Fluido 2 Manual

Fluido 2
Microplate Washer
Operator’s manual
Biochrom Ltd
22 Cambridge Science Park
Cambridge
UK
CB4 0FJ
Tel.: +44/1223 423723

E-Mail: [email protected]
June 2008 Fluido 2 Page 1

Table of Contents
Introduction......................................................................................................... 4
2 Warnings and Hazards....................................................................................... 5
2.1.1 Safety symbols ..................................................................................................... 6
3 Precautions .......................................................................................................... 7
3.1 Cleaning the instrument........................................................................................ 7
4 Specifications ....................................................................................................... 8
4.1 Declaration of Conformity ................................. Error! Bookmark not defined.
4.2 Metrological specification .................................................................................... 8
4.2.1 Rated operating conditions ................................................................................... 8
4.2.1 Limits of error and tolerance limits ...................................................................... 8
4.3 General technical data .......................................................................................... 8
4.3.1 Dimensions ........................................................................................................... 8
4.3.2 Power supply ........................................................................................................ 8
4.3.3 Interfacing ............................................................................................................ 9
4.3.4 Options ................................................................................................................. 9
4.4 Cleaning................................................................................................................ 9
4.5 General features.................................................................................................... 9
4.6 Standard delivery content ................................................................................... 11
4.7 Warnings and directions ..................................................................................... 11
4.8 Unpacking Procedure ......................................................................................... 13
4.9 Installation requirements, Environmental operating conditions......................... 13
4.10 Instrument installation procedure ....................................................................... 14
4.10.1 Connecting the instrument with an external computer....................................... 15
4.11 Re-packing to prevent damage during transport ................................................ 16
5 Theory of Operation ......................................................................................... 17
5.1 Introduction ........................................................................................................ 17
5.2 Main components ............................................................................................... 17
5.3 Hydraulic system ................................................................................................ 17
5.3.1 Dispensing system .............................................................................................. 17
5.3.2 Aspirating system ............................................................................................... 17
6 Warranty ........................................................................................................... 19
6.1 Liability .............................................................................................................. 19
7 Software Principle............................................................................................. 20
7.1 Software Navigation........................................................................................... 20
7.2 Programming Sequence...................................................................................... 21
7.3 Menus ................................................................................................................. 22
7.3.1 Run Procedure .................................................................................................... 23
7.3.2 Prime / Rinse ...................................................................................................... 24
7.3.3 Define Procedure ................................................................................................ 25
7.3.4 Define Cycle....................................................................................................... 26
7.3.5 Define Plate ........................................................................................................ 27
7.3.6 Setup................................................................................................................... 28
7.3.7 Service ................................................................................................................ 29
7.4 Description of the key functions ........................................................................ 30
8 Start-up and system setup ................................................................................ 31
8.1 Start-Up .............................................................................................................. 31
8.2 Display................................................................................................................ 31
8.3 Control of the system setup ................................................................................ 31
9 Programming..................................................................................................... 32
9.1 Run Procedure .................................................................................................... 32
9.2 Prime/Rinse Menu.............................................................................................. 34
9.2.1 Manual Prime/Rinse ........................................................................................... 34
9.2.2 Automatic Prime/Rinse ...................................................................................... 35
9.2.3 Periodic Prime/Rinse.......................................................................................... 36
9.3 Define Procedure Menu...................................................................................... 38
9.4 Define Cycle Menu ............................................................................................ 43
9.5 Define Plate Menu.............................................................................................. 48
9.6 Setup Menu......................................................................................................... 58
9.7 Service Menu...................................................................................................... 61
10 ERROR MESSAGES ....................................................................................... 62
10.1.1 Duplicate Name .................................................................................................. 62
10.1.2 Wrong Manifold ................................................................................................. 62
10.1.3 No Cycle Defined ............................................................................................... 62
10.1.4 No Plate Defined ................................................................................................ 62
10.1.5 Waste Full........................................................................................................... 63
10.1.6 Liquid Empty...................................................................................................... 63
10.1.7 Stop Pressed ....................................................................................................... 64
10.1.8 Plate Transport Error .......................................................................................... 64
10.1.9 Manifold Transport Error ................................................................................... 64
11 Maintenance ...................................................................................................... 65
11.1 Recommended regular inspection ...................................................................... 65
11.2 Approved parts ................................................................................................... 65
11.3 Exchanging a Fuse.............................................................................................. 65
11.4 Cleaning the instrument...................................................................................... 66
11.5 Disinfection Procedure ....................................................................................... 66
12 Intended Use ...................................................................................................... 68
12.1 Quality control.................................................................................................... 68

1 Introduction
The Fluido 2 is a fully automated, programmable 96-well

microplate washer with 8- or 12-way manifolds. An optional 16-
channel manifold allows to wash 384-well microplates.
The Fluido 2 is designed around your application. It incorporates
state of the art technology and software both necessary to allow
optimum performance and easy use.
Easy operation through the menu structure of the on-board
software. The display and the keyboard provides clear access to all
features of the flexible software
The Fluido 2 can be operated with up to 3 different wash liquids to
be selected by software.
An easy plate setup is provided to optimise the movement of the
plate and the manifold according to the different dimensions of
different plate types.

2 Warnings and Hazards
General:
If inflammable, toxic or biologically hazardous substances are
used when operating the equipment, please observe the
instructions and precautions enclosed with such substance.
Never spill fluids in or on the equipment.
Wash your hands thoroughly after handling test fluids.
If equipment has been in contact with hazardous substances, it
must be disinfected prior to shipment in accordance with the
effective provisions.
Do not touch the plate during movement of the plate transport
(risk of injury).
The equipment may not be opened other than as described in
chapter 11 Maintenance, since it contains live parts (danger of
life).
Electrical:
Voltages dangerous to human life are present in this device.
Before removing any covers disconnect the device from the
power source.
Ensure that the power cord supplied with the unit is used.
The power cord may only be inserted in a socket outlet
provided with a protective ground (earth) contact. The
protective action must not be negated by use of an extension
cord without a protective grounding contact.
Do not replace fuses without first removing the main power
cord.
Ensure that only fuses with the required rated current and of the
specified type are used for replacement. The use of makeshift
fuses and the short-circuiting of fuse-holders is prohibited.
When the apparatus is connected to the main power source, the
opening of the covers or removal of components is likely to
expose life parts. The device shall be disconnected from all
voltage sources before it is opened for adjustment or repair.
Any adjustment or repair of the opened apparatus under voltage
should be avoided, but, if necessary, it must be carried out by
qualified service personnel who are aware of the hazards
involved.

Magnetic:
The removable plate carriage of this instrument has a very
strong permanent magnet mounted. Be sure to never bring any
other magnets or Ferro-magnetic parts near to the magnet of the
plate carriage (risk of injury).
Never place credit cards or IDs or any other items using
magnet-code (floppy disks, audio- or video tapes, ...) near to the
magnet of the plate carriage (risk of data loss).
Be careful when placing the plate carriage onto other sensitive
medical devices, which may be influenced by magnetic fields.
2.1.1 Safety symbols
The following safety symbols may be found in several locations on the instrument. Only
persons who fully understand the safety precautions and recognize shock hazards should
operate this instrument.
~ Alternating current
In Vitro Diagnostic Device
Protective ground terminal
ON
OFF
Caution, see enclosed document
The symbol on the product indicates that this product shall not be
treated as household waste. Instead it shall be handed over to the
applicable collection point for the recycling of electrical and electronic
equipment.

3 Precautions
3.1 Cleaning the instrument
This instrument is a precision instrument and it requires regular

cleaning to ensure the continued precision.
Liquid Spills
If any liquid is spilled on the instrument, it should be
IMMEDIATELY removed so that the liquid does not attack the
surface of the housing.
Regular cleaning
The housing of the instrument should be cleaned regularly with a
mild household cleaning agent.
Warning: Do not use aggressive solutions!
The area surrounding the plate carrier should be cleaned with a dry
cloth.
Fittings and tubing
The usual wash buffer tends to crystallize easily on air and can then
block tubing and fittings. Use the automatic rinse program daily
and do not leave the tubing filled with wash buffer for an extended
period of time.

