MJARD - Volume 43 - Issue 3 - Pages 345-360
MJARD - Volume 43 - Issue 3 - Pages 345-360
MJARD - Volume 43 - Issue 3 - Pages 345-360
ABSTRACT
The current work was carried out at the Nursery of Ornamental Plants,
Faculty of Agriculture, Minia University, during the two growing seasons of
2021/2022 and 2022/2023, to examine the impact of compost levels (0, 250,
500 and 750 g/pot), chitosan (20 and 40 ppm), thiamine (25 and 50 ppm)
treatments and their interactions on vegetative and flowering traits and some
chemical constituents of Calendula officinalis, L. plant.
Data proved that all tested vegetative characters (plant height, number of
main branches/plant, plant dry weight) and flowering measurements (total
number of flowers/plant and total fresh and dry weights of flowers/plant), in
addition to some chemical composition like photosynthetic pigments content
in fresh leaves and nitrogen, phosphorus and potassium% in dry leaves were
significantly increased with rising compost level facing the control. The
treatment of 750 g/pot was more efficient in this concern. Also, chitosan and
thiamine treatments led to an increase in all vegetative and flower traits as
well as photosynthetic pigments and NPK percentages. The treatment of
chitosan at 40 ppm was superior to other used treatments.
The best overall interaction treatment for the best vegetative and
flowering traits was fertilizing plants with compost at 750 g/pot and sprayed
Calendula officinalis with chitosan at 40 ppm.
- 346 -
Abdou, M.A.H. et. al, 2023
- 347 -
Abdou, M.A.H. et. al, 2023
facing the check treatment. Adding 2022/2023 season. The greatest values
compost at 750 g/pot produced the were obtained from plants that received
highest values of all chemical 750 g/pot compost and were sprayed
parameters. with chitosan at a concentration of 40
ppm (in all cases), or thiamine at 50 ppm
These findings are agreed with the
(in some cases) in both seasons.
results mentioned by Hasan et al.
(2014), Khalili et al. (2014) and Abd El- DISCUSSION
Fatah et al. (2019) on pot marigold, El- From our results, it could be
Hindi et al. (2006) and Abdou et al. concluded that all vegetative growth,
(2023) on cineraria, Abdou (2003) on flowering and some chemical parameters
chrysanthemum and Marashi et al. were enhanced as supplying calendula
(2021) on zinnia.
plants grown in sandy soil with compost
Regard to the impact of chitosan and and sprayed with chitosan and thiamine.
thiamine treatments, data given in Tables It may be due to adding compost
(3 and 4) pointed out that all improves soil physio-chemical properties
concentrations of both stimulants (increase CEC, decrease soil pH and
significantly enhanced pigments, N, P improved the most nutrient, structure,
and K% facing the control in both porosity and aggregation), therefore,
experimental seasons. Chitosan at 40 improving plant growth (Snyman et al.,
ppm was more effective than other 1998). Furthermore, chitosan treatments
treatments, followed by thiamine at 50 promote stomatal closure through ABA
ppm. synthesis and photosynthesis, increase
antioxidant enzymes through nitric oxide
Many authors showed that positive
and hydrogen peroxide signaling paths,
effect of chitosan in improving
and induce the creation of sugars, amino
photosynthetic pigments such as Abdel-
acids, organic acids, and further
Mola and Ayyat (2020) and Akhtar et
metabolites required for osmotic stress-
al. (2022) on Calendula officinalis and
related adaptation, stress signaling, and
Mazrou et al. (2021) on Matricaria
energy metabolism (Hidangmayum et
chamomilla, for the effect of chitosan.
al., 2019). In addition, thiamine (vitamin
Similarly for the effect of thiamine was
B1) is required for the formation of the
emphasized by Soltani et al. (2014) on
coenzyme thiamine pyrophosphate,
Calendula officinalis, Abdou et al.
which is vital in glucose metabolism
(2014) and Fallahi et al. (2018) on sweet
basil, Ahmed (2013) on gladiolus plant, (Robinson, 1973; Hendawy and Ezz
EL-Din, 2010), sequence, improved
Al-Abbasi et al. (2015) on Zinnia
growth and flowering productivity.
