Electrical Impedance and Colorimetric Measurements
Electrical Impedance and Colorimetric Measurements
Electrical Impedance and Colorimetric Measurements
and
Colorimetric Measurements
Joanna Ellis,
MLS(ASCP)
Objectives
Cite the electrical impedance
principle of cell counting.
Identify and interpret microcytic and
macrocytic RBC histograms
Define coincidence
Identify the cell populations
represented on a 3-part differential
WBC histogram
History
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Prior to the 1950s blood cell counts were
performed by manual methods:
Counting Chambers
RBC/Platelet
Chamber
WBC Chamber
Differential
Chamber
Reticulocyte
Channel
RBC/Platelet Chamber
Particle Size
Platelet
36-360 fL
Bimodal Histogram
Bimodal peak can be seen in situations such
Cold agglutinin disease
Post-transfusion
Post-treatment of IDA
Coincidence
WBC Chamber
WBCs
Lysing
agent
Hgb
released
Aperture in
electric
field
Colorimetric
Measurements
Hemoglobin is often determined by a colorimetric method.
Imidazole
Non-cyanide reagent with color change and read at
540nm
Instruments
Abbott CELL-DYN Sapphire
Sodium Lauryl Sulfate
Non-cyanide reagent with color change and read
spectrophotometrically
Instruments
Sysmex XT and XE
Lysing agent converts free hemoglobin to
cyanmethemoglobin and read spectrophotometrically at
540nm.
Instruments
Advia 120
Some Beckman Coulters
Three-part Differential
WBC Histogram
Lymphocytes: 35-92L
Mononuclear Cells: 92-152 fL
Granulocytes: 152-450 fL
References