Anthrax
Anthrax
Anthrax
Death Introduced
Blood stream
Release
Vegetative Forms
BImal K Das, Microbiology, AIIMS
Clinically three forms of Human anthrax occur
A. Cutaneous anthrax
B. Pulmonary anthrax
C. Intestinal anthrax
Cutaneous anthrax
Rarely intestinal anthrax
Intestinal Anthrax
Haemorrhagic mediastinitis
CUTANEOUS ANTHRAX
INTESTINAL ANTHRAX
PULMONARY ANTHRAX
Putrefying carcasses
Blood, tissue and hide
Culture on selective medium
Soil sample from the areas where the carcass as lying
Serological assay
ELISA: based on anthrax toxin ( PA, LF and EF) for routine confirmation and
vaccine response)
Molecular techniques ( Only in the referral laboratories):
- RFLP
- PCR Fingerprinting
Animal Inoculation: Guinea pig and mice inoculation
Children Ciproflox
20-30mg/kgbodywt ivX60days Penicllin G 50,000 u/kg X 60 days
Vaccine for humans: ( avirulent and nonencapsulated) sublethal amounts of the toxin
produced
- Professionals ( Veternarians, butcher, Zoo keeper, Wild life workers, Forest guards)
- Military personnels