elegans, and Awad (2019) on borage
plant. CONCLUSION
The influence of the interaction To obtain good growth and flower
between compost and chitosan or production of potted mzrigold plant, it
thiamine treatments had significant effect should be fertilized plants with 750 g/pot
on chl. a, chl. b and carotenoids contents compost and sprayed them with chitosan
in addition to NPK % in both at 40 ppm three times.
experimental seasons, except N % in
- 349 -
Abdou, M.A.H. et. al, 2023
Table (a): The physical and chemical analysis of the soil used in the study.
Table (b): Chemical and physical properties of the used compost in both seasons of
2021/2022 and 2022/2023 as listed in factory label criteria.
Properties Value Properties Value
Organic carbon (%) 25.1 Total P (%) 0.5
Humidity (%) 25 Total K (%) 1.0
Organic matter 44 Fe (ppm) 1750
C/N ratio 17.5 Zn (ppm) 60
pH (1:2.5) 8.0 Mn (ppm) 125
E.C. (m. mhos/cm.) 5.0 Cu (ppm) 200
Total N (%) 1.5
- 350 -
Abdou, M.A.H. et. al, 2023
Table (1): Effect of compost level, some stimulants (chitosan, vitamin B1) treatments
and their interactions on plant height, number of main branches/plant
and plant dry weight of Calendula officinalis in the two growing seasons
(2021/2022 and 2022/2023).
Compost levels treatments (g/pot) (A)
Some stimulants
Mean Mean
treatments 0.0 250 500 750 0.0 250 500 750
(B) (B)
The 1st season (2021/2022) The 2nd season (2022/2023)
Plant height (cm)
Control 26.1 28.7 30.7 32.3 29.5 26.7 29.1 31.1 32.6 29.9
Chitosan at 20 ppm 28.4 30.9 33.1 34.8 31.8 29.1 3p1.7 34 35.7 32.6
Chitosan at 40 ppm 29.0 31.6 33.8 35.5 32.5 29.8 32.4 34.7 36.5 33.4
Thiamine at 25 ppm 27.9 30.4 32.5 34.1 31.2 28.7 31.3 33.5 35.2 32.2
Thiamine at 50 ppm 28.8 31.4 33.6 35.3 32.3 31.06 33.84 36.22 38.04 34.8
Mean (A) 28.0 30.6 32.7 34.4 29.1 31.7 33.9 35.6
L.S.D. at 5 % A: 1.3 B: 1.4 AB: 2.8 A: 0.8 B: 1.6 AB: 3.2
Number of main branches/plant
Control 4.76 5.28 5.73 6.16 5.48 5.11 5.57 5.96 6.26 5.73
Chitosan at 20 ppm 4.79 5.50 5.99 6.30 5.65 5.88 6.40 6.85 7.20 6.58
Chitosan at 40 ppm 5.78 6.54 6.75 7.05 6.53 7.10 7.74 8.27 8.69 7.95
Thiamine at 25 ppm 4.78 5.49 5.94 6.26 5.62 5.80 6.36 6.43 7.09 6.42
Thiamine at 50 ppm 5.69 6.27 6.58 6.91 6.36 6.66 7.25 7.76 8.15 7.46
Mean (A) 5.16 5.82 6.20 6.54 6.11 6.66 7.05 7.48
L.S.D. at 5 % A: 0.30 B: 0.47 AB: 0.94 A: 0.27 B: 0.24 AB: 0.47
Plant dry weight (g)
Control 36.30 39.93 43.51 46.57 41.58 36.79 40.46 44.1 47.2 42.14
Chitosan at 20 ppm 37.18 40.89 44.56 47.69 42.58 38.29 41.39 45.12 48.31 43.28
Chitosan at 40 ppm 38.48 42.35 46.13 49.36 44.08 38.99 42.89 46.75 50.02 44.66
Thiamine at 25 ppm 37.10 40.44 44.42 47.57 42.38 37.72 41.42 44.77 48.22 43.03
Thiamine at 50 ppm 37.77 41.55 45.29 48.46 43.27 38.32 42.16 45.59 49.17 43.81
Mean (A) 37.37 41.03 44.78 47.93 38.02 41.66 45.27 48.58
L.S.D. at 5 % A: 0.74 B: 0.59 AB: 1.18 A: 0.75 B: 0.46 AB: 0.92
- 351 -
Abdou, M.A.H. et. al, 2023
Table (2): Effect of compost level, some stimulants (chitosan, vitamin B1) treatments
and their interactions on total number of flowers/plant and total flower
fresh and dry weights/plant of Calendula officinalis in the two growing
seasons (2021/2022 and 2022/2023).
