Biology:NFYA
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Nuclear transcription factor Y subunit alpha is a protein that in humans is encoded by the NFYA gene.[1][2]
Function
The protein encoded by this gene is one subunit of a trimeric complex NF-Y, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes.[3] Subunit NFYA associates with a tight dimer composed of the NFYB and NFYC subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the NFYA subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.[4]
NF-Y complex serves as a pioneer factor by promoting chromatin accessibility to facilitate other co-localizing cell type-specific transcription factors.[5]
NF-Y has also been implicated as a central player in transcription start site (TSS) selection in animals.[6] It safeguards the integrity of the nucleosome-depleted region and PIC localization at protein-coding gene promoters.
Interactions
NFYA has been shown to interact with Serum response factor[7] and ZHX1.[7][8] NFYA, NFYB and NFYC form the NFY complex and it has been shown that the NFY complex serves as a pioneer factor by promoting chromatin accessibility to facilitate other co-localizing cell type-specific transcription factors.[3]
References
- ↑ "One subunit of the transcription factor NF-Y maps close to the major histocompatibility complex in murine and human chromosomes". Genomics 11 (3): 630–4. November 1991. doi:10.1016/0888-7543(91)90070-U. PMID 1774067.
- ↑ "Role of the CCAAT-binding protein CBF/NF-Y in transcription". Trends in Biochemical Sciences 23 (5): 174–8. May 1998. doi:10.1016/S0968-0004(98)01201-8. PMID 9612081.
- ↑ 3.0 3.1 "Histone-fold domain protein NF-Y promotes chromatin accessibility for cell type-specific master transcription factors". Molecular Cell 55 (5): 708–22. September 2014. doi:10.1016/j.molcel.2014.07.005. PMID 25132174.
- ↑ "Entrez Gene: NFYA nuclear transcription factor Y, alpha". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4800.
- ↑ "Histone-fold domain protein NF-Y promotes chromatin accessibility for cell type-specific master transcription factors". Molecular Cell 55 (5): 708–22. September 2014. doi:10.1016/j.molcel.2014.07.005. PMID 25132174. [verification needed]
- ↑ "NF-Y controls fidelity of transcription initiation at gene promoters through maintenance of the nucleosome-depleted region". Nature Communications 10 (1): 3072. 2019-07-11. doi:10.1038/s41467-019-10905-7. PMID 31296853. Bibcode: 2019NatCo..10.3072O.
- ↑ 7.0 7.1 "Identification of proteins that interact with NF-YA". FEBS Letters 460 (1): 41–5. October 1999. doi:10.1016/S0014-5793(99)01311-3. PMID 10571058.
- ↑ "Human ZHX1: cloning, chromosomal location, and interaction with transcription factor NF-Y". Biochemical and Biophysical Research Communications 261 (3): 614–21. August 1999. doi:10.1006/bbrc.1999.1087. PMID 10441475.
Further reading
- "The molecular biology of the CCAAT-binding factor NF-Y". Gene 239 (1): 15–27. October 1999. doi:10.1016/S0378-1119(99)00368-6. PMID 10571030.
- "Evolutionary variation of the CCAAT-binding transcription factor NF-Y". Nucleic Acids Research 20 (5): 1087–91. March 1992. doi:10.1093/nar/20.5.1087. PMID 1549471.
- "Intron-exon organization of the NF-Y genes. Tissue-specific splicing modifies an activation domain". The Journal of Biological Chemistry 267 (13): 8984–90. May 1992. doi:10.1016/S0021-9258(19)50377-5. PMID 1577736.
- "A cDNA encoding a human CCAAT-binding protein cloned by functional complementation in yeast". Proceedings of the National Academy of Sciences of the United States of America 88 (5): 1968–72. March 1991. doi:10.1073/pnas.88.5.1968. PMID 2000400. Bibcode: 1991PNAS...88.1968B.
- "Purification and molecular cloning of the "A" chain of a rat heteromeric CCAAT-binding protein. Sequence identity with the yeast HAP3 transcription factor". The Journal of Biological Chemistry 265 (36): 22480–6. December 1990. doi:10.1016/S0021-9258(18)45730-4. PMID 2266139.
- "Dominant negative analogs of NF-YA". The Journal of Biological Chemistry 269 (32): 20340–6. August 1994. doi:10.1016/S0021-9258(17)31997-X. PMID 8051128.
- "Functional interaction between the DNA binding subunit trimerization domain of NF-Y and the high mobility group protein HMG-I(Y)". The Journal of Biological Chemistry 272 (49): 30880–8. December 1997. doi:10.1074/jbc.272.49.30880. PMID 9388234.
- "Biochemical characterization of the NF-Y transcription factor complex during B lymphocyte development". The Journal of Biological Chemistry 273 (29): 18220–9. July 1998. doi:10.1074/jbc.273.29.18220. PMID 9660784.
- "Interaction between the two ubiquitously expressed transcription factors NF-Y and Sp1". Gene 234 (1): 61–9. June 1999. doi:10.1016/S0378-1119(99)00180-8. PMID 10393239.
- "Human ZHX1: cloning, chromosomal location, and interaction with transcription factor NF-Y". Biochemical and Biophysical Research Communications 261 (3): 614–21. August 1999. doi:10.1006/bbrc.1999.1087. PMID 10441475.
- "Identification of proteins that interact with NF-YA". FEBS Letters 460 (1): 41–5. October 1999. doi:10.1016/S0014-5793(99)01311-3. PMID 10571058.
- "BRCA1 regulates GADD45 through its interactions with the OCT-1 and CAAT motifs". The Journal of Biological Chemistry 277 (10): 8061–7. March 2002. doi:10.1074/jbc.M110225200. PMID 11777930.
- "An alternative model of H ferritin promoter transactivation by c-Jun". The Biochemical Journal 363 (Pt 1): 53–8. April 2002. doi:10.1042/0264-6021:3630053. PMID 11903046.
- "Transcription factor NF-Y regulates differentiation of CaCo-2 cells". Archives of Biochemistry and Biophysics 407 (1): 39–44. November 2002. doi:10.1016/S0003-9861(02)00436-8. PMID 12392713.
- "Synergistic regulation of human cystathionine-beta-synthase-1b promoter by transcription factors NF-YA isoforms and Sp1". Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression 1579 (2–3): 73–80. December 2002. doi:10.1016/s0167-4781(02)00509-2. PMID 12427542.
- "Interactions between p300 and multiple NF-Y trimers govern cyclin B2 promoter function". The Journal of Biological Chemistry 278 (9): 6642–50. February 2003. doi:10.1074/jbc.M210065200. PMID 12482752.
- "The NFY transcription factor inhibits von Willebrand factor promoter activation in non-endothelial cells through recruitment of histone deacetylases". The Journal of Biological Chemistry 278 (10): 8385–94. March 2003. doi:10.1074/jbc.M213156200. PMID 12511565.
External links
- NFYA+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH)
- FactorBook NF-YA
This article incorporates text from the United States National Library of Medicine, which is in the public domain.
![]() | Original source: https://en.wikipedia.org/wiki/NFYA.
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