Triangle Biomed ATP1 Operators-Manual PDF

Pub No.
: OM ATP
June 2013, Version 5.6
ATP1-120; ATP1-220
ATP1-T-120; ATP1-T-220
Histology Innovation for a NEW Generation
Operators Manual
ATP Tissue Processor

Catalog #s
ATP1-120, ATP1-220
ATP1-T-120, ATP1-T-220
WELCOME
Thank you for selecting the ATP Automated Tissue Processor. This instrument was
carefully designed to be easy to use, safe to operate and capable of producing
consistent, quality results. The embedded Personal Computer affords you the flexibility
and reproducibility required by the modern laboratory. The built-in software safety
features protect you (the operator) while its internal electronic sensors protect the
instruments critical components. The ability to optimize each step of every program will
allow you to create programs that yield excellent reproducible results consistently.
The employees of TBS thank you for your support. Feel free to call TBS
customer service at 919-384-9393 or e-mail us for support at
[email protected].
TBS Corporate Headquarters

Phone:
919-384-9393
Fax:
919-384-9595
E-mail:
[email protected]
Web:
http://www.trianglebiomedical.com
Product Service Department

Phone:
919-384-9393
E-mail:
[email protected]
Copyright 2013 General Data Healthcare, Inc.
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TABLE OF CONTENTS
INTRODUCTION ..................................................................................................... 1
CHAPTER 1 GENERAL INFORMATION ............................................................. 2
1.1 Regulatory Compliance .................................................................................. 2
1.2 Patents, Trademarks and Specifications ........................................................ 3
1.3 Symbols and Conventions .............................................................................. 3
1.4 Abbreviations and Units of Measure ............................................................... 4
1.5 Safety Precautions ......................................................................................... 5
CHAPTER 2 UNPACKING, PACKING AND TRANSPORTING ......................................... 7
2.1 Unpacking ...................................................................................................... 7
2.2 Packaging and/or Preparation for Transport................................................... 8
2.3 Transportation ................................................................................................ 8
CHAPTER 3 INTRODUCTION TO THE ATP PROCESSOR.......................................... 9
3.1 General Features ........................................................................................... 9
3.2 Front View .................................................................................................... 10
3.3 Rear Panel & Floppy Drive ........................................................................... 11
Floppy Drive ....................................................................................................... 11
CHAPTER 4 REAGENTS................................................................................... 12
4.1 Compatible Reagents ................................................................................... 12
4.2 Incompatible Reagents ................................................................................. 13
CHAPTER 5 INSTALLATION AND OPERATIONAL QUALIFICATION................................. 14
CHAPTER 6 PREPARATION FOR OPERATION .............................................. 15
6.1 Filling Reagent Bottles.................................................................................. 15
6.2 Filling Paraffin Bottles ................................................................................... 16
6.3 Installing Charcoal Bottles ............................................................................ 17
CHAPTER 7 FIRST RUN PROGRAMMING ...................................................... 18
7.1 Introduction ................................................................................................... 18
7.2 Language ..................................................................................................... 18
7.3 Main Menu .................................................................................................... 19
7.4 Reagent Labels ............................................................................................ 20
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7.5 Program Label .............................................................................................. 21

7.6 Hour and Date .............................................................................................. 22
7.7 Purge Reagents Limits Setting ..................................................................... 23
7.8 CCW (Paraffin Oven) Temperature Setting .................................................. 23
7.9 Charcoal Filter Limit Setting ......................................................................... 23
7.10 WPC (Wax Purification Cycle) .................................................................... 24
Activation / Deactivation ........................................................................................ 24
7.10.1 Manual Start of the WPC ......................................................................... 25
7.11 RMS Activation ........................................................................................... 25
CHAPTER 8 PROGRAMMING PROCESSES ................................................... 26
8.1 Programming Processes .............................................................................. 26
Reagent ............................................................................................................. 27
Time ................................................................................................................... 27
Temp.................................................................................................................. 27
P/V ..................................................................................................................... 27
Agit .................................................................................................................... 28
8.2 Process # 13: Reversed ............................................................................... 28
CHAPTER 9 REAGENT MANAGEMENT SYSTEM (RMS) ............................... 29
9.1 Introduction ................................................................................................... 29
9.2 Choosing the RMS Mode ............................................................................. 29
9.2.1 RMS Single Mode Description and Example ............................................. 31
9.2.2 RMS Group Mode Description and Example ............................................. 32
Example Group Mode Sequence ....................................................................... 32
9.3 RMS Programming Reagents Definition .................................................... 35
Single Mode ....................................................................................................... 35
Group Mode ....................................................................................................... 35
9.4 RMS Programming Limits Definition .......................................................... 36
Single Mode ....................................................................................................... 36
Group Mode ....................................................................................................... 37
9.5 RMS Counters Total Reset ........................................................................ 37
RMS Single Counter Reset ............................................................................. 37
9.6 RMS On / Off ................................................................................................ 38
9.7 Printing Menu ............................................................................................... 38
CHAPTER 10 NORMAL OPERATION ............................................................... 40
10.1 Loading Samples into the Basket ............................................................... 40
10.2 Checks and Procedures before Operating ................................................. 40
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10.3 Selecting a Program ................................................................................... 41

10.4 Setting the Program Completion Time/Date ............................................... 41
Setting Delay ..................................................................................................... 42
Programming Completion Times ....................................................................... 42
Midweek holidays .............................................................................................. 43
Starting from step other than the first ................................................................. 43
Runtime Test Aborting Run................................................................................ 43
10.5 Starting a Procedure................................................................................... 44
10.6 Interrupting the Process ............................................................................. 45
10.7 Opening the Retort During a Program ........................................................ 46
10.8 Process Completion ................................................................................... 46
10.9 Cleaning the Retort (Clean Cycle) .............................................................. 47
CHAPTER 11 ALARMS...................................................................................... 49
11.1 Managing Alarms ....................................................................................... 49
Non-Blocking Alarms ......................................................................................... 49
Blocking Alarms ................................................................................................. 49
11.2 Non-Blocking Alarms .................................................................................. 50
1 - BLACK OUT ................................................................................................. 50
2 - RETORT T LOW ......................................................................................... 50
3 - SHORT DRAIN ............................................................................................. 51
4 - P/V TIMEOUT ............................................................................................... 51
5 - FULL FILL P ................................................................................................. 51
6 - FULL FILL S ................................................................................................. 51
7 - FILE NOT FOUND ........................................................................................ 52
8 - LONG DRAIN ............................................................................................... 52
9 - T WAX LOW ................................................................................................ 52
11.3 Blocking Alarms .......................................................................................... 52
11 - FILL NO VACUUM...................................................................................... 52
12 - FILL TIME OUT .......................................................................................... 52
13 - OVER FILL S .............................................................................................. 53
14 - OVER T RETORT ..................................................................................... 53
15 - TSC OUT .................................................................................................... 53
16 - SHORT FILL ............................................................................................... 53
17 - VR OUT ...................................................................................................... 53
18 - FILE NOT FOUND ...................................................................................... 53
19 - RETORT T LOW ....................................................................................... 54
20 - WAX T LOW .............................................................................................. 54
21 - OVER T WAX ............................................................................................ 54
22 - TSW OUT ................................................................................................... 54
23 - PRESSURE N.R. ........................................................................................ 54
24 - DRAIN NO PRESS ..................................................................................... 54
25 - DRAIN TIME OUT ...................................................................................... 55
26 - RET. NOT EMPTY...................................................................................... 55

27 - OVER FILL P .............................................................................................. 55
30 - COM ERROR [FRAME] .............................................................................. 55
31 - COM ERROR [PARITY] ............................................................................. 55
32 - COM ERROR [OVERRUN] ........................................................................ 55
33 - COM ERROR [TIMEOUT] .......................................................................... 56
39 - COM ERROR [LINE OR PORT DOWN] ..................................................... 56
40 - ELODEV LACK ........................................................................................... 56
50 - OVER PRESSURE ..................................................................................... 56
51 - OVER VACUUM ......................................................................................... 56
52 PS BREAK HIGH .................................................................................... 56
53 PS BREAK LOW ..................................................................................... 57
11.4 Alarms Historical Archive ............................................................................ 57
11.5 Instrument Reset ........................................................................................ 57
CHAPTER 12 SERVICE MENU ......................................................................... 59
12.1 End User Service Tests .............................................................................. 59
Vacuum test ....................................................................................................... 59
Pressure Test .................................................................................................... 60
Retort Heating.................................................................................................... 60
Runtime Test ..................................................................................................... 60
Edit Setup .......................................................................................................... 60
Reset Flag ......................................................................................................... 60
Alarms Reset ..................................................................................................... 60
12.2 Service Menu Abbreviation List .................................................................. 61
12.3 Service Assistance ..................................................................................... 61
CHAPTER 13 SAFETY FEATURES .................................................................. 62
13.1 Protection against Overheating .................................................................. 62
13.2 Protection against Excess Pressure ........................................................... 62
CHAPTER 14 INSTRUMENT MAINTENANCE .................................................. 63
14.1 Daily Maintenance ...................................................................................... 63
14.2 Monthly Maintenance ................................................................................. 63
14.3 Paraffin Wax Purification Cycle .................................................................. 63
14.4 Charcoal Filters Replacement .................................................................... 64
14.5 Retort Lid Gasket Replacement ................................................................. 64
14.6 Fuse Replacement ..................................................................................... 65
CHAPTER 15 ACCESSORIES .......................................................................... 66
15.1 Printer ......................................................................................................... 66
15.2 Remote Alarm and Auto-dialer ................................................................... 67
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APPENDIX 1 ACCESSORIES AND SPARE PARTS ......................................... 68

APPENDIX 2 ATP TECHNICAL DATA SHEET .............................................. 69
APPENDIX 3 ATP FEATURES ....................................................................... 70
APPENDIX 4 ATP PROGRAM MENU FLOW ................................................ 71
CONTACT INFORMATION ................................................................................... 72
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INTRODUCTION
Thank you for purchasing an ATP. The instrument was designed by experienced
engineers with the input of service and laboratory technicians. The number one
priority was to produce the most dependable tissue processor on the market. After
getting to know your instrument, we trust that you will agree. As always, TBS
sincerely welcomes your suggestions for any improvements.
CHAPTER 1 GENERAL INFORMATION

Before using the instrument, please make certain that you carefully read this manual.
Pay particular attention to the precautions that must be taken for user and product
safety.
The ATP is an automatic tissue processor for laboratory use. It should only be
used for routine processing of histological tissue samples.
To avoid potential instrument damage, do not use different reagents from those
specified in Chapter 4.
The instrument should only be operated by qualified technicians.
The warranty applies only if the instrument is used in the correct manner and in
accordance with the information and advice provided herein. The manufacturer
declines all responsibility for possible damages to persons and/or objects due to
improper or inexperienced use of the instrument.
1.1 REGULATORY COMPLIANCE
1.2 PATENTS, TRADEMARKS
AND
SPECIFICATIONS
Prices and specifications are current at time of publication; however, they are subject
to change without notification. Please contact our North Carolina office or one of our
distributors for current prices and availability. ATP and Innovations for Science
are trademarks and TBS is a registered trademark of General Data Healthcare,
Inc., Cincinnati, Ohio, U.S.A. The ATP has certain features that are covered under
Patent # US 6,780,380 B2.
1.3 SYMBOLS
AND
CONVENTIONS
Do not use free flame near the instrument
No smoking near the instrument
Recyclable
High voltage
Important safety notice
Important operation information
1.4 ABBREVIATIONS
ABBREVIATIONS
AND
UNITS
OF
MEASURE
A/D
Analog/Digital Converter
CCW
CdP
MdC
MV
PMP
PNV
PV1
SdO
SdP
SGR
VR
VT
VV1
WPC
Retort
Paraffin Wax Heating Chamber