- 352 -
Abdou, M.A.H. et. al, 2023
Table (3): Effect of compost level, some stimulants (chitosan, vitamin B1) treatments
and their interactions on chlorophyll a, chlorophyll b and carotenoids
content (mg/g f.w.) in fresh leaves of Calendula officinalis in the two
growing seasons (2021/2022 and 2022/2023).
- 353 -
Abdou, M.A.H. et. al, 2023
Table (4): Effect of compost level, some stimulants (chitosan, vitamin B1) treatments
and their interactions on nitrogen, phosphorus and potassium % in dry
leaves of Calendula officinalis in the two growing seasons (2021/2022 and
2022/2023).
- 354 -
Abdou, M.A.H. et. al, 2023
- 355 -
Abdou, M.A.H. et. al, 2023
Babarabie, M.; Zarei, H. AND Badeli, Fadl, M.S. and Sari El-Deen, S.A.
S. (2019). Morphological, (1978). Effect of N-benzyaladenine
physiological and biochemical on photosynthetic pigments and
response of Chrysanthemum to total soluble sugars of olive
thiamine and salicylic acid. Journal seedlings grown under saline
of Plant Physiology and conditions. Res. Bull. Fac. Agric.,
Breeding, 8 (2): 109-120. Ain Shams Univ., 843.
Bistgani, E.Z.; Siadat, S.A.; Fallahi, H.R.; Aminifard, M.H. and
Bakhshandeh, A.; Ghasemi Jorkesh, A. (2018). Effects of
Pirbaluti, A. and Hashemi, M. thiamine spraying on biochemical
(2016). Influence of chitosan and morphological traits of basil
concentration on morpho- plants under greenhouse conditions.
physiological traits, essential oil Journal of Horticulture and
and phenolic content under Postharvest Research, 1 (1): 27–36.
different fertilizers application in
Ghehsareh, M.G.; Khosh-Khui, M.
Thymus daenensis. Journal of and Nazari, F. (2011). Comparison
Medicinal Herbs., 7 (2): 117-125.
of municipal solid waste compost,
Chen, Y.E.; Yuan, S.; Liu, H.M.; vermicompost and leaf mold on
Chen, Z.Y.; Zhang, Y.H. and growth and development of
Zhang, H.Y. (2016). A cineraria (Pericallis× hybrida ‘Star
combination of chitosan and Wars’). Journal of Applied
chemical fertilizers improves Biological Sciences, 5 (3): 55-58.
growth and disease resistance in
Golestani, M.; Dolatkhahi, A. and
Begonia× hiemalis
Kazemi, F. (2013). Effect of
Fotsch. Horticulture, Environment,
planting dates on flowering period
and Biotechnology, 57, 1-10.
of Calendula officinalis, Bellis
http://0.1007/s13580-016-0119-4
perennis and Viola sp. Advanced
El-Hindi, K.; El-Shikha, O.M. and El- Crop Science. 3 (8): 563-567.
Ghamry, A.M. (2006). Response
Gupta, R.; Yadav, A. and Garg, V.K.
of cineraria plant to water stress
(2014). Influence of vermicompost
and compost sources under drip
application in potting media on
irrigation system. Journal of Plant
growth and flowering of marigold
Production, 31 (5): 3129-3146.
crop. International Journal of
El-Sirafy, Z.M.; El-Hamdi, K.H.; Recycling of Organic Waste in
Taha, A.A. and Abdel-Naby, Agriculture, 3: 1-7.
