. Raina.M.Maier.2002. Environmental Microbiology. 18. P.D.Sharma.2006. Plant pathology. Alpha Science International. 19. Sharma.P.G. 2006. Plant Pathology. Rastogi Publication.
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CMR INSTITUTE OF MANAGEMENT STUDIES (AUTONOMOUS), BANGALORE IV SEMESTER M.SC., MICROBIOLOGY 4 MMB 02: MEDICAL MICROBIOLOGY 52 HOURS CONTENT Medically Important Microorganisms: Classification of medically important microorganisms. Normal flora of human body and its significance, tissue trophism. Infections; Characteristics And Types : Portals of entry. Mechanism of host defense. Evasion of host defenses, toxigenesis- bacterial toxins and its types. Bacterial Diseases : Symptomatology, laboratory diagnosis, etiology and control of bacterial infection by Staphylococcus, Pneumococcus, E.coli, Salmonalla, Neisseria, Corynebacterium, Clostridium, Vibrio, Yersinia, Haemophilus, Mycobacterium, Spirochaetes, Chlamydiae and Mycoplasma. Protozoan infections- malaria and filaria. Fungal Diseases : Pathogenisis, laboratory diagnosis and control of pathogenic fungisuperficialand systemic mycoses, Aspergillosis, Candidiasis, Mycotoxins. Viral And Prion Diseases : Diseases caused by Pox virus, Herpes virus, Picorna virus, Orthomyxo virus, Paramyxo virus, Rhabdo virus, Hepatitis virus, Oncogenic viruses, HIV, Dengue, Encephalitis. Prion infection: CJD, Kuru. Microbial Diagnostics And Antimicrobial Therapy : Collection and transport of clinical specimens, Immuno and molecular diagnostic methods. Antibacterial, antifungal and anti protozoal drugs, methods of testing drug sensitivity, antibiotic assay in fluids. Vaccines and vaccination schedule; Nosocomial infections- diagnosis and control. References: 1. Ananthanarayana.R and C.K.Jayaram Panicker, Text book of Microbiology , 4th edition, 2002 2. Jawetz E, Melnick J.L., Adelberg E.a. Review of Medical Microbiology, 6th edition, 2005 3. Mackie and McCartney , Medical Microbiology vol I, Microbial Infection vol 2, practical medical microbiology, 3rd edition, 1996 4. Murray , Medical Microbiology, 4th edition, 2003. 5. Baily and Scott, Diagnostic Microbiology, 5th edition,2006 6. David Greenwood, Medical Microbiology, 5th edition, 2005 7. Hurst, Microbiology for Health Sciences , 6th edition,2002 8. Pelczar, Microbiology,4th edition,2000 NO. OF HRS 5 10
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CMR INSTITUTE OF MANAGEMENT STUDIES (AUTONOMOUS), BANGALORE IV SEMESTER M.SC., MICROBIOLOGY 4 MMB 03: INDUSTRIAL MICROBIOLOGY 52 HOURS CONTENTS Scope Of Industrial Microbiology : Definition of industrial microbiology, Historical developments, Economics of microbial biotechnology; Biotechnological innovation in the chemical industry. Biocatalyst in organo chemical regulation, P/O quotients, metabolic overproduction and growth efficiency. Strain Improvement : Criteria for selection and strategies for strain improvement, maintenance and containment of recombinant organisms. Microbial Products Of Commercial Use : Industrial production of organic acids(citric acid, acetic acid, gluconic acid), amino acids (lysine, glutamic acid), solvents (ethanol, glycerol), antibiotics (penicillin, streptomycin, 51roteins51n51e), microbial polysaccharides (xanthan), polyesters (3 hydoxyalcanoate), Hormones (insulin), production of antibodies, Steroids transformation, anticholesterol compound (lovastatin). Microbial Enzymes And Microbes In Mineral Recovery : Industrial production of microbial enzymes (amylase, protease, cellulose), use of enzymes in starch processing, cheese making, textile industries, detergent industries, leather industries, breweries, pharmaceuticals and therapeutics.Metal leaching by bacteria from ores ( copper, iron, uranium, gold) Probiotics :Industrial production of Lactobacillus, Bifidobacterium, Streptococcus sps, Industrial production of lactic acid, acidophilus milk, yoghurt. Production of Cheese. Immobilization Of Enzymes : Definition and concept of immobilization of enzymes and whole cell, Immobilization techniques- adsorption, cross-linkage, covalent modification, entrapment and encapsulation. Potential benefits of immobilization, Two dimensional separation of total cellular proteins, Protein microarray. References: 1) Barwart,G. J (1989).Basic Food Microbiology,Chapman and Hall,New York 2) El.mansi,E.MT amd Bryce,C.F.A (2002),fermentation microbiology and biotechnology Taylor and Francis,London NO OF HRS 3
13
15
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Fermentation Tehcnology : Introduction, concepts and scope. Isolation and purification industrial important organisms. Culture collection institutions. Fermentors : Design, components, body construction and basic functions. Different 13 types of fermentors, aeration and agitation systems, baffles, sterilization of fermentor medium, sterilization of air supply, temperature control. Aseptic inoculation and sampling methods. Scale up of fermentation process: parameter used in scale up and problems associated, solid state fermentation (Koji fermentor). Fermentation Media : Media formulation strategies, sources of carbon and nitrogen, 8 vitamins and minerals, role of buffers, precursors, inhibitors and inducers, Antifoaming agents. Substrates for solid state fermentation. Fermentation In Batch And Continuous Cultures :Batch growth, microbial growth 9 kinetics and thermal death of microorganisms. Effects of environments: temperature, P H and high nutrients concentration, methods of monitoring microbial growth. Continuous culture systems : concepts of Newtonian and Non-Newtonian fluid, plastic fluids, apparent viscosities. Downstream Processing : Objectives and criteria, foam separation, cell disruption 13 methods, centrifugation, precipitation methods, filtration, liquid extraction, membrane filtration, chromatography techniques, drying devices, crystallization. Solvent recovery.2D 52roteins52n of total cellular 52roteins. Quality Control: Quality control of fermented foods, chemical and microbial analysis, 5 FDA regulation for fermented foods and pharmaceutical products. References: 1. E.M.T. El-Mansi, C.F.A. Bryce, A.L.Demain A.R.Allman(2007) Fermantation microbiology and biotechnology 2nd edition Taylor and Francies group Boca Raton London New York.
No. Of HRS 4
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CMR INSTITUTE OF MANAGEMENT STUDIES (AUTONOMOUS) IV SEM M.Sc., MICROBIOLOGY 4 MMB 06P: MICROBIAL AND FERMENTATION TECHNOLOGY NO. 1 2 3 4 TITLE OF THE EXPERIMENT Entrapment of enzymes and microbial cells using alginate methods. Production of citric acid from A.niger, T. viridae. Production of Penicillin and ethanol by small scale solid state fermentation. Detection and quantification of pigments produced by certain fungi and bacteria (P. chrysogenum, S. mercescens, Psuedomonas spp.) Detection and quantification of siderophores produced by Psuedomonas spp.
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CMR INSTITUTE OF MANAGEMENT STUDIES (AUTONOMOUS) IV SEMESTER M.SC., BIOTECHNOLOGY 2 MB07VP: MOLECULAR DIAGNOSTICS VALUE ADDED COURSE NO 1 2 3 4 5 6 7 8 TITLE OF THE EXPERIMENT DNA extraction from peripheral blood Quality and quantity checking of nucleic acids RNA extraction from peripheral blood RFLP analysis Polyacrylamide gel electrophoresis Western blotting Molecular detection of any two human genetic diseases Bioinformatics tools for genome and proteome analysis
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