Retort (Processing Chamber)
Control Module
Main Valve
Air Pump
Ambient Valve
Pressure Valve
Overfill Sensor
Rotating Valve Position Sensor
Reagents Management System
Rotating Valve
Vapor Trap
Vacuum Valve
Paraffin Wax Purification Cycle
Processing chamber
UNITS OF MEASURE
Bar
mBar
W
A
V
Pressure unit of measure

Millibar; 1000 mBar = 1 Bar
Power unit of measure; Watt
Resistance unit of measure; Ohm
Current unit of measure; Ampere
Voltage unit of measure; Volt
1.5 SAFETY PRECAUTIONS

Carefully follow the installation instructions:
Severe damage can result if the instrument is connected to a power supply
different from that stated in the manual and on the identification tag at the rear
of the instrument.
The instrument must NEVER be used without being connected to an
appropriate ground. If damages due to transport occur, DO NOT use
and DO NOT connect the instrument to a power source. Contact TBS
technical service.
This instrument has been designed to work 24 hours a day; for this reason and
for operative precautions, the power switch is placed at the rear of the
instrument, on the right-hand side at approximately the same height as the
screen in the front.
Always make certain to correctly engage the reagent bottles.
NEVER use ACETONE, BENZENE, or TRICHLOROETHANE.
Fixatives with mercury salts, acetic or picric acid may corrode metal. We
strongly advise against their use.
DO NOT open the Retort lid when the instrument is working without having
previously followed the instructions contained in this manual.
Use specific precautions in handling flammable reagents such as ethanol.
Use specific precautions in handling liquid paraffin waxes as they can cause
burns.
The emptying and filling of reagent bottles must only be done by qualified
personnel.
Due to the presence of flammable substances inside reagent bottles, it is

recommended to:
Avoid smoking near the instrument.
Avoid using open flames near the instrument (e.g. Bunsen burner).
DO NOT wear clothes that are prone to create electrostatic charges while
handling reagents (wool, synthetic fibers, etc.).
Contaminated reagent waste must be disposed of in accordance with all

applicable laws and ordinances.
Access to the instruments internal components is reserved only to those

specialists trained in the service of the instrument.
Always disconnect the processor from the electrical source before

accessing the electronics.
Only original spare parts sold by the manufacturer or by authorized dealers

are recommended for the instrument.
BEFORE replacing fuses, disconnect the instrument from the power

source.
DO NOT extract bottles, replace reagents or do other maintenance

operations when the instrument is operating.
CHAPTER 2 UNPACKING, PACKING AND TRANSPORTING

Before moving or transporting the instrument, it is essential to carefully read this
chapter, paying particular attention to the instrument setup instructions.
The warranty is invalid if the instrument is improperly operated. Be certain to follow
the instructions and recommendations provided by this manual. The manufacturer is
not responsible for damages resulting from improper operation or handling of the
instrument. If the crate is damaged, inform the driver and get written acknowledgment
of the damage on the delivery truck or you may forfeit any rights for damage
recovery.
Pay particular attention to the outside of the shipping container. In the event of
concealed damage, save all shipping crates and packing material. DO NOT unpack
the instrument if damage is apparent. Immediately notify the carrier of any damage
and contact the shipper to initiate any claims.
2.1 UNPACKING
The proper steps to be taken for unpacking are:

1) Open the rear of the box.
2) Remove the screws securing the top.
3) Remove screws and nails securing the wooden sides to the base of the box.
4) Lift the side walls out of the way.
5) Raise the instrument to the vertical position as you move it off of the wooden
pallet.
6) Remove the plastic layers that wrap the instrument.
7) Remove the nylon bag that contains the instrument.
8) Carefully check the external condition of the instrument. In the event of
concealed damage, DO NOT plug in the instrument. Immediately notify the
carrier and promptly contact the seller.
9) For the setup of the instrument, see the specific chapter in this manual.
10) Save the box and internal packaging for future shipment.
2.2 PACKAGING
AND/OR
PREPARATION
FOR
TRANSPORT
To transport the instrument, perform the following steps:
1) Remove all reagents (paraffin included) from their bottles.

2) Remove and close, with the proper threaded cap, the charcoal filter bottles.
3) Transport the charcoal filter bottles separately from the instrument, wrapped
and securely closed in a protective nylon bag.
4) If the original box has been saved, follow the unpacking instructions in the
reverse order, using all the interior packaging to avoid serious damage to the
instrument during shipping.
5) Using the original box or another box adapted to carry the instrument
horizontally, lay the carton on its back after having properly wrapped and
packaged the instrument with care.
2.3 TRANSPORTATION
Before shipping, please keep in mind that:
1) The instrument is very fragile.

2) The instrument is composed of electronic parts.
3) Contact with water and/or any other liquid is to be avoided; please ensure that
the internal plastic protection bag that wraps the instrument is utilized.
4) Transporting and storing temperature(s) must be between -10C +50C.
5) Using the original box, the instrument must always be transported horizontally.
6) Transporting the instrument vertically could result in significant damage as its
center of gravity is quite high.
CHAPTER 3 INTRODUCTION TO THE ATP PROCESSOR

3.1 GENERAL FEATURES
The ATP tissue processor recycles the air utilized to move the reagents to
and from the Retort. Two effective charcoal filters on the external air-intake
reduce the exhaust fumes to acceptable levels.
The control devices (hardware and software) are based on up-to-date

processing control technologies. A 9.5 backlit LCD touch screen display
makes the instrument easy and efficient to use.
Up to 12 different programs can be permanently stored and can be easily

modified. A 13th program is a special REVERSED program that is used to deprocess samples where further procedures are required.
During a run, every step and action is displayed on the screen, such as current
step and function (emptying, filling, etc.), Retort and paraffin heating chamber
temperatures, Retort pressure, completion time and date.
In the event of blackouts, the computer saves all the data necessary to restart
the process exactly where it was interrupted. If the interruption happens during
the paraffin stages, particular precautions are taken to guarantee melting of the
paraffin before any paraffin filling or emptying steps begin.
The ATP has 2 stainless steel baskets of identical dimensions. Each of the
baskets can hold from 125 cassettes (using the appropriate spring organizers)
up to 150 cassettes (without using spring organizers). The processing capacity
therefore varies from 250 cassettes (with organizers) up to 300 cassettes
(without organizers).
Spring separators are recommended when processing medium to large tissue
samples to allow for good fluid exchange. Separators also impede the opening
of the cassettes when processing a small number of samples.
3.2 FRONT VIEW
Tilting color touch

screen
Retort
Floppy
drive
Heated paraffin
chamber
(5 bottles)
Reagent bottle
chamber
Charcoal filter
bottles
10
3.3 REAR PANEL & FLOPPY DRIVE
On the rear panel of the control module there are:
A = On/Off main switch

B = Main power connection module
C = Fuse holder (2 x 10A fuses)
D = Keyboard Connector (for use by service technicians)
E = Remote Alarm socket:
No alarm = pins 1-2 closed, pins 1-3 open
Alarm
= pins 1-2 open, pins 1-3 closed
Please note: The socket at E is a low voltage connection (maximum 48V,

6A) isolated from the rest of the instrument. An Auto Dialer or other external
alarm notification device can be connected here. The device must output less
than 48V, 6A or it will damage the alarm relays.
F = RS-232 serial port
G = Reset button
E
1
2
3
Floppy Drive
The 3.5 floppy drive on the side of the ATP is only used by service technicians to
update the software.
11
CHAPTER 4 REAGENTS
4.1 COMPATIBLE REAGENTS

The following reagents can be utilized in the ATP without any risk of damage:
Water
Formalin
Ethyl alcohol (pure or denatured)
Methyl alcohol
Isopropanol
Butanol
Xylene
Toluene
Paraffin Wax
The following materials are used in the construction of the ATP. Reagents other than those
listed above can be utilized in the instrument if they DO NOT damage the materials listed:
Stainless steel
Teflon
Kynarflex
Viton
Glass
HDPE
Nylon
Tygon
Nickel-plated brass
Rotating valve, Retort and lid

Rotating valve, air pump
Reagent pipes
Membranes
Vapor trap
Paraffin and reagent bottles
Connectors
Air pipes
Air connectors, solenoid valves
The manufacturer is NOT responsible for damages due to the use of reagents NOT listed here.
12
4.2 INCOMPATIBLE REAGENTS

Below is a short list of well-known reagents that CAN DAMAGE the instrument. This list is NOT
complete. Before using reagents not explicitly noted in this manual, please contact TBS technical
service for assistance.
Acetic Acid
Acetone
Amyl acetate
Benzene
Bouins fixatives
Carnoys fluid
Carnoy-Lebrum fluid
Cedarwood oil
Chloroform
Dioxane
Ethyl ether
Flemmings Strong
Gendre fluid
Hellys fluid
Littles alcoholic lead
Olmacher fluid
Osmic acid
Paraformaldeide
Perfix
Petroleum ether
Picric acid
Rossmans fluid
Tetrahydrofuran
Trichloroacetic acid
Trichloroethane
Weigirt lodine
Zambinis PAF
Zenkers
The manufacturer is NOT responsible for damages due to the use of the above stated reagents.
WARNING: DO NOT use Acetone, Benzene or Trichloroethane. We

also advise against the use of fixatives containing mercury salts, acetic
or picric acid as they may corrode the metal components of the
instrument and shorten a components useful life.
13
CHAPTER 5 INSTALLATION AND OPERATIONAL QUALIFICATION

This procedure describes the steps for installation and testing of the basic mechanics of the unit
and should be done on all new units or units returning from factory service.
1. Position the unit on a flat level surface away from heat (radiators, stoves, direct sunlight,
etc.) and moisture (sinks, drains, etc.).
2. Connect the unit to a power source. The main power supply connection must provide
(depending on the model):
110 VAC +/- 10%; 60 Hz; 10A; grounded
220 VAC +/- 10%; 50 Hz; 6A; grounded
In addition to the ATP anti-blackout feature, an uninterruptible power

supply (UPS) can be utilized to provide power for short power outages
and some protection against power fluctuations, line noise and power
spikes. Use of a UPS is highly recommended for sites which
experience brown-outs or other power fluctuations.
The use of adapters to plug in more than one instrument to the same
outlet (power strip) does not provide a reliable power supply. It may
produce overheating and create a potential fire hazard. ECC electrical
instrument directives forbid such practices.
3. Turn the power on.
4. Select the preferred Language on the initial screen.
5. From the Main screen select Service/ Edit Setup. Set the elevation to the appropriate
value for the location of the laboratory. (See Chapter 12 for more details.)
This is particularly important for higher elevation location. Failure to set

the elevation could result in short fill and pressure errors.
6. Choose your Alarm settings.
7. From the Main screen select Service/ Pressure Test. Perform a pressure test to
confirm the operation of the pumps and sensors associated with the pressure system.
8. From the Main screen select Service/ Vacuum Test. Perform a vacuum test to confirm
the operation of the pumps and sensors associated with the vacuum system.
9. From the Main screen select Service/ Retort Heating. Perform a Retort heating test.
14
CHAPTER 6 PREPARATION FOR OPERATION

