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    ISP5 (Glycerol Asparagine Medium)

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    zemoura

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    ISP5 (Glycerol Asparagine Medium)

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    zemoura
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    40 views2 pages

    ISP5 (Glycerol Asparagine Medium)

    Uploaded by

    zemoura

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    © All Rights Reserved
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    of 2

    ISP Medium No.

    5 (Glycerol Asparagine Agar Base) M360


    Intended Use:
    Recommended for cultivation of Streptomyces species as per International Streptomyces Project.
    Composition**
    Ingredients Gms / Litre
    L-Asparagine 1.000
    Dipotassium hydrogen phosphate 1.000
    *Trace salt solution (ml) 1.000
    Agar 20.000
    1ml of Trace salt solution contains -
    Ferrous sulphate heptahydrate 0.001
    Manganese chloride tetrahydrate 0.001
    Zinc sulphate heptahydrate 0.001
    Final pH ( at 25°C) 7.4±0.2
    **Formula adjusted, standardized to suit performance parameters
    Directions
    Suspend 22.002 grams in 1000 ml purified/distilled water containing 10 ml glycerol. Heat to boiling to dissolve the
    medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri
    plates.

    Principle And Interpretation


    ISP Medium No. 5 (Glycerol Asparagine Agar Base) is based on the formulation described by Shirling and Gottlieb (4)
    and is used for cultivation and characterization of Streptomyces species as recommended by the International
    Streptomyces Project. Being primarily soil inhabitants, Streptomyces are most commonly limited to causing
    actinomycotic mycetoma. Areas more prone to formation of mycetomas are those that are frequently traumatized or that
    come into contact with soil (2).
    This medium provides consistent and reproducible characteristic features of Streptomyces. Glycerol serves as the
    carbon source while asparagine is the amino acid source for the growth of Streptomyces species. Trace mineral
    requirement of Streptomyces is satisfied by the trace salt solution, which contains various salts. Dipotassium
    phosphate buffers the medium.
    Type of specimen
    Food samples

    Specimen Collection and Handling:


    For food samples, follow appropriate techniques for sample collection and processing as per guidelines (3). After use,
    contaminated materials must be sterilized by autoclaving before discarding.

    Warning and Precautions :


    Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection.
    Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established
    guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.

    Limitations :
    1. Further biochemical tests must be carried out for confirmation.
    Performance and Evaluation
    Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at
    recommended temperature.

    Quality Control
    Appearance
    Off-white to yellow homogeneous free flowing powder

    Please refer disclaimer Overleaf.


    HiMedia Laboratories Technical Data

    Gelling
    Firm, comparable with 2.0% agar gel.
    Colour and Clarity of prepared medium
    Light amber coloured, clear to slightly opalescent gel forms in Petri plates
    Reaction
    Reaction of 2.2% w/v aqueous solution containing 1.0% glycerol at 25°C. pH : 7.4±0.2
    pH
    7.20-7.60
    Cultural Response
    Cultural characteristics observed after an incubation at 25-30°C upto 15 days.
    Organism Growth

    Streptomyces albus subsp good-luxuriant


    albus ATCC 3006
    Streptomyces lavendulae good-luxuriant
    ATCC 8664
    Streptomyces peucetius good-luxuriant
    ATCC 29050

    Storage and Shelf Life


    Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the
    label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump
    formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation.
    Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly
    after use. Product performance is best if used within stated expiry period.

    Disposal
    User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow
    established laboratory procedures in disposing of infectious materials and material that comes into contact with sample
    must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).

    Reference
    1. Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
    2. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)
    Manual of Clinical Microbiology, 11th Edition. Vol. 1.
    3. Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination of
    Foods, American Public Health Association, Washington, D.C.
    4. Shirling E. B. and Gottlieb D., 1966, International J. Systemic Bacteriol., 16:3.

    Revision : 02 / 2021

    Disclaimer :
    User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in this and other
    related HiMedia™ publications. The information contained in this publication is based on our research and development work and is to the best of our
    knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to specifications and information related to the
    products at any time. Products are not intended for human or animal or therapeutic use but for laboratory,diagnostic, research or further manufacturing
    use only, unless otherwise specified. Statements contained herein should not be considered as a warranty of any kind, expressed or implied, and no
    liability is accepted for infringement of any patents.
    HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6116 9797 Corporate office :
    A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: [email protected]
    Website: www.himedialabs.com

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