4 Specifications
4.1 Metrological specification
4.1.1 Rated operating conditions
Warming-up time: Ready for operation immediately

after switching-on.
Operating voltage: 90V - 130V,
180V – 250V autosensing
Fuses (user exchangeable): 2 pcs. T 2.5 A, 250V
Built in Fuse (on power supply): 1 pcs. F 2A, 250V, fa
Display: 2x16 characters display (with
backlight)
Keyboard: Keyboard with 5 function keys
Ambient temperature: 15°C - +40°C (operation)
-25°C - +50°C (storage)
Relative humidity: 15 - 85% non-condensing
(operation)
< 95% non-condensing (storage)
Air pressure tolerance: 54.000 - 106.000 Pascal
Maximum altitude (operation): up to 5000 m
4.1.1 Limits of error and tolerance limits
Dispense volume: wash liquid volume adjustable

from 50 - 2000µl in increments of
50µl
Dispensing accuracy: 5% at 300µl across the plate
Residual volume: 1µl per well
The tolerances of the measuring devices are based on the
manufacturer’s information.
4.2 General technical data
4.2.1 Dimensions
Weight: 6,2 kg net

Dimensions (width x length x height): 21 cm x 46 cm x 21 cm
4.2.2 Power supply
Consumption: 80VA (maximum)

Operating voltage: 90-130V, 180-250VAC (autosensing),
frequency range: 47 - 63 Hz

4.2.3 Interfacing
Serial interface (RS232 9-pin male connector) for remote control

of basic wash functions, instrument status and setup by external
PC (with null-modem serial cable used for PC connection).
4.2.4 Options
liquid level sensors for wash and waste bottles

8- and 12-way manifold for 96-well plates
16-way manifold for 384-well plates 
Serial interface cable
4.3 Cleaning
This instrument is a precision instrument and it requires regular

Liquid spillage
Regular cleaning
cloth.
Fittings and tubing
The usual wash buffer tends to crystallize easily on air and can then
block tubing and fittings. Use the automatic rinse program daily
and do not leave the tubing filled with wash buffer for an extended
period of time.
4.4 General features
The Fluido 2 incorporates state of the art technology and software

both necessary to allow optimum performance and easy use.
The Fluido 2 is designed around your application.
Easy operation
Through the menu structure of the on-board software. The display
(2x16 characters) and the keyboard with 5 keys provides clear
access to all features of the flexible software. If desired the washer
may also be operated from a PC using the optional Windows based
software.
8 and 12-way manifold
as standard configuration. Additional manifolds available as option.
The manifold is easily exchanged. Depending on the type of strip-
plate (8- or 12-way strips) the corresponding manifold is used to
wash a selected number of strips or the complete plate.
Up to 3 different wash liquids
The basic version incorporates 1 liquid line for wash buffer, rinse
liquid and waste. The extended version accesses to up to 3 different
wash buffers connected to the instrument which can be selected by
software.
Vacuum and pressure free system
Any appropriate container can be used to hold the wash buffer. No
special bottles are required.
Quiet operation
The Fluido 2 is very quiet due to the special pumps used.
Additionally the pumps are only operating when the washing
procedure is performed.
Automatic periodic rinse
may be activated in order to avoid clogging if the washer is not
operated.
Aerosol Protection
Covers the manifold and the rear plate position. It can easily be
removed for cleaning and maintenance.
Easy access to all tubes and valves
All valves and tubes which may require handling by the user are
easily accessible to assure fast and convenient maintenance.
Plate setup
Is used to optimize the movement of the plate and manifold
according to the specific dimensions of different plate types.
Residual volume is minimized by the crosswise aspirate function
which aspirates in the edges of the well. Up to 20 different plate
types (flat- and round-bottom) can be configured and stored on the
instrument.
Bottom Detection
As part of the plate setup this unique feature supports optimization
of plate specific parameters. The instrument is able to automatically
detect the bottom of the well. This results in improved washing
performance and minimized residual volume without the manifold
touching the bottom of the well (with flat bottom plates only).
Wash cycles and procedures
Wash cycles: Up to 20 may be defined according to specific
requirements and are stored on the instrument. Once a wash
cycle is created it may be used in different wash procedures.
Some of the main features are:
• Bottom and Overflow washing
• Adjustable Dispense volume and speed
• Adjustable aspiration power and time
• Adjustable soak time
• Shaking (3 modes)
Wash procedures: Up to 50 may be created by putting up to 8
previously defined wash cycles in sequence. Washing
procedures can either be performed step by step over the whole
plate (PLATE-mode) or strip by strip (STRIP-mode). The
number of strips to be processed may be defined at the time the
wash procedure is started.
Options
Liquid level detectors to check volumes in wash and waste
bottles.
Remote control software for PC:
Windows based software for programming of washing
procedures and plate configurations. The washer may be
remotely controlled from the PC to run the instrument in a
Windows environment. Data may be transferred between
instrument and PC.
The combination of both platforms, the flexible onboard
software and the convenient Windows-based program gives the
user the optimum choice to operate the Fluido 2.
Software updates on the washer are performed conveniently
from the computer. Existing procedures or plate setups can
easily be copied to another unit without the need to go through
the programming again.
4.5 Standard delivery content
Washer Fluido 2
external tubing set
Manifold (8- and 12-way)
1 or 3 wash bottles 2l (depending on configuration)
Rinse bottle 2l
Waste bottle 2,5l
Power cord
User manual
Spare fuses
Cleaning tools for needles
4.6 Warnings and directions
If inflammable, toxic or biologically hazardous substances are used

when operating the equipment, please observe the instructions and
precautions enclosed with such substances.

Never spill fluids in or on the equipment. Wash your hands
thoroughly after handling test fluids.
If the equipment has been in contact with hazardous substances, it
must be disinfected prior to shipment in accordance with the
effective provisions.
Do not touch the plate during movement of the plate transport (risk
of injury).
The equipment may not be opened other than as described in the
chapter 11 Maintenance, since it contains live parts (danger to life).

4.7 Unpacking Procedure
1. Visually inspect, the container for damage, before opening it.

Note: Report any damage immediately to the forwarding agent or to the delivery
carrier.
2. Place the carton in an upright position and open it.
3. Remove the upper cardboard box carefully.
4. Lift the instrument out of the carton and place it on a flat
surface, free from dust, vibration and away from direct sunlight.
5. Visually inspect the instrument for loose, bent or broken parts.
Note: Report any damage immediately.
6. Compare the instrument's serial number, attached on the rear
panel of the instrument, against the serial number of the
instrument on the delivery (shipping) note and packing list.
6. Check the instrument accessories against the delivery (shipping)
note and packing list.
7. Please save all packing materials, as they maybe required
for later transportation.
4.8 Installation requirements, Environmental operating

conditions
Flat, dry, clean and vibration-proof working area (50 cm x 35

cm - length x width)
Additional room for cables, connections, etc.
No direct sunlight
Clean ambient air (free of corrosive vapours, smoke and dust)
Ambient temperature between 15°C and 40°C
Humidity between 15% and 85%.
Before the instrument is installed and switched on, it should be
left to stand for at least 2 hours, so there is no possibility of
condensation causing a damage or malfunction.
Use the power cable supplied with the instrument to connect the
instrument's main plug with a power outlet providing protective
earth.
Please Note::
Only use the power cable supplied with the instrument or a power cable with
protective earth connection carrying the CE-mark.
The power outlet the instrument is connected to has to meet the applicable
technical safety requirements!