6.1 FILLING REAGENT BOTTLES
The emptying and subsequent refilling of reusable reagent
bottles must be done in accordance with all safety
regulations for handling flammable and toxic substances.
Use proper ventilation and keep away from open flames
and/or electrical circuits.
1. Place bottles on a flat level surface for filling. All Bottles must be filled to the level indicated
by the markings on the bottle (between the Min and Max marks):
MIN = 2.5 liters, MAX = 2.75 liters.
Failure to fill to the correct level can result in errors and alarms.
2. Inspect the Quick connect O-rings and inspect the internal tubing connection to ensure
there are no cracks or defects, and that the O-rings are clean.
3. Inspect the openings in the Quick connect to make sure there are no clogs or obstructions.
When exchanging or refilling bottles, use the special screw-caps

supplied with the instrument to avoid reagent spillage and fumes.
Replace cracked or damaged caps and O-rings.
4. Secure the cap around the Quick connect, and the solid cap.
DO NOT OVER-TIGHTEN. This can result in cracking of the caps.
If the quick connect couplers leak, the o-rings should be replaced. If

leakage problems persist, contact TBS customer service.
15
6.2 FILLING PARAFFIN BOTTLES

The ATP tissue processor is unique in that the same bottles are used
for paraffin and reagents. Paraffin bottles are placed in a special heating
chamber that maintains a constant temperature of (54-60 C). There
are 5 paraffin bottle stations. The slot labeled as R contains a spare
bottle which can be used for immediate replacement of a contaminated
or dirty bottle.
Follow the steps outlined above for inspection and filling of the Paraffin Bottles.
Bottles must be filled with melted paraffin to the level indicated
(between the Min and Max marks) whereas: MIN = 2.5 liters, MAX =
2.75 liters.
Use of a paraffin dispenser such as the TBS Paraffin Dispenser (Catalog No. PD) makes filling of
bottles simple and convenient.
DO NOT use paraffin that is not completely melted. It can result in a
clogging of the tubing. Attempting to fill the bottles with flakes
frequently results in an over or under fill condition that will cause a
blocking alarm. (See Chapter 7 for alarm listings.)
WARNING: Since the normal paraffin temperature approaches the

level at which a first-degree burn may be possible, we recommend to
always wear protective clothing, gloves and eyewear when handling
bottles of liquid paraffin.
Always cap open paraffin bottles immediately after they are removed from the paraffin heating
chamber.
The paraffin heating chamber door is insulated and must be kept closed except when removing or
replacing bottles.
The ATP Paraffin Wax Purification cycle removes xylene from the paraffin by drawing a vacuum
on the wax bottles. When particulate build-up occurs in the wax, the wax should be replaced.
Paraffin waxes tend to leave residues that, if not removed, may cause
damage to the instrument. Regular paraffin changes are essential for
optimum processor performance.
16
WARNING: Never leave a slot without a bottle; the bottles should

always stay in their slots with their normal paraffin level of 2.5 lt. The
absence of one bottle may cause an incorrect heating of the other
bottles.
As with all tissue processors, good processing quality requires that certain guidelines be followed:
For a large quantity of samples, or very large specimens, the reagents will need to be
replaced more often
Larger specimens require longer times at each station
The use of vacuum (or pressure/vacuum cycles) and reagent heating in each step of the
process improves infiltration.
The daily replacement of the most contaminated reagent, with subsequent scaling of the
remaining reagents, is preferable to the weekly substitution of all reagents. In this way, the
process quality remains constant; while in the latter method process quality varies from a
maximum (with all new reagents) to a minimum (last process before all reagents are replaced).
The ATP Reagent Management System (RMS) will optimize the scaling process because the
RMS eliminates having to move the least contaminated reagents into adjacent slots. The RMS
automatically selects the most contaminated reagent first and the least contaminated reagent last.
For a more detailed explanation, refer to Chapter 9.
6.3 INSTALLING CHARCOAL BOTTLES
1. Remove the Solid Cap
2. Remove the tape covering the vent hole, or punch a hole in the tape, making sure that the
opening is fully open to allow the Charcoal to vent properly.
3. Place the bottles in the 2 right most positions of the Reagent chamber, one on each level.
Make sure the bottles are firmly positioned and properly seated.
17
CHAPTER 7 FIRST RUN PROGRAMMING

7.1 INTRODUCTION
Prior to running the unit for the first time, there are a number of custom settings that should be set
by the Supervisor.
Reagent Names: The list of available reagents contains default identifications for quality
assurance testing. The user can add the names of other reagents to this list that are
appropriate for their laboratory.
Hour & Date
Program Name and Program
7.2 LANGUAGE
Choose the preferred language for the laboratory.
18
7.3 MAIN MENU
This is the Main Menu:
Software Version
Menu Name
Reagent Management System (RMS) Status
Wax Purification Cycle (WPC)

Status
Process Counter
Charcoal Filter Status
Retort Temperature
Wax Oven Temperature
Retort Pressure
19
At the bottom of the screen are buttons used for navigation:

ENTER Data confirmation, process starting, menu selection, etc.
+ Increase of numerical values and other parameters
- Decrease of numerical values and other parameters
Cursor movement through the menus and the program displays
VACUUM RELEASE Vacuum release in the Retort
CLEAN Retort washing (Clean Cycle)
ESC Back to main menu, stop process, etc.
Above the buttons, two message lines display information for the correct use of the instrument.
These messages show the processing status and any actions that must be performed (not
displayed in the image above since no process has been programmed). The indications contained
in these messages must be carefully followed especially during start-up and the execution of
Processing Programs and Clean Cycles.
All process data are displayed in the central part of the screen both during programming and
execution.
7.4 REAGENT LABELS
From the Main Menu, select SETUP to access the Setup Menu.
20
Choose REAGENT LABEL.
Reagent names must be defined before they can be used in a program.

Up to 12 different reagent names can be defined.
Use and arrows to select the position (name) you wish to edit.
Use and arrows to select the letter you wish to change or add on.
Use + and - to access the alphabetical list until the letter you want is displayed.
Use VACUUM RELEASE to select upper-case, lower-case and numbers.
Use ENTER to confirm and save changes.
Use ESC to abort the operation. All the changes will be lost.
Use CLEAN to create blank spaces.
It is not necessary to confirm with ENTER each single name or letter change. After modifying one
or more letters of a name, switch to another name or letter using the arrow keys and then confirm
with ENTER all the changes simultaneously.
7.5 PROGRAM LABEL
Program names must be defined before the program can be defined or edited. Up to
12 different program names can be defined.
21
Use and arrows to select the position (name) you wish to edit.
Use and arrows to select the letter you wish to change or add on.
Use + and - to access the alphabetical list until the letter you want is displayed.
Use VACUUM RELEASE to select upper-case, lower-case and numbers.
Use ENTER to confirm and save changes.
Use ESC to abort the operation. All the changes will be lost.
Use CLEAN to create blank spaces.
It is not necessary to confirm with ENTER each single name or letter change. After modifying one
or more letters of a name, switch to another name or letter using the arrow keys and then confirm
with ENTER all the changes simultaneously.
7.6 HOUR
AND
DATE
Select HOUR and DATE from the Setup Menu.
The Hour and Date Screen:
With the arrow keys, point the cursor at what is to be changed, then increase or decrease its
values with the + and - keys.
Press ENTER to save changes and to go back to the previous menu.
Press ESC to abort changes and to go back to the previous menu.
22
7.7 PURGE REAGENTS LIMITS SETTING
Access these settings via SETUP/RMS. The reagents for the Clean Cycle are managed by the
RMS. It is necessary that the RMS be active in order to receive reminders to substitute the Purge
reagents every 3 to 9 processes. The recommended numbers of processes which can be
performed before the substitution of the Purge reagents are as follows:
Cleaning only the retort: 9 processes or less

Cleaning the retort and the baskets: 5 processes or less
These recommendations are valid for the correct use of the instrument. However, they are
contingent upon the manual removal of as much residual paraffin as possible (from the lid and
retort) before beginning the Clean Cycle.
Please note: if the user desires to replace the Purge reagents before their counter
reaches the preset limit, it will be necessary to reset the counter by the Zero Resetting
Single Counter function.
7.8 CCW (PARAFFIN OVEN) TEMPERATURE SETTING
When the ATP is delivered to your location, the temperature of the CCW (stations 11 to 14) is
preset to the default value of 60C. This default can be modified in the Other Settings /Functions
section of the RMS Setup menu. The allowed temperature range for paraffin in the CCW is from
50C to 60C.
At the start of any process, the default temperature of the CCW is reset to be the same as the
retort temperature set for the paraffin waxes in that process (if they differ). At the end of a process,
the CCW temperature will return to the default value selected in the RMS Setup menu.
It is important to note that if the instrument is completely reset (as explained in

Chapter 7 Alarms) this default CCW temperature will return to 60C.
7.9 CHARCOAL FILTER LIMIT SETTING
The charcoal filters are consumable items administered by the RMS. The percentage indicator
(amount of usage) is visible on the MAIN and SETUP menus (with 0% representing a new filter
and 100% representing an expended filter). When the filters reach or exceed their preset limits, the
RMS will prompt the user to replace them at the same time they are prompted to replace reagents.
23
The charcoal filters should be replaced any time after 60 processes but under no circumstances
more than 120 processes based upon the following factors:
Without use of the WPC, replace every 90 to 120 processes.

With use of the WPC, replace every 60 to 90 processes.
These values are somewhat approximate because they can vary due to:
Environmental factors (humidity, temperature)

The duration of the processes
The application of vacuum (or pressure) on all the processing steps
The frequency of agitation of the reagents
If you are operating the ATP in an area with high relative humidity, if you run long processes
frequently, if you apply pressure and/or vacuum to most or all of the process steps, if you agitate
the reagents in the retort frequently, and especially if several of these factors apply to your usage,
it will be necessary to set your process number limits for the charcoal filters at or below the
minimum values mentioned above.
Important note: If you replace your charcoal filters before the process counter
reaches your preset limit, you must reset the counter with the RMS Single Counter
Reset function on the RMS Setup menu. If this becomes a common practice,
please edit you process limits for the charcoal filters.
7.10 WPC (WAX PURIFICATION CYCLE)
ACTIVATION / DEACTIVATION
Use of the WPC will reduce your consumption of paraffin by approximately 50%. The system is
able to reduce paraffin consumption by removing the preceding reagents that would be introduced
by cross-contamination. This removal is accomplished by a flow of air bubbled through the paraffin
containers. The volatile reagents evaporate due to the heating of the paraffin containers during the
WPC process. The air bubbled through the paraffin carries these reagent vapors through the
charcoal filters where they are trapped. Use of the Paraffin Wax Purification Cycle, while it will
reduce consumption of paraffin waxes, will also shorten the estimated life of the charcoal filters.
The duration of the Paraffin Wax Purification Cycle is about 30 minutes.
If the WPC option is activated, it will begin automatically after each Clean Cycle, but
only when paraffin has been used in the preceding process.
24
It is always possible to manually run a Paraffin Wax Purification Cycle from the RMS Setup Menu,
independent of paraffin usage in the previous process. During the WPC the paraffin waxes are not
transferred from their containers; the retort will stay clean and it will not be necessary to start a
Clean Cycle after the WPC.
During the WPC, the retort lid must always stay closed.
The activation / deactivation of the WPC takes place on the RMS Setup menu. The WPC should
be activated for optimal performance.
Deactivation of the WPC is advisable when:
An excessive consumption of the charcoal filters makes its use less than economical for the
user. This could occur due to a combination of the environmental and usage factors
mentioned above.
The instrument is malfunctioning frequently. Discontinuing use of the WPC lowers the
workload on the ATP and eliminates one possible source of malfunction. This in turn
assists service personnel in diagnosing the instruments problem.
7.10.1 MANUAL START
OF THE
WPC
If for any reasons the WPC has been interrupted or not allowed to start automatically, it can be
started manually by selecting the Start Paraffin Wax Purif. Cycle line on the RMS Setup menu. It
is important that the WPC is started only after running a Clean Cycle following a process.
7.11 RMS ACTIVATION
The RMS (Reagent Management System) can be activated or deactivated prior to running a
program.
See Chapter 9 for details on the use and setup of the RMS.
25
CHAPTER 8 PROGRAMMING PROCESSES

8.1 PROGRAMMING PROCESSES
To define new or edit existing programs, the Program Label must exist, and any
reagents to be included in the program must already be defined.
1. Select EDIT PROGRAMS from the MAIN menu.