H.M. (1989). Pepper production on www.http://10.1007/s40093-014-
sandy soil as affected by compost 0047-1
addition and nitrogen Hasan, F.A. (2013). Effect of spraying
fertilization. J. Agric. Sci., with thiamin B1 and salicylic acid
Mansoura Univ., 14: 1793-1802.
in vegetative and flowering of
Calendula officinalis L. plant. Thi-
- 356 -
Abdou, M.A.H. et. al, 2023
- 357 -
Abdou, M.A.H. et. al, 2023
Soltani, Y.; Saffari, V.R. and hakur, R.; Dubey, R.K.; Kukal, S.S.
Maghsoudi Moud, A.A. (2014). and Sammy, K. (2016). Interactive
Response of growth, flowering and effect of biofertilizers and organic
some biochemical constituents of potting media on growth and
Calendula officinalis L. to foliar flowering of calendula (Calendula
application of salicylic acid, officinalis Linn.). Indian Journal of
ascorbic acid and thiamine. Ethno- Ecology, 43 (1): 245-248.
Pharmaceutical products, 1: 37-44.
- 359 -
Abdou, M.A.H. et. al, 2023
الملخص العربي
2
محمىد عبذالهادي حسه عبذه– 1رجاء علي طه –1شيماء أحمذ حسه- 1عبذهللا محمذ عثمان جهىري
أُخزٌ هذا انعًم فٍ يشخم َببحبث انشَُت ،قسى انبسبحٍُ ،كهُت انشراعت ،خبيعت انًُُب ،خالل يىسًٍ انًُى
2022/2021و ،2023/2022الخخببر حأثُز يسخىَبث انكًبىسج (صفز – 050 – 500 – 250خى/أصُص)
وانشُخىساٌ ( 20و 40خشء فٍ انًهُىٌ) وانثُبيٍُ ( 250و 50خشء فٍ انًهُىٌ) وانخذاخم بُُهى عهٍ صفبث انًُى
وانخشهُز وبعض انًكىَبث انكًُبوَت نُببث األقحىاٌ.
أظهزث انُخبئح أٌ خًُع صفبث انًُى انخضزٌ (طىل انُببث –عذد انفزوع انزئُسُت/انُببث –انىسٌ اندبف
نهُببث) وصفبث انًُى انشهزٌ (انعذد انكهٍ نهُىراثَ/ببث – انىسٌ انكهٍ انطبسج واندبف نألسهبر/انُببث) وبعض
انًكىَبث انكًُبوَت (صبغبث انبُبء انضىئٍ – انُسبت انًئىَت نهُُخزوخٍُ وانفىسفىر وانبىحبسُىو) سادث سَبدة يعُىَت
َخُدت سَبدة يسخىَبث انكًبىسج فٍ يقببم يعبيهت انكُخزول .وكبَج انًعبيهت 050خى/أصُص كًبىسج هٍ األكثز
فعبنُت فٍ هذا انشأٌ .أَضبً ،أدث يعبيالث انشُخىساٌ وانثُبيٍُ إنٍ سَبدة خًُع صفبث انًُى انخضزٌ وانشهزٌ وكذنك
انصبغبث وانُسبت انًئىَت نهُُخزوخٍُ وانفىسفىر وانبىحبسُىو ،وكبَج يعبيهت انشُخىساٌ عُذ 40خشء فٍ انًهُىٌ يخفىقت
عهً خًُع انًعبيالث األخزي.
كبَج أفضم يعبيهت حفبعم نهحصىل عهٍ أفضم انصفبث انخضزَت وانشهزَت هٍ حسًُذ َببث األقحىاٌ
ببنكًبىسج بـ 050خى/أصُص ورش انُببث ببنشُخىساٌ عُذ حزكُش 40خشء فٍ انًهُىٌ.