4.9 Instrument installation procedure
The following procedures detail the necessary steps to be followed

when installing the Instrument.
1. Place the instrument into the required position
Note: Make sure the distance between the back panel of the instrument and the
wall, is at least 5 cm.
Remove all packing material and transport lock tapes.
2. Remove all packing material from the manifold. Look for the
two manifold connecting tubes, packed together with the
manifold. Connect the aspiration tube with the red quick fit
connector to the corresponding connector at the top plate of the
washer and slide the other end of the tubing on the red
connector of the manifold. Now connect the thin dispense
tubing with the blue quick fit connector to the corresponding
connector. Press down now the black pinch valve actuator and
slide the dispense tube into the side slot of the pinch valve.
Make sure that the tubing is fully inside the valve. Afterwards
slide the dispense tube on the blue connector of the manifold.
Place the manifold now on the horizontal manifold holder bar so
that the two holding pins are resting in the slots of the manifold
holder.
3. Connect the long aspiration tube with the red fitting to the
connector at the backside of the washer and to the waste bottle.
4. Connect the wash fluid tube with blue fitting to the connector at
the backside of the washer and to the wash bottle.
5. Connect the rinse fluid tube with the white fitting to the
connector at the backside of the washer and to the rinse bottle.
6. Ensure that the mains power switch at the back panel of the
instrument is in the OFF position.
7. Insert the power cable into the mains power socket in the back
panel of the instrument.
8. Switch the washer on.
If the instrument is turned on using the power switch on the rear
side of the device the green power indicator on the left front side of
the device has to be illuminated.
When connecting the instrument with an external computer make
sure the instrument is switched off (the power indicator is dark). If
the instrument is turned on turn it off using the power switch on the
rear side of the device.

The power indicator is located on the front left side of the unit and
is marked with the following symbol.
The stand-by indicator may show 3 different states:

Green light: Instrument is in stand-by (ready for operation).
Not illuminated: Instrument is not ready for operation.
Please Note:
The power indicator does not indicate that the instrument is turned on or off or
that it is connected with mains but only that the instrument is ready for
operation!
In order to make sure the instrument is disconnected from power
the main switch on the rear side of the unit has to be switched to
the "OFF-position" indicated with a "0" symbol. Alternatively the
power cord can be disconnected from the unit.
4.9.1 Connecting the instrument with an external computer
Turn the instrument off.

Connect the serial interface (9-pin DB9 connector) on the computer
with the connector at one end of the serial cable supplied . Lock the
connector to the plug using the corresponding screws on the
connector.
Connect the other end of the serial cable with the plug marked
"RS232" on the rear side of the instrument. Lock the connector to
the plug using the corresponding screws on the connector.
Please Note:
Only use the original cable supplied with Fluido 2.
Other cables with identical connectors may not be used to establish
communication between Fluido 2 and a computer!

4.10 Re-packing to prevent damage during transport
The original Fluido 2 packing has been specially designed for this
equipment.
1. Remove the manifold and tubing and the plate carriage and
pack them into the accessories boxes.
2. Lock the holder of the manifold with the foam part as shown in
picture 1.
3. Put the transport lock plate at the location shown in picture 2
and secure the plate with adhesive tape.
4. Place the foam parts left and right on the washer and put the
washer into the transport box. (picture 3).
5. Place the the bottles and the accessory boxes into the spaces left
and right of the instrument as shown on picture 3.
It is therefore recommended to save the original carton with its foam parts and
accessories box. Warranty claims are void if transport damages are caused by
improper packing!
picture 1 picture 2
picture 3

5 Theory of Operation
5.1 Introduction
This chapter gives the description of the main components of the

instrument and describes the hydraulic system.
5.2 Main components
The main components of the washer are the control electronics, the
plate transport mechanism, the manifold transport, the dispensing
and aspirating pumps, the keyboard with the LCD display and the
power supply unit.
5.3 Hydraulic system
5.3.1 Dispensing system
The dispensing system consists of the input selection valves, the

diaphragm pump, the damping tube, the pinch valve and the
manifold. The input selection valves are connecting the respective
liquid reservoirs (wash and rinse solution) to the internal dispense
circuits. The valves are controlled by the washer software to select
the correct liquid. The dispensing pump delivers the liquid to the
manifold, the operating time of the pump together with the
operating time of the pinch valve are controlling the programmed
volume.
5.3.2 Aspirating system
The aspiration is performed by a liquid pump connected to the

aspiration needles of the manifold. Compared to the traditional
vacuum pump operation this method produces much less noise and
does not require tight, closed bottles. Any kind of container with
sufficient volume can be used for the waste. In addition the risk of
infectious aerosols is much reduced.

Diagram 1.: Hydraulic Diagram

6 Warranty
Warranty shall lose effect if:

 Fluido 2 is not used in the defined scope of application,
 Fluido 2 has obviously been damaged by external influences
which are not in accordance with the provisions for the nominal
range of use,
 Fluido 2 has been modified or parts exchanged by a person other
than authorised personnel or an authorised servicing agent,
the warranty seals on the housing of the instrument are broken,
parts and subassemblies are implemented, which are not original
from Anthos.
the |Fluido 2 serial number is no longer legible, has been
removed or altered,
The Fluido 2 has not been installed in accordance with the
instructions supplied,
The Fluido 2 has been damaged during return transport due to
wrong packing (e.g. not in original packing material).
the Fluido 2 was damaged due to wrong operation, not according
to the descriptions in the manual.
The instrument may only be operated by qualified personnel.
If a warranty is brought into operation, Anthos shall repair or
replace any defects which have resulted from faulty material or
during production as it check fit. No costs shall arise for the client
(except cargo rates).
All components found in the original equipment, or an adequate
and full compatible alternative shall be available for a period of 5
(five) years after production.
This warranty refers to the obligations of Anthos and can only be
amended upon the written consent of Anthos.
6.1 Liability
In original condition the instrument meets all safety regulations for

a risk-less operation.
Anthos cannot warrant damages or any resulting costs caused by
alterations, repairs or modifications of the equipment.

7 Software Principle
7.1 Software Navigation
Drawing 2.: Software Navigation
The software was designed to make navigation through the

different menus as easy as possible. The main menus are organized
like a wheel which can be turned in both directions by means of the
two first keys (function key 1 <F1> and function key 2 <F2>).
When pressing <F1> or <F2> the next respectively the previous
menu will be displayed.
The two function keys may have different functions assigned in the
various sub-menus. The current assignment is always displayed
right above the corresponding key.
The lower right of the display will show the current selection of
different options (e.g. "Proc1" for the procedure with this name).
To change the selection use the <+> and <-> keys. These keys can
also be used for numerical or alphanumerical inputs by means of
in- or decreasing a value or scrolling through the alphabet.
By pressing <enter> the current selection is confirmed and the
software leads to the next menu to allow further selections.

7.2 Programming Sequence
The software offers the possibility to program up to 50 so-called

procedures (a procedure defines a complete wash program). To
define a procedure cycles (defines one part - cycle - of a wash
program; e.g. bottom wash) and plates (defines the shape and
measures of the plate used) are necessary.
Drawing 3.: Software Principle

Note: Please make sure to program the cycle and plate BEFORE the procedure!

7.3 Menus
The following flow diagrams give an overview of all menus and

the way of navigating through them. Detailed information for all
menus and their functions can be found in the following chapters.
Run Procedure:
<< >> Proc1 For details please refer to chapter 9.1 Run Procedure
Prime/Rinse:
<< >> Automat For details please refer to chapter 9.2 Prime/Rinse Menu
Def Procedure:
<< >> <new> For details please refer to chapter 9.3 Define Procedure Menu
Def Cycle:
<< >> <new> For details please refer to chapter 9.4 Define Cycle Menu
Def Plate:
<< >> <new> For details please refer to chapter 9.5 Define Plate Menu
Setup
<< >> For details please refer to chapter 9.6 Setup Menu
Service
<< >>
For details please refer to chapter 9.7 Service Menu

7.3.1 Run Procedure
<< Run >>

Procedure
<-|
Esc Nr of strips if 'Ask strip

nr' = No
<-|
No W ashliquid OK? if disabled
Yes
No W aste em pty? if disabled
Yes
No Plate inserted? if disabled
Yes
Perform
Stop
Procedure
Table 4.: Run Procedure

For details please refer to chapter 9.1 Run Procedure

7.3.2 Prime / Rinse
<< >>
Prime/Rinse
+/-
Esc
Esc
Manual <-| Manual
Liquid <-|
prime/rinse
+/-
Esc Esc
Automatic
Automat <-| Liquid <-| Volume
Rinse
+/-
Esc Esc Esc