2. From the menu on the right side of the screen, use the arrows to select which program you
would like to edit. Once highlighted, press ENTER
The Edit Programs Screen will appear.
Use and arrows to select the process to edit/change.

Use ENTER to start editing the selected process.
Use the ARROW keys to move through the process data: Reagent name, Processing Time,
Temperature, Pressure/Vacuum and Agitation.
When in the field to edit or change, use + and - keys to change values.
Press ENTER to save changes and to go back to the previous menu.
Press ESC to abort the operation and to go back to the previous menu.
26
Reagent
Reagent names are labels prepared in advance. They do not have an influence on the
process. They are listed for the convenience of the user so that you can define which
solutions to use for each step
Time
The time for each step can be set from 0 to 12 hours, in 5 minute intervals. If a time of 0
hours and 0 minutes is set, the step will be ignored even if the other parameters
(temperature and vacuum) are set. The time includes the reagent filling and emptying
process.
Temp
The selectable reagent temperature range is:
The selectable paraffin temperature range is:
Ambient - 45 C
52 - 60 C
P/V
The Pressure/Vacuum P/V field allows you to select the following options:
A = ambient pressure
V = vacuum approx. 700mBar below ambient
P = pressure approx. 250mBar above the ambient pressure
P/V = an alternating cycle with an 8 minute frequency of pressure and vacuum
Varying the pressure in the Retort facilitates reagent infiltration into tissue specimens. The
following P/V settings are recommended:
Biopsies = vacuum or ambient pressure
Samples of normal size = vacuum in every station
Mix of biopsies and normal samples = vacuum in every station
Large samples = cycles of pressure and vacuum in every station
It is never recommended to simultaneously process samples of vastly different sizes.
27
Agit
The reagent agitation in the ATP is accomplished by bubbling air up from the bottom of
the Retort. Select from the following frequencies:
0 = no agitation
1 = an agitation every 30 minutes
Each agitation cycle lasts 10 seconds.
Actual dye dilution studies indicate better reagent mixing throughout the retort (from top to bottom)
with air bubble agitation than conventional magnetic stirring.
8.2 PROCESS # 13: REVERSED

REVERSED possesses all the characteristics of the other programs except:
It begins at the last non-zero time on the steps list and moves backwards (REVERSED).
It is not possible to insert a delay on the first step.
The operator cannot modify its name.
Its position in the list of the processes is always #13.
Except as specified above, it is identical to the other processes, therefore:
It increments the RMS counter by one process.

All RMS rules are respected.
At the end of the process, user will be prompted to run a Clean Cycle.
At the end of the Clean Cycle, if during the last process at least one paraffin used, the WPC
will automatically be started.
At the end of the WPC, the Reagent Management System will be started.
There are no particular restrictions to the use of this process, however be aware that the reagent
contamination will also be reversed.
Under normal conditions this process would rarely be selected, as it serves primarily to unprocess
samples of poor quality, therefore it will not substantially influence reagent concentrations.
28
CHAPTER 9 REAGENT MANAGEMENT SYSTEM (RMS)

9.1 INTRODUCTION
The ATP RMS is a system to punctually and exactly manage all reagents to enhance processing
consistency and improve sample quality.
The use of the Reagent Management System provides quality assurance documentation of
reagent bottle status. This results in substantial time savings and reduces the possibility of errors
when multiple operators use the instrument.
When activated, following the Clean Cycle (and the Wax Purification Cycle, if activated), the
system will automatically prompt the user to replace the reagents that have reached their userdefined pre-determined processing limit.
Access the RMS Setup Menu by choosing SETUP/RMS.
9.2 CHOOSING
THE
RMS MODE
This will allow you to select either of the following two functions:
29
Single Bottle mode: In Single Bottle mode, each bottle is managed as a separate
entity, therefore, each bottle will have a defined limit usage and will be defined by the
specific type of reagent it contains. In this mode, the number that identifies the physical
position of the bottle in the processor always corresponds with the program step number.
It is important to understand this operation of the processor and of the RMS. Unlike the
Group Mode, in the Single Bottle Mode the program step # and the identification # of the
bottle are the same, plus, the reagent is identified by its specific name (i.e. 70% alcohol,
xylene, etc.).
Group mode: The intent of the Group mode is to automate the reagent management
process that is performed manually in many laboratories. The physical shifting of the
bottles by the operator is not necessary because the ATP automatically changes the
order of use of the bottles in relation to the prior usage of the reagent. In the Group
mode, reagent bottles are managed as a group based on the type of reagent contained
in the bottles.
The RMS automatically keeps track of the number of processes performed and the
total number of cassettes processed. Changing the RMS mode will reset the counter
to zero, therefore, it is recommended that all reagents are replaced prior to
changing the RMS mode.
30
9.2.1 RMS SINGLE MODE DESCRIPTION
AND
EXAMPLE
The RMS Instructional Screen indicates what action to take.
As explained in previous sections, at the end of each process and after the completion of the WPC
(when active), the Reagent Management System will activate a screen like the one shown above.
This screen provides written instructions and a graphical representation of the ATP, showing
which reagent bottles to replace and the specific reagent to use.
If, for some reason, it is necessary to postpone the substitution of reagents after a process, it is
possible to press the ESC key, skip this session of the RMS and postpone it until the end of the
next process.
Please note: The process limit counters are not reset by this action. At the end of the
next process, the RMS will prompt the user to replace the reagents that expired at the
end of the previous processes plus those whose limits have been reached after this
most recent run.
If the RMS is skipped at the end of a process using the ESC key as explained above, scheduled
reagent replacement can be performed without waiting to complete another process. By choosing
to enter the RMS menu from the Main Menu, you will automatically be prompted to press enter to
start RMS operations.
Conversely, if the RMS has been regularly executed, any request for execution from the Main
Menu will be denied.
31
9.2.2 RMS GROUP MODE DESCRIPTION
AND
EXAMPLE
This mode facilitates maintenance, management and quality assurance by:
Managing the bottles by user defined classification of the reagent.

Recording the usage of each solution by number of runs and/or cassettes.
Reminding to replace reagents that have reached their defined limits.
Automatically selecting the proper order depending on the usage of each reagent within the
specified group.
In the group mode, the specific reagent name is not used to identify the contents of the bottle, but,
it is classified as part of a group of similar reagents. The position number of a reagent bottle does
not correspond to the program step number with the exception of the following:
The first time the processor is used in Group mode

Each time the counters are reset to zero (RMS resetting)
Intermittently, when the relationship between the number of bottles in the group and their
individual limits is such that the position number is the same as the bottle number (see the
sequencing example that follows for more detail)
In summary, in order to properly utilize the Group Mode function:

1.
2.
3.
4.
Activate the RMS in Group mode.

Define groups of reagents by their function or contents.
Define the limits, in terms of processes and/or samples for each group.
Replace the reagents as indicated.
Example Group Mode Sequence
High grade dehydrants are placed in bottles 6, 7 and 8.

The limits of this group are fixed as 1 process.
The sequence of use would be the following:
STEP
PROCESS # 1
6
7
8
PROCESS # 2
6
7
8
USAGE
Bottle 6 (fresh reagent)

Prompt to replace bottle 6
Bottle 7
Bottle 8
32
PROCESS # 3
6
7
8
PROCESS # 4
6
7
8
Prompt to replace bottle

Bottle 8
Bottle 6
Bottle 6
Bottle 7
Since the limit is set at 1 process, the user will be prompted to change the most contaminated
bottle in the set, the first bottle, following the first run. The second bottle is then accessed first on
the next run, and would become the next bottle to be replaced.
In this example, for process #4, the reagent group sequence will correspond again to the physical
position of the bottles and to the step of the program. For the paraffin group (that is composed of 4
bottles), the frequency of a return to the physical order is 4 processes.
Understand that when the RMS is operating in Group mode, neither the physical position of the
bottles nor the step of the program when selecting a reagent will be relative to the cumulative
usage of the reagent. The instrument will provide instruction for the reagent substitution and it will
be essential to follow the instructions exactly.
The processing results will be the ultimate indicator as to whether the RMS settings are ideal. To
correct inadequate infiltration replace all the reagents, use the counters reset functions to set the
group counters to zero and decrease the process/cassette number limits. Alternately, excellent
results may indicate that the limits could be increased slightly, extending the life of the reagents.
33
The RMS Instructional Screen indicates what action to take.
Similar to Single Bottle mode, at the end of a process the RMS will prompt the user with graphics
and text to replace a reagent bottle from one or more groups. This task may be postponed to a
later time by pressing the ESC key in the same manner mentioned above, and, may be executed
at the end of the next process or accessed from the Main Menu.
When there are multiple concentrations belonging to a group such as the DEHYD
groups, the highest concentration solution should be utilized to replace the reagent in
the bottle.
34
9.3 RMS PROGRAMMING REAGENTS DEFINITION
Defining reagents allows the operator to identify the type of reagent that is utilized in any single
bottle. The labels utilized are the same as those assigned in the process programming section
(Chapter4) and that are prepared/modified from the SETUP Menu with the function Reagent
Label.
Please note: When using the RMS, the assignment of a reagent name to a bottle,
does not automatically update the reagent names in the process programs.
The way the reagents are defined depends on whether the RMS is operating in SINGLE mode or
GROUP mode.
Single Mode
The content of each bottle needs to be defined by highlighting the bottle position and using the +
and keys to scroll to the correct reagent name. Once the correct name is indicated, use the
arrow key to proceed to the definition of the next reagent bottle.
Group Mode
The contents of each bottle are classified according to the type of reagent, not the specific reagent.
Thus each position needs to be defined as one of the following classes of reagents.
FIXATIVE: Formalin or other similar functioning reagents

DEHYD L.G.: Low Grade Dehydrant such as Alcohol up to 70%
DEHYD H.G.: High Grade Dehydrant such as Alcohol >70%
CLEARING: Xylene or other similar functioning reagents
WAX: Paraffin wax
UNDEFINED
The content of each bottle needs to be defined by highlighting the bottle position and using the +
and keys to scroll to the correct group name. Once the correct name is indicated, use the arrow
key to proceed to the definition of the next reagent bottle.
The definition of the groups must respect the following rules:
The groups must be sequenced in relation to their actual use in the processing procedure
(i.e., fixative must precede dehydrant, low grade dehydrant must precede those of high
grade, etc.).
35
Between one group and another there could be bottles of undefined content that therefore
are not managed by the automation of the RMS. This, however, is unadvisable because it
is inconsistent with the goals of the Histology process.
A group could consist of just one bottle (a typical example is the group of fixatives).
For the dehydrants there are two separate groups: low and high grade (low grade being
alcohols with concentrations less than 70%). If the user desires though, it is possible to
have only one group, choosing either low or high grade to represent it, and having the RMS
manage your alcohols as one large group.
Bottle positions 11-14 are fixed as the paraffin group. If you wish to use the RMS to
manage your reagents but do not wish to have the RMS manage the paraffin waxes for you,
it is necessary to set the paraffin process number/cassette number limits to zero.
9.4 RMS PROGRAMMING LIMITS DEFINITION
The definition of the limits determines the frequency of reagent substitution. It is possible to assign
each bottle a limit based on the number of processes performed and/or a limit based on the
number of cassettes processed.
When one of the two limits is reached or exceeded, the RMS will require the substitution of the
reagent for that specific bottle. Again, it is sufficient that only one of the two limits is reached. It is
possible, however, to use just one of the two limits by setting the other to zero. If both limits are
set to zero, the RMS will not prompt the user to replace the reagent in that bottle.
Single Mode
The limits of each bottle need to be defined by highlighting the bottle position and using the + and
keys to scroll to the correct limit for both CASSETTE number and PROCESS number. Once the
correct limit is shown, use the arrow key to proceed to the definition of the next reagent bottle.
36
Group Mode
The limits for each reagent type needs to be defined by highlighting the bottle position and using
the + and keys to scroll to the correct limit for both CASSETTE number and PROCESS number.
Once the correct limit is shown, use the arrow key to proceed to the definition of the next reagent
type.
When defining the process number/cassette number limits for groups of reagents, the same factors
that were considered when assigning single bottle limits will be considered here. The difference is
that when the preset limit for the group is reached or exceeded, the RMS will require the
substitution of the bottle whose reagent is the most polluted (the bottle in the group that, in the
previous processes, was used first).
9.5 RMS COUNTERS TOTAL RESET
The Counters Total Reset selection allows the user to set to zero the number of processes
performed and the number of cassettes processed. This function may be used when it is
determined that it is necessary to replace/renew the reagents of all bottles and, in effect, restart the
RMS. The complete zero resetting does not include the counters for the charcoal filters or the
bottles of the Purge reagents.
Please note: Resetting the counters to zero occurs automatically
when the following functions are selected:
Changing the RMS mode (between Single and Group mode)
Each time the RMS is activated or deactivated
When the definition and/or limits of the groups are modified
Please note: Resetting the counters to zero does not occur
automatically when modifications to the definition of the reagents or of
the limits in Single Bottle mode are made.
RMS Single Counter Reset