Stop
Rinse
Period <-| Liquid <-| Volume <-| Periodic Rinse
interval
+/-
Table 5.: Prime / Rinse

For details please refer to chapter 9.2

7.3.3 Define Procedure
<< >>
D e f P ro c e d u re
< -|
E sc Nam e
< -|
<<
E sc L iq u id
< -|
<<
E sc C yc le 1 - 8 C yc le s
< -|
<<
E sc F in a l a sp tim e
< -|
<<
F in a l a sp
E sc if 'F in a l a sp tim e ' =
power
< -|
<<
P ro c e ssin g
E sc
m ode
< -|
<<
E sc P la te
< -|
<<
E sc A sk strip n r
< -|
<< if 'P ro c e ssin g

E sc D e la y fin a l a sp m o d e ' = 'S trip '
A N D 'F in a l a sp
tim e ' > 0
< -|
S to rin g
Table 6.: Define Procedure

For details please refer to chapter 9.3 Define Procedure Menu

7.3.4 Define Cycle
<< >>
D e f C ycle
< -|
E sc N am e
< -|
<<
E sc Asp ir tim e
< -|
<<
E sc A sp ir p o we r if 'A sp ir tim e ' = 0
< -|
<<
E sc D isp vo lu m e
< -|
<<
E sc D isp po w er
if 'D isp
< -|
vo lu m e ' = 0
<<
E sc W ash m o d e
< -|
<<
E sc Sh a kin g tim e
< -|
S h akin g << if 'S h ak in g

E sc tim e' = 0
in te n sity
< -|
M in im um soa k <<
E sc tim e
< -|
S to rin g
Table 7.: Define Cycle

For details please refer to chapter 9.4 Define Cycle Menu

7.3.5 Define Plate
<< >>
D e f P l a te
< -|
E sc Nam e
< -|
<<
Esc P l a te f o r m a t
< -|
<<
E sc W e ll sh a p e
< -|
'F l a t' 'R o u n d '
<<
Esc C r o s s w is e a s p
< -|
E sc C e n te r p o s
Esc S id e p o s f r o n t
< -|
< -|
If 'C r o s s -
E sc S id e p o s r e a r w is e a s p ' = N o
< -|
A s p ir a tio n H t
E sc
< -|
E sc D is p p o s
< -|
E sc B o tto m d is p h t
< -|
Esc O v f l o w d is p h t
< -|
S to r in g
Table 8.: Define Plate

For details please refer to chapter 9.5 Define Plate Menu

7.3.6 Setup
<< >>
S etu p
< -|
Esc La ng ua ge
< -|
<<
Esc Ma nifo ld
< -|
<<
E sc W a sh liqu id s
< -|
<<
Esc W a st full d et
< -|
R in se em p ty <<
Esc
d et
< -|
W a sh 1 e m pty << if 'W a sh

Esc
d et liq uids' = 1
< -|
W a sh 2 e m pty << if ' W a sh

Esc
d et liq uids' = 2
< -|
W a sh 3 e m pty <<
Esc
d et
< -|
<<
Esc L iq uid w arning
< -|
<<
Esc W aste w arning
< -|
<<
Esc P la te w arning
< -|
<<
Esc Ke y click
< -|
Sto ring
Table 9.: Setup

For details please refer to chapter 9.6 Setup Menu

7.3.7 Service
<< Service >>
<-|
Sensors Esc
+/-
Valves Esc
+/-
Pumps Esc
+/-
PlatInit Esc
+/-
PlatPos Esc
+/-
MFInit Esc
+/-
MFPos Esc
+/-
MFAdjust Esc
+/-
PlAdjust Esc
+/-
Eeprom Esc
+/-
DelParam Esc
Table 10.: Service

For details please refer to chapter 9.7 Service Menu

7.4 Description of the key functions
<F1> / <F2> - Function Keys: Used to scroll through the different
menus. Depending on the menu, other functions can be assigned
to these keys. The current function is always shown in the
display right above the key.
<+> / <-> - These keys are used for numerical or text input and to
select options from a list (e.g. selection of the procedure to be
started).
<enter> - confirms the text / numerical entry or selection and
proceeds to the next dialogue element.

8 Start-up and system setup
8.1 Start-Up
Place the device on a suitable working surface
Follow the steps described in the chapters 4.8 Installation

requirements, Environmental operating conditions and
4.9 Instrument installation procedure
Connect power cable to standard mains plug
Switch on main switch (rear left side). The Fluido 2

performs the complete initialization and shows the
main menu after approx. 10 seconds.Display
Run Procedure:
<< >> Proc1
Screen 11.: Display

Description:
The first line of the display shows the name of the menu or the
current function of a sub-menu.
The second line is split into 3 parts:
<< - current function assigned to the first function key
(e.g. previous menu, escape, ...)
>> - current function assigned to the second function key
(e.g. next menu, escape, ...)
Proc1 - current selection (or field for text or numerical
input); use the <-> and <+> keys to alter a selection or
change a value
8.2 Control of the system setup
Setup
<< >>
Screen 12.: Setup Menu
Please make sure to set all options according to your requirements
before programming new procedures, plates or cycles and before
running procedures. For details on this menu please refer to chapter
9.6 Setup Menu

9 Programming
9.1 Run Procedure
Run Procedure:
<< >> Proc1
Screen 13.: Run Procedure
In this menu you can select the procedure to be started. All
procedures defined in the "Define Procedure Menu" can be
selected.
Function keys:
<F1> or <F2>
Select another menu.
<+> or <->
Select a procedure to be started.
<enter>
Confirm the selection of the procedure and go to the next
screen.
Washliquid OK?
No Yes
Screen 14.: Washliquid Check

A warning to check the liquid level of the wash bottle is displayed
(this function needs to be activated in the "Setup Menu").
Function keys:
<F1> or <F2>
Select "Yes" or "No". If "No" is selected the instrument will
return to the main menu.
Waste empty?
No Yes
Screen 15.: Waste Check

A warning to 0check the liquid level of the waste bottle is
displayed (this function needs to be activated in the "Setup Menu").

Function keys:
<F1> or <F2>
Warning: If "Yes" is selected without a sufficient volume for waste liquid in the
waste bottle the instrument can be damaged!
Plate inserted?
No Yes
Screen 16.: Plate Check

A warning to check whether a plate is inserted is displayed (this
function needs to be activated in the "Setup Menu").
Function keys:
<F1> or <F2>
Warning: If "Yes" is selected without a plate inserted to the instrument spillage
will be caused which can damage the instrument and/or be harmful to
the ambient (depending on the used wash liquid)!
Nr of stripes:
Esc 12
Screen 17.: Nr of stripes check
The display prompts you to enter the right numbers of stripes (this
function needs to be activated in the "Setup Menu").
Function keys:
<F1>
Select Esc: the instrument will return to the main menu
<->/<+>
Select number of stripes
<enter>
Confirms the selection and starts procedure
After confirmation of the above warnings the selected procedure

will be started. During the run the display will show the currently
performed cycles.

9.2 Prime/Rinse Menu
Prime/rinse:
<< >> Manual
Screen 18.: Prime/rinse Menu
This menu allows to start a manual, automatic or periodic prime /
rinse cycle(s).
Function keys:
<F1> or <F2>
<+> or <->
Select the desired mode for prime / rinse. Available options are:
manual, automatic, periodic
<enter>
Confirm the selection of the prime / rinse mode and go to the
next screen.
9.2.1 Manual Prime/Rinse
Liquid:
Esc Wash 1
Screen 19.: Liquid Selection
Select the liquid to be used for prime / rinse.
Function keys:
<F1> or <F2>
Select "Esc" to return to the main menu.
<+> or <->
Select the desired liquid.
<enter>
Confirm the selection of the liquid and go to the next screen.
Manual pri/rinse
Esc Go
Screen 20.:Manual Prime/rinse
The prime / rinse can be started with the corresponding function
key ("Go") and will be performed as long as this key is pressed.
Function keys:
<F1> or <F2>
Select "esc" to return to the main menu or "Go" to start the
prime / rinse (keep this key pressed as long as you like the
prime / rinse to be performed).
9.2.2 Automatic Prime/Rinse
Liquid:
Esc Wash 1
Function keys:
<F1> or <F2>
<+> or <->
<enter>
Volume:
Esc 50 ml
Screen 22.: Volume Selection

Select the liquid volume for prime / rinse.

Function keys:
<F1> or <F2>
<+> or <->
Select the desired volume in 1ml steps (max. 200ml).
<enter>
Confirm the selection of the liquid volume and go to the next
screen.
Start Pri/Rinse
Esc
Screen 23.: Start Prime / Rinse
Function keys:
<F1> or <F2>
<enter>
Press <enter> to start the prime / rinse cycle with the above set
parameters.
P/R in process
Stop
Screen 24.: Status Message
Function keys:
<F1> or <F2>
Select "Stop" to cancel the current prime / rinse cycle and return
to the main menu.
9.2.3 Periodic Prime/Rinse
Liquid:
Esc Wash 1

Function keys:
<F1> or <F2>
<+> or <->
<enter>
Volume:
Esc 50 ml
Screen 26.: Volume Selection

Select the liquid volume for periodic prime / rinse.
Function keys:
<F1> or <F2>
<+> or <->
Select the desired volume in 1ml steps (max. 200ml).
<enter>
Confirm the selection of the liquid volume and go to the next
screen.
Rinse Period:
esc 10 min
Screen 27.: Rinse Period Selection

Select the period of time after which the instrument is rinsed.
Function keys:
<F1> or <F2>
<+> or <->
Select the desired time period in steps of 1min (max. 200min).
<enter>
Confirm the selection of the time and go to the next screen.

Start Pri/Rinse
esc
Screen 28.: Start Prime / Rinse

Function keys:
<F1> or <F2>
<enter>
Press <enter> to start the prime / rinse cycle with the above set
parameters.
Periodic rinse
Stop
Screen 29.: Status Message

Function keys:
<F1> or <F2>
Select "Stop" to cancel the current prime / rinse cycle and return
to the main menu.
9.3 Define Procedure Menu
Def Procedure:
<< >> <new>
Screen 30.: Define Procedure Menu
In this menu a procedure can be defined. Every procedure contains
one or more cycles and a plate. The cycles and the plate must be
defined prior to the procedure.
Function keys:
<F1> or <F2>
<+> or <->
Select "<new>" to define a new procedure or select an existing
procedure to edit it.
<enter>
Confirm the selection of the procedure and go to the next
screen.

Name:
Esc Chg 1
Screen 31.: Procedure Name Entry

The name of the procedure may be changed.
Function keys:
<F1> or <F2>
Select "Chg" to change the name for the procedure.
Name:
Esc sel A_
Screen 32.: Procedure Name Entry

Function keys:
<F1> or <F2>
Select "Esc" to return to the main menu without saving any
changes or "Sel" to select the current letter/number.
<+> or <->
Use these keys to scroll through the available letters (A-Z) and
numbers (0-9)
<enter>
Confirm the name of the procedure and go to the next screen.
Liquid:
<< Esc Wash 1
Screen 33.: Wash Liquid Selection

Select the wash liquid to be used for this procedure.
Function keys:
<F1> or <F2>
changes or "<<" to return to the previous screen.
<+> or <->
Use these keys to enter the wash liquid.
<enter>
Confirm the wash liquid and go to the next screen.

Cycle1:
<< Esc C1
Screen 34.: Cycle1 Selection

Select the first cycle to be used for this procedure. The cycles must
be defined in the "Define Cycle Menu" prior to the definition of the
procedure.
Function keys:
<F1> or <F2>
<+> or <->
Use these keys to scroll through the available cycles.
<enter>
Confirm the selection of the first cycle and go to the next
screen.
Cycle2:
<< Esc ---
Screen 35.: Cycle2-8 Selection

Select the cycles 2 to 8 to be used for this procedure. The cycles
must be defined in the "Define Cycle Menu" prior to the definition
of the procedure. By selecting “---“ the previous cycle will be the
last cycle in the procedure.
Function keys:
<F1> or <F2>
<+> or <->
Use these keys to scroll through the available cycles.
<enter>
Confirm the selection of the cycle and go to the next screen.
Final asp time:

<< Esc 0 sec
Screen 36.: Final Aspiration Time Entry

Select the duration of the final aspiration.
Function keys:
<F1> or <F2>
<+> or <->
Use these keys to enter the time for the final aspiration in steps
of 1sec (max. 10 seconds).
<enter>
Confirm the final aspiration time and go to the next screen.
Final asp speed:

<< Esc High
Screen 37.: Final Aspiration Power Entry

Select the power for the final aspiration.
Function keys:
<F1> or <F2>
<+> or <->
Use these keys to enter the power for the final aspiration
(“Low”, Medium”, “High”).
<enter>
Confirm the final aspiration power and go to the next screen.
Processing mode:
<< Esc Plate
Screen 38.: Processing Mode

Select the processing mode to be used for this procedure.
Function keys:
<F1> or <F2>
<+> or <->
Use these keys to scroll through the available modes (“Plate” or
“Strip”).
<enter>
Confirm the selection of the processing mode and go to the next
screen.
Plate:
<< Esc FB1
Screen 39.: Plate Selection

Select the plate to be used for this procedure. The plate must be
defined in the "Define Plate Menu" prior to the definition of the
procedure.
Function keys:
<F1> or <F2>
<+> or <->
Use these keys to scroll through the available plates.
<enter>
Confirm the selection of the plate and go to the next screen.
Ask strip nr:

<< Esc No
Screen 40.: Ask for Last Strip

Select "Yes" if the selection of a "number of strips" should be
possible every time this procedure is started. This can be useful if
for example full plates and plates with just a few strips are used
subsequently with the same procedure.
Function keys:
<F1> or <F2>
<+> or <->
Use these keys to toggle between "Yes" and "No". If "Yes" is
selected you can define the last strip every time the procedure is
started. "No" means that every time all strips of a plate are
washed.
<enter>
Confirm the selection and go to the next screen.
Delay final asp:

<< Esc Yes
Screen 41.: Delay of Final Aspiration

Select "Yes" to delay the final aspiration of the plate. This is used
in strip mode: The final aspiration is performed only after the last
cycle in the last filled strip is done and the soak time has expired.
Function keys:
<F1> or <F2>
<+> or <->
Use these keys to toggle between "Yes" and "No". If "Yes" is
selected the final aspiration is performed after the last cycle for
all strips is finished.

<enter>
Confirm the selection. The set parameters will be stored and the
program returns to the main menu.
9.4 Define Cycle Menu
Def Cycle:
<< >> <new>
Screen 42.: Define Cycle Menu
This menu allows to define all parameters of a cycle. A cycle is
required to define a complete wash-procedure.
Function keys:
<F1> or <F2>
<+> or <->
Select "<new>" to define a new cycle or select an existing cycle
to edit it.
<enter>
Confirm the selection of the cycle and go to the next screen.
Name:
Esc Chg 1
Screen 43.: Cycle Name Entry

The name of the cycle may be changed.
Function keys:
<F1> or <F2>
Select "Chg" to change the name for the cycle.
Name:
Esc Sel A_
Screen 44.: Cycle Name Entry

Function keys:
<F1> or <F2>
<+> or <->
numbers (0-9)
<enter>
Confirm the name of the cycle and go to the next screen.
Aspiration time:
<< Esc 5 sec
Screen 45.: Aspiration Time Entry

Function keys:
<F1> or <F2>
<+> or <->
Use these keys to change the aspiration time (the time the
manifold remains inside a well during aspiration) in steps of
1sec (range: 0 - 10 seconds).
<enter>
Confirm the selection of the aspiration time and go to the next
screen.
Aspir power:
<< Esc High
Screen 46.: Aspiration Power Entry

Function keys:
<F1> or <F2>
<+> or <->
Use these keys to change the power with which the liquid is
aspirated off a well (“Low”, “Medium”, “High”).
<enter>
Confirm the selection of the aspiration power and go to the next
screen.
Disp volume:
<< Esc 1000 µl
Screen 47.:Dispense Volume Entry

Function keys:
<F1> or <F2>
<+> or <->
Use these keys to change the dispense volume for this cycle in
steps of 50µl (range 0 - 2000µl).