The Single Counter Reset selection allows the user to zero the counters of individual bottles. This
function is useful if the user decides to replace/renew a reagent before the preset limit of a
particular bottle is reached.
Please note: The Single Counter Reset only allows the user to
change individual bottle counters if the RMS is in Single Bottle mode.
Please note: The Single Counter Reset is also the only function that
allows the operator to reset the counters of the charcoal filters and the
Purge reagents.
37
Please note: The counters of the charcoal filters and Purge reagents
are not reset by the Counters Total Reset function or a change from
Single Bottle mode to Group mode. Whenever the user replaces the
Purge reagents or the charcoal filters without being prompted by the
RMS, it will be necessary to utilize the Single Counter Reset.
9.6 RMS ON / OFF

This function will turn the RMS on or off. Changing the RMS mode will reset the counter to zero.
Again, it is recommended that all reagents be replaced prior to changing the RMS mode.
9.7 PRINTING MENU

The Printing Menu:
From the Printing Menu you may print the following information:
Alarm Archive: Report of the last 100 alarms

Setup Parameters: Various Instrument Setup Parameters
RMS Situation: Status of the RMS
RMS Setup: RMS settings
38
It is always advisable to print the RMS Setup and general Setup Parameters after any meaningful
changes have been made to their values.
A printout of the RMS status can be useful if there is some question about the cycle of reagent
substitution or if there is some other reason to refresh or exchange some reagents and reset their
process/cassette number counters.
Finally, although the user is asked at the end of each process if they would like to print a report of
the process just performed, it can also be printed from this menu.
If you attempt to print with no printer attached, the system will lock up. If this happens,
power cycle the unit and then clear the resulting Blackout Alarm.
39
CHAPTER 10 NORMAL OPERATION

10.1 LOADING SAMPLES
INTO THE
BASKET
The standard sample basket has 4 metal dividers and springs installed to allow cassettes to be
organized and to prevent cassettes from opening during a run. The image below shows a number
of cassettes loaded using the springs. If you prefer not to use the dividers or springs, they can
easily be removed.
It is always a good idea to evenly space

the cassettes in the basket to allow for
maximal fluid flow around the cassettes
for better penetration of reagents and
infiltration of wax.
This can easily be achieved using the

spring dividers.
For maximal capacity, the cassettes can

be stacked together and held in place
with the spring, if desired. This is
shown on the top left side of the basket.
Place the lid securely on the basket, load the basket into the carrier and position the carrier into the
retort.
10.2 CHECKS
AND
PROCEDURES
BEFORE
OPERATING
Check paraffin and reagent bottle levels on level surface (between min and max).
Check bottle caps for cracks, and secure them.
Inspect O-Rings.
Check and, if necessary, clean the Retort.
Place the samples into the Retort.
Close and latch the Retort lid.
Select a program.
Edit date and time of process-end (a delay function on first step if desired).
40
10.3 SELECTING
PROGRAM
Select START PROCESS from the MAIN menu.

The screen now should look the same as the Edit Program screen.
Use the and arrows to select the Program that is to be utilized.
Press ENTER to start the Program.
10.4 SETTING
THE
PROGRAM COMPLETION TIME/DATE
The Start Process Screen:
Two lines are displayed above the icons.

The first line displays the process duration plus the possible presence and duration of any delay in
DAYS, HOURS and MINUTES.
The second line will display the time, as determined by the computer, that the process will be
completed.
Please note: The program cannot begin with this screen open; but, the
delay will count down while this screen is open. Once the delay reaches
zero, the Program End time will begin to increase until you begin the run.
41
Setting Delay
Use the and arrows to choose one of the fields which indicate hours, minutes and day of the
week of the program completion.
Use the + and - keys to change the time and date that the program is to end. The computer will
automatically update the total processing time, the date of completion and the fields that indicate
the amount of the delay expressed in days, hours and minutes. The maximum delay is 7 days.
It is not possible to set an hour or a day of the week less than the starting time added to the
duration of the process. As noted above, any time spent in this menu will decrement the delay (if
any) or increment the end time if the delay is zero, until you close the menu.
Any delay programmed will extend the length of time specimens remain in the first reagent.
Programming Completion Times
A convenient feature of the ATP is that it will automatically remember when a program is to be
completed. The computer stores the hour and/or day you have chosen for the process to be
completed. The next time you choose the same program, the computer will indicate the same hour
and /or day of completion. Any delay necessary to end the process at the predetermined hour
and/or day is automatically calculated and displayed in the appropriate display field.
To reset the delay, press CLEAN. The delay will be cancelled and the delay memory will be
erased. The next time the program is started, there will be no delay.
In addition to any special programs, 2 STANDARD programs are recommended:

o Daily - The first time you start the Daily program, set only the time of completion
(e.g.: 7.30am). After that, any time you select the Daily program it will, by default,
propose to end automatically the following day at 7.30am.
o Weekend - The first time you start the Weekend program, set the completion time
and day of the week (e.g.: 7.30am of Monday). The Weekend program will end
automatically on the following Monday at 7.30, again, unless you press the CLEAN
button and then set a new delay and end time.
Completion time cannot be set less than current time plus the time to complete process.
For example, if you start a 12-hour program at 9:00pm and desire an end time of 7:30am,
there is not enough time to complete the process without cutting its time short. The
computer will, therefore, automatically adjust the Process End time so that the 12-hour
processing schedule is maintained, in this case, by finishing at 9.00am the following
morning.
42
It is highly recommended that the user verify the time, day and date
of process completion before starting a program. If it is set
incorrectly, the users only options are to wait for process
completion or to remove partially processed tissues from the retort,
reprogram and begin a new process. Processing programs cannot
be edited once they are begun.
Midweek holidays
There are two convenient solutions for handling midweek holidays:
Create a third standard program, named for example HOLIDAYS, where you can set a
different completion time to meet the particulars of your schedule.
Use the Daily setting, but be certain to reset the completion time/date before utilizing the
program again.
Starting from step other than the first
Use the and arrows. Note that the bar will move from the first step to every valid step (every
step is valid if its value is greater than 0). It is possible to select a starting step different from the
first step in a particular program.
This feature could be used, for example, on partially processed tissue samples to restart
the processing where it was interrupted.
Runtime Test Aborting Run
It is possible to practice setting program parameters, and even to launch a program, because
during the first phase of RUNTIME TEST, you can stop the program simply by pressing the ESC
key, as the instrument has not yet filled the Retort. Be certain to re-enter the desired values before
starting any programs.
43
10.5 STARTING
PROCEDURE
After the date and time to complete a program has been entered, the program can be started by
pressing ENTER.
Use ESC to abort the operation and to go back to the main menu without starting the process.
Process execution:
When the program is started, the computer will run a RUNTIME TEST on the main functions of the
instrument. This test doesnt involve the filling of the Retort and can be stopped at any time by
simply pressing the ESC key.
If an error condition is detected during the self-testing process, the test will be terminated
automatically and a code will appear on the screen.
If the test is successful, the program will start and the program information will appear on the
screen.
The current step is highlighted and the time will count down until the step is complete.
44
Any alarms will appear in the window that displayed the program names in the sequence
that the alarms occurred.
The first line in the message area will show the name of the current program and the
scheduled date and time of completion.
The second line will indicate the step that the processor is performing (e.g.: filling or
emptying reagent, positioning the rotating valve, etc.).
The window at the bottom right displays:

Retort
Retort temperature, in C
Oven
Paraffin chamber temperature, in C
Press
Retort pressure, in millibars
Once a program is started, it requires no intervention.

During the running of a program, it is NOT possible to access other functions or edit the program.
10.6 INTERRUPTING
THE
PROCESS
A process can only be aborted when the second message line displays Processing.
The interruption of a program is only possible when the reagent is in stand-by in the Retort and not
during the other steps (filling/emptying, positioning the rotating valve, etc.).
To abort a process, press ESC and, within 3 seconds, ENTER.
The retort will then be emptied and the samples can be removed. If a paraffin step has already
been executed, the program calls for the Purge of the retort. It then returns to the main menu,
where it is possible to start another program.
The processor does not ask for the Clean Cycle when a program is aborted before using paraffin.
It will be necessary to press CLEAN to start the Clean Cycle or manually clean the Retort before
starting another run.
45
10.7 OPENING
THE
RETORT DURING
PROGRAM
There are some steps in which the retort CANNOT be opened and others in which the retort MUST
NOT be opened.
While a program is running, NEVER open the retort lid in a step other than
Processing. Alarms may occur that would make the computer control system
abort the process. Potentially toxic or flammable substances may exit from the
retort.
The retort can be opened when the second message line displays Processing. Please take the
following precautions:
Close the lid as soon as possible.
Immediately close the lid if the Processing message changes.