<enter>
Confirm the selection of the volume and go to the next screen.
Disp power:
<< Esc High
Screen 48.: Dispenser Power Selection

Function keys:
<F1> or <F2>
<+> or <->
Use these keys to change the power (pressure) of the liquid for
dispensing (“Low”, “Medium”, “High”).
<enter>
Confirm the selection of the dispensing power and go to the
next screen.
Wash mode:
<< Esc Overflow
Screen 49.: Wash Mode Selection

Two different wash modes are available:
Bottom: the needles of the manifold are placed into the well (to the
defined position for the used plate) during the wash procedure.
This means that basically only the bottom of the well is washed.
Overflow: the needles of the manifold are placed to a certain
position above the well (to be also defined in the "Define Plate
Menu"). Therefore the whole well is thoroughly washed as the
wash solution is only aspirated after it fills the well up to the
defined position and reaches the aspiration needles of the
manifold.
Function keys:
<F1> or <F2>
<+> or <->
Use these keys to toggle between "Bottom" and "Overflow".
Shaking time:
<< Esc 5 sec
Screen 50.: Shaking Time Entry

Function keys:
<F1> or <F2>
<+> or <->
Use these keys to change to shaking time in steps of 1sec
(range: 0 - 10 seconds).
<enter>
Confirm the selection of the shaking time and go to the next
screen.
Shaking intens:
<< Esc Medium
Screen 51.: Shaking Intensity Entry

Function keys:
<F1> or <F2>
<+> or <->
Use these keys to change the intensity for shaking (“Low”,
“Medium”, “High”).
<enter>
Confirm the selection of the shaking intensity and go to the next
screen.
Min soak time:

<< Esc 10 sec
Screen 52.: Minimum Soaking Time Entry

The minimum soaking time defines the time which must elapse at
least between dispensing and aspirating a well.
Function keys:
<F1> or <F2>
Select "esc" to return to the main menu without saving any
<+> or <->
Use these keys to change the minimum soaking time in steps of
10sec (range: 0 - 1000 seconds).
<enter>
Confirm the selection of the minimum soaking time. The set
parameters will be stored and the program returns to the main
menu.

9.5 Define Plate Menu
The following drawings help to find the correct positions of the

manifold and plate. Please note that curved bottom plates do not
allow crosswise aspiration!
Dispense needle Aspiration needle Dispense needle Aspiration needle
Vertical 0-Position Vertical 0-Position
Aspiration Aspiration
ht ht
Center pos
Side pos rear Side pos front
Horizontal 0-Position
Horizontal 0-Position
Drawing 53.: Aspiration Positions for flat bottom or curved bottom plates
Dispense needle Aspiration needle Aspiration needle
Vertical 0-Position Vertical 0-Position
Ovflow
disp ht
Bottom
disp ht
Horizontal 0-Position Disp pos Horizontal 0-position Disp pos
Drawing 54.: Wash Positions for flat bottom and curved bottom plates

Def Plate:
<< >> <new>
Screen 55.: Define Plate Menu

This menu allows to define all parameters for a plate. A defined
plate is required to for a complete wash-procedure.
Function keys:
<F1> or <F2>
<+> or <->
Select "<new>" to define a new plate or select an existing plate
to edit it.
<enter>
Confirm the selection of the plate and go to the next screen.
Name:
Esc Chg 1
Screen 56.: Plate Name Entry

The name of the plate may be changed.
Function keys:
<F1> or <F2>
Select “Esc” to return to main menu without saving any
changes
Select "Chg" to change the name for the plate.
<enter>
Confirm the existing name and go to the next menu
Name:
Esc Sel A_
Screen 57.: Plate Name Entry

Function keys:
<F1> or <F2>
<+> or <->
numbers (0-9)
<enter>
Confirm the name of the plate and go to the next screen.

Plate format:
<< Esc 8-strip
Screen 58.: Plate Format Selection

Function keys:
<F1> or <F2>
<+> or <->
Use these keys to toggle between 8-strip, 12-strip and 16-strip
plate.
<enter>
Confirm the selection of plate format and go to the next screen.
Well shape:
<< Esc Flat
Screen 59.:Well Shape Selection

Function keys:
<F1> or <F2>
<+> or <->
Use these keys to toggle between flat- and round-bottom plates.
<enter>
Confirm the selection of well shape and go to the next screen.
Center pos:
Esc Mov 0.1 mm
Screen 60.: Center Position Entry

This menu appears only if a round-bottom plate is selected. For
round bottom plates it is recommended to position the aspiration
needle in the center of the well. The center position may be defined
by "teach-in" (move the plate to the correct position with the <+>
and <-> keys).

Function keys:
<F1> or <F2>
changes.
Select “Mov” to position the plate to the current center position
and switch to the “teach-in” mode.
<+> or <->
Use these keys to enter the center position The plate can be
moved in steps of 0.1mm (range: -5.0 – 5.0mm).
<enter>
Confirm the center position and go to the next screen.
Center pos:
↓ Mov 0.1 mm
Screen 61.: Center Position “Teach-in” mode

In this ‘teach-in’ mode the round-bottom plate can be moved to the
correct center position by using <+> or <->.
Function keys:
<F1> or <F2>
Select "↓" to automatically detect the bottom of the well
(changes to “↑”).
Select "↑" to move the manifold up.
Select “Mov” to move the plate transport to the current center
position.
<+> or <->
Use these keys to enter the center position. The plate can be
<enter>
Confirm the center position and go to the next screen.
Crosswise aspir:
<< Esc Yes
Screen 62.: Crosswise Aspiration Selection

If a flat-bottom plate is selected this menu appears. When using
crosswise aspiration the aspiration needle is first moved close to
one wall of the well (side pos front) and aspirates there. After the
defined aspiration time the needle is moved up to avoid scratching
the bottom and is shifted to the opposite wall (side pos rear). There
the needle is moved down and another aspiration sequence takes
place. For a better aspiration result crosswise aspiration is
advisable. Crosswise aspiration can only be used for flat-bottom
plates.

Function keys:
<F1> or <F2>
<+> or <->
Use these keys to toggle between "Yes" and "No".
<enter>
Confirm the selection for crosswise aspiration and go to the
next screen.
Side pos front:

Esc Mov 0.1 mm
Screen 63.: Side Position Front Entry

In this menu the side position front for a flat-bottom plate is
defined. If you choose crosswise aspiration this is the first
aspiration position. Else this is the aspiration position: for a better
aspiration result it is recommended to position the needle close to
one wall. The side position front may be defined by "teach-in"
(move the plate to the correct position with the <+> and <-> keys).
Function keys:
<F1> or <F2>
changes.
Select “Mov” to position the plate to the current side position
front and switch to the “teach-in” mode.<+> or <->
Use these keys to enter the side position front. The plate can be
<enter>
Confirm the side position front and go to the next screen.
Side pos front:

↓ Mov 0.1 mm
Screen 64.: Side Position Front “Teach-in” mode

In this "teach-in" you can move the plate to the correct position
with the <+> and <-> keys.
Function keys:
<F1> or <F2>
Select “Mov” to move the plate transport to the current side
position front.

<+> or <->
Use these keys to enter the side position front. The plate can be
<enter>
Confirm the side position front and go to the next screen.
Side pos rear:

Esc Mov 0.1 mm
Screen 65.: Side Position Rear Entry

In this menu the side position rear for a flat-bottom plate is defined.
It is only available if crosswise aspiration is enabled and it defines
the second aspiration position. The side position rear may be
defined by "teach-in" (move the plate to the correct position with
the <+> and <-> keys).
Function keys:
<F1> or <F2>
changes.
Select “Mov” to position the plate to the current side position
rear and switch to the “teach-in” mode.
<+> or <->
Use these keys to enter the side position rear. The plate can be
<enter>
Confirm the side position rear and go to the next screen.
Side pos rear:

↓ Mov 0.1 mm
Screen 66.: Side Position Rear “Teach-in” mode

In this "teach-in" mode the plate may be moved to the correct
position with the <+> and <-> keys.
Function keys:
<F1> or <F2>
Select “Mov” to move the plate transport to the current side
position rear.

<+> or <->
Use these keys to enter the side position rear. The plate can be
<enter>
Confirm the side position rear and go to the next screen.
Aspiration ht:
Esc Mov 7.0 mm
Screen 67.: Aspiration Height Position

The aspiration height position defines the height of the manifold
during aspirating a well. It may be defined by "teach-in" (move the
manifold to the correct position with the <+> and <-> keys).
Function keys:
<F1> or <F2>
changes.
Select “Mov” to position the manifold to the current aspiration
height and switch to the “teach-in” mode.
<+> or <->
Use these keys to enter the aspiration height in steps of ~0.1mm
(range: 0 – 18.8mm).
<enter>
Confirm the aspiration height and go to the next screen.
Aspiration ht:
↓ 0.1 mm
Screen 68.: Aspiration Height “Teach-in” mode

In this special "teach-in" mode the manifold can be moved to the
correct height with the <+> and <-> keys. This mode features an
automatic bottom detection. For an optimal aspiration result move
the aspiration needle about 0.5mm up.
Function keys:
<F1> or <F2>
Select "↓" to automatically detect the bottom of the well.
<+> or <->
Use these keys to move the manifold. The manifold can be
moved in steps of ~0.1mm (range: 0 – 18.8mm).
<enter>
Confirm the aspiration height and go to the next screen.

Disp pos:
Esc Mov 1.6 mm
Screen 69.: Dispense Position

For washing the dispensing needle is placed above the well. From
there it dispenses the washing solution into the well. The aspiration
needle aspirates an overflow and prevents liquid spilling on next
wells.
The dispense position defines the position of the plate below the
manifold during washing. The dispensing needle should be above
the well and the aspiration needle within. It may be defined by
"teach-in" (move the plate to the correct position with the <+> and
<-> keys).
Function keys:
<F1> or <F2>
changes.
Select “Mov” to position the plate to the current dispense
position and switch to the “teach-in” mode.
<+> or <->
Use these keys to enter the dispense position in steps of 0.1mm
(range: -5.0 – 5.0mm).
<enter>
Confirm the dispense position and go to the next screen.
Disp pos:
↓ Mov 0.1 mm
Screen 70.: Dispense Position “Teach-in’ mode

In this "teach-in" mode the plate can be moved to the correct
position with the <+> and <-> keys. With “Mov” and “↓” check if
the needles are in correct position.
Function keys:
<F1> or <F2>
Select “Mov” to move the plate transport to the current dispense
position.
<+> or <->
Use these keys to enter the dispense position. The plate can be
<enter>
Confirm the dispense position and go to the next screen.

Bottom disp ht:
Esc Mov 0.0mm
Screen 71.: Bottom Dispensing Position

For additional bottom washing the needles are moved upwards to
Bottom dispensing height and then down again to the aspiration
height.
The bottom dispensing height defines the vertical position of the
manifold during dispensing of a bottom wash cycle. The dispensing
height must be higher or equal the aspiration height. It may be
defined by "teach-in" (move the manifold to the correct position
with the <+> and <-> keys).
Function keys:
<F1> or <F2>
changes.
Select “Mov” to position the manifold to the current bottom
dispense height and switch to the “teach-in” mode.
<+> or <->
Use these keys to enter the dispense height in steps of ~0.1mm
(range: 0 – 18.8mm).
<enter>
Confirm the bottom dispensing position and go to the next
screen.
Bottom disp ht:

↓ 0.1 mm
Screen 72.: Bottom Dispense Height “Teach-in” mode

In this "teach-in" mode you can move the manifold to the correct
height with the <+> and <-> keys.
Function keys:
<F1> or <F2>
<+> or <->
<enter>
Confirm the bottom dispense height and go to the next screen.

Ovflow disp ht:
Esc Mov 4.0mm
Screen 73.: Overflow Dispensing Height

It is recommended to use a dispense volume slightly higher than
the well volume to produce an overflow phase. The slight flow rate
variations among dispensing needles are compensated by the
overflow phase.
The overflow dispensing height defines the height of the manifold
for overflow washing. The position must be higher or equal as the
aspiration height. It may be defined by "teach-in" (move the
manifold to the correct position with the <+> and <-> keys).
Function keys:
<F1> or <F2>
changes.
Select “Mov” to position the manifold to the current overflow
dispense height and switch to the “teach-in” mode.
<+> or <->
Use these keys to enter the desired overflow dispense height in
steps of ~0.1mm (range: 0 – 18.8mm).
<enter>
Confirm the overflow dispensing height and go to the next
screen.
Ovflow disp ht:

↓ 0.1 mm
Screen 74.: Overflow Dispense Height “Teach-in’ mode

In this "teach-in" mode you can move the manifold to the correct
height with the <+> and <-> keys.
Function keys:
<F1> or <F2>
<+> or <->
<enter>
Confirm the overflow dispense height. The set parameters will
be stored and the program returns to the main menu.

9.6 Setup Menu
Please make sure to set all options according to your requirements.
Setup
<< >>
Screen 75.: Setup Menu
Use the <F1> / <F2> keys to select the setup menu. Confirm the
selection with <enter>.
Language:
Esc English
Screen 76.: Language Setup
Use the <-> / <+> keys to select the language and confirm with
<enter>.
Manifold:
<< Esc 8needle
Screen 77.: Manifold Setup
Use the <-> / <+> keys to select the manifold and confirm with
<enter>.
Wash liquids:
<< Esc 2
Screen 78.:Liquids Setup
Use the <-> / <+> keys to select the number of wash liquids
connected to your instrument and confirm with <enter>. Depending
on the hardware configuration of your instrument you can use up to
3 wash liquids .

Waste full det:
<< Esc Off
Screen 79.: Waste Setup
Use the <-> / <+> keys to select whether a liquid level sensor is
attached to the waste bottle and confirm with <enter>. To use this
function a liquid level detector must be connected to the instrument
and the waste bottle.
If activated the liquid level of the waste bottle will be checked
before the procedure is started. In case that the level of the liquid in
the waste bottle is above the sensor a corresponding warning will
be displayed.
Rinse empty det:

<< Esc Off
Screen 80.:Rinse Setup
attached to the rinse bottle and confirm with <enter>. To use this
and the rinse bottle.
If activated the liquid level of the rinse bottle for a started
procedure will be checked before the procedure is started. In case
that the level of the liquid in the rinse bottle is below the sensor a
corresponding warning will be displayed.
Wash1 empty det:

<< Esc Off
Screen 81.:Wash1 Setup
attached to the wash1 bottle and confirm with <enter>. To use this
and the wash1 bottle.
If activated the liquid level of the wash1 bottle for a started
that the level of the liquid in the wash1 bottle is below the sensor a
Wash2 empty det:

<< Esc Off
Wash3 empty det:

<< Esc Off
Liquid warning:
<< Esc On
Screen 84.: Liquid Warning Setup
Use the <-> / <+> keys to activate/deactivate the liquid warning
and confirm with <enter>. If activated a reminder to check the level
of the wash bottle(s) is displayed prior to the start of a procedure.
Waste warning:
<< Esc On Yes
Screen 85.: Waste Warning Setup

Use the <-> / <+> keys to activate/deactivate the waste warning
and confirm with <enter>. If activated a reminder to check the level
of the waste bottle is displayed prior to the start of a procedure.
Plate warning:
<< Esc On Yes
Screen 86.: Plate Warning Setup
Use the <-> / <+> keys to activate/deactivate the plate warning and
confirm with <enter>.
Keyclick:
<< Esc On
Screen 87.:Keyclick Setup
Use the <-> / <+> keys to activate/deactivate the keyclick and
confirm with <enter>.
Emergencystop:?
<< Esc On
Screen 88.: Emergencystop Setup
Use the <-> /<+> keys to activate/deactivate the emergencystop
and confirm with <enter>
If a strip of a microplate or the microplate itself is not inserted
correctly the tips of the manifold will hit the bottom. This triggers
the bottom detection sensor and every process will be stopped
immediately.
9.7 Service Menu
Service
<< >> Sensors
Screen 89.: Service Menu
This menu allows to check various functions of the instrument. It is only intended to be
used by trained service personnel.