Respect all the safety rules about the precautions to take when handling toxic or
flammable substances.
If the system is processing during a vacuum step, it will be necessary to press the key
VACUUM RELEASE before opening the retort. The second message line will then
display Normalizing pressure in the retort, and, after 10 seconds, the retort can be
opened. Within one minute the processor will again try to create a vacuum if the step
requires this operation. If the Retort is not closed within this time, a non-blocking alarm
will occur (VACUUM TIME-OUT) and for the remaining time of the step the processor
will NOT try again to create a vacuum. Processing quality could be compromised.
If the opening of the retort is difficult, DO NOT force it. Instead, press the VACUUM
RELEASE key and try again. This may also be necessary when the closed retort is
passing from a hot to a cold state (e.g., immediately after a process) as this creates
an internal vacuum that makes it difficult or impossible to open.
10.8 PROCESS COMPLETION
At the end of a program, the instrument leaves the last reagent in the Retort to prevent the
samples from drying out. Press ENTER to empty the retort.
After the Retort empties, open the retort and take out the samples (if necessary, press the
VACUUM RELEASE key).
46
After removing the samples a message will appear asking the operator to authorize printing of the
process report (if the printer is not present, or if a process report is not desired, it will be sufficient
to press the ESC key to skip this operation).
If the program included paraffin steps, the Clean Cycle must be run.
10.9 CLEANING
THE
RETORT (CLEAN CYCLE)
Before starting a Clean Cycle:
Empty Retort of all samples

Wipe excess wax from the retort and lid
Wipe excess wax from the lid gasket
Verify reagents levels in Purge bottles P1 & P2
The Clean Cycle is necessary to remove paraffin residue from the Retort. It is always necessary to
do so if paraffin was used during the last step.
It is preferable to wash the retort even if paraffin has not been used.
The removal of any paraffin residue before the start of the Clean Cycle program is useful to extend
the life of the Clean Cycle reagents. Scraping should be done with extreme care. Avoid the use of
sharp tools that can damage the Retort surface. A rubber spatula works well. Even if the Retort is
maintained at the temperature of the last paraffin until the execution of the Clean Cycle, it is
preferable to run the Clean Cycle immediately after the process.
Press CLEAN to start the Clean Cycle at any time except when a program is underway. The
screen will display data about the Clean Cycle steps.
These steps have been set and optimized by the manufacturer and can NOT be changed.
Press ENTER to start the Clean Cycle. As in any program, the Clean Cycle can be aborted by
pressing ESC and then ENTER within 3 seconds.
47
Clean Cycle procedure:
The bottles containing the Clean Cycle reagents are labeled with P1 and P2 and must contain:
(the first from the left, top row) (P1) xylene (or substitutes), the second (P2) 80% or 100% alcohol.
The Clean Cycle reagent (if not managed by the RMS) must be changed
at least every 5 completed paraffin processes.
Take care not to leave fabric or paper residue (normally used in cleaning
procedures) as the material can prevent the complete closing of the main
valve membrane with subsequent damage or alarms. Pieces of fabric
can damage the rotating valve, causing reagent leaks and cross-over
contamination between reagents.
The last Purge step is the drying of the Retort of any alcohol residue. This step is not critical and
can be stopped at any time without damaging the instrument.
48
CHAPTER 11 ALARMS
11.1 MANAGING ALARMS
There are two types of alarms: Non-Blocking Alarms

Alarms 1 through 9 are NON-BLOCKING alarms or WARNINGS that do not interrupt the
program. The event that caused the alarm is not critical for completion of the process, and any
program stoppage could produce worse results than continuing.
With NON-BLOCKING alarms, the program will continue and the screen will display the alarm
number and a brief explanation of the alarm. In this situation, it is NOT desirable to interrupt the
process. Upon completion, the cause of the alarm can be determined and addressed by the
operator.
Procedures listed below for each type of alarm should be followed to correct the cause of the
problem. If following all of the recommended steps to stop the alarm from reoccurring does not
help and/or if the problem persists for several processes, the TBS service department should be
contacted.
Blocking Alarms
Alarms 11 through 53 are blocking alarms. These are true alarms and do interrupt process, as
the cause of the alarm makes it impossible to proceed. Continuing the program could cause more
serious damage than stopping it.
In the event of a BLOCKING alarm, follow the instructions displayed on the screen:
1)
2)
3)
4)
Stop the acoustic alarm.

Try to empty the retort.
Remove the samples.
Reset the instrument.
After the reset, the instrument is again ready to run a program. The alarm could have been simply
an error in closing the retort lid or something else easy to resolve. Before proceeding, please
check the following:
Remove all samples.
Check that the retort does not contain any reagent residuals.
Check reagent and paraffin bottle levels.
Check that the bottles are correctly placed in their housing slots.
Check that the paraffin waxes are melted and, if possible, verify their temperature.
Check and clean the retort lid gasket.
Execute a Clean Cycle to verify the functioning of the instrument.
When uncertain as to the cause of the failure, have a qualified electrician check the line voltage for
transient and/or drop-outs at the receptacle. If the alarm persists, call TBS technical
assistance and do not use the instrument.
49
11.2 NON-BLOCKING ALARMS

1 - BLACK OUT
It indicates that there has been an interruption of power to the instrument. This can occur due to a
loss of power in the users laboratory or merely due to switching the instrument off and then back
on. It does not necessarily indicate that there has been a black out at your location. If the ATP
has lost power during a process, its automatic power outage protection will allow it to carry on from
where it had been interrupted. How it will do so depends upon what station was active when the
power outage occurred, according to the following:
a) Steps 1 to 10 (reagents): No special action, the black-out time is considered processing time
and the ATP continues as if nothing had happened.
b) Steps 11 - 12 (paraffin): Processing will pause (for approximately as much time as the unit was
without power) for the Retort and paraffin containers to return to the proper temperature. The
blackout time and the related heating time are NOT considered processing time.
Cause Loss of power in the lab, a blown instrument fuse, or merely switching the unit off or
jarring the power cord out of the wall or out of the instrument.
Solution If the power supply doesnt return, check the electric network, plug and cable. If
necessary, replace the instrument fuses as described in Chapter 14. The alarm will be
displayed on the screen until the cause is removed. If the instrument is found working (after a
black-out), but, this alarm appears too frequently (e.g. more than once in a month), it would be
prudent to have the unit connected to its own power supply (separate circuit and breaker). As a
separate issue, uninterruptible power supplies (UPSes) may be utilized to protect against power
fluctuations and spikes; but, even larger UPSes will provide only a few minutes of electricity for
instruments like the ATP that consume a large amount of current. CAUTION: All laboratory
tissue processors rely on the fact that power outages at hospitals, clinics and industrial facilities
will be of a short duration (less than one entire process step), as they should have adequate
backup generators, etc. No tissue processor can protect samples totally from long delays,
especially in dehydrant steps. If you anticipate an extended power outage, take steps to
protect your tissue samples or the processing quality could be compromised.
2 - RETORT T LOW
At the end of a step, (after the drain but before the start of the next step) the retort temperature is
not at the value required by the program. This check is done only at the end of a stand-by step
because it is not possible to know how much time is necessary for the reagent to reach the correct
temperature. The time is strictly related to other parameters, such as: required temperature,
ambient temperature, type and quantity of reagent, quantity of samples in the retort, etc.
50
This is a non-blocking alarm because reagent heating is not critical for obtaining good results. If
the alarm involves the Retort heating system, the instrument will possibly be blocked during the
first paraffin step. The process is not ended but the samples will be left in paraffin with little or no
damage as compared to being in other reagents for a long period of time.
This alarm may frequently appear during very short steps, in this case is not a problem because it
is normal that the retort temperature will not reach the set point temperature during very short
steps.
Cause Incorrect working or damage of: temperature sensor, heating resistance, safety
thermostat.
Solution No intervention by the user is possible. If the problem persists, contact TBS for
technical assistance.
3 - SHORT DRAIN
The duration of the drain was too short.
Cause Insufficient level of reagent in the indicated bottle.
Solution Verify reagent levels on level surface, not mounted in unit. If this occurs during
the alcohol step of the Clean Cycle, reduce the percentage of alcohol from 100% to 80%,
but maintain the volume at the normal level.
4 - P/V TIMEOUT
Occurs if the instrument is unable to create processing pressure or vacuum in the Retort at the
scheduled time. The processor will not try to create pressure or vacuum in that step and will go on
with the program. During the next step, if scheduled, it will try again.
Cause Pump failure, lid gasket leakage.
Solution Check and clean the lid gasket.
5 - FULL FILL P
6 - FULL FILL S
Excessive level of reagent in the Retort during the fill; the overfill is recognized (by the sensor S
or by the pressure gradient P) and the filling is stopped before it can produce a failure. The
process can continue without problems.
Cause Excessive level of reagent in the bottle.
Solution Verify reagent levels.
51
7 - FILE NOT FOUND

A file has not been found or an incomplete file has been found in the computer memory; the
recovery systems will replace the lost or incomplete file from a backup copy.
Cause The buffer battery of the CPU memory is exhausted.
Solution No intervention by the user is possible. If the problem persists, contact TBS
technical service.
8 - LONG DRAIN
The time required to drain the retort was excessive.
Cause Insufficient pressure in the retort due to: inefficient air pump, an air leak in the
pneumatic circuit or an air leak from the retort lid gasket.
Solution Check the retort lid gasket; contact the TBS service department for other
causes.
9 - T WAX LOW
During the process but before the first paraffin (step 11), the temperature of the paraffin chamber
has dropped too low; the process will continue until the last reagent. If during the draining of the
last reagent the problem persists, the process will be stopped. (See also Alarm 20.)
Cause The thermo-regulation system of the paraffin chamber is out of order.
Solution No intervention by the user is possible. If the problem persists, contact the
TBS service department for technical assistance
11.3 BLOCKING ALARMS
11 - FILL NO VACUUM
The instrument was unable to produce a vacuum in the Retort while attempting to fill from a
reagent or paraffin station.
Cause During the filling stage, a serious leak in the pneumatic or hydraulic circuit
occurred; a failed pump; an almost empty reagent bottle; or incomplete closure of the retort
lid.
Solution Check the bottle reagent level and quick connector o-rings as well as the Retort
lid gasket. If the problem persists, contact the TBS service department for technical
assistance.
12 - FILL TIME OUT
The filling from a reagent or paraffin bottle has not occurred in the allotted time.
Cause See Alarm 11.
Solution See Alarm 11.
52
13 - OVER FILLS
An overfill of reagent or paraffin has occurred in the Retort (detected by the overfill sensor).
Cause Incomplete emptying of previous reagent due to a solenoid valve failure or a
paraffin temperature problem.
TBS service department for technical assistance.
14 - OVER T RETORT
During a paraffin step the Retort temperature went over the allowed limit (not to be confused with
alarm #20).
Cause Thermostat system damage.
Solution No intervention by the user is possible. If the problem persists, contact the TBS
service department for technical assistance.
15 - TSC OUT
Malfunction of the retort temperature sensor.
Cause TSC sensor failure.
Solution No intervention by the user is possible. If the problem persists, contact the TBS
service department for technical assistance.
16 - SHORT FILL
A fill is completed before the minimum allotted time. The retort will drain and the process will
proceed to the next step. If the problem persists, the process will be stopped.
Cause The reagent bottle quick connector is not fully inserted into the female receptacle;
inefficient air pump.
Solution Check reagent bottle position and fluid levels. If it happens on the alcohol stage
of the Clean Cycle, reduce the percentage of the alcohol to 80%. If the problem persists,
contact the TBS service department for technical assistance
17 - VR OUT
The rotary valve is out of position.
Cause The Rotary Valve motor has failed or the Rotary Valve position sensor has failed.
18 - FILE NOT FOUND
An essential computer file is damaged or cannot be found and the error recovery system failed
trying to correct the problem.
Cause Computer malfunction, back-up battery failure.
53
19 - RETORT T LOW
The retort temperature was too low during the drain following a paraffin step, or the drain cannot
be completed. The process will be stopped to avoid other problems. (See also Alarm 2.)
Cause Retort heating system failure.
20 - WAX T LOW
During the fill of a paraffin, the temperature of the retort was too low. The process will be halted
because the fill cannot be guaranteed.
Cause See Alarm 14.
21 - OVER T WAX
The temperature of the paraffin chamber has exceeded the set point.
Cause Temperature system failure.
22 - TSW OUT
The el
Cause The sensor has malfunctioned.
23 - PRESSURE N.R.
During the first phase of a drain, the pressure in the retort has not reached the correct value. The
process will be stopped.
Cause The air pump or other pneumatic circuit parts have malfunctioned.
TBS service department for technical assistance.
24 - DRAIN NO PRESS
During the second phase of a drain, the pressure in the retort has not reached the correct value.
The process will be stopped.
Cause See Alarm 23.
54
25 - DRAIN TIME OUT