10 ERROR MESSAGES
10.1.1 Duplicate Name
Duplicate name
Press any key
Screen 90.: Duplicate Name
The name for the procedure, cycle or plate is already used
Function keys:
<enter>
Confirm the message.
10.1.2 Wrong Manifold
Wrong manifold
Press any key
Screen 91.: Wrong Manifold
The wrong manifold for the selected procedure is installed.
Function keys:
<enter>
10.1.3 No Cycle Defined
No cycle defined
Press any key
Screen 92.: No Cycle Defined
There is no cycle defined.
Function keys:
<enter>
10.1.4 No Plate Defined
No plate defined
Press any key
Screen 93.: No Plate Defined
There is no plate defined.
Function keys:
<enter>
10.1.5 Waste Full
Waste full
Press any key
Screen 94.: Waste Full
The waste is full. Empty the waste bottle.
Function keys:
<enter>
10.1.6 Liquid Empty
Liquid empty
Press any key
Screen 95.: Liquid Empty
The selected wash liquid is empty.
Function keys:
<enter>

10.1.7 Stop Pressed
Stop pressed
Press any key
Screen 96.: Stop Pressed
The user has stopped the instrument.
Function keys:
<enter>
10.1.8 Plate Transport Error
Platetrans error
Press any key
Screen 97.: Plate Transport Error
The plate transport failed.
Function keys:
<enter>
10.1.9 Manifold Transport Error
MF-trans error
Press any key
Screen 98.:Manifold Transport Error
The manifold transport failed.
Function keys:
<enter>

11 Maintenance
11.1 Recommended regular inspection
The Fluido 2 is designed in a way that during appropriate use no

danger for the user is caused.
Correct functional performance is assured by an automatic check of
the relevant technical parameters during operation. In case of a
problem the instrument reports an error message.
Anthos recommends to have the instrument inspected by an
authorized service partner at an interval of about 2 years. During
this inspection the correct function of the instrument can be
verified.
11.2 Approved parts
Except for the parts shown in the following list only parts supplied
by Anthos or an authorized Anthos Distributor may be installed in
or used with Anthos Fluido 2:
Fuses: as specified in chapter
4.1.1 Rated operating conditions
Power cable: CE-marked power cable with connected protective
earth and protective earth connector
(see also 4.8 Installation requirements, Environmental
operating conditions)
External Computer controlling the instrument
(see also 4.9.1 Connecting the instrument with an external
computer)
11.3 Exchanging a Fuse
In case of malfunction (the green stand-by indicator is not

illuminated), the fuses located in the mains inlet next to the mains
socket on the rear side of the device can be checked and replaced if
necessary.
• Disconnect the instrument from mains by unplugging the power
cable.
• Open fuse-carrier next to the mains socket with a screwdriver
• Remove fuse
• Insert spare fuse included in supply (2 pcs. T 2.5 A, 250V,
• Close fuse-carrier.
• Turn device on and check function. In case of malfunction, call
a service technician.

11.4 Cleaning the instrument
This instrument is a precision instrument and the requires regular

Liquid spillage
Regular cleaning
cloth.
Fittings and tubing
The usual wash buffer tends to crystallize easily on air and can
block tubing and fittings then. Use the automatic rinse program
daily and do not leave the tubing filled with wash buffer for an
extended period of time.
11.5 Disinfection Procedure
If the laboratory has no specific disinfection procedure, the

following procedure should be used to disinfect the instrument.
The instrument should be disinfected using a suitable disinfection
solution.
1. Disconnect the instrument from the mains power supply.
2. Carefully wipe all the outside surfaces of the instrument and the
plate area with a wad of cotton wool that has been soaked in the
disinfection solution.
Note: Ensure that disposable gloves are worn.

3 Place the instrument in to a large plastic bag.
4. Place a wad of cotton wool that has been soaked in the
disinfection solution in to the plastic bag.
Note: Ensure that the wad is not touching the instrument.
5. Close and seal the plastic bag.
6. Leave the instrument to stand in the plastic bag for at least 24
hours.
7. After the standing time, remove the instrument from the plastic
bag and wipe all the outside surfaces of the instrument and the
plate support area with a wad of cotton wool which has been
soaked in a 50% Alcohol solution.
8. Repeat the disinfection procedure on any accessories which are
also being moved or returned.
The pumps can be disinfected by dispensing and aspirating a
sufficient amount of disinfectant fluid. After the disinfection
procedure dispense > 200 ml of distilled water to remove all
disinfectant fluid from the liquid carrying parts.

12 Intended Use
This device is intended to be used to remove non-bound sample-

sera or to remove reagents from 96-well respectively 384-well
microplates or format-identical strips. By means of repeated liquid
exchange down to a defined minimum rest volume, for which the
sample-/reagent-liquid is aspirated from the wells into a "waste"-
bottle and the wash liquid is dispensed from stock-("wash-")bottles
into the wells of the microplate, the concentration of the sample
shall be diluted until no diagnostically-relevant rest remains.
The main field of use is ELISA-testing operated and carried out by
trained specialist stuff (medical and/or laboratory).
The Fluido 2 microplate washer has been designed to meet the
98/79/EC directive of the EU for in-vitro diagnostic devices.
12.1 Quality control
The quality control criteria of a diagnostic kit have to be described

in the package insert of the kit. For the performance of any reagent
quality control it has to be referred exclusively to those
instructions.
It is the user's responsibility to ensure proper operation according to the
instructions given with each individual test!

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Fluido 2

Tel.: +44/1223 423723

June 2008 Fluido 2 Page 1

June 2008 Fluido 2 Page 3

The Fluido 2 is a fully automated, programmable 96-well

June 2008 Fluido 2 Page 4

June 2008 Fluido 2 Page 5

2.1.1 Safety symbols

In Vitro Diagnostic Device

Protective ground terminal

Caution, see enclosed document

June 2008 Fluido 2 Page 6

3.1 Cleaning the instrument

This instrument is a precision instrument and it requires regular

June 2008 Fluido 2 Page 7

4.1 Metrological specification

4.1.1 Rated operating conditions

Warming-up time: Ready for operation immediately

4.1.1 Limits of error and tolerance limits

Dispense volume: wash liquid volume adjustable

4.2 General technical data

Weight: 6,2 kg net

4.2.2 Power supply

Consumption: 80VA (maximum)

June 2008 Fluido 2 Page 8

Serial interface (RS232 9-pin male connector) for remote control

liquid level sensors for wash and waste bottles

This instrument is a precision instrument and it requires regular

4.4 General features

The Fluido 2 incorporates state of the art technology and software

4.5 Standard delivery content

4.6 Warnings and directions

If inflammable, toxic or biologically hazardous substances are used

June 2008 Fluido 2 Page 11

June 2008 Fluido 2 Page 12

1. Visually inspect, the container for damage, before opening it.

4.8 Installation requirements, Environmental operating

Flat, dry, clean and vibration-proof working area (50 cm x 35

June 2008 Fluido 2 Page 13

The following procedures detail the necessary steps to be followed

June 2008 Fluido 2 Page 14

The stand-by indicator may show 3 different states:

4.9.1 Connecting the instrument with an external computer

Turn the instrument off.

June 2008 Fluido 2 Page 15

June 2008 Fluido 2 Page 16

This chapter gives the description of the main components of the

5.2 Main components

5.3 Hydraulic system

5.3.1 Dispensing system

The dispensing system consists of the input selection valves, the

5.3.2 Aspirating system

The aspiration is performed by a liquid pump connected to the

June 2008 Fluido 2 Page 17

June 2008 Fluido 2 Page 18

Warranty shall lose effect if:

In original condition the instrument meets all safety regulations for

June 2008 Fluido 2 Page 19

7.1 Software Navigation