The drain has gone over the maximum time allowed. The process will be stopped.
Cause See Alarm 23.
26 - RET. NOT EMPTY
The user has attempted to begin a process with the retort either not empty or without having run a
Clean Cycle following the previous process.
Cause Due to another alarm or due to an interrupted process, the retort is not empty.
Also caused by neglecting to purge after a process.
Solution Clean the retort, if it is not empty, drain it manually. At the Service Menu
perform the Alarm Reset and the Flag Reset. Return to the Main Menu and start the
Clean Cycle using the CLEAN key.
27 - OVER FILL P
An overfill of reagent or paraffin has occurred in the Retort. (Alarm sensed by fast pressure
gradient during fill.)
Cause Complete emptying of the previous reagent failed.
Solution No intervention by the user is possible. If the problem persists, contact our
technical assistance
30 - COM ERROR [FRAME]
A communication error between the CPU and serial board.
Cause A component or connection in the computer has failed.
Solution Contact the TBS service department for assistance.
31 - COM ERROR [PARITY]
See Alarm 30.
Cause See Alarm 30.
32 - COM ERROR [OVERRUN]
See Alarm 30.
Cause See Alarm 30.
55
33 - COM ERROR [TIMEOUT]

See Alarm 30.
Cause See Alarm 30.
39 - COM ERROR [LINE OR PORT DOWN]
See Alarm 30.
Cause See Alarm 30.
40 - ELODEV LACK
A failure of the touch screen. The display will still be functional.
Cause A failure has occurred in the Touch Screen membrane circuit, power supply,
connectors, CPU board, or Disk-on-Chip.
Solution A standard computer keyboard may be connected to the 5 pin din connector
keyboard connector next to the remote alarm connector on the upper rear of the ATP. All
the functions of the ATP can be operated from the keyboard if the display screen is
working, but not the touch screen membrane. Contact the TBS service department for
assistance.
50 - OVER PRESSURE
The purpose of this alarm is to exclude the clogging of a pneumatic line, which greatly reduces the
volume of the overall plumbing system, from the causes of alarms # 2, 4, 5, 8, 11, 12, 23, 24, 25,
27.
Cause A blockage in a pneumatic system of the ATP prior to the Retort. The problem
may be a bad connection or a faulty signal from the pressure transducer. The pressure
transducer may have failed.
Solution Check: the pneumatic plumbing system between the pump and the Retort for
blockages. Contact the TBS service department for assistance.
51 - OVER VACUUM
See Alarm 50.
Cause See Alarm 50.
52 PS BREAK HIGH
Pressure Transducer problem.
Cause See Alarm 50.
56
53 PS BREAK LOW
Pressure Transducer problem.
Cause See Alarm 50.
11.4 ALARMS HISTORICAL ARCHIVE
The alarms historical archive is under the SETUP menu and consists of a list of the last 100 alarm
occurrences. This feature assists a service technician to identify the problem, its frequency, the
step in which it occurred and other useful information to help solve the problem.
Alarms are displayed from top to bottom on the screen, starting with the most recent problem. Use
arrow to display older alarms. When it reaches the end of the alarm list, the system goes back to
MAIN menu. Use ESC to go back to MAIN menu.
11.5 INSTRUMENT RESET
In the event the software has locked up and the instrument ceases operation, the software can be
reset by the following procedure:
On the rear of the instrument there is a green reset button. Its function is to reset the software
under the following conditions:
If, for any reason, the user is unable to reset an alarm from the keyboard
If a user faces some other unexpected situation that cannot be resolved by any other means
To reset that part of the instruments memory that holds variables pertaining to the state and
content of the retort, possible steps in process, possible actions in process and/or alarms in
process
57
The procedure for using the reset button is as follows:
Switch off the instrument (the switch is located where the power cord enters the tissue
processor).
Press and hold the green button in the down position.
Switch on the instrument, maintaining the green button in the down position until such
time as the Main Menu appears on the LCD screen again.
After this operation, the ATP will be ready for operation again, but first the user MUST verify the
following:
That the retort is empty.

That the retort is clean (above all in reference to the paraffin).
That the lid gasket of the retort is clean and in its correct position.
That the instrument works correctly, by performing tests from the service menu.
That an alarm present before the reset is not again displayed (in this case will be necessary
to switch off the instrument and call the TBS service department, with the exception of an
alarm #1).
It is advisable to run a Clean Cycle and/or an abbreviated process before attempting a
sample run in order to verify the correct operation of the processor.
Be certain to perform the checklist (located in Chapter 10.8) before starting a program or a
Clean Cycle.
58
CHAPTER 12 SERVICE MENU

12.1 END USER SERVICE TESTS
The End-user Service Menu:
EDIT SETUP
Vacuum test
The instrument will create a vacuum as if a program is running; this test should be
done with the retort empty.
The instrument should reach a pressure between 300 and 350mBar (TP field)
within 50 to 60 seconds (GRADIENT field).
If the GRADIENT value exceeds 60 seconds, the Retort lid gasket should be checked
and cleaned. If this does not correct the problem, contact the TBS service
department for technical service.
59
Pressure Test
The instrument will create the pressure in the Retort as if a program is running; this
test should be done with the retort empty.
The instrument should create a pressure between 1200 and 1250mBar (TP
field) in a maximum time of 60 seconds (GRADIENT field).
If this does not happen, check and clean the Retort lid gasket; if the problem still
persists, contact our technical service.
Retort Heating
This test is to:
Check the heating elements for the Retort.

Melt the paraffin residuals that could be remaining in the Retort in case of an
alarm or a breakdown.
The Retort temperature is automatically brought to 60C.

This process may require up to 30 minutes. This test can be stopped at any time.
Runtime Test
The tests are the same as for the start of a program. This function is important to
check after a long period of inactivity.
Edit Setup
This is where you can set the following items:
Language
Reseller
Format for Display Date
Serial Baud 1200 is default
Elevation Important for high altitude laboratories
L/L Alarms Enable/Disable Low Level Alarms Beeping, Error codes
will still be displayed even if sound is disabled.
EOP Beeping Enable/Disable End of Process Beeping
POS Sensor only adjustable by service technician
Reset Flag
This function has the same effect as the Reset Button located on the rear panel of
the instrument.
Alarms Reset
This function is used to eliminate an alarm in order to run tests of the instrument. It is
also utilized after a problem has been corrected, (i.e. in order to continue on with
beginning processes, etc.) It is also useful to reset the alarms occurring during other
tests on the instrument.
60
If there has been a power loss or the unit has been powered off and on, the alarm
reset will need to be used to clear the Blackout Alarm.
12.2 SERVICE MENU ABBREVIATION LIST
On the Right side of the screen are a number of values identified by abbreviations
defined below:
POS
STEP
VR1-6
TP
OVERFILL
VACUUM
GRADIENT
ALARM
RETORT
/D
SETP RET
T DIR RET
T MED RET
T MAX RET
SETP WAX
T DIR WAX
T MED WAX
T MAX WAX
Rotary Valve position

Rotary Valve position required
Binary Rotary Valve position
Retort direct and average pressure
Overfill sensor status
Retort pressure management
Time necessary to make vacuum and pressure in the Retort
Running alarm code
Number of the current contents of the retort
Number of the last reagent in the Retort (0 If Clean Cycle Run)
Retort temperature set point
Retort direct temperature
Retort average temperature (on 20 direct reads)
Retort maximum temperature raised after the last reset
Paraffin Chamber temperature set point
Paraffin Chamber direct temperature
Paraffin Chamber average temperature (on 20 direct reads)
Paraffin Chamber maximum temperature raised after the last reset
12.3 SERVICE ASSISTANCE
Before calling TBS Product Service, please collect the following information:
Type of instrument
Serial number
Software release
The number of any alarm that has occurred
A print of the Alarm Archive (if the printer is available)
Please provide this information to Product Service upon contact. Our telephone
number is 919-384-9393. The department is staffed 8:00 AM to 6:00 PM EST or
DST.
61
CHAPTER 13 SAFETY FEATURES

13.1 PROTECTION
AGAINST
OVERHEATING
The thermostat controls include a maximum temperature cutoff switch to prevent

overheating of the paraffin chamber and Retort.
These devices do not prevent alarms, but, prevent overheating when primary control
devices fail. DO NOT use the instrument when an alarm indicating a strange
temperature increase is illuminated.
In the event of overheating, it is best to switch off the instrument and disconnect it
completely.
13.2 PROTECTION
AGAINST
EXCESS PRESSURE
There is no danger of excess pressure mishaps. The pressure levels developed in

the retort are small and do not present any major hazard.
A light pressure can be present in the retort at the end of a process.

The lid must be opened with care to avoid the possibility of eye injury by
reagent vapors and splashing. Always wear safety glasses when
handling reagents or working to identify problems.
62
CHAPTER 14 INSTRUMENT MAINTENANCE

14.1 DAILY MAINTENANCE
The daily maintenance of the instrument consists of:
Cleaning the Retort

Cleaning the Retort lid and gasket
Checking and possible replacement of reagent and paraffin
Checking and possible replacement of bottle caps
Checking that reagent and paraffin bottles are correctly seated and filled to
the proper level
14.2 MONTHLY MAINTENANCE

The following monthly maintenance is recommended:
Replace the charcoal filters as indicated in Chapter 6.3 and any time
there are excessive reagent fumes.
When replacing reagents, carefully clean the bottles
Grease the Retort lid gasket with silicone grease (Cat # 7PV999)
Grease the bottle insertion o-rings with silicone grease (Cat # 7PV999)
Check for reagent residues at the bottom of paraffin bottles.
Remember that both bottles and paraffin are at a temperature of
~60C; therefore contact with them can cause burns.
14.3 PARAFFIN WAX PURIFICATION CYCLE
An important addition to the ATP maintenance process is the Paraffin Wax
Purification Cycle, or WPC. Regular use of this feature will significantly reduce
paraffin consumption and thus time spent replacing or maintaining reagents. The
WPC works by using air and heat to vaporize contaminant reagent carryover in the
paraffin waxes and then trapping these vapors in the charcoal filters. Using this
process, the majority of xylenes carried over into the initial paraffin steps are
removed.
This purification process takes place in the paraffin bottles; the retort is
not filled with paraffin, so, unlike other tissue processes, it is not
necessary to run a Clean Cycle after the execution of the WPC.
Another advantage of conducting this process in the bottles is that it can be stopped
at any time to begin a new process without draining the retort and having to run a
Clean Cycle, thus saving 30 to 50 minutes.
To maximize the value of this process and to therefore minimize paraffin

consumption, the WPC should be run after every process.
63
Important note: The retort should be cleaned (using the Clean Cycle
following a process) prior to starting the WPC and the retort lid MUST
remain closed during the purification cycle.
Although there will be no paraffin in the retort, it is important to keep the retort closed
during the process to ensure proper functioning of the pneumatic (air) circuit. If you
must open the retort for some reason or if you wish to start a process before the
WPC is complete, use the ESC key to halt the WPC before opening the lid.
14.4 CHARCOAL FILTERS REPLACEMENT
Filter replacement is required approximately every 60 to 120 processes, based upon

environmental factors and use of the WPC. We estimate that:
Without the use of the WPC, filter life will be from 90 - 120 processes
With the use of the WPC, filter life will be from 60 - 90 processes
These values are only estimates because they are influenced by:
Environmental factors (humidity, temperature)

The duration of the processes
The application of the vacuum (or pressure) on all the steps of the process
The frequency of reagent agitation
Regardless of usage, the charcoal filters should be changed at least once every six
months. To order additional filters, specify Cat # 8A1850.
Exhausted filters must be replaced with the required frequency as they may release
toxic and contaminated vapors into the air if left too long.
Exhausted filter waste is to be handled in accordance with local, state and national
regulations.
14.5 RETORT LID GASKET REPLACEMENT
The Retort lid gasket is made of VITON rubber (a DuPont trademark). It needs to
be periodically greased with TEFLON grease (Cat # 7PV999). When replacing, use
a sharp point not a metallic one to extract the gasket. Carefully clean the gasket
housing slot and replace the gasket with a new one.
64
14.6 FUSE REPLACEMENT
Fuse replacement should be done only by qualified personnel.
Please note: Always disconnect the instrument! Never use fuses of a

different rating and never try to repair damaged fuses.
The ATP contains four (4) line voltage fuses. Fuses are in fuse holders located on
the rear panel. See the illustration in Chapter 3.3 for details.
There are 4 glass fuses of a 10 Amp (6 x 25 mm) rating in holders at the rear or the
unit.
To replace fuses:
1. Disconnect the cable from the socket on the power entry module.
2. Open the small door under the socket with your fingers or a screwdriver and
extract the holder containing the fuses with the help of a pair of tweezers.
3. Replace the fuses.
4. Replace the Fuse holder in the instrument.
5. Reconnect the power cable.
65
CHAPTER 15 ACCESSORIES
15.1 PRINTER
The ATP is equipped with a serial port in the back panel for the connection of a
printer. TBS offers a printer specifically for the ATP (cat # ATP1-P), or, any kind
of serial printer with the following characteristics can be used:
At least 40 columns
Speed of the serial port adjustable to 600 Baud
Speed of printing 15 cps or greater
Serial cable with 9 poles Canon connector
Electric autonomous feeding
Features of the ATP specific printer (Star DP 8340):
40 columns
Printing on normal paper
Roll of inside paper, of 25m., of easy substitution
Cartridge to inky ribbon of easy substitution
Extreme compactness (could be positioned on the cover of the ATP)
Speed of printing of 20 cps
Electric autonomous feeding
CE certification and UL
Serial cable
Specific manual of use and maintenance
For the use of the printer, please consult its specific manual.
The pin usage of the ATP serial port is the following:
ATP SERIAL PORT
1 - DCD
2 - RD
3 - SD
4 - DTR
5 - GND
6 - DSR
7 - RTS
8 - CTS
9 - RI
DESCRIPTION
Unused
PRINTER CONNECTOR
TX
RX
Unused
GND
DTR
Unused
RTS
Unused
Please note: The manufacturer declines any responsibility for the correct operation
of printers other than specified in the present manual.
66
15.2 REMOTE ALARM
AND
AUTO-DIALER
An automated dialer can be attached which will automatically dial a pre-set number if
an alarm occurs during a run.
The socket of the Remote Alarm connector has 3 contacts with the following
disposition:
No alarm
= contacts 1-2 closed, contacts 1-3 open.
Alarm in course
= contacts 1-2 open, contacts 1-3 closed.
Because there are no voltages of any kind present on these contacts (they are
isolated from the rest of the instrument), they can be utilized to activate an external
device such as an Auto-Dialer to call a pre-selected number and relay the message
that an alarm has occurred.
If you wish to attach such a device to the ATP but do not wish to purchase our
Auto-Dialer, or, if you have a proprietary system already in place, please note the
following constraints on the connector and its associated electronics:
Maximum allowed voltage: 48V AC/DC

Maximum allowed current: 6A
For instructions related to the Auto-Dialer, please consult the manual included with
the dialer.
67
APPENDIX 1 ACCESSORIES AND SPARE PARTS

DESCRIPTION
U/M
CAT#
Ea
ATP1-AD
Ea
ATP1-P
CS
ATP-CFB
Paraffin/reagent bottles (reusable, quick connector) 6/CS

CS
Paraffin/reagent bottles (reusable, bottles only no caps or
Ea
connectors)
Screw caps for reagent bottles, solid 10/CS
CS
ATP1-BR
Screw caps with cut-out for Quick Coupler Fitting

Basket, regular, cassette capacity 150 (with: 1 cover, 4
dividers, and 4 springs)
Basket, bulk, cassette capacity 200-220 random or 350
stacked (with: 1 cover)
Carrier Handle for easy movement of baskets (fits 2 ATP1B150, or 1 ATP1-BB)
Paper roll for printer 12/Cs
Ea
ATP1-177
Ea
ATP1-B150
Ea
ATP1-BB
Ea
ATP1-259
Cs
ATP1-PP
Ink ribbon for printer 6/Cs
Cs
ATP1-PR
ATP user manual (English)
Ea
ATP1-OM
Main power cord
Ea
43177R
Fuses, 10A, 250V
Pair
F483-ND
Retort lid gasket
Ea
ATP1-205
Quick Coupler O-Rings Small (2 per coupler)
Ea
9464K65
Quick coupler O-Rings Large (1 per coupler)
Ea
9464K34
ATP service manual (English)
Ea
ATP1-SM
Accessories
Auto Dialer
Printer (with: main power cord, serial cable, 1 paper roll, 1
ink ribbon, user manual)
Charcoal filters 2/CS
ATP1-123
ATP1-BC
User Replaceable Spare Parts
For the complete list of spare parts, see the Technical and/or Service Manual.
68
APPENDIX 2 ATP TECHNICAL DATA SHEET

Rating
Max power
Fuse
Weight
Dimension in mm (floor standing version)
Dimension in mm (table top version)
Height
Width
Depth
Running ambient temperature
Storing temperature
Sample processing capacity
Paraffin temperature (warm chamber)
Paraffin temperature (during the process)
Reagent temperature (during the process)
Temperature accuracy
Reagent filling time
Reagent draining time
Pressure range
Filling pressure
Draining pressure
220V, 50Hz; 110V, 60Hz

1400 W
2x10A (5x20mm)+ 2x10A (6x25mm)
120 Kg (264 lbs.)
H 1338 - W 524 - D 600
H 770 - W 1048 - D 600
130 cm (33 in)
53 cm (13.5 in)
60 cm (15 in)
10 / 30 C
0 / 40 C
300
50 / 60 C
50 / 60 C
Ambient / 45 C
+/- 1 C
45 s
30 s
300/1350 mBar (ambient = 1000 mBar)
750 mBar
1250 mBar
69
APPENDIX 3 ATP FEATURES

Electronics
Software language
Display
Shell (user interface)
Number of programs
Program features
Anti-blackout device
Reagents selection
Number of reagents
Number of paraffin
Pre-heated paraffin
Number of Purge
reagents
Number of filters
Retort
Purge duration
Purge steps
Sample capacity
Sample basket
Based on an industrial CPU with an Intel microprocessor

Microsoft C 6.00
9.5 LCD - VGA mono tilting touch screen (panel-off feature
to increase the life of the display and the display light)
User friendly (Windows like) shell; simple and clear menu
guides the user over the running of the instrument; all the
events are guided by 2 alert rows; temperatures and
pressures are always displayed on the monitor
12 + Reversed (14 steps each)
Max step time: 12 hours. Up to 7 days delay; start of a
process from any step; process can be aborted at any step.
The CPU memory is battery backed-up. In the event of a
blackout, the processor restarts the process from the
interrupted point. In the event a BO stops the process during
a paraffin step, a delay function allows sufficient time for
paraffin melting before restarting the program.
All reagents and paraffin are selected by a rotating valve
10 (contained in a 2.5 lt. bottle)
4 (contained in a 2.5 lt. Bottle)
1 slot
2 (contained in a 2.5 lt. Bottle)
2 charcoal (contained in a 2.5 lt. modified bottle)
Stainless steel
From 28 to 48 minutes
3 (xylene, alcohol 95%, drying)
Up to 300 samples (250 organized)
2x stainless steel - 150 samples each
(125 samples each with spring organizers)
1x stainless steel - 200 samples random
70
APPENDIX 4 ATP PROGRAM MENU FLOW
71
CONTACT INFORMATION
Customer service
(919) 384-9393
Fax
(919) 384-9595
Mailing address
TBS - A Division of General Data Healthcare, Inc.

3014 Croasdaile Drive
Durham, NC 27705 U.S.A.
[email protected]
Sales and product information

Product Service questions
All other inquiries
[email protected]
[email protected]
Please visit us at
http://www.trianglebiomedical.com
72
General Data Healthcare helps labs enhance
More Information
productivity and improve workflow with
PH: 800.733.5252 ext. 6473
innovative products, solutions and support that

focus on delivering better patient safety & care.
www.trianglebiomedical.com
2013 General Data Healthcare, Inc. All rights reserved. Specifications are current at the time of publication; however, they are subject to change without notification. Please contact our North Carolina
office or one of our distributors for current prices and availability. All trademarks and registered trademarks are property of General Data Healthcare, Inc., Cincinnati, Ohio, USA.
A Division of General Data Healthcare
TBS A Division of General Data Healthcare

TBS is a Division of General Data Healthcare
3014 Croasdaile Drive, Durham, NC 27705
4354 Ferguson Drive, Cincinnati, OH 45245
Phone: 919.384.9393 Fax: 919.384.9595
Phone: 800.733.5252 ext. 6473
Email: [email protected]
Email: [email protected]
73
www.trianglebiomedical.com
www.general-data.com/hc

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Pub No.

Histology Innovation for a NEW Generation

ATP Tissue Processor

TBS Corporate Headquarters

Product Service Department

Copyright 2013 General Data Healthcare, Inc.

7.5 Program Label .............................................................................................. 21

10.3 Selecting a Program ................................................................................... 41

26 - RET. NOT EMPTY...................................................................................... 55

APPENDIX 1 ACCESSORIES AND SPARE PARTS ......................................... 68

Copyright 2013 General Data Healthcare, Inc.

Copyright 2013 General Data Healthcare, Inc.

CHAPTER 1 GENERAL INFORMATION

The instrument should only be operated by qualified technicians.

1.1 REGULATORY COMPLIANCE

Copyright 2013 General Data Healthcare, Inc.

1.2 PATENTS, TRADEMARKS

Do not use free flame near the instrument

No smoking near the instrument

Important safety notice

Important operation information

Copyright 2013 General Data Healthcare, Inc.

Paraffin Wax Heating Chamber

Pressure unit of measure

Copyright 2013 General Data Healthcare, Inc.

1.5 SAFETY PRECAUTIONS

Always make certain to correctly engage the reagent bottles.

NEVER use ACETONE, BENZENE, or TRICHLOROETHANE.

Use specific precautions in handling flammable reagents such as ethanol.

Copyright 2013 General Data Healthcare, Inc.

Due to the presence of flammable substances inside reagent bottles, it is

Avoid smoking near the instrument.

Contaminated reagent waste must be disposed of in accordance with all

Access to the instruments internal components is reserved only to those

Always disconnect the processor from the electrical source before

Only original spare parts sold by the manufacturer or by authorized dealers

BEFORE replacing fuses, disconnect the instrument from the power

DO NOT extract bottles, replace reagents or do other maintenance

Copyright 2013 General Data Healthcare, Inc.

CHAPTER 2 UNPACKING, PACKING AND TRANSPORTING

The proper steps to be taken for unpacking are:

Copyright 2013 General Data Healthcare, Inc.

To transport the instrument, perform the following steps:

1) Remove all reagents (paraffin included) from their bottles.

1) The instrument is very fragile.

Copyright 2013 General Data Healthcare, Inc.

CHAPTER 3 INTRODUCTION TO THE ATP PROCESSOR

The control devices (hardware and software) are based on up-to-date

Up to 12 different programs can be permanently stored and can be easily

Copyright 2013 General Data Healthcare, Inc.

3.2 FRONT VIEW

Tilting color touch

Copyright 2013 General Data Healthcare, Inc.

3.3 REAR PANEL & FLOPPY DRIVE

On the rear panel of the control module there are:

A = On/Off main switch

= pins 1-2 open, pins 1-3 closed

Please note: The socket at E is a low voltage connection (maximum 48V,

4.1 COMPATIBLE REAGENTS